Low-latitude Populations Research Articles

The Late Pleistocene Canis lupus (Canidae, Mammalia) from Avetrana (Apulia, Italy): reappraisal and new insights on the European glacial wolves

The modern wolf, Canis lupus Linnaeus, 1758, has one of the largest ranges amongst carnivorans, and for this reason it shows local and regional differences for adaptation to a great variety of habitats, ranging from the arctic tundra to the Arabian desert. These differences are particularly evident as wolves follow the Bergmann's ecogeographical rule, with low latitude populations being generally smaller than those living at high latitudes. The fossil record of the modern wolf dates back to the second half of the Middle Pleistocene. The earliest records come from the French site Lunel-Viel 1 and are ascribed to the subspecies Canis lupus lunellensis Bonifay, 1971, and from the Italian Polledrara di Cecanibbio. These Middle Pleistocene forms were generally small in size, slightly larger than the last representative of Canis mosbachensis Soergel, 1925. During the last 400 kyr, European continental environments were profoundly affected by the glacial/interglacial cycles and much evidence suggests a trend toward a size increase for C. lupus. In particular, a number of these European large-sized wolves seem to be typical of cold phases, for example, Canis lupus maximus Boudadi-Maligne, 2012 from the OIS 3-2. The aim of the present study was to describe the morphological and morphometric variability of C. lupus from Avetrana bed 8 in comparison to other populations from northern and southern Italy, as well as from other localities in Europe, to obtain a better understanding of the biochronology, palaeobiogeography and evolution of this large carnivore in the last 125 kyr. The morphological and morphometric analyses pointed out the difference of C. lupus of Avetrana compared to the Apulian and to the Northern Italian wolves but also identified a marked affinity with the C. lupus of the glacial site Cardamone (Apulia), referred to OIS 2. This similarity support the idea of the dispersal of large morphotypes (glacial wolves) during cold phases.

Latitudinal and Temperature-Dependent Variation in Embryonic Development Rate and Offspring Performance in a Freshwater Turtle.

The thermal environment of embryos differs significantly along a latitudinal cline, and the mechanism by which embryos respond to this geographic temperature variation has attracted increasing attention recently. Here, we carried out a common-garden experiment of egg incubation at two fluctuating temperature regimes to elucidate the latitudinal pattern and thermal dependence of the embryonic development rate and offspring performance in the Chinese soft-shelled turtle (Pelodiscus sinensis). Our results demonstrated significant temperature-by-population interactions on variations in the incubation period and hatchling righting response. The incubation period was shorter and the daily number of heart beats was higher in the medium- and high-latitude populations than in the low-latitude population at low incubation temperatures but not at high temperatures. Offspring from the medium- and high-latitude populations showed a faster righting response than those from the low-latitude population when incubated at low temperatures, whereas offspring from the high-latitude population showed a faster righting response than those from the medium-latitude population when incubated at high temperatures. This indicates that turtle embryos from different latitudinal locations may have evolved divergent thermal sensitivities of embryonic development in response to their respective nest environments.

Latitudinal variation in body size in Fejervarya limnocharis supports the inverse of Bergmann’s rule

Abstract Bergmann’s rule states that within a species of endotherms smaller individuals are found in warmer conditions, which is consistent for nearly all endotherms, while in ectotherms body size patterns are less consistent. As ectothermic vertebrates, the morphology of amphibians is likely impacted by climatic conditions. Here, we examined latitudinal variation in body size in the ranid frog, Fejervarya limnocharis, based on literature and our own data on mean body size of 3637 individuals from 50 populations and average age of 2873 individuals from 40 populations in China. The results showed that body size was positively correlated with environmental temperature, but not with precipitation. Body size was negatively correlated with latitude among populations in this species, which supported the inverse of Bergmann’s rule. Our findings suggest that a larger body size in low-latitude populations is associated with a longer growing season related to the higher environmental temperature.

