A 60 days feeding trial was conducted to study the stress mitigation and hence growth augmenting effect of dietary l-tryptophan during high density group stress in Cirrhinus mrigala fingerlings. Four hundred eighty fingerlings were distributed into eight experimental groups. Each group either of low density group (10 fishes/75 L water) or higher density group (30 fishes/75 L water) was fed with a diet containing either 0, 0.68, 1.36 or 2.72% l-tryptophan in the diet, thus eight experimental groups viz. low density control (LC) (basal feed + 0% l-tryptophan); LT1 (basalfeed + 0.68% l-tryptophan); LT2 (basalfeed + 1.36% l-tryptophan); LT3 (basalfeed + 2.72% l-tryptophan) high density control (HC) (basal feed + 0% l-tryptophan); HT1 (basalfeed + 0.68% l-tryptophan); HT2 (basalfeed + 1.36% l-tryptophan); and HT3 (basalfeed + 2.72% l-tryptophan) were fed at 3% of the body weight with isonitrogenous (34.33 ± 0.23 to 35.81 ± 0.18 CP%) and isocaloric (423.49 ± 1.76 to 425.85 ± 0.31 K.Cal/100 g) purified diets. The possible role of dietary l-tryptophan on stress mitigation was assessed in terms of blood glucose, plasma cortisol, lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alanine amino transferase (ALT), aspartate amino transferase (AST), acetyl choline esterase (AChE) assays, whereas growth was evaluated in terms of weight gain %, specific growth (SGR) and protein efficiency ratio (PER). The LDH, MDH, ALT, AST activities were found to be significantly higher ( p < 0.05) in the control groups and decreasing trend of LDH, MDH, ALT and AST activities were observed with the increasing level of dietary l-tryptophan. However, high density group exhibited significantly higher value ( p < 0.05) for the above parameters than their low density counterpart at similar level of l-tryptophan in the diet. In contrast to above parameters, AChE activity exhibited a reverse trend. In both the stocking densities, l-tryptophan supplemented groups found to have higher ( p < 0.05) growth, SGR and PER. The Plasma cortisol level was found to be significantly higher in the high density group than its low density counterpart. However, gradual supplementation of l-tryptophan in diet significantly reduced the cortisol level both in high density ( Y = − 26.33 x + 219.53, r 2 = 0.95) and low density group ( Y = − 27.57 x + 169.28, r 2 = 0.92). Similar trend was also found in blood glucose. Hence, dietary supplementation of l-tryptophan at a minimum level of 1.36% concomitantly reduced the stress in C. mrigala fingerlings. Though 2.72% dietary tryptophan also reduces the stress but 1.36% level appears to be cost effective.
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