Low Yield Research Articles

Grafting onto Tomato Rootstocks Improves Outcomes for Dry-farmed Tomato

Vegetable grafting can mitigate the negative effects of drought on crop production. Dry farming, which is the production of crops without irrigation during a dry growing season, can result in lower yields, smaller fruit, and a higher incidence of blossom-end rot (BER), which is a physiological disorder associated with drought stress. To determine the effects of grafting on yield and fruit quality of dry-farmed tomato (Solanum lycopersicum), three years of trials were conducted using different scion–rootstock combinations and ungrafted controls. In 2020, grafting onto rootstocks ‘DRO141TX’ and ‘Fortamino’ resulted in greater total yield and average fruit weight and a lower BER incidence for dry-farmed tomato than grafting onto the rootstock ‘Shincheonggang’ or using ungrafted plants. In 2021, grafting onto the rootstock ‘DRO141TX’ again increased yields and average fruit weight and decreased BER incidence when compared with ungrafted plants (‘Fortamino’ was not tested). Interactions were detected between different scion–rootstock combinations in terms of the degree of reduction of necrotic BER (BER resulting in a large, sunken, grey or black spot, making the fruit unmarketable) when grafted onto ‘DRO141TX’, with the scion ‘Azoychka’ having a 69% reduction in necrotic BER and the scion ‘Astrakhanskie’ having a 93% reduction in necrotic BER. In 2022, an interaction was detected between the rootstocks and scions in terms of their effect on large fruit (>0.33 lb) yield, with ‘BHN 871’ grafted onto ‘Fortamino’ producing the highest yields of large fruit and ‘Big Beef’ grafted onto ‘Fortamino’ producing the lowest yields. Overall, grafting onto the rootstocks ‘DRO141TX’ or ‘Fortamino’ improved diverse dry-farmed tomato outcomes in the Willamette Valley of Oregon, USA.

Systems metabolic engineering of Corynebacterium glutamicum to assimilate formic acid for biomass accumulation and succinic acid production

Formate as an ideal mediator between the physicochemical and biological realms can be obtained from electrochemical reduction of CO2 and used to produce bio-chemicals. Yet, limitations arise when employing natural formate-utilizing microorganisms due to restricted product range and low biomass yield. This study presents a breakthrough: engineered Corynebacterium glutamicum strains (L2-L4) through modular engineering. L2 incorporates the formate-tetrahydrofolate cycle and reverse glycine cleavage pathway, L3 enhances NAD(P)H regeneration, and L4 reinforces metabolic flux. Metabolic modeling elucidates C1 assimilation, guiding strain optimization for co-fermentation of formate and glucose. Strain L4 achieves an OD600 of 0.5 and produces 0.6 g/L succinic acid. 13C-labeled formate confirms C1 assimilation, and further laboratory evolution yields 1.3 g/L succinic acid. This study showcases a successful model for biologically assimilating formate in C. glutamicum that could be applied in C1-based biotechnological production, ultimately forming a formate-based bioeconomy.

Integrated Management of Rhizome Rot and Wilt Disease Complex of Ginger (Zingiber officinale) – A Review

This review article focuses on integrated management options for ginger diseases, especially rhizome rot and wilt. We compiled the results of several strategies, including chemical treatments, cultural practices, soil solarization, organic additions, and biological management for rhizome rot and wilt disease complex of ginger. Effective approaches includes (i) raise the temperature by soil solarization, (ii) additions of soil amendments like wood sawdust, oil cakes, neem cakes that prevent soil pathogen and simultaneously improve soil health, (iii) cultural techniques such as soil selection, crop rotation and intercropping, (iv) biological control using Trichoderma species is another useful method to lower infestation and increase ginger yield, and (v) use of chemical control measures such as Ridomil MZ, Metalaxyl, and Streptomycin. The above-listed aspects can be integrated to develop integrated management strategies that aid in developing a treatment strategy for management for rhizome rot and wilt disease complex of ginger.