A large predatory reef fish species moderates feeding and activity patterns in response to seasonal and latitudinal temperature variation

Climate-driven increases in ocean temperatures are expected to affect the metabolic requirements of marine species substantially. To mitigate the impacts of increasing temperatures in the short-term, it may be necessary for ectothermic organisms to alter their foraging behaviour and activity. Herein, we investigate seasonal variation in foraging behaviour and activity of latitudinally distinct populations of a large coral reef predator, the common coral trout, Plectropomus leopardus, from the Great Barrier Reef, Australia. P. leopardus exhibited increased foraging frequency in summer versus winter time, irrespective of latitude, however, foraging frequency substantially declined at water temperatures >30 °C. Foraging frequency also decreased with body size but there was no interaction with temperature. Activity patterns were directly correlated with water temperature; during summer, the low-latitude population of P. leopardus spent up to 62% of their time inactive, compared with 43% for the high-latitude population. The impact of water temperature on activity patterns was greatest for larger individuals. These results show that P. leopardus moderate their foraging behaviour and activity according to changes in ambient temperatures. It seems likely that increasing ocean temperatures may impose significant constraints on the capacity of large-bodied fishes to obtain sufficient prey resources while simultaneously conserving energy.

Stressful summers? Torpor expression differs between high- and low-latitude populations of bats

Variation in weather and food availability impacts the energy budgets of endotherms, with some species using torpor as an energy-saving strategy during periods of negative energy balance. We evaluated how latitudinal differences in energy balance relate to variation in torpor expression and roosting sociality between populations. We monitored summer skin temperatures (Tsk) of individuals from 2 populations of New Zealand lesser short-tailed bats (Mystacina tuberculata) separated by 6° of latitude using temperature telemetry. Although mean summer Ta was only < 1°C lower for the higher-latitude than lower-latitude population, individuals living at the higher-latitude site used torpor on 36% of observation days compared to 11% for lower-latitude bats. None of the recorded weather variables affected the propensity to enter torpor or torpor bout duration; however, the minimum torpid Tsk of bats positively correlated with daily minimum Ta. Roosts occupied by solitary bats were warmer than Ta, and temperatures within them fluctuated less than Ta. Higher-latitude individuals roosted solitarily (38%) more frequently than lower-latitude individuals (17%) and individuals from both populations exclusively used torpor while roosting solitarily. Arousals from torpor by higher-latitude bats coincided with sunset and not daily Ta maxima suggesting that bats were not fully exploiting advantages of passive rewarming. Site-specific roost choice and torpor patterns were apparent between M. tuberculata populations during summer, demonstrating that small differences in Ta differentially affect energetic strategy. The thermoregulatory behavior of species inhabiting latitudinal gradients in climate is highly plastic, likely to meet the specific challenges of their environment.

Latitudinal and age-specific patterns of larval mortality in the damselfly Lestes sponsa: Senescence before maturity?

Latitudinal differences in life history traits driven by differences in seasonal time constraints have been widely documented. Yet, latitudinal patterns in (age-specific) mortality rates have been poorly studied. Here, we studied latitudinal differences in pre-adult age-specific mortality patterns in the strictly univoltine damselfly Lestes sponsa. We compared individuals from three latitudes reared from the egg stage in the laboratory at temperatures and photoperiods simulating those at the latitude of origin (main experiment) and under common-garden conditions at a fixed temperature and photoperiod (supplementary experiment). Results from the main experiment showed that the high-latitude population exhibited higher mortality rates than the central and southern populations, likely reflecting a cost of their faster development. Age-specific mortality patterns, also indicated higher ageing rates in the high-latitude compared to the low-latitude population, which likely had a genetic basis. The strong within-population variation in hatching dates in the low-latitude population caused variation in mortality rates; individuals that hatched later showed higher mortality rates presumably due to their shorter development times compared to larvae that hatched earlier. In both experiments, larvae from all three latitudes showed accelerated mortality rates with age, which is consistent with a pattern of senescence before adulthood.

