Background: Previous research has shown that increasing cycling cadence can result in improved post-cycling gait velocity. However, the specific threshold of cycling cadence required to bring about clinically meaningful changes in gait velocity remains unknown. This study aimed to determine the minimum increment in cycling cadence that would lead to a significant improvement in post-cycling gait velocity. Methods: A total of 42 young adults participated in our study and were randomly assigned to one of three groups: TEN, TWENTY, and THIRTY. Each group was assigned to cycle at a cadence at the corresponding percentage higher than the participant’s self-selected gait cadence. Each participant engaged in a 15-min cycling session at their respective assigned cycling cadence. Before and after cycling, participants completed a 10-meter walk test while spatiotemporal parameters of gait, ground reaction forces, lower extremity kinematics, and kinetics were recorded. Results: One-way ANOVA revealed no statistically significant changes in spatiotemporal, ground reaction force, kinematics, and kinetics variables pre- and post-cycling. However, there were both statistically significant (F(2,41) = 3.794, p = 0.031, η2 = 0.604) and clinically meaningful changes (0.07 m/s) in post-cycling gait velocity in the THIRTY group only. Conclusions: This suggests that a cycling cadence of 30% or higher is the minimum requirement to produce a clinically significant improvement in gait velocity.
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