Low Grade Research Articles

Design method for multi-stage fresh air treatment system with high seasonal energy efficiency

Compared to traditional air conditioning systems that only use high-grade chilled/hot water, multi-stage air treatment (MAT) systems use lower grade chilled/hot water and achieve higher energy efficiency. However, the area of heat exchangers in the existing MAT system is designed based on the maximum value of all working conditions, which is large and not appropriate for partial load conditions. Therefore, this study proposes a method for MAT system design wherein the area of heat exchangers can be appropriate for different working conditions. In this study, optimal area ranges for various working conditions in a fresh air system are calculated, and the overlapping range of these optimal area ranges for all working conditions is determined. Then, the performance of the proposed MAT system is numerically calculated, and six different climate zones are selected to evaluate the applicability of the proposed design methods. The results show that: (1) when the system electric power fluctuates within a small range of 3%, the designed area of heat exchangers can vary over a large range of approximately 50% to 150%; (2) compared to the existing MAT system, the designed area of heat exchangers in the proposed MAT system is reduced by 50.0%, and the maximum energy saving rates in the cooling and heating seasons are 10.9% and 9.8%, respectively; (3) in the selected six climate zones, the duration ratio of working conditions where the designed area is not within the optimal range is less than 5%, indicating good applicability of the proposed design method.

Comparison of liposomal gel with and without addition of chamomile for prevention of radiation dermatitis in head and neck cancer patients: A randomized controlled trial

Background and purposeHead and neck cancer patients frequently develop radiation dermatitis (RD) during radiotherapy. We compared liposomal gel with and without chamomile extract for the prevention and management of RD in head and neck cancer patients undergoing radiotherapy. Materials and methodsSixty participants undergoing radiotherapy for the first time for head and neck cancer were recruited in a double-blind randomized clinical trial. Primary outcomes were the occurrence of dry desquamation and the cumulative dose of ionizing radiation at the first occurrence of dry desquamation. Secondary outcomes were the occurrence of erythema, moist desquamation, global RD, and participant self-reported symptoms. ResultsDry desquamation occurred in 76.9 % (95 % CI: 57.9, 89.0) of participants in the chamomile liposomal gel group and in 88.9 % (95 % CI: 71.9, 96.1) in the liposomal gel group (p = 0.43). The median cumulative dose of ionizing radiation when dry desquamation occurred was 48.0 Gy in the chamomile liposomal gel group and 40.0 Gy in the liposomal gel group. Fewer symptoms were reported in the chamomile liposomal gel group. ConclusionNo statistically significant differences in outcomes were found between the two groups, though the chamomile liposomal gel group presented with lower grades of RD than the liposomal gel group. In addition, when compared to the literature on other topical interventions to manage RD, our results show that chamomile liposomal gel has potential for better management and prevention of RD in this population, which should be further tested. This study provides crucial information to design future studies.Clinical Trials Registration (REBEC): RBR-92cts3.

Spatial distribution of tumour immune infiltrate predicts outcomes of patients with high-risk soft tissue sarcomas after neoadjuvant chemotherapy

BackgroundAnthracycline-based neoadjuvant chemotherapy (NAC) may modify tumour immune infiltrate. This study characterized immune infiltrate spatial distribution after NAC in primary high-risk soft tissue sarcomas (STS) and investigate association with prognosis. MethodsThe ISG-STS 1001 trial randomized STS patients to anthracycline plus ifosfamide (AI) or a histology-tailored (HT) NAC. Four areas of tumour specimens were sampled: the area showing the highest lymphocyte infiltrate (HI) at H&E; the area with lack of post-treatment changes (highest grade, HG); the area with post-treatment changes (lowest grade, LG); and the tumour edge (TE). CD3, CD8, PD-1, CD20, FOXP3, and CD163 were analyzed at immunohistochemistry and digital pathology. A machine learning method was used to generate sarcoma immune index scores (SIS) that predict patient disease-free and overall survival (DFS and OS). FindingsTumour infiltrating lymphocytes and PD-1+ cells together with CD163+ cells were more represented in STS histologies with complex compared to simple karyotype, while CD20+ B-cells were detected in both these histology groups. PD-1+ cells exerted a negative prognostic value irrespectively of their spatial distribution. Enrichment in CD20+ B-cells at HI and TE areas was associated with better patient outcomes. We generated a prognostic SIS for each tumour area, having the HI-SIS the best performance. Such prognostic value was driven by treatment with AI. InterpretationThe different spatial distribution of immune populations and their different association with prognosis support NAC as a modifier of tumour immune infiltrate in STS.Funding: Pharmamar; Italian Ministry of Health [RF-2019-12370923; GR-2016-02362609]; 5x1000 Funds—2016, Italian Ministry of Health; AIRC Grant [ID#28546].

