Low Stress Levels Research Articles

Factors influencing children's emotional and behavioral problems perceived by North Korean refugee mothers: a descriptive study.

The study investigated the relationships between key variables, including the level of parenting knowledge, acculturative stress, depression, and the strengths and difficulties associated with their children's emotions and behavior, among North Korean refugee mothers who faced the challenging task of adapting to Korean society while raising their children. A descriptive study design was used. Data were collected from 130 North Korean refugee mothers between September and November 2023. The participants had very limited parenting knowledge, and 82.3% of them scored 21 or higher on the depression scale used in community epidemiological studies. Higher levels of parenting knowledge and lower levels of depression were associated with lower levels of acculturative stress. Moreover, higher levels of depression were associated with more emotional and behavioral difficulties in children. Maternal depression, age, and employment status were identified as factors that influenced emotional and behavioral problems in preschool-aged children. These findings underscore the importance of providing parent education and mental health support programs for North Korean refugee families in order to improve parents' parenting skills and emotional well-being.

Shear strength enhancement at the sand-steel interface: A pioneering approach with Polyurethane Foam Adhesive (PFA)

This study explores a novel application of Polyurethane Foam Adhesive (PFA) injection to augment shear resistance at the interface, investigating various parameters such as PFA content, injection pressure, curing time, sand density, and surface roughness. Results demonstrate that injecting 10 % PFA increased the friction angle (ϕ) from 6.4 to 27.4° and 32.6–42° for sand-smooth and rough surface interfaces, respectively, under relative density (Dr) of 30 %. Correspondingly, cohesion (c) values rose from 0 to 124 and 62 kPa. Samples with lower Dr (30 %) exhibited a more significant increase in shear strength post-PFA injection, attributed to PFA penetration and enhanced shear performance of loose sands, showing a maximum 23 % difference compared to Dr = 90 % for the sand-smooth surface interface. Curing time notably influenced injected samples, particularly at lower normal stress levels, leading to increased mobilized shear strength with longer curing periods, with 28-day shear strength averaging 2.34 and 2.74 times higher than one-hour shear strength for smooth and rough surfaces, respectively. A substantial shear strength increase was observed one hour after injection, with more pronounced behavior after seven days. Additionally, increased injection pressure expanded the stabilized area at the interface, enhancing mobilized shear strength. At the sand-smooth surface interface, increasing injection pressure from 25 to 225 kPa resulted in average mobilized shear strength increases of 84% and 117% for Dr 30 % and 90 %, respectively. Examination under a Scanning Electron Microscope (SEM) revealed that the inclusion of PFA additive serves to interconnect adjacent grains, consequently enhancing the mechanical properties of the mixture.

Stress and academic engagement among Saudi undergraduate nursing students: The mediating role of emotion regulation and emotional intelligence.

To assess the mediating role of emotion regulation and emotional intelligence in the relationship between stress and academic engagement among Saudi undergraduate nursing students. This study employed a quantitative cross-sectional research design. The study recruited 367 Saudi undergraduate nursing students at a major Saudi university. Structural equation modelling was used to explore the mediational model. The results of the ANOVA and Welch F-test demonstrated that the emotional intelligence, emotional regulation and academic engagement scores were statistically significantly different according to stress levels (p-values <0.01). Perceived stress has a statistically significant moderate negative correlation with academic engagement and emotional intelligence and a strong negative correlation with emotional regulation. The results also showed that academic engagement had a statistically significant moderate positive association with emotional intelligence and emotional regulation. Results indicate that stress and academic engagement are negatively correlated among Saudi undergraduate nursing students. It focuses on the balancing functions of emotional regulation and emotional intelligence, highlighting their ability to lower stress levels and improve academic engagement. No Patient or Public Contribution.

