Low Depth Research Articles

Exploring the Effect of Aluminium Solute Atoms on Nanomechanical Properties of Copper‐Aluminium System using Nanoindentation

In this work, nanomechanical properties for Cu and Cu–Al (where Al = 2, 4, 6, and 8 wt%) alloys are determined using nanoindentation with Berkovich tip. Nanoindentation experiments are performed using trapezoidal load function where maximum load (Pm) varies from 1000 to 9000 μN at an equal interval of 1000 μN by maintaining equal loading and unloading rate. It is found that Cu8Al sample shows lower depth of penetration (h) at any load (P) in comparison to other samples due to more pinning of dislocations or hinderance of the dislocation movement by solute (Al) atoms during nanoindentation deformation. In addition, it is observed that the recovery is maximum for Cu8Al sample among all samples; however, this recovery is reducing with the increasing Pm for all samples. Thereafter, the elasticity and plasticity index are calculated from the area under the P–h curves, which shows that the elastic index is maximum for Cu8Al and minimum for Cu. Furthermore, the nanomechanical properties (reduced elastic modulus [Er] and hardness [H]) are reported for all samples and significant indentation size effect is observed for Cu8Al due to dislocation structure during deformation.

Fermented aquatic weed meal (FAWM) as a protein source in Asian Catfish Clarias batrachus diets: Impacts on growth, blood chemistry profile, liver and gut morphology and economic efficiency

The global aquaculture industry is increasingly seeking sustainable alternatives to fishmeal (FM) due to its high price and shortfall in supply. In this context, fermented aquatic weed meal (FAWM) could emerge as a viable plant protein source for aquafeed. Four isoproteic diets [30 % crude protein (CP)] were formulated, incorporating 50 % total protein from FAWM comprising fermented Azolla diet (D1), Pistia diet (D2), and Eichhornia diet (D3). The control diet (D0) did not contain FAWM. At the end of the 90 days feeding trial, their growth performance, whole-body proximate composition, gut microbial load, haemato-biochemical indices, liver and gut health, and economic efficiency were determined. Fish fed with D0 had significantly (p < 0.05) improved growth performance and feed utilization compared to other treatment groups. Meanwhile, the fish supplemented with D1 diet exhibited significantly (p < 0.05) higher final weight (g), specific growth rate (%/day), weight gain (%), total biomass (g), and protein efficiency ratio among the FAWM dietary groups. The D1 group also demonstrated the significantly (p < 0.05) highest whole-body CP (64.27 ± 0.40 %) and lower crude lipid (8.24 ± 0.28 %) compared to other test diets. The total bacteria (TB) and lactic acid bacteria (LAB) in the fish gut were found to be significantly (p < 0.05) higher in D1 group. Furthermore, most of the hemato-biochemical indices of fish were significantly (p < 0.05) affected by FAWM inclusions, with few exceptions. The histological findings indicated that amongst the FAWM groups, D1 fish exhibited improved intestinal health. Nonetheless, the gut of the control fish demonstrated substantially (p < 0.05) lower villi width and crypt depth than other treatments. The D1 and D2 diet groups had significantly improved liver health. Moreover, FAWM inclusion enhanced economic efficiency by considerably reducing farm feed cost (US$/kg) and increasing return on investment (%). In summary, dietary inclusion of fermented Azolla pinnata (D1) protein at 50 % in aquafeed promoted feed utilization, growth, health, and farm economics of Asian catfish fingerlings compared to other FAWM diets.

