Low Tolerance Research Articles

Improved mechanical and fire-retardant properties of fiber-reinforced composites manufactured via modified resins and metallic thin films

Fiber-reinforced polymeric composites have been extensively used in different industrial applications because of their excellent mechanical and other properties but have lower tolerance levels for fire and lightning damage. The thermal, mechanical, and electrical conductivity of these composites can be substantially increased using some thin metallic films for higher fire resistance. The objective of this study was to develop fire-retardant fiber-reinforced composites using modified resins and metallic copper (Cu) thin films and test and characterize the mechanical and thermal properties of these prepared composites. Standard hand wet layup process was used to manufacture composite panels, and then the flame retardant and other physical and chemical properties were determined before and after resin modification and surface metal film coatings. These modified resins and the conductive metallic films of the composite provided superior flame retardancy and higher mechanical strength. The prepared composite panels made from modified epoxy via 9,10-dihydro-9-oxo-10-phosphaphenanthrene-10-oxide (DOPO) inclusion and with metallic surface coatings passed the UL-94 vertical flame testing with a V-0 rating. This composite achieved an average flexural strength of 344.2 MPa, a mean tensile strength of 400.82 MPa, and a shear strength of 6.54 MPa for single lap shear joint studies. Fractography results also show better bonding of the matrix and fiber with no significant damage. This study may open new opportunities in various composite industries.

An analysis of Risk Tolerance of Individual Investor using Gray Relational Analysis Method

Risk tolerance refers to an individual investor's inclination towards embracing fluctuations in investment values in pursuit of higher income. It reflects their capacity to endure such fluctuations and financial goals, time horizon and emotional comfort various including status influenced by factors. Personal preferences and investment choices with circumstances as it helps to regulate, informed investment decisions one's risk tolerance in taking understanding is important. financial situation, investment objectives, knowledge of the financial market, factors like experience, and emotional intelligence contribute to determining one's risk tolerance. Investors' risk tolerance is often assessed through questionnaires or evaluations conducted by financial advisors, which consider factors like attitudes towards risk, investment goals, time horizon, and financial situation. Based on the assessment, investors may be categorized into different risk profiles such as conservative, moderate, or aggressive, with corresponding investment strategies suggested accordingly. It's essential for investors to honestly evaluate and be realistic about their risk tolerance, as overestimating it may lead to excessive risk-taking and potential losses during market downturns, while underestimating it may result in overly conservative investment choices. Regular reviews of risk tolerance are recommended, especially in light of significant life events or market fluctuations. The concept of risk-return trade-off highlights that higher returns are typically associated with higher levels of risk, implying that investors expecting higher returns must be prepared to accept greater potential losses. However, risk tolerance isn't solely determined by financial factors; emotional elements such as fear, anxiety, and market sentiment also influence an investor's tolerance for risk and their behaviour during periods of volatility. Therefore, a comprehensive understanding of risk tolerance encompasses both financial considerations and emotional factors. the GRA (Grey Relational Analysis) approach at its inception, focusing on the concept of the gray gadget. This technique is particularly effective for selection problems involving multiple attributes within a component. The current literature highlights its applicability in addressing problems associated with multiple factors and variables, especially when dealing with complex relationships. The GRA approach is well-suited for resolving issues related to fixing problems, and various types of GRA techniques have been proposed in the field. The introduction of the GRA approach is both straightforward and environmentally friendly, making it a practical choice for addressing complex problems involving multiple variables. Investment Objectives, Time Horizon, Financial Situation, Knowledge and Experience and Liquidity Needs. Risk Assessment Questionnaires, Investment Experience and Knowledge, Financial Situation, Investment Goals and Emotional Attitude Towards Risk. the Rank of GRA for Risk Tolerance of Individual Investors. Liquidity Needs is got the first rank whereas the Low Risk Tolerance is having the Lowest rank.