Adaptation to a latitudinal thermal gradient within a widespread copepod species: the contributions of genetic divergence and phenotypic plasticity.

Understanding how populations adapt to heterogeneous thermal regimes is essential for comprehending how latitudinal gradients in species diversification are formed, and how taxa will respond to ongoing climate change. Adaptation can occur by innate genetic factors, by phenotypic plasticity, or by a combination of both mechanisms. Yet, the relative contribution of such mechanisms to large-scale latitudinal gradients of thermal tolerance across conspecific populations remains unclear. We examine thermal performance in 11 populations of the intertidal copepod Tigriopus californicus, ranging from Baja California Sur (Mexico) to British Columbia (Canada). Common garden experiments show that survivorship to acute heat-stress differs between populations (by up to 3.8°C in LD50 values), reflecting a strong genetic thermal adaptation. Using a split-brood experiment with two rearing temperatures, we also show that developmental phenotypic plasticity is beneficial to thermal tolerance (by up to 1.3°C), and that this effect differs across populations. Although genetic divergence in heat tolerance strongly correlates with latitude and temperature, differences in the plastic response do not. In the context of climate warming, our results confirm the general prediction that low-latitude populations are most susceptible to local extinction because genetic adaptation has placed physiological limits closer to current environmental maxima, but our results also contradict the prediction that phenotypic plasticity is constrained at lower latitudes.

Optimal defense theory explains deviations from latitudinal herbivory defense hypothesis.

The latitudinal herbivory defense hypothesis (LHDH) postulates that the prevalence of species interactions, including herbivory, is greater at lower latitudes, leading to selection for increased levels of plant defense. While latitudinal defense clines may be caused by spatial variation in herbivore pressure, optimal defense theory predicts that clines could also be caused by ecogeographic variation in the cost of defense. For instance, allocation of resources to defense may not increase plant fitness when growing seasons are short and plants must reproduce quickly. Here we use a common garden experiment to survey genetic variation for constitutive and induced phenylpropanoid glycoside (PPG) concentrations across 35 Mimulus guttatus populations over a ~13° latitudinal transect. Our sampling regime is unique among studies of the LHDH in that it allows us to disentangle the effects of growing season length from those of latitude, temperature, and elevation. For five of the seven PPGs surveyed, we find associations between latitude and plant defense that are robust to population structure. However, contrary to the LHDH, only two PPGs were found at higher levels in low latitude populations, and total PPG concentrations were higher at higher latitudes. PPG levels are strongly correlated with growing season length, with higher levels of PPGs in plants from areas with longer growing seasons. Further, flowering time is positively correlated with the concentration of nearly all PPGs, suggesting that there may be a strong trade-off between development time and defense production. Our results reveal that ecogeographic patterns in plant defense may reflect variation in the cost of producing defense compounds in addition to variation in herbivore pressure. Thus, the biogeographic pattern predicted by the LHDH may not be accurate because the underlying factors driving variation in defense, in this case, growing season length, are not always associated with latitude in the same manner. Given these results, we conclude that LHDH cannot be interpreted without considering life history, and we recommend that future work on the LHDH move beyond solely testing the core LHDH prediction and place greater emphasis on isolating agents of selection that generate spatial variation in defense and herbivore pressure.

Strong Delayed Interactive Effects of Metal Exposure and Warming: Latitude-Dependent Synergisms Persist Across Metamorphosis.