Enhancing concrete’s ballistic impact resistance using Alfa fiber

Natural fibers are gaining interest in recent years due to the increasing calls for eco-friendly materials production. Concrete reinforcement with Alfa fiber is attracting attention to take benefit of natural fiber advantages in the construction sector. The mechanical and physical characteristics of concrete reinforced with Alfa fibers have been primarily investigated. The current study seeks to analyse the ballistic protection performance of Alfa-reinforced concrete in response to the emerging need to withstand bullet impacts and protect occupants in light of the increasing armed hostilities. Eight formulations were selected for the concrete preparation by varying the Alfa fiber content from 0 to 100 kg/m³. After determining the mechanical characteristics of the concrete such as compressive, flexural and tensile strength, ballistic resistance to bullet impacts is determined by measuring the penetration depth, the crater area and the lost mass after impact in concrete slab 10 and 15 cm thick. The formulations of Alfa-reinforced concrete exhibited lower penetration depth, crater area, and lost mass compared to the reference concrete. Optimal values were achieved with 15 kg/m³ of fibers. A dependency between the ballistic response on the fibrous concrete and flexural strength of concrete is also remarked. In conclusion, the study demonstrates that incorporating Alfa fibers into concrete significantly enhances its ballistic resistance, with optimum performance observed at 15 kg/m³ of fiber inclusion. These findings underscore the potential of Alfa-reinforced concrete for use in military constructions and structures vulnerable to impact risks, paving the way for further research and practical applications in enhancing structural and occupant safety.

Diffusive and ebullitive methane emissions under tidal fluctuation and climate warming in a nutrient-rich subtropical estuary

Estuaries are important sources of methane (CH4) emissions under human activities and global warming. However, diffusive and ebullitive CH4 emissions in response to nutrients enrichments and tidal fluctuations remain largely constrained. Here, we investigated seasonal and monthly CH4 emissions in a nutrient-rich subtropical estuary and evaluated relative contribution of diffusion and ebullition CH4. Dissolved CH4 concentrations were in a range of 0.18–11.2 µmol L−1, corresponding to diffusive fluxes of 3.08–375 μg m−2 h−1 along the estuary. Diffusive CH4 fluxes were observed to decrease significantly from the upper to lower estuary and were higher in the cold than warm seasons. Turbidity, suspended particulate, NH4+, NO3−, DOC/NO3− and NO2− were the crucial factors affecting CH4 fluxes, collectedly explaining 43 % of CH4 emissions variability. Ebullitive and diffusive fluxes were significantly higher under ebbing than flooding tides, with an ebullition contribution of 82–98 %. Ecosystem-level temperature sensitivity and apparent activation energy of CH4 emissions were higher for ebullitive than diffusive pathways and were also higher under the ebbing than flooding tides, indicating that ebullitive pathway is high temperature and low water depth dependent. It is conservatively projected that CH4 emissions would increase by 17 ± 9 % and 40 ± 22 % under the 2 and 4 °C warming scenarios, respectively. These results suggest that enhanced CH4 emissions under human activities and climate warming can be refined in further CH4 projections and highlight the importance of estuaries in global carbon cycles and climate change.

Turbulence and sediment deposition in a channel with floating vegetation

Floating canopies of vegetation alter the vertical profile of flow velocity within and below canopies, which in turn impacts sediment deposition below canopies. This study explored the impacts of channel-average velocity (U0), vegetation density (a), and relative flow depth (hg/H, where hg is the height of the free flow region below the floating canopy and H is the entire flow depth) on the longitudinal distributions of near-bed turbulent kinetic energy (TKE) and sediment deposition in a channel with a floating vegetation canopy. Below a floating canopy, sediment deposition was correlated with the near-bed TKE. For the low-density and low-velocity cases, sediment deposition was spatially uniform across the entire channel because the near-bed TKE was lower than the critical value for sediment resuspension. As the vegetation density and channel-average velocity increased, the near-bed TKE increased and surpassed the critical TKE, which caused resuspension and reduced deposition below the canopy relative to the upstream reference. For the same vegetation density and channel-average velocity, a lower relative flow depth (hg/H) produced a higher below-canopy depth-averaged velocity and near-bed TKE, resulting in less deposition below the canopy. A model was proposed for predicting the longitudinal evolution of near-bed TKE based on the predicted flow velocity below the canopy throughout the channel. The longitudinal evolution of deposition below a floating canopy was predicted by combining the predicted near-bed TKE with the deposition probability. The predicted near-bed TKE and deposition matched laboratory measurements. Finally, the proposed model can be used to predict the longitudinal profile of deposition below a real Eichhornia crassipes canopy.