Utilizing Euler poles for the evaluation of plate rigidity in numerical mantle convection models

SUMMARY Evidence that the Earth’s surface is divided into a tessellation of piece-wise rigidly translating plates is the primary observation supporting the solid-state creep-enabled convection paradigm, utilized to investigate evolution of the Earth’s mantle. Accordingly, identifying the system properties that allow for obtaining dynamically generated plates remains a primary objective in numerical global mantle convection simulations. The first challenge for analysing fluid dynamic model output for the generation of rigid plates is to identify candidate plate boundaries. Here, we utilize a previously introduced numerical tool for plate boundary detection which uses a user specified threshold (tolerance) to automatically detect candidate plate boundaries. The numerical tool is applied with different sensitivities, to investigate the nature of the surface velocity fields generated in three calculations described in earlier work. The cases examined differ by the values that they specify for the model yield stress, a parameter that can allow the formation of tightly focussed bands of surface deformation. The three calculations we examine include zones comprising possible plate boundaries that are characterized by convergence, divergence and strike-slip behaviour. Importance of the potential plate boundaries is assessed by examining the rigidity of the inferred model generated plates. The rigidity is measured by comparing the model velocities to the rigid rotation velocities implied by the statistically determined Euler poles for each candidate plate. We quantify a lack in rigidity by calculating a deformity field based on disagreement of actual surface velocity with rotation about the Euler pole. For intermediate yield stress and boundary detection threshold value, we find that the majority of the model surface can translate almost rigidly about distinct plate Euler poles. Regions that conform poorly to large-scale region rigid translation are also obtained but we find that generally these regions can be decomposed into subsets of smaller plates with a lower tolerance value. Alternatively, these regions may represent diffuse boundary zones. To clarify the degree to which the mantle convection model behaviour shows analogues with Earth’s current-day surface motion, we apply the plate boundary detection and Euler pole calculation methods to previously published terrestrial strain-rate data. Strong parallels are found in the response of the terrestrial data and mantle convection calculations to the threshold value, such that appropriate choice of that parameter results in very good agreement between observations and convection model character. We conclude that plates generated by fluid dynamic convection models can exhibit motion that is markedly rigid, and define statistics (plateness) and fields (deformity) by which the generation of self-consistently determined plate rigidity can be quantified, as well as describing how plate recognition might be optimized. We also note that in agreement with the Earth’s current state, we obtain a dozen dominant plates in the case exhibiting the most plate-like (rigid) surface, suggesting that this approximate number of plates is perhaps intrinsic to the geometry, surface area and physical properties of Earth’s mantle.

Climate change in Europe and international portfolio allocation: Micro‐level evidence from global funds

AbstractThis paper investigates the impact of climate change on international portfolio allocations of global funds. We select the heat wave disasters in Europe in 2018 as a quasi‐natural experiment and employ the DID framework. Our findings indicate that global funds would reduce their holdings in the affected country after heat wave disasters. The congestion of inland waterways and electricity shortages during the heatwave have resulted in production delays, thereby transmit risks to the financial sector. Countries with higher foreign exposure experience a larger scale of capital outflow after heat waves; while countries with great institutional quality and resilience demonstrate greater capacity to withstand heat wave disasters. The funds with lower risk tolerance are more sensitive to climate change. Additionally, domestic funds will remain in their home countries post‐heatwave when foreign capitals flow away. These findings have important policy implications, and countries with high climate risks should carefully monitor the capital flows. In order to reduce the impact of climate risks, it is crucial to improve the quality of institution and efficiency of government as well as to implement effective disaster prevention and mitigation measures.

Transgenerational cross‐susceptibility to heat stress following cold and desiccation acclimation in the Angoumois grain moth

AbstractThe Angoumois grain moth, Sitotroga cerealella (Olivier), is a significant cosmopolitan primary pest of cereals worldwide and has thrived in divergent environments. However, the mechanisms underlying its survival in multiple contrasting environments are poorly understood. Here, we hypothesised that when facing diverse environmental stress, F1 generation exhibits transgenerational cross‐protection as a mechanism to persist under divergent stressful environments. Notably, F1 acclimation to cold or desiccation conditions could either enhance or lower heat tolerance in the F2 generation. Specifically, we tested whether typical diurnal fluctuations and/or winter rapid or chronic cold temperatures (18–22°C) as well as desiccation acclimation of F1 parental population yields transgenerational cross‐protection/susceptibility to heat stress on F2 offspring. F1 moths were exposed to cold hardening (2 h), chronic (72 h) and variable (fluctuating between 18 and 22°C for 72 h) temperature treatment groups. Desiccation treatment included incubation at 0%–1% relative humidity (24 h). F2 generation moths were then assessed for heat tolerance using critical thermal maxima (CTmax) at three different ramping rates (0.06, 0.25 and 0.5°C/min) as well as heat knockdown time (HKDT). Findings indicated that (i) desiccation, cold hardening and chronic low‐temperature acclimations in F1 reduced heat tolerance in F2 populations and (ii) ramping rate was crucial to decipher differences between treatment groups, with lower ramping rates associated with lower CTmax. Transgenerational cross‐susceptibility to heat stress indicates possible fitness costs of exposure to multiple contrasting stressors in the Angoumois grain moth and can be used in designing physical pest management strategies.