Ductile Fracture Prediction Framework of Low Yield Point Steel LYP225 under Monotonic Loading: Experiment and Simulation

Ductile Fracture Prediction Framework of Low Yield Point Steel LYP225 under Monotonic Loading: Experiment and Simulation

A Comparison Between Modified-umbrella and High-wire Trellising Systems in a Low-profile Greenhouse for Hydroponic Beit Alpha Cucumber

The production of Beit Alpha cucumber (Cucumis sativus) in hydroponic systems has increased in popularity since the early 2000s, along with the use of high-wire trellising systems. Some farmers claim the high-wire trellising systems, also known as drop-and-lean trellising, result in a more consistent weekly yield than umbrella or modified-umbrella systems. This study compared the high-wire and modified-umbrella trellising systems both using a 7 ft top wire and 4 plants/m2 plant density. The fruit weight and number of fruit per plant were significantly greater using the modified-umbrella trellising system, with the number of fruit being about twice as high as the high-wire trellising system. Consistency of yields was also measured for both systems and found to peak at ∼5 to 7 weeks after the start of harvest using both trellising systems. However, peak yields using the modified-umbrella trellising system followed a quadratic curve, implying that the high-wire trellising system results in more consistent yields. Differences in yield and harvest consistency were likely related to light penetration of the plant canopy. Growers using low-profile greenhouses can expect lower yields and more consistent harvests using the high-wire trellising system. Yields may be improved using a higher plant density. Alternatively, succession planting on a 5- to 7-week interval can improve harvest consistency using a modified-umbrella trellising system.

Upconverting Nanoparticles Coated with Light-Breakable Mesoporous Silica for NIR-Triggered Release of Hydrophobic Molecules.

Upconverting nanoparticles (UCNPs) doped with Yb3+ and Tm3+ are near-infrared (NIR) to ultraviolet (UV) transducers that can be used for NIR-controlled drug delivery. However, due to the low quantum yield of upconversion, high laser powers and long irradiation times are required to trigger this drug release. In this work, we report the one-step synthesis of a nanocomposite consisting of a LiYbF4:Tm3+@LiYF4 UCNP coated with mesoporous UV-breakable organosilica shells of various thicknesses. We demonstrate that a thin shell accelerates the breakage of the shell at 1 W/cm2 NIR light exposure, a laser power up to 9 times lower than that of conventional systems. When the mesopores are loaded with hydrophobic vitamin D3 precursor 7-dehydrocholesterol (7-DH), shell breakage results in subsequent cargo release. Its minimal toxicity in HeLa cells and successful internalization into the cell cytoplasm demonstrate its biocompatibility and potential application in biological systems. The tunability of this system due to its simple, one-step synthesis process and its ability to operate at low laser powers opens up avenues in UCNP-powered NIR-triggered drug delivery toward a more scalable, flexible, and ultimately translational option.

Antimalarial resistance risk in Mozambique detected by a novel quadruplex droplet digital PCR assay.

While the Plasmodium falciparum malaria parasite continues to cause severe disease globally, Mozambique is disproportionally represented in malaria case totals. Acquisition of copy number variations (CNVs) in the parasite genome contributes to antimalarial drug resistance through overexpression of drug targets. Of interest, piperaquine resistance is associated with plasmepsin 2 and 3 CNVs (pfpmp2 and pfpmp3, respectively), while CNVs in the multidrug efflux pump, multidrug resistance-1 (pfmdr1), increase resistance to amodiaquine and lumefantrine. These antimalarials are partner drugs in artemisinin combination therapies (ACTs) and therefore, CNV detection with accurate and efficient tools is necessary to track ACT resistance risk. Here, we evaluated ~300 clinically derived samples collected from three sites in Mozambique for resistance-associated CNVs. We developed a novel, medium-throughput, quadruplex droplet digital PCR (ddPCR) assay to simultaneously quantify the copy number of pfpmp3, pfpmp2, and pfmdr1 loci in these clinical samples. By using DNA from laboratory parasite lines, we show that this nanodroplet-based method is capable of detecting picogram levels of parasite DNA, which facilitates its application for low yield and human host-contaminated clinical surveillance samples. Following ddPCR and the application of quality control standards, we detected CNVs in 13 of 229 high-quality samples (prevalence of 5.7%). Overall, our study revealed a low number of resistance CNVs present in the parasite population across all three collection sites, including various combinations of pfmdr1, pfpmp2, and pfpmp3 CNVs. The potential for future ACT resistance across Mozambique emphasizes the need for continued molecular surveillance across the region.