Tissue of origin detection for cancer tumor using low-depth cfDNA samples through combination of tumor-specific methylation atlas and genome-wide methylation density in graph convolutional neural networks.

224 Background: Cell free DNA (cfDNA)-based assays hold great potential in detecting early cancer signals yet determining the tissue-of-origin (TOO) for cancer signals remains a challenging task. Here, we investigated the contribution of a methylation atlas to TOO detection in low depth cfDNA samples. Methods: We constructed a tumor-specific methylation atlas (TSMA) using whole-genome bisulfite sequencing (WGBS) data from five types of tumor tissues (breast, colorectal, gastric, liver and lung cancer) and paired white blood cells (WBC). TSMA was used with a non-negative least square matrix factorization (NNLS) deconvolution algorithm to identify the abundance of tumor tissue types in a WGBS sample. We showed that TSMA worked well with tumor tissue but struggled with cfDNA samples due to the overwhelming amount of WBC-derived DNA. To construct a model for TOO, we adopted the multi-modal strategy and used as inputs the combination of deconvolution scores from TSMA with other features of cfDNA. Results: Our final model comprised of a graph convolutional neural network using deconvolution scores and genome-wide methylation density features, which achieved an accuracy of 69% in a held-out validation dataset of 239 low-depth cfDNA samples. Conclusions: In conclusion, we have demonstrated that our TSMA in combination with other cfDNA features can improve TOO detection in low-depth cfDNA samples.

Geophysical Mapping of Cemented Subsoils for Agricultural Development in Southern Peru

Cemented subsoils, commonly referred to as caliche, pose a regular challenge for agricultural development in arid and semi-arid regions like coastal southern Peru. These subsurface features restrict root penetration, limit water infiltration and hinder essential soil processes, ultimately reducing crop yields and agricultural productivity. Accurate and efficient mapping of caliche is important for optimizing land-use planning and implementing sustainable agricultural practices. This study presents the application of near-surface geophysical techniques for mapping caliche deposits in the context of agricultural development at the future Majes II site in the Arequipa region of southern Peru. Specifically, we employed high-frequency ground-penetrating radar (GPR) and frequency-domain electromagnetics (FDEM) at a testbed on the Majes II site to evaluate their ability to delineate the extent, thickness, and depth of caliche within the local geology. GPR offers high-resolution imaging, effectively capturing sharp contrasts between caliche and surrounding materials, providing detailed information on the thickness (approximately 0.4 m) and the depth (up to 1.5 m) of the caliche layers. FDEM provides valuable insights into the presence of caliche at a faster rate of data acquisition and processing, enabling rapid assessment of the extent of caliche deposits, although with the tradeoff of lower resolution and depth information. We demonstrate that these two geophysical methods can be used separately or in an integrated manner for collaborative interpretation at the Majes II site to inform land management decisions, including identifying areas with favorable conditions for crop production and implementing targeted interventions to mitigate the adverse effects of caliche on agricultural productivity.

Plant phenology modulates and undersown cover crops mitigate N2O emissions

Mitigation of N2O emissions, a potent greenhouse gas, remain challenging due to knowledge gaps in plant-mediated nitrogen (N) transformation pathways, which limits ability to identify optimal approaches for efficient N utilization. We set up mesocosms with barley, Italian ryegrass, and barley in combination with Italian ryegrass to assess role of cover crop in N2O emission mitigation. Soil emitted N2O was collected simultaneously from the pots with plants at three growth stages: namely, vegetative, canopy expansion, and grain filling. The gas sample N2O contents, N in microbial biomass (MBN), mineral N content, and phospholipid fatty acid (PLFA) analysis in soils were determined at the three growth stages. Cumulatively, highest N2O was emitted from soil under Italian ryegrass (0.056 mg N g−1 soil) followed by barley (0.0051 mg N g−1 soil) and the least under barley and Italian ryegrass combination (0.0014 mg N g−1 soil). The high emissions under Italian ryegrass occurred at vegetative stage due to high reactive N availability. Strong emissions were observed at canopy expansion stage under barley and were linked to access to the large mineral N proportion redistributed to the lower depth as depicted by highest MBN (0.025 mg N g−1 soil) and decreased extractable N (0.0068 mg N g−1 soil). The high emissions under barley correlated with high fungal/bacterial ratio, pointing towards a fungal role in the emissions. The least soil N2O emissions under barley and Italian ryegrass combination were accompanied by elimination of variations induced by the plant growth stages. Absence of 18:2ω6,9 fungal PLFA biomarker under barley and Italian ryegrass combination indicates a potential inhibition and corresponds with reduced N2O emissions. Together, these results broaden our understanding on how plant-soil interactions drives N2O emissions processes and improves our ability to identify optimal plant-based emission mitigation approaches.

