Organic matter enrichment in lacustrine shale is significantly influenced by paleoclimate and paleoenvironment. In this study, a total of 24 shale samples were vertically collected from the YQ-1 drilling well in the northern Qaidam Basin, north-western China. The high-resolution geochemistry analyses were performed to provide a detailed geological description of these samples through lithofacies interpretation, total organic carbon (TOC) content testing, element geochemistry analysis, and mineralogical composition characterization. The results show that four lithofacies types can be identified in the Middle Jurassic Shimengou Formation, which consist of the brown oil shale with horizontal bedding, the black shale with well-developed lamination, the grey-black mudstone with horizontal and wavy bedding, and the carbonaceous mudstone with plant debris. The former two types were preserved in the Upper Member and were mainly deposited in a deep and semi-deep lake environment, whereas the latter two types were preserved in the Lower Member and were deposited in a littoral-shallow lake. The TOC content, paleo-salinity, redox action, and paleo-productivity of the shales in the Upper Member are higher than those in the Lower Member, and other parameters, such as the chemical weathering degree and terrestrial clastic input, are higher in the shales of the Lower Member. Based on lithofacies and geochemical data, the shales in the Lower Member were deposited in a small, shallow freshwater lake within an oxygen-rich and relatively wet and hot paleoclimate, whereas the shales in the Upper Member were deposited in a large, deep, and saline water lake with dysoxic-anoxic conditions and a relatively arid and cold paleoclimate. The organic matter content in the Lower Member was primarily determined by the amount of detritus input with a low paleo-productivity owing to a strong weathering, whereas that in the Upper Member was dominated by anoxic processes under a good preservation and a low deposition rate. The model reflecting the relationship between accommodation and sediment-fill is employed to analyse the mechanism of organic matter accumulation. Three depositional stages are distinguished within the Shimengou Formation corresponding to overfilled, underfilled, and balanced-filled sediments with average TOC contents of 0.84 %, 4.95 %, and 6.09 %, respectively. This study reveals the dominated factors controlling organic matter enrichment in a continental basin and proposes a TOC distribution mechanism, which could provide a guide for the shale gas and shale oil exploration. • High-resolution geochemistry and detailed geological description were performed. • Paleoclimate evolution of Middle Jurassic Shimengou Formation was analysed. • Detritus inputs and anoxic degree were predominant factors for influencing TOC. • Three depositional stages of the Shimengou Formation were subdivided. • The model of lacustrine organic matter accumulation was proposed.