The role and mechanism of engineered nanovesicles derived from hair follicle mesenchymal stem cells in the treatment of UVB-induced skin photoaging.

Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are effective in the treatment of skin photoaging; however, their low yield and functional decline with passage progression limit their clinical application. Cell-derived nanovesicles (CNVs) are potential alternatives that can address the limitations of EVs derived from MSCs and are conducive to clinical transformations. Hair follicle mesenchymal stem cells (HFMSCs), a type of MSCs, have demonstrated the function of repairing skin tissues; nevertheless, the efficacy of CNVs from HFMSCs (HFMSC-CNVs) in the treatment of skin photoaging remains unclear. Therefore, ultraviolet radiation B (UVB)-induced photoaging nude mice and human dermal fibroblasts (HDFs) were used as experimental models to investigate the therapeutic effects of HFMSC-CNVs in photoaging models. HFMSC-CNVs were successfully prepared using the mechanical extrusion method. UVB-induced nude mice and HDFs were used as experimental models of photoaging. Multiple approaches, including hematoxylin-eosin and Masson staining, immunohistochemistry, immunofluorescence, detection of reactive oxygen species (ROS), flow cytometry, western blotting, and other experimental methods, were combined to investigate the possible effects and mechanisms of HFMSC-CNVs in the treatment of skin photoaging. In the nude mouse model of skin photoaging, treatment with HFMSC-CNVs reduced UVB-induced skin wrinkles (p < 0.05) and subcutaneous capillary dilation, alleviated epidermis thickening (p < 0.001), and dermal thinning (p < 0.001). Furthermore, HFMSC-CNVs upregulated proliferating cell nuclear antigen (PCNA) expression (p < 0.05) and decreased the levels of ROS, β-galactosidase (β-Gal), and CD86 (p < 0.01). Invitro experiments, treatment with HFMSC-CNVs enhanced the cellular activity of UVB-exposed HDFs (p < 0.05), and reduced ROS levels and the percentage of senescent cells (p < 0.001), and alleviated cell cycle arrest (p < 0.001). HFMSC-CNVs upregulated the expression of Collagen I (Col I), SMAD2/3, transforming growth factor beta (TGF-β), catalase (CAT), glutathione peroxidase-1 (GPX-1), and superoxide dismutase-1 (SOD-1) (p < 0.05) and downregulated the expression of cycle suppressor protein (p53), cell cycle suppressor protein (p21), and matrix metalloproteinase 3 (MMP3) (p < 0.05). Conclusively, the anti-photoaging properties of HFMSC-CNVs were confirmed both invivo and invitro. HFMSC-CNVs exert anti-photoaging effects by alleviating cell cycle arrest, decreasing cellular senescence and macrophage infiltration, promoting cell proliferation and extracellular matrix (ECM) production, and reducing oxidative stress by increasing the activity of antioxidant enzymes.

MORN motif-containing protein OsMORN1 and OsMORN2 are crucial for rice pollen viability and cold tolerance.

The pollen viability directly affects the pollination process and the ultimate grain yield of rice. Here, we identified that the MORN motif-containing proteins, OsMORN1 and OsMORN2, had a crucial role in maintaining pollen fertility. Compared with the wild type (WT), the pollen viability of the osmorn1 and osmorn2 mutants was reduced, and pollen germination was abnormal, resulting in significantly lower spikelet fertility, seed-setting rate, and grain yield per plant. Further investigation revealed that OsMORN1 was localized to the Golgi apparatus and lipid droplets. Lipids associated with pollen viability underwent alterations in osmorn mutants, such as the diacylglyceride (18:3_18:3) was 5.1-fold higher and digalactosyldiacylglycerol (18:2_18:2) was 5.2-fold lower in osmorn1, while the triacylglycerol (TG) (16:0_18:2_18:3) was 8.3-fold higher and TG (16:0_18:1_18:3) was 8.5-fold lower in osmorn2 than those in WT. Furthermore, the OsMORN1/2 was found to be associated with rice cold tolerance, as osmorn1 and osmorn2 mutants were more sensitive to chilling stress than WT. The mutants displayed increased hydrogen peroxide accumulation, reduced antioxidant enzyme activities, elevated malondialdehyde content, and a significantly decreased seedling survival rate. Lipidomics analysis revealed distinct alterations in lipids under low temperature, highlighting significant changes in TG (18:2_18:3_18:3) and TG (18:4_18:2_18:2) in osmorn1, TG (16:0_18:2_18:2) and PI (17:2_18:3) in osmorn2 compared to the WT. Therefore, it suggested that OsMORN1 and OsMORN2 regulate both pollen viability and cold tolerance through maintaining lipid homeostasis.