Axiological criteria for predicting the success of socio-psychological adaptation of schoolchildren of younger adolescence

Introduction. The system of personal value orientations is considered the main determinant of the processes of socio-psychological adaptation. The value structure has a significant impact on both the development and formation of a personality, as well as on the regulation of its self-awareness, cognitive and emotional sphere and behavior. In adolescence, the maximum stress of adaptation processes occurs and the most pronounced intolerance to negative environmental influences is manifested. In this regard, the analysis of value-intentional mechanisms and strategies of sociopsychological adaptation at this age seems extremely relevant.Materials and Methods. The study involved 300 boys and girls of early adolescence, who, using a refined technique, had Schwartz and the K. Rogers and R. Diamond questionnaire "Sociopsychological adaptation" assessed the system of value orientations and the level of adaptation. Statistical processing of the materials included the calculation of the Mann-Whitney U-test and discriminant analysis (step-by-step Wilkes method).Results. The system of value orientations (includes 19 parameters) and the level of social adaptation of adolescents are studied. The comparison of value and adaptation parameters in boys and girls was carried out. Using statistical procedures, respondents with high and low levels of adaptability were identified. The comparison of value indicators was carried out both between respondents with different degrees of adaptation, and between adolescents of different genders. Canonical functions with a high discriminative ability for boys and girls are calculated, which allow using axiological variables to divide the respondents according to their degree of social adaptation.Discussion and Conclusions. It is shown that the effectiveness of the processes of social adaptation of adolescents improves as the degree of differentiation of value orientations increases, manifested by an increase in the "spread" of their positions in the "field" of value consciousness. It is established that the most important factor in the successful social adaptation of adolescents is the dominance of the main group-centered value orientations and the diminution of the role of egocentric intentions. The ambivalence of the influence of hedonistic intention on adaptation has been revealed, the increasing importance of which, in combination with the development of groupcentered values, contributes to successful social adaptation, and in combination with the increasing role of other egocentric values, becomes a factor of low tolerance to social frustration. The maladaptive influence of the value orientations "reputation" (in boys) and "independence" (in girls) on the processes of social adaptation has been established. The axiological criteria are calculated.

Efficacy and safety of eight enhanced therapies for treatment-resistant depression: A systematic review and network meta-analysis of RCTs

BackgroundTreatment-Resistant Depression (TRD) challenges psychiatric treatment, with existing guidelines covering only a subset of augmentation strategies. MethodsA network meta-analysis following PRISMA guidelines examined the efficacy and safety of TRD treatments, analyzing 72 randomized controlled trials from eight databases, assessing response and remission rates, tolerability, and safety through the Cochrane Risk of Bias Tool and CINeMA framework. FindingsIncluding 12,105 participants, the analysis highlighted ECT, Ketamine, Esketamine, and Psilocybin as superior first-line treatments due to their optimal balance between effectiveness and tolerability. Brexpiprazole and Quetiapine showed no significant efficacy over placebo in response rates, while Esketamine and Psilocybin exhibited lower tolerability. InterpretationThe results advocate for ECT, Ketamine, Esketamine, and Psilocybin as preferred treatments for TRD, guiding clinical practice with evidence-based recommendations for enhancing treatment outcomes. This study underscores the importance of considering both efficacy and safety in selecting augmentation strategies for TRD.

