Identification of fly larvae species may offer valuable information as to the location, or the environment in which corpses were placed, but only if the geographical distribution of larva species is clarified. In this study, we investigated a total of 126 larvae on 42 corpses found in Saitama Prefecture in Japan between July and September. We identified the larva species by analyzing the sequences of mitochondrial DNA cytochrome oxidase gene subunit I. Our results revealed that larvae belonged to 6 different species: Lucilia sericata and Chrysomya pinguis from the Calliphoridae family, and Parasarcophaga crassipalpis, Boettcherisca peregrina, Parasarcophaga harpax, and Parasarcophaga dux from the Sarcophagidae family. Additionally, we investigated if there was a correlation between larvae species and population density. Based on the random sampling and the statistical analysis on the entire larva collection, larvae of Chrysomya pinguis species were more likely to be found in low population density areas, whereas larvae of Lucilia sericata were commonly found in high population density areas. The accumulation of distribution data of larvae may be useful to confirm the environment around the place where corpses were found.
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