Affect variability and cortisol in context: The moderating roles of mean affect and stress

Positive and negative affect have been shown to have implications for hormones like cortisol but how moment to moment changes in affect (i.e., affect variability) influence cortisol secretion is less well understood. Additionally, context characteristics such as mean affect and stress may influence the association between affect variability and cortisol output. In the current study, we examined affect, stress, and cortisol data from 113 participants (age range = 25–63, M = 35.63, SD = 11.34; 29% male; 42% White/Caucasian, 37% Asian or Pacific Islander, 13% Hispanic/Latino, 4% Black/African American, 1% Native American, Eskimo, or Aleut, 4% selected “other” for their race/ethnicity). Participants completed ecological momentary assessments assessing positive and negative affect and stress four times per day for five days and provided saliva samples at each time point. Saliva was assayed for cortisol, and area under the curve with respect to ground was computed. In a three-way interaction, both positive affect mean level and stress moderated the association between positive affect variability and cortisol (b = −1.55, t(100) = −3.29, SE = 0.47, p <.01, β = −4.05). When breaking down this three-way interaction, in the context of low stress and high mean positive affect, variability was positively related to total cortisol output. In contrast, in the context of high stress and high mean positive affect, variability was negatively related to total cortisol output. While greater positive affect variability is generally worse for health-relevant outcomes (as prior research has shown and as we show here at low levels of stress), at high levels of stress, fluctuation in affect may be adaptive. For someone experiencing a high stress week, having fluctuations in positive affect may mean that they are adaptively changing to meet their environmental needs especially when they typically report high mean positive affect levels. There were no associations between negative affect variability and cortisol secretion nor did mean negative affect or stress play a moderating role for negative affect variability. This study provides evidence that positive affect variability’s association with cortisol secretion throughout the day may vary based on stress and mean positive affect levels.

Professional quality of life and organizational support: a survey of physical therapists with experience as clinical instructors

Purpose: The purpose of this study was to determine the professional quality of life (ProQoL) of physical therapists (PTs) with experience as a clinical instructor and identify factors associated with compassion satisfaction, burnout, and secondary traumatic stress. Methods: This study used a cross-sectional design and survey methodology. Participants were clinician PTs who have been clinical instructors, and completed an online survey that included the ProQoL scale, the eight-item Survey of Perceived Organizational Support, and demographics. Results: Of the 259 participants who completed the survey, the majority experienced average to high levels of compassion satisfaction and low levels of burnout and secondary traumatic stress. Chi-square tests of independence indicated high ratings of compassion satisfaction were associated with the uppermost years licensed as a PT, years of clinical teaching experience, and age. Participants aged 26–35 or with less than 5 years of clinical instruction experience correlated with low ratings of compassion satisfaction. Participants with more than 25 years of experience as PTs correlated with low ratings of burnout. High levels of compassion satisfaction were associated with high levels of perceived organizational support. Conclusion: Findings may assist clinical sites and site coordinators of clinical education in selecting and training clinical instructors.

STRESSORS AND COPING MEASURES AMONG UNDERGRADUATE NURSING STUDENTS IN WESTERN NIGERIA

Nursing students are frequently exposed to various stressors throughout their training; the resultant stress affects their personal, professional, and academic achievements and well-being directly or indirectly. This study aimed to identify the causes/sources of stress, the levels of stress, coping measures, and possible suggestions for reducing stress and its effects among nursing students. A descriptive cross-sectional design was used to assess stress and coping variables among undergraduate nursing students at a federal university in Western Nigeria. This study recruited 151 full-time undergraduate nursing students in their first to fifth study years using a simple random sampling technique. Data were collected using the Perceived Stress Scale (PSS) and self-structured questions. The results were summarized using descriptive statistics of frequency, percentages, and mean ± SD. Academic, personal, financial, and relationship-related stressors were found to be the most common sources of stress. Most of nursing students experienced either low (47.7%) or severe (44.3%) stress levels and coped with it through positive thinking, optimism, sleep, and relaxation. Respondents suggested that more emphasis be placed on health promotion programs, reducing the number of exams in a week, and teaching students how to develop their social, coping, and time management skills.