Sustainable Enzymatic Production of Omega-3 Oil from Squid Viscera

Fish oils are widely consumed around the world to increase omega-3 fatty acid intake. Due to negative impacts on marine resources and ecosystems from an increasing demand for fish, alternative sustainable sources are under investigation. Squid viscera contains up to 10% oil by mass and is available as a byproduct from squid processing. Squid viscera oil is a source of EPA and DHA and contains the xanthophyll carotenoid astaxanthin, known for its significant anticancer, antioxidant, antidiabetic, and cardiovascular properties. In the raw form, squid viscera oil has a high free fatty acid (FFA) content, so conventional alkaline refining results in low yield and loss of astaxanthin. As a higher-yielding alternative, the current study optimized lipase-catalyzed glycerolysis of squid viscera oil to convert FFA into acylglycerol using a custom-built one-liter immobilized enzyme reactor. To monitor the reaction progress and assess its impact on the oil, we analyzed lipid classes, fatty acid composition and astaxanthin levels. Under optimized conditions, FFA was reduced from 40% to 2.7% in 10 h and 1.7% in 24 h, with no significant effect on EPA and DHA levels, and astaxanthin being retained. Squid viscera presents a safe and sustainable additional source of marine-derived EPA and DHA oil.

Effect of Milk Sources on Whey Protein and Fractions of Casein

Aim: Milk from Cow, Buffalo and goat was used for the study of isolation and fractionation of casein and whey proteins. Buffalo and goat milk were subjected to physicochemical attribute studies and a detailed protein profile namely total protein, casein fractions α-, β- and κ casein and whey protein were separated by urea fractionation method. The aim of this study was to compare various casein fractions isolated from cows, Buffalo and Goat milk. The method of separating casein into its fractions is based on the solubility of the individual components in urea the solution. The separation of casein fractions was carried out in dissolving molar concentration of urea and the yield of fractions and total protein percentages were calculated. Buffalo and goat milk have comparatively higher total protein and whey protein content as compared to cow milk. Maximum whey proteins found in goat milk (20.58%) whereas cow milk had the lowest whey protein (19.29%). Among casein fractions, α fraction (16.64%) was found maximum in Buffalo milk followed by cow (14.92%) and goat milk (5.42%). The goat milk showed the highest level of β casein (17.81%) whereas, the lowest concentration of β casein was found in cow milk (9.38%). Study Design: A significant contribution to the total milk production of India comes from buffalo milk and goat milk. Despite that, Buffalo milk and goat milk are not being utilized for many products because of difference in composition of different species milk and their inherent problems associated in the production of products. Place and Duration of Study: Karnataka Veterinary Animal and Fisheries Sciences University (KVAFSU), Dairy Science College, Hebbal, Bangalore Karnataka, India Materials: The Goat milk samples were collected from Sinchana Goat and Sheep farm, Marenahalli village (Bengaluru Rural Dist) and Buffalo milk was obtained from Country Delight Pvt. Ltd., J. P. Nagar, Bengaluru, Karnataka. Cow milk used in this investigation was collected from SEDP, Dairy Science College, Hebbal, Bengalore.. All the glassware used was soaked in chromic acid solution, repeatedly washed with water, rinsed with distilled water and dried before use. For microbiological analysis dried test tubes, conical flasks, pipettes were cotton plugged and sterilized in a hot air oven. The chemicals and reagents used in this study were mainly of analytical grade procured from Prince Laboratory Company Pvt. Limited, Bangalore. The protein molecular weight markers used for the electrophoretic study were procured from Bangalore Genei Pvt. Ltd. Methodology: Standard urea fractionation method adopted by Hipp et al was used for isolation and fractionation of caseins. Skim milk was prepared by subjecting whole milk for centrifugation, the skim milk obtained was acidified using 1 % hydrochloric acid at 200 C to obtain casein precipitate. Obtained whole casein was dissolved in 3.3 M urea at pH 7.5 and adjusted to pH 4.6 which precipitates the bulk of α casein and leaving k-caseins into soluble filtrates. Again the supernatant was adjusted to pH 4.9 diluted to 1.0 M urea and warmed to 300 C precipitating the β-caeisn. Further, addition of ammonium sulphate was used to isolate k-casein from the precipitate. Results: The sources of protein had a significant influence on the total casein, casein fractions and whey protein content of different species. The highest quantity of total caseins (34.30g/l) and whey protein (8.87 g/l) were observed in buffalo milk followed by cow (28.52 g/l) and lowest was observed in goat milk (28.45 g/l). Total protein, casein and whey protein contents were greatly affected by the source of milk. Conclusion: The significant effect of source of milk on total protein, casein, its fractions and their yield was observed. highest yield of casein (54.31%) was observed in cow milk than buffalo milk (48.95 %) and lower yield was found in goat milk. Amongst the three species, highest per cent of β casein was noted in goat milk (54.05 %) followed by buffalo (36.03%) and cow milk (34.14%). Recommendation: Rasagulla will be consumed universally by all age groups globally. Thus, the consumption of Rasagulla helps in providing overall nutritional requirements. However, the study has been conducted on a pilot scale. Moreover, commercial production may necessitate huge cost on enzymatic modification of proteins and separation peptides for value addition.

