Lower Peak Research Articles

Scleroderma renal crisis, an increasingly rare but persistently challenging condition: a retrospective cohort study

Abstract Objective Scleroderma renal crisis (SRC) is associated with high morbidity and mortality and there remain unmet needs regarding early identification and treatment. We aimed to assess risk factors for and the outcomes of SRC at a large Australian tertiary hospital. Methods Seventeen incident SRC cases were diagnosed between 2012–2022. Demographic, systemic sclerosis (SSc) manifestations, and treatment data were extracted. Using data from the Australian Scleroderma Cohort Study (ASCS) (n = 483), logistic regression analysis was performed to identify risk factors for SRC. Results The prevalence of SRC was 3.52%. The median SSc disease duration at SRC onset was 2 (IQR 1–4) years. Peak creatinine occurred at a median of 11 (IQR 5–14) days post-SRC diagnosis with a median peak creatinine of 144 (IQR 86–306) µmol/l. Nine (52.94%) SRC patients had evidence of acute neurologic and/or cardiac complications. Acute hemofiltration was required in 3 (17.65%) patients. Over the follow-up period, 7 (41.18%) SRC patients died 2.75 (IQR 0.74–7.25) years after SRC onset. Patients with SRC were more likely to be male (OR 9.73, 95%CI 3.57–26.56), have diffuse disease (OR 23.16, 95%CI 5.22–102.80), and have antibodies to Scl70 (OR 3.34, 95%CI 1.24–9.04) or RNA polymerase III (RNAPIII) (OR 5.15, 95%CI 1.91–13.89). Conclusion SRC is an uncommon manifestation, but outcomes remain poor. A significant proportion of patients presenting with SRC in Australia are positive for Scl70 or RNAPIII antibody. Despite relatively low peak serum creatinine and rates of renal replacement therapy, SRC was still associated with significant mortality.

Characterizing the features of the low-amplitude peaks in delta Scuti stars with TESS

Abstract The presence of low-amplitude peaks over the noise in the power spectra of δ Scuti stars is frequently disregarded. These seemingly insignificant peaks, collectively referred to as grass, might contain valuable information about the origin of these stars and the reasons behind the occurrence or absence of a plateau. It is crucial to systematically parameterize the grass phenomenon throughout a comprehensive sample that covers the entire δ Scuti star parameter range. Thus, we conduct a quantitative study of long-duration, high-duty-cycle TESS light curves, leading to improved detection methods for plateaus and a deeper understanding of their nature. This approach minimizes the impact of unresolved peaks caused by mode variations over time. Additionally, we present appropriate analysis techniques to mitigate window effects and identify and eliminate spurious peaks. We demonstrate here that the grass can be effectively parameterized based on peak density. With such parameterization two distinct regimes are found: the sparse grass regime, characterized by low peak density and the absence of a plateau in the power spectra, and the dense grass regime, characterized by high peak densities and the presence of an observable plateau. Our study is the first rigorous quantification of the emergence of such a plateau in the power spectra of δ Scuti stars. Since the grass might be related with fractality, mode variability, and stellar rotation rate, its parameterization opens a new way to analyze these stars.

LPI radar waveform design based on complementary phase and discrete chaotic frequency joint coding

In order to reduce the probability of radar radiation signals being detected by enemy passive detection systems, this paper proposes a phase and frequency joint coding low intercept Radar waveform design method. Based on the linear frequency modulation signal, this method uses complementary binary code and chaotic sequence to phase encode the intra-pulse modulation. Code and frequency coding. The numerical simulation results show that the designed waveform exhibits pseudo-random characteristics in the time-frequency domain, and the low recognition performance is improved; The signal has an extremely low peak sidelobe level after matched filtering, showing excellent low intercept performance; its three-dimensional ambiguity function diagram presents an ideal “graph”. Fishing type”, with good distance, speed resolution and anti-interference characteristics.