Preoperative anatomical landmarks and longitudinal HeartMate 3 pump position in X-rays: Relevance for adverse events.

Left ventricular assist device (LVAD) malposition has been linked to hemocompatibility-related adverse events (HRAEs). This study aimed to identify preoperative anatomical landmarks and postoperative pump position, associated with HRAEs during LVAD support. Pre- and postoperative chest X-ray measures (≤14 days pre-implantation, first postoperative standing, 6, 12, 18, and 24 months post-implantation) were analyzed for their association with HRAEs over 24 months in 33 HeartMate 3 (HM3) patients (15.2% female, age 66 (9.5) years). HM3 patients with any HRAE showed significantly lower preoperative distances between left ventricle and thoracic outline (dLVT) (25.3 ± 10.2 mm vs. 40.3 ± 15.5 mm, p = 0.004). A ROC-derived cutoff dLVT ≤ 29.2 mm provided 85.7% sensitivity and 72.2% specificity predicting any HRAE during HM3 support (76.2% (>29.2 mm) vs. 16.7% (≤29.2 mm) freedom from HRAE, p < 0.001) and significant differences in cardiothoracic ratio (0.58 ± 0.04 vs. 0.62 ± 0.04, p = 0.045). Postoperative X-rays indicated lower pump depths in patients with ischemic strokes (9.1 ± 16.2 mm vs. 38.0 ± 18.5 mm, p = 0.007), reduced freedom from any neurological event (pump depth ≤ 28.7 mm: 45.5% vs. 94.1%, p = 0.004), and a significant correlation between pump depth and inflow cannula angle (r = 0.66, p < 0.001). Longitudinal changes were observed in heart-pump width (F(4,60) = 5.61, p < 0.001). Preoperative X-ray markers are associated with postoperative HRAE occurrence. Applying this knowledge in clinical practice may enhance risk stratification, guide therapy optimization, and improve HM3 recipient management.

Lacticaseibacillus rhamnosus inhibits the development of dental caries in rat caries model and in vitro

ObjectivesDental caries result from a microbial imbalance in the oral cavity. Probiotics ecologically modulate the oral microflora to prevent caries. This study evaluated the anti-cariogenic effects of two Lacticaseibacillus rhamnosus strains in vitro and in vivo to provide a more theoretical basis for its clinical applications in caries prevention. MethodsIn the study, cariogenic biofilms were grown with L. rhamnosus (LGG) or L. rhamnosus ATCC 7469 and analyzed. Quantitative real-time PCR (qPCR), Scanning Electron Microscope (SEM), and Confocal laser scanning microscope (CLSM) were used to detect the changes in the composition and architectures; cariogenic activity was measured by the lactic acid production and Transverse Microradiography (TMR). The effects of LGG on the 12 Sprague-Dawley rat caries model were assessed using Keyes scores and micro-CT analysis. Oral microbiome changes were evaluated through 16S rRNA gene high-throughput sequencing. ResultsL. rhamnosus can reduce cariogenic bacteria in biofilm by 14.7 % to 48.9 %, with LGG exhibiting more potent inhibitory effects. Both strains of L. rhamnosus can adhere to the surface of biofilms, reduce the extracellular polysaccharides (EPS) matrix, and loosen the biofilm structure. L. rhamnosus inhibited cariogenic activity by reducing the lactic acid production in biofilms. The bovine enamel blocks presented lower mineral loss values and lesion depth values in the group Core+L.rh and Core+LGG. LGG-ingested rats had significantly lower levels of moderate dentin lesions and higher mineral density than the control group. The 16 s rRNA gene sequencing revealed that LGG regulated the beta diversity of the oral microbial community in the rat dental caries model. ConclusionsThis study revealed the promising potential of L. rhamnosus, especially the LGG strain, in the ecological prevention of dental caries. Clinical SignificanceProbiotics may provide a strategy for preventing caries by regulating the oral microecological balance. The study revealed the promising anti-caries potential of the LGG probiotic strain in vivo and in vitro. It is expected that LGG could be used as an oral probiotic for the clinical prevention and treatment of caries.

