The loess-paleosol sequence of the Striguny outcrop is located in the Belgorod region, in the forest-steppe area. The study of the depositional environment, pedogenesis, and pedostratigraphy of the MIS5 paleosols within the OSL chronological framework is the focus of this paper. Field data are supported by micromorphology, grain size distribution, chemical, and geochemical properties. The buried paleosols reveal the dramatic environmental trend (progressive aridization and cooling) from the Last Interglacial to the end of the Early Glacial. The lower paleosol (Albic Retisol) corresponds to the moderately warm and humid climate of boreal mixed forests during the Last Interglacial (MIS5e). Throughout the transition to the Early Glacial, boreal forests were replaced by productive boreal steppe with Chernozems and then by cold, arid steppe with Calcic Cambisols. Cryogenesis accompanied pedogenesis during the whole Early Glacial period. The diagenetic impact resulted in a decrease in organic carbon and the calcification of Albic Retisol. The tripartite pattern of the MIS5 chronozone of LPS Striguny may serve as a good stratigraphic marker for the onset of the Late Pleistocene, allowing correlation with loess-paleosol sequences of the Northern Hemisphere and linking it with global climatic fluctuations recorded in marine sediments. The buried Glossic Albic Retisol is similar to the modern soils of the Southern Taiga, which now occur 500 km to the north of the study area. This means that the bioclimatic pattern during the last interglacial was different than it is today. The present study establishes the current most southerly position of the forest soil of the Last Interglacial on the Russian Plain.