As contaminants are often more toxic at higher temperatures, predicting their impact under global warming remains a key challenge for ecological risk assessment. Ignoring delayed effects, synergistic interactions between contaminants and warming, and differences in sensitivity across species' ranges could lead to an important underestimation of the risks. We addressed all three mechanisms by studying effects of larval exposure to zinc and warming before, during, and after metamorphosis in Ischnura elegans damselflies from high- and low-latitude populations. By integrating these mechanisms into a single study, we could identify two novel patterns. First, during exposure zinc did not affect survival, whereas it induced mild to moderate postexposure mortality in the larval stage and at metamorphosis, and very strongly reduced adult lifespan. This severe delayed effect across metamorphosis was especially remarkable in high-latitude animals, as they appeared almost insensitive to zinc during the larval stage. Second, the well-known synergism between metals and warming was manifested not only during the larval stage but also after metamorphosis, yet notably only in low-latitude damselflies. These results highlight that a more complete life-cycle approach that incorporates the possibility of delayed interactions between contaminants and warming in a geographical context is crucial for a more realistic risk assessment in a warming world.

Integrating both interaction pathways between warming and pesticide exposure on upper thermal tolerance in high- and low-latitude populations of an aquatic insect

Global warming and chemical pollution are key anthropogenic stressors with the potential to interact. While warming can change the impact of pollutants and pollutants can change the sensitivity to warming, both interaction pathways have never been integrated in a single experiment. Therefore, we tested the effects of warming and multiple pesticide pulses (allowing accumulation) of chlorpyrifos on upper thermal tolerance (CTmax) and associated physiological traits related to aerobic/anaerobic energy production in the damselfly Ischnura elegans. To also assess the role of latitude-specific thermal adaptation in shaping the impact of warming and pesticide exposure on thermal tolerance, we exposed larvae from replicated high- and low-latitude populations to the pesticide in a common garden rearing experiment at 20 and 24 °C, the mean summer water temperatures at high and low latitudes. As expected, exposure to chlorpyrifos resulted in a lower CTmax. Yet, this pesticide effect on CTmax was lower at 24 °C compared to 20 °C because of a lower accumulation of chlorpyrifos in the medium at 24 °C. The effects on CTmax could partly be explained by reduction of the aerobic scope. Given that these effects did not differ between latitudes, gradual thermal evolution is not expected to counteract the negative effect of the pesticide on thermal tolerance. By for the first time integrating both interaction pathways we were not only able to provide support for both of them, but more importantly demonstrate that they can directly affect each other. Indeed, the warming-induced reduction in pesticide impact generated a lower pesticide-induced climate change sensitivity (in terms of decreased upper thermal tolerance). Our results indicate that, assuming no increase in pesticide input, global warming might reduce the negative effect of multiple pulse exposures to pesticides on sensitivity to elevated temperatures.

Local adaptation of reproductive performance during thermal stress.

Considerable evidence exists for local adaptation of critical thermal limits in ectotherms following adult temperature stress, but fewer studies have tested for local adaptation of sublethal heat stress effects across life-history stages. In organisms with complex life cycles, such as holometabolous insects, heat stress during juvenile stages may severely impact gametogenesis, having downstream consequences on reproductive performance that may be mediated by local adaptation, although this is rarely studied. Here, we tested how exposure to either benign or heat stress temperature during juvenile and adult stages, either independently or combined, influences egg-to-adult viability, adult sperm motility and fertility in high- and low-latitude populations of Drosophila subobscura. We found both population- and temperature-specific effects on survival and sperm motility; juvenile heat stress decreased survival and subsequent sperm motility and each trait was lower in the northern population. We found an interaction between population and temperature on fertility following application of juvenile heat stress; although fertility was negatively impacted in both populations, the southern population was less affected. When the adult stage was also subject to heat stress, the southern population exhibited positive carry-over effects whereas the northern population's fertility remained low. Thus, the northern population is more susceptible to sublethal reproductive consequences following exposure to juvenile heat stress. This may be common in other organisms with complex life cycles and current models predicting population responses to climate change, which do not take into account the impact of juvenile heat stress on reproductive performance, may be too conservative.

Will phenotypic plasticity affecting flowering phenology keep pace with climate change?