Religious Moderation Perspectives and Pratices in Muslim Youths With International Exposure

Travel across border give opportunity to people to immerse in a diverse groups and societies. It is hoped to enrich life experiences that lays the groundwork for the creation of more harmonious and tolerant societies. Religious moderation, especially in Indonesian context, require tolerance, anti-violence, the sense of nationality and local culture acceptance from its citizen to maintain the harmonious, moreover, the unity of Indonesia as a country. Unfortunately, some Muslim youths were found to have low tolerance toward different religion groups. This study aims to assess if Muslim youths with international exposure have good moderation perspectives and practices in religion, including tolerance. This study is a quantitative descriptive with the help of survey tool to collect data from Muslim youths with international experiences. The analysis was carried out using univariate analysis. The results depict that more than 90% of the 155 respondents have good perspectives and practices toward religious moderation. Only practice of tolerance has slightly lower percentage which is 89%. More international programs for Muslim youths are encouraged to help them increase the level of tolerance toward differences

Temperature-induced wear micro-mechanism transition in additively deposited nickel alloys with different solid lubricants

Additive manufacturing of self-lubricating alloys plays a crucial role in the production of complex wear-resistant components and in expanding repair capabilities, especially for intricate wear parts with low tolerances (e.g. with cooling channels). Herein, we report a novel approach providing nickel-based alloy with an excellent tribological performance in dry sliding contacts. Laser-deposited self-lubricating nickel alloys, infused with anti-wear additives of molybdenum disulfide, nickel sulfide, copper sulfide, or bismuth sulfide, were subjected to dry sliding wear tests against an alumina ball counterbody at a temperature range of up to 800 °C. The self-lubricating alloys exhibited a significant decline in friction (43 %) and wear (45 %) at room temperature, 400 °C (friction 40 %, wear rate 55 %), and 600 °C (MoS2-based, friction 58 %, wear rate 75 %). The MoS2-based alloy coating demonstrated excellent performance characteristics up to 800 °C (friction coefficient ⁓0.25, wear rate 11 × 10−6 mm3 N−1 m−1) due to the formation of a ‘glazed’ tribolayer. Wear mapping allowed to identification of a critical condition for self-lubricating alloys where positive transitions in wear mechanisms led to a synergistic lubrication mode involving the formation of tribologically induced new lubricious phases such as silver molybdate or nickel-bismuth intermetallic. This work provides a comparative evaluation of the micromechanisms, surface transitions, and tribochemistry of solid lubricants at a wide temperature range and a variety of applications.

How do urban trees vary across the US? It depends on where and how you look

Urban forests provide ecosystem services important for regulating climate, conserving biodiversity, and maintaining human well‐being. However, these forests vary in composition and physiological traits due to their unique biophysical and social contexts. This variation complicates assessing the functions and services of different urban forests. To compare the characteristics of the urban forest, we sampled the species composition and two externally sourced traits (drought tolerance and water‐use capacity) of tree and shrub species in residential yards, unmanaged areas, and natural reference ecosystems within six cities across the contiguous US. As compared to natural and unmanaged forests, residential yards had markedly higher tree and shrub species richness, were composed primarily of introduced species, and had more species with low drought tolerance. The divergence between natural and human‐managed areas was most dramatic in arid climates. Our findings suggest that the answer to the question of “what is an urban forest” strongly depends on where you look within and between cities.

Genome‐Wide Identification of CLE Gene Family and Function Analysis of SbCLE39 Under Salt Stress in Sorghum

ABSTRACTCLE proteins are a class of signalling factors involved in plant growth and abiotic stress response. They play crucial roles in processes such as cell differentiation, chlorophyll synthesis and abscisic acid (ABA) signal transduction. However, the function of the CLE genes in Sorghum bicolor remains unclear. In this study, 42 sorghum CLE genes were identified, and their evolutionary relationship, gene structure, amino acid sequence and homologous genes were analysed. We also examined the expression levels of CLE genes under various treatment conditions. Transcriptome data showed that there were significant differences in the expression patterns of 42 CLE genes in different tissues and organs. It is worth noting that SbCLE39 is mainly highly expressed in sorghum roots. At the same time, the expression of SbCLE39 decreased significantly under salt and ABA treatment. Compared with wild‐type yeast cells (EV), yeast cells with high expression of SbCLE39 had lower tolerance to salt stress. In addition, the excessive accumulation of ABA caused by external application of SbCLE39p reduced the salt tolerance of sorghum. These findings suggest that SbCLE39 negatively regulates the salt tolerance of sorghum. These results lay a foundation for revealing the mechanism of CLE genes regulating the salt tolerance of sorghum and are of great significance for the cultivation of salt‐tolerant crops.

OsJRL negatively regulates rice cold tolerance via interfering phenylalanine metabolism and flavonoid biosynthesis.