Study on Corrosion Fatigue Behavior of 304L Austenite Stainless Steel in 325 °C High-Temperature Water Environment

The fatigue crack growth behavior of 304L austenitic stainless steel (SS) in a 325 °C high-temperature and high-pressure water environment were investigated by a corrosion fatigue test system, by electron back scatter diffraction (EBSD), and by a transmission electron microscope (TEM). The experimental results indicated that the crack growth rate (CGR) of 304L SS increases with increasing the stress intensity factor, stress level, and fatigue frequency (f). Compared to dissolved hydrogen (DH) in a high-temperature water environment, dissolved oxygen (DO) significantly enhances the CGR by about an order of magnitude higher. The crack tip of 304L SS after the corrosion fatigue test under higher stress levels is sharper, with more secondary cracks on the fracture surface, while the crack tip under lower stress levels is blunter with relatively fewer secondary cracks. The oxidation behavior at the crack tip was analyzed under different loading and water chemistry conditions, and a related effect on the crack tip and CGR was clarified.

The Impact of Relocation Patterns on Psychological Stress

This study investigated how relocation patterns affect disaster survivors’ psychological stress on the diverse durations and spaces of relocation. It analyzed a 10-year data set of 1,236 families affected by 2009’s Typhoon Morakot in Taiwan, identifying six relocation patterns through dynamic time warping (DTW). A hierarchical linear model was utilized, revealing the discernible impacts of environmental factors, sociocultural factors, and family-level socioeconomic factors on psychological stress. The study revealed that survivors who quickly found stable residences after the disaster initially experienced lower stress levels, but in the long term, their stress increased. Conversely, those with unstable residences experienced higher initial stress but lower long-term stress. Comparing similar patterns, we found that survivors who had more time for preparation and who sought opportunities, coped, or adapted to secondary stressors before long-distance relocation faced lower stress levels. These findings suggest that relocation patterns have a greater impact on the psychosocial stress of disaster survivors than time or relocation distance.

A hypoplastic model for pre- and post-liquefaction analysis of sands

This article proposes an extended constitutive model for liquefaction analysis of sands. The proposed model is formulated based on the hypoplastic model for granular soils by von Wolffersdorf (1996), enhanced with Intergranular Strain Anisotropy by Fuentes et al. (2019), which is usually referred to as ISA-hypoplasticity. The proposed extended model includes some modifications that are indispensable for the correct prediction of liquefaction-related analysis: (i) a new Lode-angle-dependent function for post-liquefaction shear strain accumulation, (ii) a modification for fabric change effects, (iii) the theory of the so-called semifluidized state. The first modification allows the model to predict shear strain accumulation in extension and compression during cyclic mobility. The second and third modifications, were initially developed by Liao et al. (2022) for hypoplasticity with conventional intergranular strain based on the pioneer work by Barrero et al. (2020) for Sanisand, and enable the model to reproduce fabric change effects upon loading reversal, and stiffness and dilatancy degradation at low effective stress levels, respectively. With the consideration of the new Lode-angle function, the proposed model realistically predict the increasing shear strain accumulation in both extension and compression without adopting a circular critical state surface, as adopted in Liao et al. (2022). The proposed model was carefully calibrated and validated based on a series of monotonic and cyclic triaxial tests on Zbraslav and Karlsruhe fine sands, considering various testing conditions. The comparison between experimental measurements and numerical predictions of undrained cyclic tests suggests that the proposed model accurately describes the pre- and post-liquefaction stages, as well as the stress attractors.

Music-making facilitates acculturation and reduces acculturative stress: Evidence from a survey of migrants living in Germany

Beneficial effects of music on wellbeing and group cohesion are well established. Furthermore, participation in music groups has been shown to be associated with increased orientation to the host culture, while orientation to culture of origin appears to remain unaffected. In order to gain insight into the effects of music activities on acculturative stress in adult migrants, a group of musically active migrants to Germany ( n = 80) was compared with migrants who had never played a musical instrument ( n = 86). We saw that music group members ( n = 42) reported lower levels of acculturative stress and a stronger orientation to mainstream culture compared to participants who are not actively making music. The association between music group membership and acculturative stress was mediated by orientation to host culture. Solo-musicians ( n = 38) did not differ from group-musicians and musically non-active subjects. We found no differences in orientation to the culture of origin between the study groups. The findings suggest that (group) music making can support acculturation processes in migrants.