Entry into public higher education - differences highlighted with UFPE data between open competition and quota students

The Quota Law for Higher Education in Brazil, implemented as in the entry of 2013, was designed to facilitate access for students from public schools to higher education, in universities and federal institutes, and promote social inclusion. The law provided that within ten years, counting from the date of publication, a review of the special program for access to higher education institutions would be promoted. This work seeks to contribute to the analysis of law enforcement, especially by observing admission scores comparing quota and non-quota students. We analyzed data from students entering courses in the Basic Area of Entry (ABI – Engineering), Law and Medicine at UFPE, at Campus Recife, which are courses in different areas of knowledge. To analyze performance in the admission process, based on the SiSU grades, all students entering the aforementioned courses in the period from 2016.1 to 2020.2 were considered, totalizing 5145 students. The results reveal that there is evidence that the SiSU grades of students in open competition are higher than the grades of quota students, for the three courses analyzed (Medicine, Law and ABI-Engineering at UFPE), throughout the study period, namely 2016.1 to 2020.2, and also that among the quota students, those self-declared as black, brown or indigenous entered with lower average grades than the other quota students, in all years.

Undergraduate Goal Orientations Across the Globe: Does the 3 X 2 Model Hold Up?

<p style="text-align:justify">Recently, a large-scale study of college students’ motivation orientations when they transitioned to online learning because of the global COVID-19 pandemic found that unlike prior studies, in the 3 x 2 goal orientation framework the standard for competence (self, other, task) was of greater importance than valence (approach, avoidant). Moreover, previous research found students’ different goal orientations related to how they responded to the shutdown academically from both volition and social perspectives. We investigated whether a three-factor model would replicate with unique undergraduate cross-cultural samples, and we wanted to examine how students with different goal orientations responded to the shutdown of their universities due to the pandemic. Students from a U.S. university and students from Oman completed a 44-item goal orientation survey, a demographic survey, and an 11-item survey to assess students’ experiences following the pandemic. Results indicate that students who set task-based goals were less likely to have negative experiences related to the social aspects of the pandemic and those students who had negative responses to the lack of social contact were also expected to have lower grades.</p>

The Belingwe greenstone belt, Zimbabwe – some new age constraints and implications for greenstone belt evolution

ABSTRACT The Neoarchaean Belingwe greenstone belt of the Zimbabwe craton consists of a well-preserved succession of rocks of low metamorphic grade and low strain. We present new age constraints for the Ngezi Group of the belt and discuss them in the light of greenstone belt evolution. We propose crustal destabilization of the Zimbabwe proto-craton as a result of a global flare of mantle-derived magmatism at ~ 2.75 Ga. This gave rise to extension of a Mesoarchaean granitoid-greenstone terrain, rapid subsidence and, initially, deposition of a transgressive cover succession (Manjeri Formation). High degrees of stretching of proto-cratonic and mantle lithosphere provided accommodation space for the emplacement of several kilometres of submarine volcanic rocks on deeply submerged granitoid-greenstone basement, herein referred to as proto-cratonic flood basalts. Once extension and magmatism had ceased, chemical and clastic sedimentary rocks of the Cheshire Formation were deposited prior to 2.71 Ga as a result of, and subjected to, compressional deformation, and much earlier than previously assumed.

