Low Temperature Season Research Articles

Temperature monitoring test and thermal analysis during construction of deep orifice of arch dam

In the low-temperature season of Southwest China, concrete of orifice is easy to crack during pouring, and the superficial heat preservation of dam is needed. In this paper, the temperature gradient monitoring test of deep orifice of Baihetan Dam is carried out to obtain measured temperature data based on the distributed optical fiber sensing technique. Based on monitoring data, a finite element model of deep orifice of pouring blocks considering thermal insulation materials is established. By comparing calculated temperature with measured temperature, accuracy of the simulation model is verified. The temperature and stress of deep orifice with or without thermal insulation measures are analyzed, considering the thermal insulation effects of different thicknesses and different types of thermal insulation materials. The results show that when the insulation measures are not covered, the average temperature difference between day and night exceeds 2 °C, and the average temperature difference between day and night is within 1 °C after coverage. Using 3 cm or 5 cm polyethylene coil at the top surface and the transverse joint surface of pouring blocks, and spraying 3 cm or 5 cm polyurethane at the upstream surface of pouring blocks for thermal insulation can effectively prevent the temperature cracks.

Global patterns of taxonomic and phylogenetic endemism in liverwort assemblages

Exploring the worldwide patterns of endemism and the processes that lead to the formation of high-endemism centers is crucial in biogeography. This study examines the geographic distribution and ecological influences on the endemism of liverworts across 390 regions worldwide. We assess phylogenetic endemism and relative phylogenetic endemism in relation to eleven environmental factors, which represent current and Quaternary climate variations, as well as topographic and environmental heterogeneity. Areas with higher endemism in liverworts tend to have higher temperatures, precipitation, and environmental heterogeneity, but lower temperature seasonality and lesser impacts from Quaternary climate changes. Regions exhibiting notably high endemism are predominantly found in tropical Asia, Madagascar, eastern Australia, and the Andes, while those with notably low endemism are generally in temperate Eurasia and North America, parts of Africa, and eastern South America. Centers of neo-endemism are mainly in southern Africa, whereas centers of paleo-endemism are in southern South America, tropical Asia, and New Zealand. Environment variability is a more significant predictor of phylogenetic endemism than current climate conditions, which are themselves more predictive than variables related to Quaternary climate changes. Nevertheless, these three types of explanatory variables combined explain only about one-third of the variance in phylogenetic endemism.

Preparation of high-temperature and low-temperature-resistant solid microbial agent for cattle manure fermentation and effect on composting.

We used microbiology and molecular biology techniques to screen out high-temperature and low-temperature-resistant saprobiotics for compost and prepared a compound fermentation bacteria agent to rapidly ferment cattle manure into high-quality organic fertilizer in low-temperature season. Conventional composting and high-throughput techniques were used to analyze the changes of physical and chemical indexes and biodiversity in the process of composting, from which high and low-temperature-resistant strains were obtained, and high-temperature and low-temperature-resistant solid composite bactericides were prepared and added to composting to verify the effects of composite bactericides on composting. The conventional composting cycle took 22days, and the diversity of microflora increased first and then decreased. Composting temperature and microbial population were the key factors for the success or failure of composting. Two strains of high-temperature-resistant bacteria and six strains of low-temperature-resistant bacteria were screened out, and they were efficient in degrading starch, cellulose, and protein. The high-temperature and low-temperature-resistant solid bacterial agent was successfully prepared with adjuvant. The preparation could make the compost temperature rise quickly at low temperature, the high temperature lasted for a long time, the water content, C/N, and organic matter fell quickly, the contents of total phosphorus and total potassium were increased, and the seed germination index was significantly improved. Improve the composting effect. The solid composite bacterial agent can shorten the composting time at low temperature and improve the composting efficiency and quality.

Direct development in Atlantic Forest anurans: What can environmental and biotic influences explain about its evolution and occurrence?