Impaired SHH responsiveness of induced pluripotent stem cell-derived floor plate cells carrying the LRRK2 I1371V mutation contributes to the ontogenic origin of lower dopaminergic-neuron yield.

Lower population of dopaminergic (DA)-neurons is known to increase susceptibility to PD, and our earlier study showed a lower yield of DA-neurons in LRRK2-I1371V mutation-carrying PD patient-iPSCs. While the role of SHH in DA-neurogenesis of Floor-Plate Cells (FPCs) is known, effect of LRRK2 mutations on SHH-responsiveness of FPCs impacting DA-neuronal yield has not been studied. We investigated SHH-responsiveness of FPCs derived from LRRK2-I1371V PD patient-derived iPSCs with regard to the expression of SHH receptors Patched1 (Ptch1) and Smoothened (Smo), in conjunction with nuclear Gli1 expression, intracellular Ca2+ rise, and cytosolic cAMP levels upon SHH induction. Additionally, we examined the mechanistic link with LRRK2-I1371V gain-of-function by assessing membrane-fluidity and Rab8A & Rab10 phosphorylation in SH-SY5Y cells and healthy control (HC)-FPCs overexpressing LRRK2-I1371V as well as FPCs. While total expression of Ptch1 and Smo was comparable, receptor expression on cell-surface was significantly lower in LRRK2-I1371V FPCs than in HC, with distinctly lower nuclear-expression of the downstream transcription factor Gli1. HC-FPCs transfected with LRRK2 I1371V exhibited a similarly reduced cell-surface expression of Ptch1 and Smo. Intracellular Ca2+ response was significantly lower with corresponding elevated cAMP levels in LRRK2-I1371V FPCs compared to HC upon SHH-stimulation. Both LRRK2-I1371V mutant FPCs and LRRK2-I1371V transfected SH-SY5Y and HC-FPCs further exhibited higher autophosphorylation of phospho LRRK2 (pLRRK2) serine1292 and serine935, as well as substrate phosphorylation of Rab8A & Rab10. Concurrent increase in membrane fluidity, accompanied by a decrease in membrane cholesterol, and lower expression of lipid raft marker Caveolin1 were also observed in them. These findings suggest that impaired SHH-responsiveness of LRRK2-I1371V PD FPCs indeed leads to lower yield of DA-neurons during ontogeny. Reduced cell-surface expression of SHH receptors is influenced by alteration in membrane fluidity owing to the increased substrate phosphorylation of Rab8A and reduced membrane protein trafficking due to pRab10, both results of the LRRK2-I1371V mutation.