Microsatellite density landscapes illustrate short tandem repeats aggregation in the complete reference human genome

BackgroundMicrosatellites are increasingly realized to have biological significance in human genome and health in past decades, the assembled complete reference sequence of human genome T2T-CHM13 brought great help for a comprehensive study of short tandem repeats in the human genome.ResultsMicrosatellites density landscapes of all 24 chromosomes were built here for the first complete reference sequence of human genome T2T-CHM13. These landscapes showed that short tandem repeats (STRs) are prone to aggregate characteristically to form a large number of STRs density peaks. We classified 8,823 High Microsatellites Density Peaks (HMDPs), 35,257 Middle Microsatellites Density Peaks (MMDPs) and 199, 649 Low Microsatellites Density Peaks (LMDPs) on the 24 chromosomes; and also classified the motif types of every microsatellites density peak. These STRs density aggregation peaks are mainly composing of a single motif, and AT is the most dominant motif, followed by AATGG and CCATT motifs. And 514 genomic regions were characterized by microsatellite density feature in the full T2T-CHM13 genome.ConclusionsThese landscape maps exhibited that microsatellites aggregate in many genomic positions to form a large number of microsatellite density peaks with composing of mainly single motif type in the complete reference genome, indicating that the local microsatellites density varies enormously along the every chromosome of T2T-CHM13.

Decoding the Early Universe: Exploring a Merger Scenario for the High-Redshift Cluster JKCS041 using Numerical Models

Abstract JKCS041 (z = 1.8) is one of the most distant galaxy cluster systems known, seen when the Universe was less than 4 billion years old. Recent Sunyaev-Zeldovich (SZ) observations show a temperature decrement that is less than expected based on mass estimates of the system from X-ray, weak gravitational lensing and galaxy richness measurements. In this paper we seek to explain the observables - in particular the low SZ decrement and single SZ peak, the projected offset between the X-ray and SZ peaks of ≈220 kpc, the gas mass measurements and the lensing mass estimate. We use the GAMER-2 hydrodynamic code to carry out idealized numerical simulations of cluster mergers and compare resulting synthetic maps with the observational data. Generically a merger process is necessary to reproduce the observed offset between the SZ and X-ray peaks. From our exploration of parameter space, seen a few tenths of a Gyr after first core passage, two components with total mass of ≈2 × 1014M⊙, mass ratio of ≈2:3, gas fraction of 0.05 − 0.1 and Navarro, Frenk and White (NFW) mass density profile concentrations c≈5 are scenarios that are consistent with the observational data. For consistency with the SZ and X-ray measurements, our simulations exclude total mass in excess of ≈3 × 1014M⊙, primarily based on the SZ signal. The mass ratio is constrained by the SZ-X-ray offset and magnitude of the SZ signal, ruling out systems with equal and vastly different masses.

Peak expiratory flow predicts motoric cognitive risk syndrome: A cohort study.

Poor respiratory function, including low peak expiratory flow (PEF), is a risk factor for dementia. Motoric cognitive risk syndrome (MCR) is a predementia syndrome characterized by slow gait and subjective cognitive complaints. However, the association between PEF and MCR remains unclear. This study examined the cross-sectional and longitudinal association between PEF and MCR. The National Health and Aging Trends Study (NHATS), which is a nationally representative cohort of adults ≥65 years of age in the United States, was analyzed from 2011 to 2017. Logistic regression and discrete-time proportional hazards models tested the association of PEF standardized residuals (SR) at baseline with prevalent and incident MCR, respectively. The models adjusted for multiple sociodemographic and health-related covariates. Among 5328 participants (57% women) included at baseline, lower PEF SR was associated with higher prevalence of MCR. Compared with the ≥80 PEF SR percentile group, the <30 and 30-50 percentile groups had significantly higher odds of prevalent MCR (OR 3.04 [95% CI 1.85, 5.01]; OR 2.06 [95% CI 1.19, 3.54], respectively). Over six years of follow-up, lower PEF SR was also associated with higher incidence of MCR. Compared with the ≥80 PEF SR percentile group, the <30 and 30-50 percentile groups had significantly higher risk of incident MCR (HR 1.81 [95% CI 1.24, 2.66]; HR 1.55 [95% CI 1.02, 2.34], respectively). Lower PEF was associated with higher prevalence and incidence of MCR. Poor respiratory function should be further investigated as a potentially modifiable risk factor for MCR and cognitive decline.

Peak alpha frequency differs between chronic back pain and chronic widespread pain.