The identification of new genes involved in regulating cold tolerance in rice is urgent because low temperatures repress plant growth and reduce yields. Cold tolerance is controlled by multiple loci and involves a complex regulatory network. Here, we show that rice jacalin-related lectin (OsJRL) modulates cold tolerance in rice. The loss of OsJRL gene functions increased phenylalanine metabolism and flavonoid biosynthesis under cold stress. The OsJRL knock-out (KO) lines had higher phenylalanine ammonia-lyase (PAL) activity and greater flavonoid accumulation than the wild-type rice, Nipponbare (NIP), under cold stress. The leaves had lower levels of reactive oxygen species (ROS) and showed significantly enhanced cold tolerance compared to NIP. In contrast, the OsJRL overexpression (OE) lines had higher levels of ROS accumulation and showed lower cold tolerance than NIP. Additionally, the OsJRL KO lines accumulated more abscisic acid (ABA) and jasmonic acid (JA) under cold stress than NIP. The OsJRL OE lines showed increased sensitivity to ABA compared to NIP. We conclude that OsJRL negatively regulates the cold tolerance of rice via modulation of phenylalanine metabolism and flavonoid biosynthesis.

Yield -based evaluation of low nitrogen tolerance indices for screening of [Sorghum bicolor (L.) Moench] genotypes

Nitrogen stress is one of the key factors limiting sorghum crop productivity. To identify desirable sorghum genotypes best suited to nitrogen stress, the response of 300 sorghum landraces and two standard checks: KS78, and BCK60 were evaluated using yield-based indices. The experiment was conducted under nitrogen stress and ideal conditions for two years using a split-plot in RCBD design with two replications. Nitrogen treatments were assigned to the main plots, and sorghum genotypes were assigned to the subplots. Fourteen nitrogen stress screening indices, namely, the yield stability index (YSI), stress tolerance index (STI), modified stress tolerance index (KSTI), low nitrogen tolerance index (LNTI), relative decrease in yield (RDY), tolerance index (TOL), yield index (YI), stress susceptibility index (SSI), stress susceptibility percentage index (SSPI), and harmonic mean (HM), were computed using grain yield. The yield under optimal and stress conditions was significantly positively correlated with the HM, MP, GMP, STI, HM, YI, and modified STI. The results from the cluster analysis, PCA biplot, and 3D biplot classified the genotypes into three groups: nitrogen stress tolerant, nitrogen stress susceptible, and poor performers under both environments. Using STI, GMP, and MP, Genotypes (G80) ETSL101255, (G216) ETSL101006, (G35) ETSL101258, (G146) ETSL100813, and genotypes (G244) ETSL100292, (G161) ETSL100539, (G240) ETSL100890, (G93) ETSL101845, (G261) ETSL100625, (G248) ETSL100874, (G258) ETSL100752, (G142) ETSL100921, (G135) ETSL100307 were identified as top nitrogen stress tolerant, and susceptible genotypes respectively. We conclude that the indices MP, GMP, and STI can help in improving sorghum yield by selecting nitrogen efficient genotypes.

Exploring the Cellular Impact of Size-Segregated Cigarette Aerosols: Insights into Indoor Particulate Matter Toxicity and Potential Therapeutic Interventions.

Exposure to anthropogenic aerosols has been associated with a variety of adverse health effects, increased morbidity, and premature death. Although cigarette smoke poses one of the most significant public health threats, the cellular toxicity of particulate matter contained in cigarette smoke has not been systematically interrogated in a size-segregated manner. In this study, we employed a refined particle size classification to collect cigarette aerosols, enabling a comprehensive assessment and comparison of the impacts exerted by cigarette aerosol extract (CAE) on SH-SY5Y, HEK293T, and A549 cells. Exposure to CAE reduced cell viability in a dose-dependent manner, with organic components having a greater impact and SH-SY5Y cells displaying lower tolerance compared to HEK293T and A549 cells. Moreover, CAE was found to cause increased oxidative stress, mitochondrial dysfunction, and increased levels of apoptosis, pyroptosis, and autophagy, leading to increased cell death. Furthermore, we found that rutin, a phytocompound with antioxidant potential, could reduce intracellular reactive oxygen species and protect against CAE-triggered cell death. These findings underscore the therapeutic potential of antioxidant drugs in mitigating the adverse effects of cigarette aerosol exposure for better public health outcomes.

