High kinetic asymmetry during landing is associated with an increased risk for sustaining a second anterior cruciate ligament (ACL) injury in athletes returning to sport following ACL reconstruction (ACL-R). While previous literature has found that ACL-R patients have more kinetic asymmetry than healthy controls during both landing and bilateral squatting, it is currently unknown if landing asymmetry and squatting asymmetry are related in ACL-R patients. PURPOSE: Determine the relationship between landing kinetic asymmetry and squatting kinetic asymmetry in ACL-R patients. METHODS: 34 ACL-R patients (19 male; 73 ± 16 kg; 174 ± 10 cm; 6.0 ± 1.5 months post-operative) signed informed consent and participated in the study. All participants completed one set of 15 bilateral squats and then ten bilateral stop jump trials, while 3D lower extremity kinematics and kinetics were recorded at 240 Hz and 1920 Hz, respectively. Peak knee extension moment (KEM) and vertical ground reaction force impulse (GRFI) were computed during the descending phase of both tasks. The descending phase was defined between squat initiation and the minimum position of the pelvis during each squat and between initial contact and the minimum position of the pelvis for each stop jump. A limb symmetry index (LSI) was computed for peak KEM and GRFI as the difference between the surgical and non-surgical leg divided by their average during each squat and each stop jump, and then the LSI was averaged across trials. Peak KEM LSI and GRFI LSI were then compared between the stop jump and squat trials using Pearson’s correlations. RESULTS: There was a significant relationship between both stop jump kinetic asymmetry outcomes and both squat kinetic asymmetry outcomes (Table 1). CONCLUSION: Kinetic asymmetry during squatting and bilateral landing are associated in ACL-R patients, which indicates that biofeedback retraining during bilateral squatting could result in improvements in bilateral landing symmetry.