Optimizing single blastocyst selection: the role of day 3 embryo morphology in vitrified-warmed blastocyst transfer cycles

Can day 3 embryo morphology serve as an independent criterion for optimal single blastocyst selection? This retrospective, single-centre cohort study included 1517 single vitrified-warmed blastocyst transfer (SVBT) cycles conducted between October 2019 and July 2022. The live birth rate (LBR) and other clinical outcomes of SVBT cycles were evaluated, considering both good-quality and non-good-quality day 3 embryos. The associations of day 3 morphological characteristics, encompassing number of blastomeres and embryo grade, were assessed. Multivariable analyses were undertaken using multiple models adjusted for day of blastocyst development and blastocyst grade. Blastocysts from good-quality day 3 embryos had significantly higher LBR compared with those from non-good-quality embryos for both day 5 (51.5% versus 42.9%; P = 0.013) and day 6 (25.1% versus 17.6%; P = 0.018) blastocysts. LBR did not differ significantly with number of blastomeres on day 3, regardless of day of blastocyst development (day 5/6) or blastocyst grade. LBR varied significantly by day 3 embryo grade for both day 5 (48.0%, 51.5%, 46.6% and 32.7% for grades I, II, III and IV-V; P = 0.005) and day 6 (41.5%, 23.6%, 15.9% and 16.1% for grades I, II, III and IV-V; P = 0.001) blastocysts. Multivariable logistic regression revealed that non-good-quality embryos and lower morphological grade (IV-V) on day 3 were significantly and negatively correlated with LBR, while the number of blastomeres on day 3 was not an independent factor. When selecting blastocysts of equal quality for SVBT cycles, those with higher day 3 morphological scores are preferred. Day 3 morphological evaluation is a valuable supplement to conventional selection methods.

The impact of extensive intraductal component (EIC) on the genomic risk of recurrence in early hormone receptor positive breast cancer

BackgroundEarly invasive ductal carcinoma (IDC) breast cancer often presents with a coexisting ductal carcinoma in situ (DCIS) component, while about 5 % of cases present with an extensive (>25 %) intraductal component (EIC). The impact of EIC on the genomic risk of recurrence is unclear. MethodsPatients with early hormone receptor-positive HER2neu-negative (HR + HER2-) IDC breast cancer and a known OncotypeDX Breast Recurrence Score® (RS) who underwent breast surgery at our institute were included. Using a rule-based text-analysis algorithm, we analyzed pathological reports and categorized patients into three groups: EIC, non-extensive DCIS (DCIS-L), and pure-IDC (NO-DCIS). Genomic risk was determined using OncotypeDX RS. ResultsA total of 33 (4.6 %) EIC cases, 377 (57.2 %) DCIS-L cases and 307 (42.8 %) NO-DCIS cases were identified. Patients in the EIC group were younger and had lower tumor grades than other groups. The distribution of genomic risk varied between the groups, with EIC tumors significantly less likely to have a high RS (>25) compared to DCIS-L and No-DCIS tumors (3 % vs 20 % and 20 %, respectively; p = 0.03). When adjusted to age, tumor size, grade and LNs involvement, both DCIS-L and NO-DCIS groups were significantly correlated with a higher probability of high RS compared to the EIC group (OR 12.3 and OR 13.1, respectively; p < 0.02). Moreover, patients with EIC had a lower likelihood for adjuvant chemotherapy recommendation. ConclusionsIn early HR + HER2- IDC, an EIC correlates with a reduced genomic recurrence risk. The impact on genomic risk seems to be influenced by the extent, not merely the presence, of DCIS.

Histomorphological evaluation of thermal injury following palatoplasty performed with CO2 laser or LigaSure device in dogs with brachycephalic obstructive airway syndrome