Modulation of the photobehavior of gefitinib and its phenolic metabolites by human transport proteins

The photobiological damage that certain drugs or their metabolites can photosensitize in proteins is generally associated with the nature of the excited species that are generated upon interaction with UVA light. In this regard, the photoinduced damage of the anticancer drug gefitinib (GFT) and its two main photoactive metabolites GFT-M1 and GFT-M2 in cellular milieu was recently investigated. With this background, the photophysical properties of both the drug and its metabolites have now been studied in the presence of the two main transport proteins of human plasma, i.e., serum albumin (HSA) and α1-acid glycoprotein (HAG) upon UVA light excitation. In general, the observed photobehavior was strongly affected by the confined environment provided by the protein. Thus, GFT-M1 (which exhibits the highest phototoxicity) showed the highest fluorescence yield arising from long-lived HSA-bound phenolate-like excited species. Conversely, locally excited (LE) states were formed within HAG, resulting in lower fluorescence yields. The reserve was true for GFT-M2, which despite being also a phenol, led mainly to formation of LE states within HSA, and phenolate-like species (with a minor contribution of LE) inside HAG. Finally, the parent drug GFT, which is known to form LE states within HSA, exhibited a parallel behavior in the two proteins. In addition, determination of the association constants by both absorption and emission spectroscopy revealed that the two metabolites bind stronger to HSA than the parent drug, whereas smaller differences were observed for HAG. This was further confirmed by studying the competing interactions between GFT or its metabolites with the two proteins using fluorescence measurements. These above experimental findings were satisfactorily correlated with the results obtained by means of molecular dynamics (MD) simulations, which revealed the high affinity binding sites, the strength of interactions and the involved amino acid residues. In general, the differences observed in the photobehavior of the drug and its two photoactive metabolites in protein media are consistent with their relative photosensitizing potentials.

Comparing skin swabs, buccal swabs, and toe clips for amphibian genetic sampling, a case study with a small anuran (acris blanchardi)

Abstract Multiple methods for collecting genetic samples from amphibians exist, each with their own implications for study design, animal welfare, and costs. Toe clipping is one common method, but there is ongoing debate regarding its potential detriment. Less invasive methods should be implemented, if efficacious, as amphibians are a particularly vulnerable vertebrate group. Skin and buccal swabbing are less invasive methods for genetic sampling, but the potential for contamination and a lower yield of DNA may exist. To compare these methods, we gathered skin swabs, buccal swabs, and toe clips from the same individuals of a relatively small anuran species, Blanchard’s Cricket Frog (Acris blanchardi). We then compared DNA yield, DNA purity, amplification success rate, and genotypic data quality among sample types. We found toe clips and buccal swabs generated similar DNA yield and purity, with skin swabs yielding significantly less DNA of significantly lower purity than the other sample types. Amplification success rate was significantly higher using toe clips compared to the other sample types, though buccal swab samples amplified more readily than skin swabs. Genotypic data from toe clips and buccal swabs did not differ significantly in quality, but skin swab data quality was significantly lowest among sample types. Thus, skin swabbing could produce erroneous data in some situations, but buccal swabbing is likely an effective substitute to toe clipping, even for small species. Our results can help future researchers select which genetic sampling method might best suit their research needs.

Studies of Protein Binding to Biomimetic Membranes Using a Group of Uniform Materials Based on Organic Salts Derived From 8-Anilino-1-naphthalenesulfonic Acid.