Ambivalent Sexism and Tolerance of Violence Against Women in India

We examined associations between sexist beliefs and tolerance of violence against women in India using a nationally representative probability sample of adults (n = 133,398). Research consistently indicates that hostile sexism fosters tolerance of violence against women. However, benevolent sexism is sometimes associated with higher tolerance and sometimes with lower tolerance of violence. We proposed that this inconsistency could be resolved by considering the source of violence: Is violence perpetrated by outsiders or intimate partners? Results of a multigroup structural equation model showed that endorsement of hostile sexism was related to greater tolerance of violence regardless of the source. In contrast, endorsement of benevolent sexism was associated with lower tolerance of violence from outsiders but was simultaneously associated with higher tolerance of spousal violence. These opposing processes indicate that although benevolent sexism promises women protection from violence, the very same ideology legitimizes spousal violence, thereby reinforcing men’s power within intimate relationships.

Insights into the physiology, biochemistry, and metabolic pathways of ethanol-resistant marine microalgae in phytoplankton

Over two decades have passed since genetically modified prokaryotic cyanobacteria became capable of photosynthetically producing bioethanol. However, the limitation of low ethanol tolerance has impeded bioethanol production. In this study, the findings revealed that eukaryotic marine microalgae exhibited higher tolerance to ethanol compared to freshwater microalgae through a comparison of the growth performance of algal species under ethanol-induced stress. Specifically, the marine microalga Chlorella sp. MEM17 (MEM17) demonstrated tolerance to ethanol concentrations up to 6.0 % (v/v). Additionally, the polysaccharide and unsaturated fatty acid contents of MEM17 were considerably higher compared to ethanol-untreated control groups under ethanol stress. In conjunction with the comparative metabolomics approach, the remodeling of glycerophospholipid metabolic pathways was observed, specifically the conversion of a fraction of the reduced glycerophospholipids into specific glycerophospholipids, thereby enhancing the hindrance of ethanol entry into cells. The up-regulation of amino acid-related metabolic pathways aimed to augment the relative content of specific metabolites, such as gamma-aminobutyric acid (GABA), with the objective of scavenging intracellular reactive oxygen species (ROS). Furthermore, bolstered properties of the cell wall potentially facilitated by the increased polysaccharide content of MEM17 further impeded the potential entry of ethanol into the cell, while excess polysaccharide assisted in scavenging intracellular ROS. This study represents the first discovery of a high-concentration ethanol-tolerant eukaryotic marine microalga and its key metabolic pathways, with potential as “marine photosynthetic cell factories” for bioethanol production.

Nanobody and CuS Nanoflower-Au-Based Lateral Flow Immunoassay Strip to Enhance the Detection of Aflatoxin B1.

In the realm of analysis, the lateral flow immunoassay (LFIA) is frequently utilized due to its capability to be fast and immediate. However, the biggest challenge of the LFIA is its low detection sensitivity and tolerance to matrix interference, making it impossible to enable accurate, qualitative analyses. In this study, we developed a new LFIA with higher affinity and sensitivity, based on a nanobody (G8-DIG) and CuS nanoflowers-Au (CuS NFs-Au), for the detection of aflatoxin B1 (AFB1) in maize. We synthesized the immunoprobe G8-DIG@CuS NFs-Au, stimulated the in situ development of Au nanoparticles (Au NPs) on Cu NFs by electrical displacement, and obtained Cu NFs-Au for fixing the G8-DIG. G8-DIG@CuS NFs-Au probe-based LFIAs may, in ideal circumstances, use a strip chromatography reader to accomplish sensitive quantitative detection and qualitative visualization. AFB1 has a detection range of 2.82-89.56 µg/L and a detection limit of 0.87 µg/L. When compared with an LFIA based on CuS NFs, this sensitivity is increased by 2.76 times. The practical application of this method in corn flour demonstrated a recovery rate of 81.7% to 117%. Therefore, CuS NFs-Au show great potential for detecting analytes.