Different environmental and biological factors can originate and support different alternative life histories in different taxonomic groups. Likewise, these factors are important for the processes that assemble and structure communities. Amphibians, besides being highly susceptible to environmental conditions, have various reproductive strategies, such as the direct development of individuals. Several hypotheses have been raised about possible selective pressures related to the emergence of direct development in anurans, as well as the relationship between environmental characteristics and the occurrence of these species. Such investigations, however, have mainly focused on specific clades and/or regions. Here, we use structural equation modelling to investigate the relationships between different abiotic (temperature, precipitation, humidity, and terrain slope) and biotic (phylogenetic composition and functional diversity) factors and the proportion of species with direct development in 766 anuran communities of the Atlantic Forest, a biome with a vast diversity of anuran species and high environmental complexity. Anuran communities with higher proportions of direct developing species were found to be mainly influenced by low potential evapotranspiration, low temperature seasonality, and high functional diversity. Phylogenetic composition and terrain slope were also found to be important in determining the occurrence of these species in Atlantic Forest communities. These results show the importance of these factors in the structuring of these communities and provide important contributions to the knowledge of direct development in anurans.

Mechanistic understanding of how temperature and its variability shape body size composition in moth assemblages

Abstract Understanding how climate affects trait composition within a biological assemblage is critical for assessing and eventually mitigating climate change impacts on the assemblage and its ecological functioning. While body size is a fundamental trait of animals as it affects many aspects of species' biology and ecology, it remains unclear through what mechanisms temperature and its variability influence within‐assemblage body size variation. This study aims to understand how temperature and its variability shape body size variations in animal assemblages and potentially affect assemblages' vulnerability to climate change. Using >5300 individuals of 680 macromoth species collected from 13 assemblages along a ca. 3000 m elevational gradient in Taiwan, we examined (1) the strength of environmental filtering and niche partitioning in determining the intra‐ and inter‐specific size variations within an assemblage, and (2) the effects of mean temperature and the daily and seasonal temperature variabilities on the strength of the two processes. We found that the body size composition was strongly affected by temperature and its seasonality via both processes. High temperature seasonality enhanced niche partitioning, causing within‐population size convergence. In contrast, low mean temperature and low seasonality both enhanced environmental filtering, causing within‐assemblage size convergence. However, while low temperature restricted the lower size limit within an assemblage, low seasonality restricted both lower and upper size limits. This study indicates an overlooked but important role of temperature seasonality in shaping intra‐ and inter‐specific size variations in moth assemblages through both environmental filtering and niche partitioning. With rising temperatures and amplifying seasonality around the globe, potentially weakened filtering forces may increase the size variation within assemblages, reinforcing the assemblage‐level resilience. Nevertheless, enhanced niche partitioning may limit size variation within populations, which may increase the population‐level vulnerability to environmental changes. This study improves the mechanistic understanding of the climatic effects on trait composition in animal assemblages and provides essential information for biodiversity conservation under climate change. Read the free Plain Language Summary for this article on the Journal blog.

Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons

Neptunea cumingii is an economically important marine shellfish found in the Yellow and Bohai Seas areas of China. In this study, samples of Neptunea cumingii were collected in Zhangzidao and Yantai during spring, summer, autumn, and winter to clarify the gene expression patterns and regulatory mechanisms in their gills in different seasons. Transcriptome analysis was conducted using Neptunea cumingii gill tissues, and genes with significantly different expression levels were extracted for functional verification. The most genes with differences in expression (DEGs) were found in comparisons of the winter and summer samples. Gene enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms showed that these DEGs were mainly involved in immune and metabolic pathways, and they had significant effects on oxidative stress, body metabolism, and protein synthesis in Neptunea cumingii. Further screening of DEGs identified thirty-four genes related to temperature regulation, comprising thirteen genes with roles in innate immunity in shellfish, twelve genes related to oxidative stress, and nine genes related to protein synthesis and energy metabolism. Eleven DEGs were randomly selected for qPCR verification, and the results were consistent with the transcriptome analysis results. In summary, the transcriptome results differed significantly between seasons in the gill tissues of Neptunea cumingii. The expression levels of immune regulatory genes could be promoted in Neptunea cumingii during the high temperature season, whereas the expression of these genes may be inhibited in the low temperature season. The results obtained in this study provide insights into the molecular defense mechanisms that might allow Neptunea cumingii to adapt to climate change.