The elongated soft palate is an abnormality that characterizes most brachycephalic dogs and contributes to the brachycephalic obstructive airway syndrome (BOAS). Palatoplasty is routinely performed in brachycephalic dogs; several surgical techniques exist. The use of surgical instruments such as monopolar electrocoagulation, CO2 or diode laser, bipolar vessel sealing device and harmonic shears has become routine to reduce the operating time, the intraoperative risk of bleeding and the postoperative oedema. This prospective study aimed to compare the histomorphological effect of a CO2 laser and LigaSure device in palates of dogs undergoing palatoplasty. Twenty owned brachycephalic dogs were included, 10 palatoplasties were performed using CO2 laser and 10 using LigaSure™ device. The dogs were positioned in sternal recumbency. A transoral approach was performed: the elongated soft palate was grasped with Allis forceps and brought rostrally, the palatoplasty was performed using the tonsillar crypts as anatomical landmarks. Surgical specimens were routinely fixed in 10 % formalin. Two sections perpendicular to the surgical margins were trimmed from each sample, paraffin-embedded and stained with hematoxylin and eosin (H&E). Tissue damage induced by the two types of surgical devices was graded (1–4, from minimal to severe) and the depth of thermal injury measured in μm on captured images (using an image analysis program - ImageJ). Mean values and standard deviations (SD) were calculated based on six measurements for each sample.The tissue damage was graded 3.7±0.48 in group LigaSure™ and 2.8±1 in group Laser. The mean depth of thermal injury was 874.94±184.92 μm in the LigaSure™ group and 451,76±137,86 μm in the Laser group. The comparison between the two groups showed significant lower grade and extension of thermal injury in the palate samples obtained with CO2 laser (p<0.05).Additionally, there is a lack of literature that correlates the histological changes with the clinical outcomes of the different palatoplasty methods in brachycephalic dogs. By comparing histological changes and clinical outcomes, we aim to provide valuable insights for optimizing the surgical approach for palatoplasty in brachycephalic dogs, ultimately improving postoperative outcomes for these patients.

Can leagues mitigate the demoralization effect of rank feedback? A randomized controlled trial

In a randomized controlled trial involving hundreds of university students, we provide relative performance feedback specifically designed to reduce low performers’ demoralization, by dynamically assigning students to small leaderboard groups that share a similar score in a semester-long online assignment. Treated students appear 2.6% more likely to go beyond the call-of-duty on their assignment by mid-semester. For low performers, this translates in 0.27 SDs higher exam grades, more stress, increased effort and lower procrastination. High performers are happier, procrastinate less and overachieve in the assignment on which they are ranked, but ultimately also score 0.25 SDs lower exam grades.

Analysis of factors that influence students' lack of reading ability in Indonesian language subjects in elementary schools

This research aims to describe the analytical factors that influence the lack of reading ability of lower grade students in Indonesian language subjects. This research was carried out at SDN I Senaru, Batukoq Hamlet, Senaru Village, Bayan District, North Lombok Regency. The object of research is the factors that cause the lack of reading ability of lower grade students in Class II Elementary School where their age ranges from 7-8 years, namely the transition period after toddlerhood to childhood. The age in this research is what causes the low reading ability in class II. The research method used is qualitative research because the data obtained in the field is more in the form of information and information about the conditions of students as they are in the field using observation, interviews and documentation methods, then looking at the results of the analysis of factors that influence the lack of reading ability of low grade students so that It can be concluded that the lack of reading ability is caused by four factors, namely 1). Physiological Factors, 2). Intelligence Factor, 3). Environmental factors and 4), Psychological factors. Some of these factors cause learning outcomes and learning achievements to be not as effective as other friends who are already fluent in reading and this becomes an obstacle for teachers and others.

A Longitudinal Study of CogEvo's Prediction of Cognitive Decline in Older Adults.

The predictive abilities of computer-based screening devices for early cognitive decline (CD) in older adults have rarely been longitudinally examined. Therefore, this study examined the ability of CogEvo, a short-duration, computer-based cognitive screening device requiring little professional involvement, to predict CD among community-dwelling older adults. We determined whether 119 individuals aged ≥ 65 years living in Japanese rural communities who scored ≥ 24 on the Mini-Mental State Examination (MMSE) at baseline developed CD by annually administering the MMSE to them. CD was defined as an MMSE score of ≤23. At baseline, the overall CogEvo judgment grade, with lower grades indicating better cognitive function, was calculated from the results of various cognitive tasks. Over 2 years, 10 participants developed CD. Participants with grades of 4 had a higher percentage of CD cases than those with grades of ≤3 (p < 0.01). This relationship remained significant after controlling for possible confounders, including the MMSE score at baseline. The sensitivity and specificity of the CogEvo grade cutoff of 4 were 50.0% and 93.6%, respectively. In conclusion, CogEvo may be an efficient tool for identifying individuals at a high risk for dementia. The possibility of missing CD cases should be considered when using CogEvo for screening.