The Effect of Internal Gas Pressure on the Compression Properties of Natural Rubber Foams.

This study explores the effect of internal gas pressure (P) on closed-cell natural rubber (NR) foams. Three key factors are analyzed using a 3D model during uniaxial compression: (1) the initial gas pressure (P0 = 1, 2, and 3 atm) inside the cells, (2) different cell sizes (D = 0.1, 0.2, 0.3, and 0.4 mm in diameter), and (3) the presence of defects (holes in the cell walls) in terms of their sizes (d = 0.07 to 0.1 mm). The findings reveal a negative relationship between the initial gas pressure and the relative internal gas pressure (α = P/P0) and a direct correlation with stress during compression. For instance, a change from 1 to 3 atm of the initial internal gas pressure results in a 158% decrease in α with only a 3% increase in stress. Larger cell sizes contribute to higher α but lower stress levels during compression. Changing the cell size from 0.1 to 0.4 mm generates a 27% increase in α but a 45% drop in stress. An analysis of hole sizes (cell connection) indicates that larger holes result in higher relative internal gas pressure, while smaller holes lead to higher stress levels because of more flow restriction. For example, increasing the hole size from 0.07 to 0.1 mm leads to an 8% higher α but a 32% stress reduction. These findings highlight the significant effect of the internal gas pressure inside the cells in determining the mechanical properties of rubber foams, which are generally neglected. The results also provide useful insights for better material design and different industrial applications. This study also generates predictive models to understand the relationships between stress, strain, initial gas pressure, cell size, and defects (holes/connections), enabling the production of tailor-made rubber foams by controlling their mechanical behavior.

Effect of novel polyethylene insert configurations on bone-implant micromotion and contact stresses in total ankle replacement prostheses: a finite element analysis.

This study investigates the impact of elastic improvements to the artificial ankle joint insert on prosthesis biomechanics to reduce the risk of prosthesis loosening in TAR patients. CT data of the right ankle was collected from one elderly female volunteer. An original TAR model (Model A) was developed from CT images and the INBONE II implant system. The development of the new inserts adopts an elastic improvement design approach, where different geometric configurations of flexible layers are inserted into the traditional insert. The structure can be divided into continuous flexible layers and intermittent flexible layers. The flexible layers aim to improve the elasticity of the component by absorbing and dispersing more kinetic energy. The newly designed inserts are used to replace the original insert in Model A, resulting in the development of Models B-D. A finite element model of gait analysis was based by gait parameters. Discrepancies in micromotion and contact behaviour were analysed during the gait cycle, along with interface fretting and articular surface stress at 50% of the gait cycle. In terms of micromotion, the improved elastic models showed reduced micromotion at the tibial-implant interfaces compared to the original model. The peak average micromotion decreased by 12.1%, 13.1%, and 14.5% in Models B, C, and D, respectively. The micromotion distribution also improved in the improved models, especially in Model D. Regarding contact areas, all models showed increased contact areas of articular surfaces with axial load, with Models B, C, and D increasing by 26.8%, 23.9%, and 24.4%, respectively. Contact stress on articular surfaces increased with axial load, reaching peak stress during the late stance phase. Models with continuous flexible layer designs exhibited lower stress levels. The insert and the talar prosthetic articular surfaces showed more uniform stress distribution in the improved models. Improving the elasticity of the insert can enhance component flexibility, absorb impact forces, reduce micromotion, and improve contact behavior. The design scheme of continuous flexible layers is more advantageous in transmitting and dispersing stress, providing reference value for insert improvement.