Rising temperatures have begun to shift flowering time, but it is unclear whether phenotypic plasticity can accommodate projected temperature change for this century. Evaluating clines in phenological traits and the extent and variation in plasticity can provide key information on assessing risk of maladaptation and developing strategies to mitigate climate change. In this study, flower phenology was examined in 52 populations of big sagebrush (Artemisia tridentata) growing in three common gardens. Flowering date (anthesis) varied 91days from late July to late November among gardens. Mixed-effects modeling explained 79% of variation in flowering date, of which 46% could be assigned to plasticity and genetic variation in plasticity and 33% to genetics (conditional R2 =0.79, marginal R2 =0.33). Two environmental variables that explained the genetic variation were photoperiod and the onset of spring, the Julian date of accumulating degree-days >5°C reaching 100. The genetic variation was mapped for contemporary and future climates (decades 2060 and 2090), showing flower date change varies considerably across the landscape. Plasticity was estimated to accommodate, on average, a ±13-day change in flowering date. However, the examination of genetic variation in plasticity suggests that the magnitude of plasticity could be affected by variation in the sensitivity to photoperiod and temperature. In a warmer common garden, lower-latitude populations have greater plasticity (+16days) compared to higher-latitude populations (+10days). Mapped climatypes of flowering date for contemporary and future climates illustrate the wide breadth of plasticity and large geographic overlap. Our research highlights the importance of integrating information on genetic variation, phenotypic plasticity and climatic niche modeling to evaluate plant responses and elucidate vulnerabilities to climate change.

The Adaptive Significance of Natural Genetic Variation in the DNA Damage Response of Drosophila melanogaster.

Despite decades of work, our understanding of the distribution of fitness effects of segregating genetic variants in natural populations remains largely incomplete. One form of selection that can maintain genetic variation is spatially varying selection, such as that leading to latitudinal clines. While the introduction of population genomic approaches to understanding spatially varying selection has generated much excitement, little successful effort has been devoted to moving beyond genome scans for selection to experimental analysis of the relevant biology and the development of experimentally motivated hypotheses regarding the agents of selection; it remains an interesting question as to whether the vast majority of population genomic work will lead to satisfying biological insights. Here, motivated by population genomic results, we investigate how spatially varying selection in the genetic model system, Drosophila melanogaster, has led to genetic differences between populations in several components of the DNA damage response. UVB incidence, which is negatively correlated with latitude, is an important agent of DNA damage. We show that sensitivity of early embryos to UVB exposure is strongly correlated with latitude such that low latitude populations show much lower sensitivity to UVB. We then show that lines with lower embryo UVB sensitivity also exhibit increased capacity for repair of damaged sperm DNA by the oocyte. A comparison of the early embryo transcriptome in high and low latitude embryos provides evidence that one mechanism of adaptive DNA repair differences between populations is the greater abundance of DNA repair transcripts in the eggs of low latitude females. Finally, we use population genomic comparisons of high and low latitude samples to reveal evidence that multiple components of the DNA damage response and both coding and non-coding variation likely contribute to adaptive differences in DNA repair between populations.

The relationship of telomere length to baseline corticosterone levels in nestlings of an altricial passerine bird in natural populations.

BackgroundEnvironmental stressors increase the secretion of glucocorticoids that in turn can shorten telomeres via oxidative damage. Modification of telomere length, as a result of adversity faced early in life, can modify an individual’s phenotype. Studies in captivity have suggested a relationship between glucocorticoids and telomere length in developing individuals, however less is known about that relationship in natural populations.MethodsIn order to evaluate the effect of early environmental stressors on telomere length in natural populations, we compared baseline corticosterone (CORT) levels and telomere length in nestlings of the same age. We collected blood samples for hormone assay and telomere determination from two geographically distinct populations of the Thorn-tailed Rayadito (Aphrastura spinicauda) that differed in brood size; nestlings body mass and primary productivity. Within each population we used path analysis to evaluate the relationship between brood size, body mass, baseline CORT and telomere length.ResultsWithin each distinct population, path coefficients showed a positive relationship between brood size and baseline CORT and a strong and negative correlation between baseline CORT and telomere length. In general, nestlings that presented higher baseline CORT levels tended to present shorter telomeres. When comparing populations it was the low latitude population that presented higher levels of baseline CORT and shorter telomere length.ConclusionsTaken together our results reveal the importance of the condition experienced early in life in affecting telomere length, and the relevance of integrative studies carried out in natural conditions.