Examining the implementation of the language in education policy in Tana River County: a case of a Pokomo speakers’ school

ABSTRACT The language in education policy (LiEP) in Kenya calls for the use of mother tongue for pre-primary and lower grades of primary education. However, its implementation has been erratic. This study sought to explore the implementation of LiEP in Tsana Primary School, situated in Tana River County, Kenya, where the local language is Kipfokomu. Using an ethnographic approach, the study involved interviews and observations with Grade 3 students, their parents or guardian, and teachers from Grades 1 to 3. Relevant documents were also reviewed, and thematic analysis was conducted on the collected data. The study revealed two main themes: (1) the lack of a supportive environment for Kipfokomu in the classroom, and (2) the presence of Kipfokomu resource materials on the school premises, but their limited availability in the classroom setting. These findings suggest challenges in implementing LiEP, as teachers often prioritise English over the local language. Despite the presence of essential resources intended to support LiEP, the persistent challenges undermine its effectiveness. Understanding these challenges can inform policy improvements and more effective strategies for implementing mother tongue-based education, ensuring that the educational system better serves linguistic and cultural diversity.

Trends in myopia development among Chinese children and adolescents in Xuzhou during one academic year.

This study investigates the prevalence and progression of myopia among primary and secondary school students in Xuzhou City, China, during one academic year. The study employed a prospective research design and utilized a whole-group sampling method to conduct non-cycloplegic spot photo screenings on 37,938 students from 44 primary and secondary schools in Xuzhou City, China. A one-year study was conducted to gather spherical equivalent refraction (SER), and subsequent analysis was carried out to explore the disparities in myopia prevalence among primary and secondary school students within the same academic year, as well as the progression of myopia. During the 2022 academic year, the overall prevalence of myopia in the first and second semesters was 62.6 and 64.2% respectively, indicating an increasing trend. Particularly in primary school (Grades 1-6), the prevalence of myopia increased with higher grade levels, and significant variations in myopia prevalence were observed mainly in grades 1-3 and 7 (p < 0.05). The incidence rate of myopia in middle school remained stable, while in primary school, there was a positive correlation between myopia incidence and the grade level, with the highest rate of 20.1% in grade 6. Among the myopic population, the median value of spherical equivalent refraction slightly decreased between the two semesters. The proportion of high myopia increased among students in grades 5-8. Our study revealed that within one academic year, the prevalence of myopia and the severity of myopia have significantly increased in Xuzhou City, China, accompanied by an increase in the proportion of high myopia. For different grade levels, we should adopt personalized prevention and control measures, with a particular focus on lower grade levels and students who have just entered a new grade.

Integrative analysis of genomic and epigenomic regulation reveals miRNA mediated tumor heterogeneity and immune evasion in lower grade glioma

The expression dysregulation of microRNAs (miRNA) has been widely reported during cancer development, however, the underling mechanism remains largely unanswered. In the present work, we performed a systematic integrative study for genome-wide DNA methylation, copy number variation and miRNA expression data to identify mechanisms underlying miRNA dysregulation in lower grade glioma. We identify 719 miRNAs whose expression was associated with alterations of copy number variation or promoter methylation. Integrative multi-omics analysis revealed four subtypes with differing prognoses. These glioma subtypes exhibited distinct immune-related characteristics as well as clinical and genetic features. By construction of a miRNA regulatory network, we identified candidate miRNAs associated with immune evasion and response to immunotherapy. Finally, eight prognosis related miRNAs were validated to promote cell migration, invasion and proliferation through in vitro experiments. Our study reveals the crosstalk among DNA methylation, copy number variation and miRNA expression for immune regulation in glioma, and could have important implications for patient stratification and development of biomarkers for immunotherapy approaches.