Micro-mechanics investigation of heterogeneous deformation fields and crack initiation driven by the local stored energy density in austenitic stainless steel welded joints

This work investigates the heterogeneous deformation and failure of HR3C austenitic stainless steel welded joints at room and typical service temperatures. Such types of welded joints are widely used in the new generation of fossil fuel power stations and are known to suffer from premature high temperature failure. Observation of in-service failures revealed that cracks may nucleate either in the heat affected zone or in the weld metal. The main objective of this work is to identify the local microstructural conditions and stress–strain fields responsible for both ductile failure and micro-crack nucleation within the weldment at low and high temperatures, respectively. To that purpose, a novel multi-scale micromechanics-based modelling framework is proposed. It relies on representative weld microstructure models digitally reconstructed from electron backscatter diffraction measurements, and dislocation density-based crystal plasticity models to describe the behaviour of individual grains in each weld region. A novel thermo-dynamically consistent relation for the configuration stored energy per unit volume is derived from the crystal plasticity framework.The single crystal models are calibrated and validated from a combination of uniaxial tensile tests on cross-weld specimens using high resolution digital image correlation techniques, representative volume elements of the polycrystals at the macro-scale, as well as micropillar compression tests at the scale of the individual grains. Predictions of heterogeneous inelastic deformation fields within the weldment at both 22 °C and 665 °C, and of micropillar compression behaviour of the individual weld single crystal phases are consistent with experimental data. The experimentally observed ductile failure of the weld metal at room temperature is successfully predicted using a Gurson-type void nucleation, growth and coalescence constitutive model. The suitability of the stored strain energy density and the accumulated plastic strain as crack initiation/creep failure indicators is investigated. It was found that predicted creep lifetimes with either indicator were very similar, and that they were relatively accurate for low stress levels.

NOX2 and NOX4 expression in monocytes and macrophages-extracellular vesicles in signalling and therapeutics.

Extracellular vesicles (EVs) are a type of cytoplasmic vesicles secreted by a variety of cells. EVs originating from cells have been known to participate in cell communication, antigen presentation, immune cell activation, tolerance induction, etc. These EVs can also carry the active form of Nicotinamide Adenine Dinucleotide Phosphate Oxidase Hydrogen (NADPH) oxidase, which is very essential for the production of reactive oxygen species (ROS) and that can then modulate processes such as cell regeneration. The aim of this study is to characterize the EVs isolated from U-937 and THP-1 cells, identify the NADPH oxidase (NOX) isoforms, and to determine whether EVs can modulate NOX4 and NOX2 in monocytes and macrophages. In our study, isolated EVs of U-937 were characterized using dynamic light scattering (DLS) spectroscopy and immunoblotting. The results showed that the exogenous addition of differentiation agents (either phorbol 12-myristate 13-acetate (PMA) or ascorbic acid) or the supplementation of EVs used in the study did not cause any stress leading to alterations in cell proliferation and viability. In cells co-cultured with EVs for 72h, strong suppression of NOX4 and NOX2 is evident when monocytes transform into macrophagic cells. We also observed lower levels of oxidative stress measured using immunoblotting and electron paramagnetic resonance spectroscopy under the EVs co-cultured condition, which also indicates that EVs might contribute significantly by acting as an antioxidant source, which agrees with previous studies that hypothesized the role of EVs in therapeutics. Therefore, our results provide evidence for NOX regulation by EVs in addition to its role as an antioxidant cargo.

Development and application of a learning enjoyment scale for pedagogical activities