Adding Corbicula fluminea altered the effect of plant species diversity on greenhouse gas emissions and nitrogen removal from constructed wetlands in the low-temperature season

Plant species diversity is crucial in greenhouse gas emissions and nitrogen removal from constructed wetlands (CWs). However, previous studies have overlooked the impact of benthos on cumulative greenhouse gas emissions during the low-temperature season in CWs. In this study, we established 66 vertical flow CWs with three levels of species richness (1, 2, and 4 species) and eleven species compositions. The Corbicula fluminea was added or not added at each diversity level and monitored greenhouse gas emissions and effluent nitrogen concentration. Our findings indicated that (1) in microcosms without C. fluminea, high species richness significantly increased effluent nitrogen concentrations (NO3−-N, NH4+-N, and TIN), but plant species richness did not affect cumulative CH4, N2O, and CO2 emissions. The presence of Hemerocallis fulva significantly increased cumulative CO2 emissions, while the presence of Iris tectorum significantly increased effluent nitrogen (NO3−-N and TIN) concentrations and cumulative N2O emissions; (2) in microcosms with C. fluminea, the lowest cumulative CH4 emissions occurred when there were two species, but plant species richness did not affect cumulative CO2 and N2O emissions. The presence of H. fulva significantly increased cumulative CH4 emissions, while the presence of Reineckea carnea significantly increased effluent nitrogen (NO3−-N, NH4+- N, TIN) concentrations; (3) at the same diversity level, the addition of C. fluminea significantly increased cumulative CH4 and N2O emissions, as well as effluent nitrogen concentrations. These results demonstrate that C. fluminea alters the effect of plant species diversity on cumulative greenhouse gas emissions and nitrogen removal from CWs during the low-temperature season. We recommend using a two-species mixture to reduce greenhouse gas emissions. However, we caution against using plant compositions with H. fulva or I. tectorum for effective wastewater treatment and greenhouse gas reduction in CWs.

Photothermal and Photovoltaic Utilization for Improving the Thermal Environment of Chinese Solar Greenhouses: A Review

A Chinese solar greenhouse (CSG) is an agricultural facility type with Chinese characteristics. It can effectively utilize solar energy during low-temperature seasons in alpine regions. The low construction and operation costs make it a main facility for agricultural production in the northern regions of China. It plays an extremely important role in “Chinese vegetable basket projects”. Energy is one of the important issues faced by CSGs. The better climate resources in the northern regions of China make it possible to apply solar energy as a green and sustainable energy source in the production of CSGs. Faced with the increasingly serious environmental problems of the new century, the Chinese government has made a decision and put it into practice to improve the rate and efficiency of solar energy utilization in agricultural facilities. In this paper, we summarize the research on the application of photovoltaic power generation and solar thermal technology in CSGs. The application of these advanced solar technologies has made great progress. With the further improvement of economic benefits and the establishment of relevant support policies and incentive mechanisms, the combination of CSG and solar energy technology will have certain application prospects and satisfy China’s requirements for long-term sustainable development.

Potamogeton crispus restoration increased the epiphytic microbial diversity and improved water quality in a micro-polluted urban river

Special characterization and assembly of epiphytic microbial communities remain unclear in micro-polluted water column during submersed macrophytes restoration. In this study, an in-situ enclosure area sowing with turions of Potamogeton crispus (P. crispus) was conducted in a micro-polluted urban river to investigate the characterization of P. crispus and epiphytic microbial communities and their response to water environment under different water depths. Turions completely germinated in water column with <90 cm water depth and the germination speed decreased with increasing water depth within 18 days. There were obvious differences in morphological characteristics of P. crispus between deep and shallow water layers. P. crispus restoration decreased by 12–32%, 13–36%, 9–43% and 5–36% of COD, NH4+-N, TN and TP concentration, respectively, in enclosed overlying water compared to the river (P < 0.05) during 5 months of experiment. Illumina sequencing was employed to explore the epiphytic bacterial and microeukayotic communities at water depth 25–35 cm (shallow area) and 80–90 cm (deep area). A total of 9 bacterial and 12 microeukayotic dominant phyla were obtained in eight samples. It should be noted that the algae abundances were higher in shallow area than deep area but a reverse trend was observed for methanotrophs. Null model analysis revealed that dispersal limitation and undominated process was the most important assembly process, whereas stochastic processes gained more importance in shallow area than deep one. According to cooccurrence analysis (|r| > 0.6, P < 0.05), there were more strongly correlated edges in shallow area (456 edges) than deep area (340 edges). These results highlight that submerged macrophytes restoration can increase microbial diversity and improve water quality, and provide a “summer disease cured in winter” way by using could-resistant P. crispus for water purification in micro-polluted rivers in low-temperature season.