Tuning the 8-anilino-1-naphthalenesulfonic acid (ANS) structure usually requires harsh conditions and long reaction times, which can result in low yields. Herein, ANS was modified to form an ANS group of uniform materials based on organic salts (GUMBOS), prepared with simple metathesis reactions and distinct cations, namely tetrabutylammonium (N4444), tetrahexylammonium (N6666), and tetrabutylphosphonium (P4444). These ANS-based GUMBOS were investigated as fluorescent probes for membrane binding studies with four proteins having distinct physicochemical properties. Liposomes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine were employed as membrane models as a result of their ability to mimic the structure and chemical composition of cell membranes. Changes in fluorescence intensity were used to monitor protein binding to liposomes, and adsorption data were fitted to a Freundlich-like isotherm. It was determined that [N4444][ANS] and [P4444][ANS] GUMBOS have enhanced optical properties and lipophilicity as compared to parent ANS. As a result, these two GUMBOS were selected for subsequent protein-membrane binding studies. Both [N4444][ANS] and [P4444][ANS] GUMBOS and parent ANS independently reached membrane saturation within the same concentration range. Furthermore, distinct fluorescence responses were observed upon the addition of proteins to each probe, which demonstrates the impact of properties such as lipophilicity on the binding process. The relative maintenance of binding cooperativity and maximum fluorescence intensity suggests that proteins compete with ANS-based probes for the same membrane binding sites. Finally, this GUMBOS-based approach is simple, rapid, and involves relatively small amounts of reagents, making it attractive for high-throughput purposes. These results presented herein can also provide relevant information for designing GUMBOS with ameliorated properties.

Is More Organic Farming a Responsible Strategy? An Appeal for Responsible (Sustainably Intensive) Agriculture

In politics and society, organic farming is widely seen as the ideal way to address the many challenges facing agriculture and food security. In order to examine the extent to which this view is realistic, the impact of organic and conventional farming is compared on the basis of relevant criteria such as land requirements, greenhouse gas emissions, biodiversity, food quality, production and environmental costs, reduced meat consumption, the need for agricultural imports and the global expansion of arable land. Land is considered to be a central factor. Such a comprehensive analysis is necessary to assess the impact of different influencing factors and to provide important suggestions for targeted decision making. Although organic farming has a positive impact on land use in many ways, it quickly reaches its limits. The low yields and high production costs resulting from the limited choice of means of production make organic farming an unsuitable model for the future in a world with limited arable land, a high and growing demand for food, and low incomes for large sections of the population. It can, however, provide significant impetus and contribute important technological knowledge for the further development of agriculture as a whole to meet the complex objectives of sustainability, greenhouse gas mitigation, biodiversity and global food security by incorporating all available technologies. Based on this, a model for responsible, sustainable intensive agriculture is outlined and proposals for an eco-social framework to support this model are put forward.

Antibacterial activity of aqueous and methanol extracts of &lt;i&gt;Jatropha tanjorensis&lt;/i&gt; and &lt;i&gt;Adansonia digitata&lt;/i&gt; against selected clinical bacteria pathogens

The powdered plant parts (bark, stem, and leaf) were extracted with methanol, and cold and hot water using the maceration method. A modified agar well diffusion technique was used for the assessment of the antibacterial activities of the aqueous and methanol extracts of J. tanjorensis and A. digitata. The methanol extract of A. digitata bark gave the highest yield of 75 % while the lowest yield was observed with the cold water extract of A. digitata bark of 33 %. The phytochemical screening showed an abundance of alkaloids, terpernoids, saponins, flavonoids, tannin, glycoside, and phenol. The bark, leaf, and stem of J. tanjorensis and A. digitata showed varying degrees of antibacterial activities. against the Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteus spp, and Klebsiella spp) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes and Streptococcus pneumonia). The bark, stem, and leaf extract of J. tanjorenis significantly (p &lt; 0.05) inhibited all the pathogens except P. aeruginosa and S. aureus.The result of the minimum bactericidal concentration MBC for the combined extracts of A. digitata and J. tanjorenis subfractions showed that the extracts have greater antibacterial activities at concentrations not lesser than 100 mg/ml. Thus, J. tanjorensis and A. digitata could be used as potent herbal remedies to mitigate the adverse effects of Gram-negative and positive clinical pathogens. Thus, the aim of this study was to evaluate the antibacterial activity of aqueous and methanol extracts of Jatropha tanjorensis and Adansonia digitata against selected Clinical bacteria pathogens.