Benchmarking pharmacogenomics genotyping tools: Performance analysis on short-read sequencing samples and depth-dependent evaluation.

Pharmacogenomics (PGx) investigates the influence of genetics on drug responses, enabling tailored treatments for personalized healthcare. This study assessed the accuracy of genotyping six genes using whole genome sequencing with four different computational tools and various sequencing depths. The effects of using different reference genomes (GRCh38 and GRCh37) and sequence aligners (BWA-MEM and Bowtie2) were also explored. The results showed generally minor variations in tool performance across most genes; however, more notable discrepancies were observed in the analysis of the complex CYP2D6 gene. Cyrius, a CYP2D6-specific tool, demonstrated the most robust performance, achieving the highest concordance rates for CYP2D6 in all instances, comparable to the consensus approach in most cases. There were rather small differences between the samples with 20× coverage depth and those with higher depth, but the decreased performance was more evident at lower depths, particularly at 5×. Additionally, variations in CYP2D6 results were observed when samples were aligned to different reference genomes using the same method, or to the same genome using different aligners, which led to reporting incorrect rare star alleles in several cases. These findings inform the selection of optimal PGx tools and methodologies as well as suggest that employing a consensus approach with two or more tools might be preferable for certain genes and tool combinations, especially at lower sequencing depths, to ensure accurate results. Additionally, we show how the upstream alignment can affect the performance of tools, an important factor to take into account.

Mitochondria-targeted sonodynamic modulation of neuroinflammation to protect against myocardial ischemia‒reperfusion injury

Sympathetic hyperactivation and inflammatory responses are the main causes of myocardial ischemia‒reperfusion (I/R) injury and myocardial I/R-related ventricular arrhythmias (VAs). Previous studies have demonstrated that light-emitting diodes (LEDs) could modulate post-I/R neuroinflammation, thus providing protection against myocardial I/R injury. Nevertheless, further applications of LEDs are constrained due to the low penetration depth (<1 cm) and potential phototoxicity. Low-intensity focused ultrasound (LIFU), an emerging noninvasive neuromodulation strategy with deeper penetration depth (∼10 cm), has been confirmed to modulate sympathetic nerve activity and inflammatory responses. Sonodynamic therapy (SDT), which combines LIFU with sonosensitizers, confers additional advantages, including superior therapeutic efficacy, precise localization of neuronal modulation and negligible side effects. Herein, LIFU and SDT were introduced to modulate post-myocardial I/R neuroinflammation to protect against myocardial I/R injury. The results indicated that LIFU and SDT inhibited sympathetic neural activity, suppressed the activation of astrocytes and microglia, and promoted microglial polarization towards the M2 phenotype, thereby attenuating myocardial I/R injury and preventing I/R-related malignant VAs. These insights suggest that LIFU and SDT inspire a noninvasive and efficient neuroinflammatory modulation strategy with great clinical translation potential thus benefiting more patients with myocardial I/R in the future. Statement of significanceMyocardial ischemia-reperfusion (I/R) may cause I/R injury and I/R-induced ventricular arrhythmias. Sympathetic hyperactivation and inflammatory response play an adverse effect in myocardial I/R injury. Previous studies have shown that light emitting diode (LED) can regulate I/R-induced neuroinflammation, thus playing a myocardial protective role. However, due to the low penetration depth and potential phototoxicity of LED, it is difficult to achieve clinical translation. Herein, we introduced sonodynamic modulation of neuroinflammation to protect against myocardial I/R injury, based on mitochondria-targeted nanosonosensitizers (CCNU980 NPs). We demonstrated that sonodynamic modulation could promote microglial autophagy, thereby preventing myocardial I/R injury and I/R-induced ventricular arrhythmias. This is the first example of sonodynamic modulation of myocardial I/R-induced neuroinflammation, providing a novel strategy for clinical translation.