Research on heat transfer and frost resistance performance of the closed wet cooling towers tubes

The closed wet cooling tower used as refrigeration equipment should not only consider its heat transfer effect, but also its frost resistance performance in cold winter, so as to operate safely and stably in the low temperature season, thus saving electric energy. Fluent is used to simulate the falling film flow pattern on the outer wall of the heat exchange tubes, the heat transfer performance differences of the oval tube with different nominal axis ratios (major axis/minor axis) and the traditional circular tube were compared. And also the effect of spray density on the heat transfer outside the tubes has been discussed. The type of oval tubes with better thermodynamic performance was given. Then, the ANSYS static structure module is used to analyze the elastic-plastic properties of heat exchange tubes with different materials and tube types, and the tube parameters with better frost resistance are proposed. Combining the performance of heat transfer and frost resistance, it provides a reference for the design of closed wet cooling tower tubes for refrigeration.

Seasonal differences in intestinal flora are related to rats' intestinal water metabolism.

Many studies have reported obvious seasonal differences in the intestinal flora of rats, and this stable distribution of the seasonal flora helps in maintaining the normal physiological function of the host. However, the mechanism underlying these seasonal differences in intestinal flora remains unclear. To explore the correlation among seasonal factors and intestinal water metabolism and intestinal flora, 20 Sprague Dawley (SD) rats were divided into spring, summer, autumn, and winter groups. The environment for the four seasons was simulated using the Balanced Temperature and Humidity Control system. The intestinal water metabolism was evaluated by determining the intestinal transmission function, fecal water content, water content of colonic tissue, and the colonic expression levels of AQP3, AQP4, and AQP8. The composition and relative abundance of intestinal microflora in rats in each season were assessed through 16S rDNA amplifier sequencing, and the relationship between the dominant flora and intestinal water metabolism in each season was analyzed using Spearman correlation analysis. The high temperature and humidity season could lead to an increase in intestinal water metabolism and intestinal water content in rats, whereas the low temperature and humidity season could lead to a decrease, which was closely related to the change in microflora. To explore the molecular mechanism of seasonal changes in intestinal water metabolism, the concentration of colonic 5-HT, VIP, cAMP, and PKA associated with intestinal water metabolism in rats were also examined. Seasonal changes could affect the concentration of colonic 5-HT and VIP in rats, and then regulate AQPs through cAMP/PKA pathway to affect the intestinal water metabolism. These results suggest that seasonal factors affect the level of intestinal water metabolism in rats and result in seasonal differences in intestinal flora.

Pollination ecology of the early-spring-blooming dioecious shrub Eurya japonica (Pentaphylacaceae)

While the majority of angiosperm plants have hermaphrodite flowers, where a single pollinator visit can achieve both pollen removal and deposition, 5%–6% of angiosperms, including zoophilous species, are dioecious, necessitating pollen transport from male plants to females. As such, dioecy is considered less adaptive under low-temperature conditions that tend to restrict or lower potential pollinator activity. Interestingly, however, a number of dioecious, zoophilous plants such as Eurya japonica Thunb. (Pentaphylacaceae) bloom in low-temperature seasons. This study made a quantitative assessment of flower visitors/pollinators of E. japonica blooming in early spring in central Japan. A total of 15 families in 4 orders of insects were recorded on both pistillate and staminate flowers of E. japonica, of which Diptera (Empididae and Chironomidae, in particular) and Hymenoptera were predominant regardless of site/year. Hymenopteran visitors were more active at higher temperatures, while dipterans were observed even at low temperatures. The fruiting rate and the number of seeds were greater at the site where dipteran visitors were more abundant. The present study suggests that the system of multi-taxa, Diptera-dominated pollination may play an important role in the reproductive ecology of dioecious plant species, in particular those flowering at low temperatures.