The impact of learning enjoyment on motivation, enthusiasm, and overall learning experiences is significant. Previous studies, lacking an unbiased tool for measuring enjoyment and confronting various influencing factors, produced conflicting results regarding enjoyment levels in different instructional methods. Hence, we developed a learning enjoyment scale for evaluating both active and passive educational activities. We applied the developed scale to 112 first-year medical and dental students to assess their enjoyment during didactic physiology lectures and explored possible associated factors. Within this data note, we present students’ responses to the developed LES. The LES encompasses six dimensions: knowledge, comprehension, application, analysis, concentration, and enjoyment. Students provided ratings for each dimension on a five-point Likert scale, spanning from 1 (strongly disagree) to 5 (strongly agree). The cumulative scores across the six dimensions range from a minimum of 6 to a maximum of 30. These total scores can be categorized as excellent (&gt; 24), acceptable (18-24), or low (&lt; 18). The second section of the dataset examines specific factors influencing overall enjoyment, such as teacher proficiency, topic difficulty, active student participation, objectives fulfillment, low stress levels, and self-perceived acquisition of skills. In addition to objective measurement of students’ enjoyment level, the LES can be utilized for quantitative cross-comparisons between different teaching activities. By employing this dataset, we will undertake an analysis to determine the internal consistency of the Learning Enjoyment Scale (LES), with the anticipation that the outcomes will be published in another venue.

Promoting stress and anxiety recovery in older adults: assessing the therapeutic influence of biophilic green walls and outdoor view.

Previous research has already provided evidence regarding the favorable impact of green walls and outdoor views on stress reduction and anxiety alleviation. However, there has been limited exploration into the combined effects of green walls and outdoor views on older adults. In this study, a between-subjects experiment was conducted using virtual reality (VR) technology with 23 participants. Following exposure to stressors, each participant underwent four randomized sessions, each lasting 5 min, in various virtual reality (VR) environments, encompassing non-biophilic and biophilic environments (including green walls, outdoor views, and their combination). Throughout the process, we measured physiological indicators of stress responses, including heart rate, heart rate variability, skin conductance levels, and blood pressure, using biometric sensors. Psychological changes in participants, including anxiety levels, were evaluated through the State-Trait Anxiety Inventory, recovery scales, and self-reported emotional assessments. In conclusion, in comparison to non-biophilic environments, older adults consistently exhibited lower stress levels, experienced superior anxiety relief, and demonstrated improved recovery in nature connectedness environments, with a notably faster recovery rate. These findings suggest that the incorporation of nature connectedness principles into the indoor environments of public activity spaces within older adults care facilities can significantly contribute to stress reduction and anxiety alleviation among older adults. Furthermore, these effects appear to be contingent on the specific types of nature connectedness environments. These results can provide substantial evidence to support the design of indoor common activity spaces within older adults care facilities.

Cognitive, emotional, and social factors promoting psychosocial adaptation: a study of latent profiles in people living in socially vulnerable contexts.

Social adaptation is a multifaceted process that encompasses cognitive, social, and affective factors. Previous research often focused on isolated variables, overlooking their interactions, especially in challenging environments. Our study addresses this by investigating how cognitive (working memory, verbal intelligence, self-regulation), social (affective empathy, family networks, loneliness), and psychological (locus of control, self-esteem, perceived stress) factors interact to influence social adaptation. We analyzed data from 254 adults (55% female) aged 18 to 46 in economically vulnerable households in Santiago, Chile. We used Latent profile analysis (LPA) and machine learning to uncover distinct patters of socioadaptive features and identify the most discriminating features. LPA showed two distinct psychosocial adaptation profiles: one characterized by effective psychosocial adaptation and another by poor psychosocial adaptation. The adaptive profile featured individuals with strong emotional, cognitive, and behavioral self-regulation, an internal locus of control, high self-esteem, lower stress levels, reduced affective empathy, robust family support, and decreased loneliness. Conversely, the poorly adapted profile exhibited the opposite traits. Machine learning pinpointed six key differentiating factors in various adaptation pathways within the same vulnerable context: high self-esteem, cognitive and behavioral self-regulation, low stress levels, higher education, and increased social support. This research carries significant policy implications, highlighting the need to reinforce protective factors and psychological resources, such as self-esteem, self-regulation, and education, to foster effective adaptation in adversity. Additionally, we identified critical risk factors impacting social adaptation in vulnerable populations, advancing our understanding of this intricate phenomenon.