Artisanal and Small-Scale Gold Mining Impact on Soil and Agriculture: Evidence from Upper Denkyira East Municipality, Ghana

The gold mining industry in Ghana has made a substantial contribution to the country's economic growth by generating diverse employment opportunities, both directly and indirectly, in mining communities. This has the potential to mitigate poverty and improve the quality of life for individuals and households in rural areas, where climate change is having a disproportionate impact on small-scale farmers. Nevertheless, it is crucial to recognize that this sector has also resulted in detrimental environmental impacts. A total 150 key informants in Upper Denkyira East Municipality, comprising representatives from the Environmental Protection Agency, the Forestry Commission, the Mineral Commission, and other stakeholders, were interviewed using standardize questionnaire to ascertain the impact of mining on the soil, agriculture and the environment. The study also employed inductively coupled plasma to assess the concentration of heavy metals in soils at a small-scale mining site. The soil analyses indicated high concentrations of heavy metals, such as mercury, arsenic, nickel, zinc, and iron. The Weighted Average Index, which was used to assess the impact of mining, revealed that communities face challenges such as deforestation, low crop yields, water pollution, land tenure issues, and a high cost of living. The study's analyses of measures to address small scale mining challenges revealed that alternative livelihood, law enforcement, training and support, and access to mining concessions were perceived as suitable for addressing artisanal and small scale mining. The study concluded that stricter mining regulations, promoting sustainable mining practices, and providing comprehensive training to miners on environmental protection and land reclamation can help mitigate the negative impacts of artisanal and small-scale mining. Additionally, supporting alternative means of livelihood for mining communities, such as agriculture or eco-tourism, can reduce reliance on mining and promote sustainable development.

Hysteretic behavior of the segmented buckling‐resistant braces with LYP160

AbstractThe goal was to evaluate the hysteretic performance of buckling‐resistant braces with low yield point steel LYP160, the monotonic tensile and cyclic loading tests of LYP160 test specimens were conducted and the cyclic constitutive relationship was obtained. According to the load–displacement curves of the specimens, the low‐yield point steel was characterized by good ductility and energy absorption ability. With consideration of the Chaboche model for the materials, the cyclic hardening parameters of low‐yield point steel were obtained. On this basis, the hysteretic properties of buckling‐resistant braces under cyclic loads were simulated and analyzed. After the analysis and comparison of buckling‐resistant braces specimens with isotropic core plate and segmented variable section core plate, it can be found that: when the conventional buckling‐resistant braces with an isotropic core plate were loaded to L/100, the lateral deformation of the buckling‐resistant brace (BRB) would reach 17 mm. Additionally, serious squeezing could be observed on the lateral restraining members. The conventional BRB would become ineffective due to the accumulation of deformation at both ends of the BRB. When the segmented buckling‐resistant brace was applied, the core plate with variable section would buckle first in the middle area, other parts could continue to consume energy thanks to the action of the limit plate. It would avoid the situation that other areas would be unable to consume energy after the core plate yields at one area first. Under the action of cyclic loads, no stiffness degradation was noted in the segmented buckling‐resistant brace. Segmented buckling‐resistant braces demonstrated superior ductility and energy dissipation capacity.

Catalysts for C-N coupling in urea electrosynthesis under ambient conditions from carbon dioxide and nitrogenous species.

Urea is an indispensable nitrogen-containing organic compound in modern human life. However, the current industrial synthesis of urea involves ammonia, which is produced through the Haber-Bosch process under harsh reaction conditions, causing huge energy consumption and heavy environmental pollution. Electrochemical reduction of carbon dioxide (CO2) and nitrogenous species (N2, NOx- and NO) have achieved significant progress, offering a promising approach for the electrochemical C-N coupling to produce urea under ambient conditions. Urea synthesis driven by renewable electricity represents a suitable alternative to the traditional process, contributing to the goal of carbon neutrality and nitrogen cycles. However, challenges such as low yield rate, poor selectivity and unveiled reaction mechanisms still need to be addressed. This review provides a summary of the latest catalysts utilized in urea electrosynthesis, aiming to provide guidance and prospects for the development of high-performance catalysts.