ANALYSIS OF TRANSMISSION DYNAMICS OF SARS-COV-2 UNDER SEASONAL CHANGE

In this paper, we explore whether the activity of SARS-CoV-2 was associated with seasonality. MF-DFA model is utilized to calculate multifractal strength and multifractal complexity to evaluate the change state of SARS-CoV-2 activity. We select 10 countries with serious epidemic in the world, which are distributed in different latitudes of the northern and southern hemispheres. The study utilized the time series data of daily new cases and daily new deaths recorded in these countries. We regard May to October as the “high temperature season” for countries in the northern hemisphere, November to April as the “low temperature season”, and the southern hemisphere is just the opposite. By comparing the multifractal intensity [Formula: see text] and multifractal complexity [Formula: see text] of the two time series in the two seasons, we draw a conclusion that, for both the sequence of the daily newly diagnosed persons and the daily newly increased number of deaths, in the countries of both the northern and southern hemispheres, [Formula: see text] and [Formula: see text] are weaker in the “low temperature season”. That is, in the low temperature environment, SARS-CoV-2 can survive for a long time and be more infectious. In addition, we also observe that in the northern hemisphere, Iran is at a lower latitude, and although the SARS-CoV-2 activity in the low temperature season is higher than that in the high temperature season, the difference is not significant. Therefore, the lower latitude may resist this phenomenon. However, most of the countries in the southern hemisphere are within 30° of south latitude, with low latitude, and other meteorological characteristics such as humidity in the countries in the southern hemisphere are also relatively unique. Although SARS-CoV-2 is characterized by high activity in low temperature seasons, no direct evidence related to the characteristics of latitude distribution has been found.

Effects of a dietary anionic alkali mineral complex in juvenile olive flounder (Paralichthys olivaceus) during low water temperature season

This study was conducted to investigate the effects of different supplementary levels of an anionic alkali mineral complex (AAMC) containing sodium metasilicate, potassium carbonate, sodium carbonate, sodium tetraborate, sodium chloride, sodium thiosulfate, silver nitrate and sucrose on growth, innate immunity and disease resistance of juvenile olive flounder (Paralichthys olivaceus) during the low temperature season (15.0 ± 2.6 ºC). The control diet was a commercial feed and 0.5%, 1% and 2% of AAMC were sprayed to the control diet to prepare experimental diets (Control, MC-0.5, MC-1.0, and MC-2.0). Quadruplicated groups of juvenile olive flounder (9.11 ± 0.04 g) were distributed in a flow-through system as 45 fish per tank and fed one of the experimental diets to apparent satiation twice a day (08:30 and 16:30 h) for 12 weeks. The dietary supplementation of AAMC improved the feed utilization of fish. Growth performance of fish was significantly improved by feeding MC-2.0 diet compared to control group. Fish fed MC-2.0 diet showed significantly higher blood hemoglobin level compared to the control. Gut morphology of fish was improved by dietary AAMC. Innate immunity and antioxidant capacity of fish were also significantly improved by dietary AAMC. Apparent digestibility coefficient of dry matter was significantly increased in MC-1.0 diet, while protein digestibility was significantly higher in both MC-1.0 and MC-2.0 diets. The cumulative mortalities of challenged fish fed MC-1.0 and MC-2.0 diets were significantly reduced. In conclusion, the dietary supplementation of 2% AAMC significantly improved the growth performance, innate immunity, diet digestibility and disease resistance against Edwardsiella tarda in juvenile olive flounder reared in low water temperature season.

Unearthing Modes of Climatic Adaptation in Underground Storage Organs Across Liliales.

Testing adaptive hypotheses about how continuous traits evolve in association with developmentally structured discrete traits, while accounting for the confounding influence of other, hidden, evolutionary forces, remains a challenge in evolutionary biology. For example, geophytes are herbaceous plants-with underground buds-that use underground storage organs (USOs) to survive extended periods of unfavorable conditions. Such plants have evolved multiple times independently across all major vascular plant lineages. Even within closely related lineages, however, geophytes show impressive variation in the morphological modifications and structures (i.e.,"types" of USOs) that allow them to survive underground. Despite the developmental and structural complexity of USOs, the prevailing hypothesis is that they represent convergent evolutionary "solutions" to a common ecological problem, though some recent research has drawn this conclusion into question. We extend existing phylogenetic comparative methods to test for links between the hierarchical discrete morphological traits associated with USOs and adaptation to environmental variables, using a phylogeny of 621 species in Liliales. We found that plants with different USO types do not differ in climatic niche more than expected by chance, with the exception of root morphology, where modified roots are associated with lower temperature seasonality. These findings suggest that root tubers may reflect adaptations to different climatic conditions than those represented by other types of USOs. Thus, the tissue type and developmental origin of the USO structure may influence the way it mediates ecological relationships, which draws into question the appropriateness of ascribing broad ecological patterns uniformly across geophytic taxa. This work provides a new framework for testing adaptive hypotheses and for linking ecological patterns across morphologically varying taxa while accounting for developmental (non-independent) relationships in morphological data. [Climatic niche evolution; geophytes; imperfect correspondence; macroevolution.].