No Field Evidence of Grass Fuel Structure effects on Postfire Tree Mortality in Juniperus virginiana

Prescribed fires are an important management tool for containing woody plant encroachment in rangeland ecosystems. Grasses are the dominant fuel type in rangelands. Past work has shown that grass canopy architecture, which varies among grass species, can influence flammability. Whether variation in grass fuel structure can influence postfire plant responses has not yet been tested. To bridge this gap, we set up field burning experiments with different fuel treatments and examined postfire mortality of Juniperus virginiana L. in a tallgrass prairie in southwestern Missouri. We sampled 60 trees and measured tree height and diameter at breast height before the fire. Fuels surrounding each tree were manipulated to vary independently in both fuel load and fuel structure. Flame temperatures were measured during the fire, and both stem and canopy injuries were evaluated 1 d after the fire. We surveyed tree mortality 7 mo after the fire. We found no effects of either fuel load or fuel structure on postfire mortality or on canopy injury in J. virginiana. Canopy injury was a critical fire severity measurement determining postfire mortality in J. virginiana, and taller trees are more fire resilient. Despite laboratory-observed fuel structure effects on flammability, this study finds no evidence for the importance of grass fuel load and canopy architecture in influencing postfire tree response. This result might arise from the low crown depth and low canopy water content of J. virginiana, which can promote canopy fire and result in a high mortality rate across fuel treatments. Notwithstanding the negative results, testing laboratory-based findings in field settings is important for further examining laboratory observations and upscaling individual-level processes to ecosystems to help identify the key ecological processes determining population dynamics and community assembly. Our study also suggests that prescribed fire is an effective tool to remove encroaching J. virginiana in tallgrass prairies at an early stage.

Atomistic insight of deformation mechanisms and mechanical characteristics of nano-scale silver (100) using nanoindentation

This work aims to utilize the widely adopted nanoindentation technique to explore the deformation behavior and mechanical characteristics of nanocrystalline silver (Ag) materials. Molecular Dynamics simulations were conducted to understand the material behavior at the nanometer scale. In this simulation, the effect of indentation velocity and indentation depth on defect formation and hardness during nanoindentation of Ag specimens was analyzed and discussed in detail. The results show that the deformation behavior of the Ag specimen was affected by different indentation velocities. These phenomena were confirmed by analyzing the load-displacement curve, which indicates elastic deformation by the appearance of a linear load-displacement at low indentation velocities. In contrast, plastic deformation was found by the presence of intensified serration in the load-displacement relationship at higher indentation velocities. In these cases, the hardness showed little variation when the indenter velocity was increased from 5 m/s to 10 m/s, whereas a significant increase in hardness was observed when the indentation velocity was further increased to 20 m/s. Importantly, the progression of load-displacement curves during nanoindentation demonstrates the occurrence of minor and major load drops, corresponding to an increase in indentation depth from 0.5 nm to 2 nm respectively. These findings were elucidated through dislocation extraction analysis (DXA), which revealed that a minor load drop at low indentation depths is attributed to the nucleation of an amorphous structure, while a major load drop at higher indentation depths is associated with the expansion of dislocation nucleation. Consequently, the hardness increased significantly as the indentation depth increased. This research, provides insight into atomistic investigation and offers guidelines for designing materials with excellent mechanical properties using nanoindentation.