DNA nanoassembly based turn-on amplification probe for sensitive colorimetric CRISPR/Cas12a-mediated detection of pathogen DNA

Clustered regularly interspaced short palindromic repeat (CRISPR) system has been explored as an efficient tool for nucleic acid diagnostics. However, it normally needs instrumentation or produces turn-off signals. Herein, a bulged Y-shape DNA (Y-DNA) nanoassembly was designed and synthesized as a novel turn-on probe. A CRISPR/Cas12a and Y-DNA probe mediated colorimetric assay (named as CYMCOA) strategy was developed for visual detection of pathogen DNA. Upon activating Cas12a with pathogen DNA, the Y-DNA bulge is catalytically trans-cleaved, releasing the G-quadruplex sequence embedded in the Y-DNA nanoassembly as a peroxidase-like DNAzyme. Visible signals with chromogen substrates are thus produced. The CYMCOA strategy was combined with recombinase polymerase amplification (RPA), an isothermal amplification technique, in detecting Helicobacter pylori (Hp) bacteria and SARS-CoV-2 N plasmids as two model pathogens. The bioassay has very excellent detection sensitivity and specificity, owing to the triple cascade amplification reactions and the very low mismatch tolerance. The lower limit of detection values were 0.16 cfu⋅mL−1, 1.5 copies⋅μL−1, and 0.17 copies⋅μL−1 for Hp bacteria, Hp plasmids, and SARS-CoV-2 N plasmids respectively. The detection is fast and accurate. The colorimetric bioassay strategy provides to be a simple, accurate, fast and instrumentation-free platform for nucleic acids detections in various settings, including crude and emergent situations.

Smoking patterns and the intention to quit in German cancer patients: a cross-sectional study

BackgroundContinued smoking after a cancer diagnosis can be associated with lower treatment tolerance, poorer outcomes, and reduced quality of life compared to non-smoking cancer patients or to those who have quit. Yet about 60% of patients continue to smoke after being diagnosed and find it difficult to quit. To address this problem, it is necessary to identify current and past smoking patterns (e.g., frequency of use, types of tobacco products) and determine whether there is motivation to quit. Similarly, factors associated with continued smoking should be identified. These data will provide the basis for the development of smoking cessation programs tailored to the needs of cancer patients.MethodsA questionnaire was distributed to cancer patients older than 18 years in a German Comprehensive Cancer Center. Participating cancer patients were divided into three main groups: 1) patients who stopped smoking before being diagnosed with cancer (Ex-before); 2) patients who stopped smoking after a cancer diagnosis (Ex-after); and 3) patients who currently smoke cigarettes (CS). Sociodemographic, medical, and psychosocial data were collected, as well as smoking patterns and the motivation to quit smoking.ResultsAbout half of patients (51%) who smoked before diagnosis continue to smoke after a cancer diagnosis. Being diagnosed with a tobacco-related cancer type was associated with a decreased probability of continued smoking. Patients with tobacco-related tumors and receiving positive support in burdensome situations were more likely to have a higher cigarette dependence. Of all CS, 59.1% had intention to quit, and 22.7% reported having taken action to quit.The support by a smoking cessation program was considered important. CS were willing to spend up to €100 for support and were open to multiple sessions per week, group sessions, one-on-one sessions and/or online support.ConclusionThese findings underscore the importance of educating cancer patients about the consequences of smoking and to provide them with support to quit. Identified risk factors may further help to recognize cancer patients with high risk of continued smoking after diagnosis.Trial RegistrationThe study was registered at OSF (https://osf.io/3c9km) and published as a study protocol at “https://bmjopen.bmj.com/content/13/4/e069570”.

One-Pot Two-Stage Biocatalytic Cascade to Produce l-Phosphinothricin by Two Enantioselective Complementary Aminotransferases at High Substrate Loading via a Deracemization Process.

The bioderacemization of racemic phosphinothricin (D, L-PPT) is a promising route for the synthesis of l-phosphinothricin (L-PPT). However, the low activity and tolerance of wild-type enzymes restrict their industrial applications. Two stereocomplementary aminotransferases with high activity and substrate tolerance were identified in a metagenomic library, and a one-pot, two-stage artificial cascade biocatalytic system was developed to produce L-PPT through kinetic resolution and asymmetric amination. We observed that 500 mM D, L-PPT (100 g/L) could be converted into L-PPT with 94% final conversion and >99.9% enantiomeric excess (e.e.) within 24 h, with only 0.02 eq amino acceptor pyruvate and 1.2 eq amino donor l-aspartate required. The process could be scaled up to 10 L under sufficient oxygen and stirring. The superior catalytic performance of this system provides an eco-friendly and sustainable approach to the industrial deracemization of D, L-PPT to L-PPT.