New insight for purifying polluted river water using the combination of large-scale rotating biological contactors and integrated constructed wetlands in the cold season

Ecological treatment technologies, applied to deal with polluted river water in the low temperature season, remain limited. In this study, a new insight was put forward for purifying polluted river water using a combination system (CS) of large-scale rotating biological contactors (RBCs) and integrated constructed wetlands in autumn and winter. The treatment performance, average removal contribution (RC), nitrification and denitrification rates, microbial community structure, and ecosystem service value were considered to estimate the combination system. Results revealed that the average removal efficiencies of ammonium (NH4+–N), total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) reached 93.9%, 20.8%, 36.5%, and 37.1%, respectively. The combination system showed excellent removal efficiency of NH4+–N regardless of the effect of low temperature. The maximum values of nitrification and denitrification rates were 59.57 g N/(m3·d) and 0.78 g N/(m2·d), respectively. Considerable differences in bacterial community diversity, richness and relative abundance of functional microbes were observed in the main treatment units, resulting in different average RC to pollutants. The unit capital cost of CS purifying polluted river water was 260 USD/m3 and the operation and maintenance cost was 0.144 million USD/yr. Meanwhile, the ecosystem service value of the CS was 0.334 million USD in autumn and winter. CS not only possessed excellent pollutant purifying efficiencies, but also achieved high ecological service value in the cold season.

Latitudinal patterns and environmental drivers of taxonomic, functional, and phylogenetic diversity of woody plants in western Amazonian terra firme forests.

Elucidating how environmental factors drive plant species distributions and how they affect latitudinal diversity gradients, remain essential questions in ecology and biogeography. In this study we aimed: 1) to investigate the relationships between all three diversity attributes, i.e., taxonomic diversity (TD), functional diversity (FD), and phylogenetic diversity (PD); 2) to quantify the latitudinal variation in these diversity attributes in western Amazonian terra firme forests; and 3) to understand how climatic and edaphic drivers contribute to explaining diversity patterns. We inventoried ca. 15,000 individuals from ca. 1,250 species, and obtained functional trait records for ca. 5,000 woody plant individuals in 50 plots of 0.1 ha located in five terra firme forest sites spread over a latitudinal gradient of 1200 km covering ca. 10°C in latitude in western Amazonia. We calculated all three diversity attributes using Hill numbers: q = 0 (richness), q = 1 (richness weighted by relative abundance), and q = 2 (richness weighted by dominance). Generalized linear mixed models were constructed for each diversity attribute to test the effects of different uncorrelated environmental predictors comprising the temperature seasonality, annual precipitation, soil pH and soil bulk density, as well as accounting for the effect of spatial autocorrelation, i.e., plots aggregated within sites. We confirmed that TD (q = 0, q = 1, and q = 2), FD (q = 0, q = 1, and q = 2), and PD (q = 0) increased monotonically towards the Equator following the latitudinal diversity gradient. The importance of rare species could explain the lack of a pattern for PD (q = 1 and q = 2). Temperature seasonality, which was highly correlated with latitude, and annual precipitation were the main environmental drivers of variations in TD, FD, and PD. All three diversity attributes increased with lower temperature seasonality, higher annual precipitation, and lower soil pH. We confirmed the existence of latitudinal diversity gradients for TD, FD, and PD in hyperdiverse Amazonian terra firme forests. Our results agree well with the predictions of the environmental filtering principle and the favourability hypothesis, even acting in a 10°C latitudinal range within tropical climates.

Residue Behavior of Methoxyfenozide and Pymetrozine in Chinese Cabbage and Their Health Risk Assessment.