Near-Infrared Light-Mediated Antibacterial Photodynamic Therapy Based on Erythrosine-Functionalized Mesoporous Silica-Coated Upconversion Nanoplatform.

Infectious diseases caused by bacteria pose a serious threat to public health, and more worryingly, the unregulated application of antibiotics accelerates the emergence of bacterial resistance, presenting a major challenge to the effective treatment of infectious diseases caused by bacteria. Therefore, there is an urgent necessity to develop efficient and safe antimicrobial systems. Photodynamic therapy (PDT) is an attractive therapeutic approach that does not induce bacterial resistance. However, the clinical application of PDT has been limited by several factors, including the lower tissue penetration depth of photoactivation under visible light irradiation and the uncertain biosafety of photosensitizers (PS). This work presents an near infrared (NIR)-triggered core-shell upconversion nanoparticle-based PDT system composed of mesoporous silica-coated lanthanide-doped upconversion nanoparticles loaded with the photosensitizer erythrosine (UCSE). Upon NIR-triggering, erythrosine generates highly efficient reactive oxygen species that disrupt the cell membranes of Staphylococcus aureus and Escherichia coli, exhibiting a potent photodynamic antimicrobial effect. It is worth noting that the UCSE also exhibits excellent biosafety. In conclusion, we present an efficient NIR-triggered nanoantimicrobial system with excellent antimicrobial capacity and biosafety, which is a new therapeutic strategy for the control of bacterial infectious diseases.

Evaluation of the fiber optic curvature sensor design parameters for new measurement approach application

In this paper, the influence of sensitive zone design parameters on the performance of fiber optic curvature sensor (FOCS) with sensitive zone made of V-grooves is experimentally investigated. A new insight into FOCS operation is given by introducing optical mode converter and CCD sensor into experimental setup, thus enabling mode-dependent analysis of V-grooves depth and spacing influence on sensor sensitivity and providing increased measurement reliability. Based on experimental analysis conducted in this work it is shown that FOCS intensity sensitivity increases approximately linear with sensitive zone depth and length, with highest sensitivity obtained for low order modes. According to this mode-dependent analysis, a new and more reliable measurement approach based on measurement of changes in the width of the output light distribution, rather than intensity, is proposed and tested. FOCS FWHM sensitivity increases approximately linear with depth and length of the sensitive zone, with higher sensitivity obtained in case of convex deformation. However, better linearity and balanced FWHM response is obtained for lower depths and shorter lengths of sensitive zone.

Diverse and ecologically unique mesophotic coral ecosystems in the central Indian Ocean

Research on Mesophotic Coral Ecosystems (MCEs; 30–150 m) has grown exponentially in the last few decades, highlighting their rich diversity and extensive distribution. However, they are still largely underexplored compared to shallow-water coral reefs and frequently remain under-protected and under-represented in marine spatial planning. One reason for the imbalance between the high ecological value of MCEs and the limited levels of protection may be that baseline data on MCEs are largely missing to date, yet are crucial to provide evidence-based information for management actions. Here, we present data on the alpha and beta diversity of the benthic communities within MCEs in the Chagos Archipelago, Indian Ocean. Using imagery collected from Remotely Operated Vehicle surveys, benthic invertebrate megafauna were surveyed along the entire depth gradient from shallow to lower mesophotic depths (15–160 m). The diversity of the benthic communities decreased with increasing depth, from shallow water to the lower mesophotic zone. Nevertheless, the deepest parts of MCEs in the Archipelago displayed higher species richness values than several other shallow subtropical regions. In addition, the benthic communities showed high dissimilarity along the depth gradient, indicating that the key driver of community composition change with depth is species turnover (species replacement), revealing the uniqueness of MCEs. This study presents novel findings on MCEs in the central Indian Ocean, demonstrating that they host a high and unique benthic diversity, and highlighting the need to protect these ecosystems to preserve the overall biodiversity of coral reefs.