Occupational Stress Related to Workplace Violence among Emergency Medical Workers Working in Emergency Medical Centers in Alborz Province

Background and Objectives Health system workers, especially those like emergency medical personnel who are in direct contact with patients, are highly susceptible to workplace violence. A significant consequence of such violence is occupational stress. This study aims to explore the prevalence of workplace violence and its association with occupational stress among emergency medical workers in Alborz province in the year 2022. Subjects and Methods This descriptive correlational study was conducted with 124 emergency medical workers at road and urban bases in Alborz province. The research tools included a Persian-adapted standard workplace violence questionnaire and the occupational stress questionnaire from the Health and Safety Institute of England. Data were analyzed using descriptive and inferential statistics via SPSS software, version 26. Results The findings revealed that 62.1% of participants experienced physical violence, and 90% faced psychological violence. The highest incidence of physical (47.4%) and psychological (41.4%) violence occurred during the evening shift, while the morning shift saw the lowest rates. The average occupational stress levels for workers at road and urban bases were significantly high, measured at 120.5 ± 11.49 and 110.8 ± 16.76, respectively. Occupational stress was found to correlate negatively with the age and work experience of the employees, indicating lower stress levels with increasing age and experience. Conclusion Emergency medical workers often face highly stressful and anxious situations involving patients and their families. The nature of their work frequently exposes them to violence from patients or their companions, leading to heightened occupational stress.

Influence of Vertical Straightener Roller Shape on Residual Stress Level in Railway Rails

The required rail straightness is achieved by straightening with roller straighteners. The consequence of the straightening operation is the introduction of residual stresses to the straightened rail. An excessive level of residual stresses accumulated in the rail during use in the track may lead to its damage or fracture. ArcelorMittal Poland S.A., in cooperation with Łukasiewicz Research Network – Institute for Ferrous Metallurgy, carried out a research project (POI R.01.02.00-00-0167/16) the aim of which was to reduce residual stresses in railway rails by changing the technological parameters of the straightening process. The results of the presented study relate to rails 60E1 and 60E2. The study includes the measurement, testing, calculations and analyses of the obtained results. The conducted research indicates the possibility of obtaining a low level of residual stress in the rails for a system consisting of a 7-roller vertical straightener and a 9-roller horizontal straightener by changing the roller settings, the shape of the rollers, the shape of the rail foot and its curvature.

Celastrol enhances the viability of random-pattern skin flaps by regulating autophagy through the AMPK-mTOR-TFEB axis.

In reconstructive and plastic surgery, random-pattern skin flaps (RPSF) are often used to correct defects. However, their clinical usefulness is limited due to their susceptibility to necrosis, especially on the distal side of the RPSF. This study validates the protective effect of celastrol (CEL) on flap viability and explores in terms of underlying mechanisms of action. The viability of different groups of RPSF was evaluated by survival zone analysis, laser doppler blood flow, and histological analysis. The effects of CEL on flap angiogenesis, apoptosis, oxidative stress, and autophagy were evaluated by Western blot, immunohistochemistry, and immunofluorescence assays. Finally, its mechanistic aspects were explored by autophagy inhibitor and Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) inhibitor. On the seventh day after surgery, the survival area size, blood supply, and microvessel count of RPSF were augmented following the administration of CEL. Additionally, CEL stimulated angiogenesis, suppressed apoptosis, and lowered oxidative stress levels immediately after elevated autophagy in ischemic regions; These effects can be reversed using the autophagy inhibitor chloroquine (CQ). Specifically, CQ has been observed to counteract the protective impact of CEL on the RPSF. Moreover, it has also been discovered that CEL triggers the AMPK-mTOR-TFEB axis activation in the area affected by ischemia. In CEL-treated skin flaps, AMPK inhibitors were demonstrated to suppress the AMPK-mTOR-TFEB axis and reduce autophagy levels. This investigation suggests that CEL benefits the survival of RPSF by augmenting angiogenesis and impeding oxidative stress and apoptosis. The results are credited to increased autophagy, made possible by the AMPK-mTOR-TFEB axis activation.