Health-related quality of life profile of newly diagnosed patients with Hodgkin and non-Hodgkin lymphomas: A real-world study including 3922 patients from the French REALYSA cohort

IntroductionConsidering the notable advances made in the treatment of lymphoma, assessment of health-related quality of life (HRQoL) of lymphoma patients has become a critical aspect to consider both in clinical research and routine practice. However, there is paucity of information about lymphoma specific HRQoL profile at diagnosis. Patients and methodsHRQoL at diagnosis was assessed for 3922 adult patients with newly diagnosed high-grade (HG) (n = 1994), low-grade (LG) (n = 1053) non-Hodgkin (NHL) and Hodgkin (HL) (n = 875) lymphomas included in REal world dAta in LYmphoma and Survival in Adults (REALYSA, NCT03869619), a prospective non-interventional multicentric cohort in France. Disease-specific HRQoL aspects were assessed with three validated EORTC questionnaires, namely, the QLQ‐NHL‐HG29, the QLQ-NHL-LG20 and the QLQ-HL27, for patients with NHL-HG, NHL-LG and HL, respectively. ResultsWe confirmed the high-level of completion of these questionnaires in REALYSA cohort, ranging from 84 % for QLQ-HG29 to 88 % for QLQ-HL27. The proportion of patients with impaired global health status was as follows: T-cell NHL, 67 %; diffuse large B-cell (DLBCL), 62 %; Burkitt, 61 %; HL, 53 %; marginal zone, 49 %; mantle cell, 48 %; follicular, 47 %. Multivariable regression analyses for DLBCL, follicular and HL showed that gender, performance status and B symptoms were independently associated with all HRQoL dimensions. However, a variable effect of age and stage were observed among these three subtypes. ConclusionsA comprehensive analysis was made describing the HRQoL profile of newly diagnosed patients with different types of lymphomas. Our data may help to enhance the interpretation of HRQoL results in future studies using the recently validated EORTC lymphoma specific questionnaires.

Does chubby can get lower grades than skinny Sophie? Using an intersectional approach to uncover grading bias in German secondary schools.

We aim to uncover grading bias by gender, socio-economic status, ethnic/migration background as well as body weight in the German secondary school system. Following an intersectional approach, we test whether-controlling for ability-students receive different grades depending on (the specific combination of) ascriptive characteristics. Using data from the fourth starting cohort (SC4, 13.0.0, first survey in year 9 in 2010) of the National Educational Panel Study (NEPS) consisting of more than 14,000 ninth graders, we compute the predicted differences in grades for the different groups of students depending on whether they are a boy or a girl, whether they are obese/overweight or not, their socio-economic status (SES) and ethnic background. We rely on a grade equation approach, assuming that discrepancies between observed grades and achievement as measured in standardised tests are evidence of biased grading. We control for two different competence tests-the Domain General Cognitive Functions (DGCF) and a standardised domain-specific competence test-as objective measures of ability as well as secondary school track. Even after controlling for different personality and behavioural traits-the "big five", the Strengths and Difficulties Questionnaire (SDQ), the Sick, Control, One, Fat and Food (SCOFF), health satisfaction and class retention-substantial differentials in grading across almost all factors and subjects remain. To account for the fact that many students may face bias on multiple grounds, we then compare the differences in predicted grades for groups with overlapping (dis)advantaging characteristics (e.g. low SES overweight Turkish boy vs a high SES non-overweight majority girl), while controlling for the objective ability measures. Significant differentials in grades are found in almost all cases, with the largest effect sizes for the subject German. We also compute models including all 2-way or 4-way interactions between the four axes of inequality and find the main effects largely unchanged. On the whole our findings are indicative of widespread additive intersectional effects of gender, social and ethnic origin as well as body weight on grading bias.

