Phalaenopsis plants require several weeks of prolonged low-temperature periods for inflorescence initiation. We conducted a series of experiments to determine the inhibitory effects of intermittent high-temperature treatment on inflorescence initiation and the involvement of carbohydrate metabolism in the flowering inhibition of Phalaenopsis. In Expt. 1, Forcing temperatures from zero to ten weeks with two weeks of interval with different cultivars (‘Mantefon’, ‘Hong Seol’ and ‘Cutie’) or temperatures (constant 20 and 23 °C). After each treatment period, the plants were moved to 28 °C high temperature to prevent further low-temperature accumulation. In Expt. 2 and 3, under vegetative (continuous high temperature at 28/28 °C), forcing (continuous low temperature at 25/20 °C), and intermittent high-temperature conditions, growth and inflorescence initiation characteristics were observed, and transcriptomes and carbohydrate contents in leaves were also analyzed. Six to eight weeks of low-temperature periods were required for inflorescence initiation irrespective of cultivars, and different temperatures did not induce significant difference. The disruption of low-temperature exposure decreased the percentage of flowering-induced plants and delayed inflorescence emergence in all cultivars used in this study despite a sufficiently low-temperature period. Flowering-inhibited plants showed a significant increase in new leaf numbers, implying that vegetative growth was maintained by intermittent treatment. In transcriptome analysis, relatively low expression of differentially expressed genes (DEGs) of enzymes related to carbohydrate hydrolysis and gluconeogenesis was observed in vegetative and intermittent conditions. Moreover, the soluble sugar contents in leaves were significantly reduced under the intermittent condition compared with the forcing condition. These results indicated that discontinuous low-temperature exposure by intermittent high-temperature treatment prevented inflorescence initiation of Phalaenopsis and that the flowering inhibition could be attributed to the reduction of carbohydrate contents in leaves by decreasing sugar accumulation.