Variation in the ontogeny of sex steroid levels between latitudinal populations of the medaka.

IntroductionSex steroids mediate the expression of sexual dimorphism during ontogeny, and populations that differ in the magnitudes of sexual dimorphism may accordingly differ in the ontogenetic patterns of their sex steroid levels. The medaka, Oryzias latipes species complex, shows geographic variation in the magnitude of sexual dimorphism with respect to the lengths of their anal and dorsal fins; dimorphism is greater in low-latitude populations than in high-latitude populations. However, sexual differences in the ontogenetic dynamics of sex steroids, and its interpopulation variation, have not been examined.ResultsWe measured testosterone (T), estradiol-17β (E2), and 11-ketotestosterone (11-KT) concentrations throughout ontogeny of laboratory-reared fish from two latitudinal populations: Aomori (northern) and Okinawa (southern). In both populations, the levels of all three steroids were high during early ontogenetic stages and decreased with growth. After reaching about 15 mm in standard length, when sexual dimorphisms in fin lengths became apparent, steroid levels increased and tended to plateau. Sexual differences in the steroid levels were observed only in the later ontogenetic stages; T and 11-KT levels were higher in males, while E2 levels were higher in females. Accordingly, interpopulation differences also became clearer; the southern fish tended to show higher T levels and lower E2 levels than the northern fish.ConclusionsThe ontogenetic patterns of sex steroid levels paralleled the ontogeny of anal and dorsal fins in the two latitudinal populations, suggesting that interpopulation variation in the degree of sexual dimorphisms in fin lengths is mediated by sex steroid-dependent regulation of fin elongation.

Seasonal time constraints reduce genetic variation in life‐history traits along a latitudinal gradient

Time constraints cause strong selection on life-history traits, because populations need to complete their life cycles within a shorter time. We therefore expect lower genetic variation in these traits in high- than in low-latitude populations, since the former are more time-constrained. The aim was to estimate life-history traits and their genetic variation in an obligately univoltine damselfly along a latitudinal gradient of 2730km. Populations were grown in the laboratory at temperatures and photoperiods simulating those at their place of origin. In a complementary experiment, individuals from the same families were grown in constant temperature and photoperiod that mimicked average conditions across the latitude. Development time and size was faster and smaller, respectively, and growth rate was higher at northern latitudes. Additive genetic variance was very low for life-history traits, and estimates for egg development time and larval growth rate showed significant decreases towards northern latitudes. The expression of genetic effects in life-history traits differed considerably when individuals were grown in constant rather than simulated and naturally variable conditions. Our results support strong selection by time constraints. They also highlight the importance of growing organisms in their native environment for correct estimates of genetic variance at their place of origin. Our results also suggest that the evolutionary potential of life-history traits is very low at northern compared to southern latitudes, but that changes in climate could alter this pattern.

Variation in male courtship activeness between latitudinal populations of Northern medaka

It has been demonstrated from free mating experiments using multiple males and females of the medaka, Oryzias latipes species complex, that lower-latitude males exhibit more active courtship behavior than higher-latitude males. In this study, courtship activeness in males from two latitudinal populations of Northern medaka, Oryzias sakaizumii, was measured in the presence of only one male and one female, where male–male interactions were completely eliminated. We found that males from the low-latitude population performed quick circles (i.e., courtship dance) and approached females more frequently than males from the high-latitude population. Moreover, the low-latitude males tended to start courtships sooner after encountering a female than the high-latitude males. Thus, the latitudinal variation in male courtship activeness persisted even in the absence of rival males. These probably reflect that sexual selection pressures are stronger at low latitudes.