Methoxyfenozide and pymetrozine are used for pest control in the cultivation of Chinese cabbage. This has raised concerns in recent years due to health risks. Therefore, this study aimed to determine the residual concentrations of pesticides in the target crop and associated health risks. The dynamics and influence of environmental factors on the dissipation of methoxyfenozide and pymetrozine residues in Chinese cabbage were investigated. Analyses were performed using a modified QuEchERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) and an optimized high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The observed half-lives of methoxyfenozide and pymetrozine in cabbage samples ranged between two sampling seasons: in May–June, half-lives of methoxyfenozide and pymetrozine were 1.20 days and 1.89 days, respectively; during October–November, half-lives of methoxyfenozide and pymetrozine were 11.8 and 2.80 days, respectively. Meanwhile, a negative Spearman correlation was found between the residual concentrations and temperature (p < 0.01). This indicates that higher temperatures resulted in higher dissipation rates for methoxyfenozide and pymetrozine, suggesting that these pesticides degraded faster at higher temperatures. Additionally, higher pesticide residues in Chinese cabbage during low-temperature seasons resulted in higher risk quotients (RQ) (RQ > 1) for both analyzed compounds, which suggests that the effect of temperature on pesticide degradation needs to be considered as an essential factor while setting up the maximum residue limits (MRL).

Structural and functional leaf diversity lead to variability in photosynthetic capacity across a range of Juglans regia genotypes.

Similar to other cropping systems, few walnut cultivars are used as scion in commercial production. Germplasm collections can be used to diversify cultivar options and hold potential for improving crop productivity, disease resistance and stress tolerance. In this study, we explored the anatomical and biochemical bases of photosynthetic capacity and response to water stress in 11 Juglans regia accessions in the U.S. department of agriculture, agricultural research service (USDA‐ARS) National Clonal Germplasm. Net assimilation rate (A n) differed significantly among accessions and was greater in lower latitudes coincident with higher stomatal and mesophyll conductances, leaf thickness, mesophyll porosity, gas‐phase diffusion, leaf nitrogen and lower leaf mass and stomatal density. High CO2‐saturated assimilation rates led to increases in A n under diffusional and biochemical limitations. Greater A n was found in lower‐latitude accessions native to climates with more frost‐free days, greater precipitation seasonality and lower temperature seasonality. As expected, water stress consistently impaired photosynthesis with the highest % reductions in lower‐latitude accessions (A3, A5 and A9), which had the highest A n under well‐watered conditions. However, A n for A3 and A5 remained among the highest under dehydration. J. regia accessions, which have leaf structural traits and biochemistry that enhance photosynthesis, could be used as commercial scions or breeding parents to enhance productivity.

Disentangling the Mechanisms Shaping the Prokaryotic Communities in a Eutrophic Bay.

ABSTRACTEutrophication occurring in coastal bays is prominent in impacting local ecosystem structure and functioning. To understand how coastal bay ecosystem function responds to eutrophication, comprehending the ecological processes associated with microbial community assembly is critical. However, quantifying the contribution of ecological processes to the assembly of prokaryotic communities is still limited in eutrophic waters. Moreover, the influence of these ecological processes on microbial interactions is poorly understood. Here, we examined the assembly processes and co-occurrence patterns of prokaryotic communities in a eutrophic bay using 156 surface seawater samples collected over 12 months. The variation of prokaryotic community compositions (PCCs) could be mainly explained by environmental factors, of which temperature was the most important. Under high environmental heterogeneity conditions in low-temperature seasons, heterogeneous selection was the major assembly process, resulting in high β-diversity and more tightly connected co-occurrence networks. When environmental heterogeneity decreased in high-temperature seasons, drift took over, leading to decline in β-diversity and network associations. Microeukaryotes were found to be important biological factors affecting PCCs. Our results first disentangled the contribution of drift and microbial interactions to the large unexplained variation of prokaryotic communities in eutrophic waters. Furthermore, a new conceptual model linking microbial interactions to ecological processes was proposed under different environmental heterogeneity. Overall, our study sheds new light on the relationship between assembly processes and co-occurrence of prokaryotic communities in eutrophic waters.IMPORTANCE A growing number of studies have examined roles of microbial community assembly in modulating community composition. However, the relationships between community assembly and microbial interactions are not fully understood and rarely tested, especially in eutrophic waters. In this study, we built a conceptual model that links seasonal microbial interactions to ecological processes, which has not been reported before. The model showed that heterogeneous selection plays an important role in driving community assembly during low-temperature seasons, resulting in higher β-diversity and more tightly connected networks. In contrast, drift became a dominant force during high-temperature seasons, leading to declines in the β-diversity and network associations. This model could function as a new framework to predict how prokaryotic communities respond to intensified eutrophication induced by climate change in coastal environment.