The Frictional‐Viscous Transition in Experimentally Deformed Granitoid Fault Gouge

AbstractIn crustal faults dominated by granitoid gouges, the frictional‐viscous transition marks a significant change in strength constraining the lower depth limit of the seismogenic zone. Dissolution‐precipitation creep (DPC) may play an important role in initiating this transition, especially within polymineralic materials. Yet, it remains unclear to what extent DPC contributes to the weakening of granitoid gouge materials at the transition. Here we conducted sliding experiments on wet granitoid gouges to large displacement (15 mm), at an effective normal stress and pore fluid pressure of 100 MPa, at temperatures of 20–650°C, and at sliding velocities of 0.1–100 μm/s, which are relevant for earthquake nucleation. Gouge shear strengths were generally ∼75 MPa even at temperatures up to 650°C and at velocities &gt;1 μm/s. At velocities ≤1 μm/s, strengths decreased at temperatures ≥450°C, reaching a minimum of 37 MPa at the highest temperature and lowest velocity condition. Microstructural observations showed that, as the gouges weakened, the strain localized into thin, dense, and ultrafine‐grained (≤1 μm) principal slip zones, where nanopores were located along grain contacts and contained minute biotite‐quartz‐feldspar precipitates. The stress sensitivity exponent n decreased from a large number at 20°C to ∼2.2 at 650°C at the lowest velocities. These findings suggest that high temperature, slow velocity and small grain sizes promote DPC‐accommodated granular flow over cataclastic frictional granular flow, leading to the observed weakening and strain localization. Field observations together with extrapolation suggest that DPC‐induced weakening occurs at depths of 7–20 km depending on geothermal gradient.

Effect of MWCNTs on static, dynamic, and wear performance of Vacuum Assisted Resin Infusion Molding (VARIM) processed glass fabric/epoxy polymer composites

In the present work, MWCNTs were used in wt.% of 0.075, 0.15, and 0.3 in epoxy resin, and then glass fabric/epoxy polymer (GF/EP) composites were fabricated using VARIM setup. The mechanical and wear properties of GF/EP composites were compared with and without the addition of MWCNTs. Maximum improvement in tensile and flexural performance were seen in GF/EP composites at 0.15 weight percent MWCNT addition when compared to a neat GF/EP one, based on static testing such as tensile and flexural tests. High-cycle fatigue test results show a relatively higher cycle count with 0.15 wt.% MWCNTs addition in the GF/EP composite compared to the GF/EP without the addition of MWCNTs. The wear performance also improved with a lower friction coefficient as well as a lower track depth and width for MWCNTs with added GF/EP than plain GF/EP composite. Therefore, in addition to decreasing surface softening and improving the wear performance of glass fabric epoxy composites, MWCNTs (0.15 weight percent) increased the interfacial adhesion at the fiber/matrix interface. This study establishes an optimum ratio of 0.15 wt.% of MWCNTs addition in glass fabric/epoxy polymer composites for enhancement in their mechanical and wear performance.

Impact of flexible vegetation on hydraulic characteristics in compound channels with converging floodplains

ABSTRACT Floodplains in natural rivers are curved and narrow, rather than following a straight path and the presence of vegetation and varying depth of flow affects the flow parameters of the channels. The purpose of this study is to investigate the effects of the convergence of the channel and relative depth of flow on the depth-averaged velocity (DAV) and boundary shear stress (BSS) of a converging compound channel. The effect of vegetation on DAV and BSS was also deliberated. To examine the effect of vegetation, synthetic grass 8 cm in height was used as a flexible vegetation to depict the natural conditions of the rivers. The DAV was calculated using an acoustic Doppler velocimeter (ADV) and the measurement of BSS was done using the Preston tube technique. After analysing the data, it was revealed that as the convergence of floodplains increases, maximum DAV increases. The presence of vegetation reduces the velocity specifically at lower flow depths. BSS increases with the convergence as well as vegetation. This research provides valuable data and results which will be helpful in better flood management, river restoration, and designing effective hydraulic structures.