Warmer winters modulate life history and energy storage but do not affect sensitivity to a widespread pesticide in an aquatic insect

Despite the increased attention for the effects of pesticides under global warming no studies tested how winter warming affects subsequent sensitivity to pesticides. Winter warming is expected to cause delayed negative effects when it increases metabolic rates and thereby depletes energy reserves. Using a common-garden experiment, we investigated the combined effect of a 4°C increase in winter temperature and subsequent exposure to chlorpyrifos in the aquatic larvae of replicated low- and high-latitude European populations of the damselfly Ischnura elegans. The warmer winter (8°C) resulted in a higher winter survival and higher growth rates compared to the cold winter (4°C) commonly experienced by European high-latitude populations. Low-latitude populations were better at coping with the warmer winter, indicating thermal adaptation to the local winter temperatures. Subsequent chlorpyrifos exposure at 20°C induced strong negative effects on survival, growth rate, lipid content and acetylcholinesterase activity while phenoloxidase activity increased. These pesticide effects were not affected by winter warming. Our results suggest that for species where winter warming has positive effects on life history, no delayed effects on the sensitivity to subsequent pesticide exposure should be expected.

Parallel Gene Expression Differences between Low and High Latitude Populations of Drosophila melanogaster and D. simulans.

Gene expression variation within species is relatively common, however, the role of natural selection in the maintenance of this variation is poorly understood. Here we investigate low and high latitude populations of Drosophila melanogaster and its sister species, D. simulans, to determine whether the two species show similar patterns of population differentiation, consistent with a role for spatially varying selection in maintaining gene expression variation. We compared at two temperatures the whole male transcriptome of D. melanogaster and D. simulans sampled from Panama City (Panama) and Maine (USA). We observed a significant excess of genes exhibiting differential expression in both species, consistent with parallel adaptation to heterogeneous environments. Moreover, the majority of genes showing parallel expression differentiation showed the same direction of differential expression in the two species and the magnitudes of expression differences between high and low latitude populations were correlated across species, further bolstering the conclusion that parallelism for expression phenotypes results from spatially varying selection. However, the species also exhibited important differences in expression phenotypes. For example, the genomic extent of genotype × environment interaction was much more common in D. melanogaster. Highly differentiated SNPs between low and high latitudes were enriched in the 3’ UTRs and CDS of the geographically differently expressed genes in both species, consistent with an important role for cis-acting variants in driving local adaptation for expression-related phenotypes.

Photoprotective response to chilling differs among high and low latitude Larrea divaricata grown in a common garden

Cold temperatures can cause imbalances between light absorption and photosynthetic light utilization, with potential negative consequences for leaf function. Consequently, in response to chilling evergreens typically exhibit increased total pools of photoprotective xanthophyll cycle pigments and greater nocturnal retention of energy dissipating forms. This may preclude opportunistic responses to transient warmer temperatures, however, and become less beneficial as cold spells become rare and/or short-lived, for example, at the low latitude edges of warm deserts. Thus, we hypothesized intrinsic differences in the photoprotective response to chilling between low and high latitude populations of the widespread warm desert evergreen shrub Larrea divaricata. We determined leaf pigment compositions for high and low latitude plants grown in a common garden before and after 7 days of chilling (12/1 °C day/night). High and low latitude plants differed in pigment composition before chilling, and plants from both populations adjusted pigment compositions in response to chilling. Chilling, however, induced significantly greater nocturnal retention of xanthophyll cycle pigments as zeaxanthin and antheraxanthin in high latitude plants compared to those from low latitude. These data provide strong evidence for intrinsic differences between high and low latitude populations in their photoprotective responses to both the greenhouse growth environment and to chilling.