O-249 METAPHOR: METabolic imaging through AI-powered Phasor-based Hyperspectral analysis and Organelle recognition for the classification of human blastocysts

Abstract Study question Can we classify human blastocysts non-invasively using hyperspectral imaging? Summary answer HS imaging can significantly enhance our classification of human embryos and reveal numerous cellular features present in the embryo invisible to brightfield imaging. What is known already Non-invasive measurement of metabolism is a safe and powerful tool to reliably predict oocyte and embryo quality. We have previously shown that our METAPHOR technology, based on HS imaging coupled to artificial intelligence, was able to separate mouse oocyte categories according to maternal age with an average AUC of 96.2% and predict blastocyst formation (AUC 82.2%). Moreover, it was able to classify mouse embryos deprived from different nutrients with an average AUC of 93.7% showing clear superiority to grading provided by experienced embryologists (51%). Study design, size, duration A total of 74 human embryos donated for research at the blastocyst stage have been imaged to date. Embryos were warmed and imaged according to our established protocol and survival was statistically indistinguishable from control embryos. Participants/materials, setting, methods Embryos are imaged using multiphoton illumination near the infra-red. The HS detection covered the simultaneous measurement of the whole visible spectrum. Embryos are then allowed to implant in our proprietary 3D ex vivo platform. Implantation is monitored up until day 9 before immunostaining is performed to quantify presence and prevalence of lineage and implantation markers. Main results and the role of chance After imaging, embryos were placed on the 3D ex vivo implantation platform and let implant. Successful implantation (75.41%) was scored by observation of the characteristic deformation of collagen fibers within the matrix and staining for relevant cell lineages (OCT4, pMLC2). Interestingly, we observed that embryos with a higher trophectoderm (TE) grading tended to implant with a higher efficiency and penetrate deeper into the matrix than embryos with lower grading. HS images have unveiled a profound cellular diversity within embryos, elucidating distinctions between the Inner Cell Mass (ICM) and TE, as well as revealing different metabolic states defined by NADH dominance, FAD dominance, protoporphyrin levels, and apoptosis. HS raw images are processed using our HS-phasor approach which allows quick computing by converting the multidimensional HS data into comprehensive visual representation of bidimensional histograms and phasor plots. In summary, our analysis pipeline encompasses the algorithms to detect and segment the embryos, the computation of histogram and phasor-plot, and the classifier. AI allows us to classify embryos in distinct spectral spaces based on their metabolic features, paving the way to the establishment of a new grading system based on HS imaging. Limitations, reasons for caution The cohort size is still limited, and ongoing experiments will increase the dataset needed for more accurate prediction of embryo implantation. The implantation platform is an in vitro test that will require future clinical validation. Wider implications of the findings The safety and speed of METAPHOR warrants its incorporation at the IVF clinic, adding an additional layer of information to the current embryo selection methods. Moreover, the non-invasive measurement of the metabolic function opens new alleys of research such as the effect of media and culture supplements on embryo culture. Trial registration number Not applicable

Analisis Kesulitan Keterampilan Membaca Dalam mata pelajaran Bahasa Indonesia pada Siswa kelas II Sekolah Dasar

Indonesian language instruction in elementary schools aims to improve students' oral and written communication. Indonesian serves as a primary tool for students to hone their language skills correctly and effectively. Reading skills in elementary school are divided into two phases: initial reading (lower grades) and further reading (upper grades). Initial reading skills influence students' readiness before entering the next learning stage. However, second-grade elementary school students often face difficulties in developing initial reading and writing skills. Factors such as socio-economic background, gender, and teaching methods used by teachers can affect the success of literacy programs. By considering these factors, we can improve students' literacy skills at the beginning level.

Telaah Kurikulum K13 Pendidikan Agama Islam di Madrasah Tsanawiyah Darul Muttaqien

The implementation of the 2013 curriculum began in the 2013 school year with a coba tahap that benefited the more disadvantaged schools. Initially, this curriculum is taught in lower grades, such as grades I and IV for Dasar School, grades VII for Primary School, and grades X for Upper School/Kejuruan School. Over time, this curriculum is needed to cover more ground in various educational settings.One of the primary guidelines in the 2013 curriculum is the application of knowledge to four areas of study: comprehension, application, sikap, and research. The purpose of the curriculum is to evaluate students not only on the basis of their academic performance but also on their moral and social development. This aspek-aspek integration aims to provide a holistic approach to teaching.