A reverse substrate bias operational amplifier using existing driver technology has been designed and implemented using 0.25μm CMOS process to achieve high noise performance. Op amps are efficient and versatile devices. Its applications include signal conditioning, custom conversion, analog equipment, competitive simulation, and various electronic industries meeting custom design requirements. As the trend of low voltage devices continues, designing high-performance analog circuits becomes increasingly difficult. The main module of analog circuits is the work amplifier. In large electronic devices, there is a trade-off between speed, power, and gain, among other drawbacks. Additionally, in order to reduce costs and integrate analog and digital circuits on a single die, analog designers must face the challenges of using the CMOS process. Design and implementation are done in 0.25μm technology using TSMC libraries and with the help of Tanner tools.