Low peak alpha frequency (PAF) is an electroencephalography (EEG) outcome associated reliably with high acute pain sensitivity. However, existing research suggests that the relationship between PAF and chronic pain is more variable. This variability could be attributable to chronic pain groups typically being examined as homogenous populations, without consideration being given to potential diagnosis-specific differences. Indeed, while emerging work has compared individuals with chronic pain to healthy controls, no previous studies have examined differences in PAF between diagnoses or across chronic pain subtypes. To address this gap, we reanalysed a dataset of resting state EEG previously used to demonstrate a lack of difference in PAF between individuals with chronic pain and healthy controls. In this new analysis, we separated patients by diagnosis before comparing PAF across three subgroups: chronic widespread pain (n = 30), chronic back pain (n = 38), and healthy controls (n = 87). We replicate the original finding of no significant difference between chronic pain groups and controls, but also find that individuals with widespread pain had significantly higher global average PAF values than those of people with chronic back pain [p = 0.028, β = 0.25 Hz] after controlling for age, sex, and depression. These novel findings reveal PAF values in individuals with chronic pain may be diagnosis-specific and not uniformly shifted from the values of healthy controls. Future studies should account for diagnosis and be cautious with exploring homogenous 'chronic pain' classifications during investigations of PAF. Our work suggests that, contrary to previous hypotheses, inter-individual differences in PAF reflect diagnosis-specific mechanisms rather than the general presence of chronic pain, and therefore may have important implications for future work regarding individually-tailored pain management strategies.

Ring-assisted, racetrack, and Mach-Zehnder modulator: which one offers the lowest voltages and chirp-free operation?

This study presents a comparison between resonant and non-resonant electro-optical modulator configurations. The focus lies on finding the configuration with the highest modulation amplitude at the lowest drive voltage while achieving a large electro-optical bandwidth. It is found that the ring-assisted Mach-Zehnder modulator (RaMZM) offers chirp-free and resonantly enhanced modulation without bandwidth limitations imposed by the ring. In contrast, a racetrack modulator (RTM) offers resonant enhancement at the cost of a chirped modulated signal and with a bandwidth limitation. The traditional non-resonant Mach-Zehnder modulator (MZM) configuration requires higher modulation voltages while offering chirp-free operation with a flat frequency response. The RaMZM, therefore, looks like an ideal candidate for encoding information in backbone networks where small modulation voltages and perfect control over the phase of a signal are needed. The results are supported by experiments that show driverless plasmonic modulation at 220 GBaud 2PAM, 160 GBaud 4PAM and 100 GBaud 8PAM with record low peak voltages of 0.5 V.

8517 Low Peak GH in Stimulation Tests Among Obese Children; Is it Clinically Meaningful?

Abstract Disclosure: D. Strich: None. C. Meirman: None. S. Edri: None. D.J. Gillis: None. Background: Peak growth hormone (GH) levels in stimulation tests are lower in obese subjects. The clinical significance of this finding is unclear. It has been suggested that results of tests should be adjusted for body mass index (BMI). Aims: A. To evaluate the association between GH peak levels and body mass index (BMI ) in a single center. B. To discuss the biological and clinical significance of the findings and make recommendations based on this study and other data from the medical literature. Methods: Peak GH levels and BMI standard deviation scores (BMI-SDS) for a total of 988 tests (Clonidine - 694, Glucagon - 198, Arginine - 96) performed in one center were analyzed. Studies referring to the biology of growth hormone and to the evaluation of GH deficiency were reviewed. Results: In the testing center studied, mean peak GH levels were significantly lower in arginine (p=0.015) and clonidine tests (p=0.018) starting at BMI standard deviation score over 1 compared to below 1 BMI-SDS. The differences still remained significant for a subgroup with peaks below 7.5 ng/dl (p=0.003 and p=0.042 respectively). The proportion of overweight and obese children in our cohort and in similar cohorts of children undergoing stimulation tests was about tenfold lower than in the corresponding general population. In other studies, short obese patients responded better than non-obese to GH treatment and had greater increases both in height and IGF-1. Obese adult patients with low GH peaks had high carotid intima-media thickness compared with non GH deficient obese. Conclusion: Obese children are generally tall and therefore are not referred for testing. Those who are short, tend to have lower peaks than their lean peers in GH stimulation tests and respond better to GH therapy. These patients have biologically significant GH deficiency. We do not recommend "correcting" GH test results for BMI in the decision algorithm for GH therapy. Presentation: 6/3/2024

7640 The first description of an MC4R variant in a patient with Kallmann syndrome and obesity

Abstract Disclosure: A.A. Aslam: None. S. Lim: None. R. Willemsen: None. K. Koysombat: None. M. Young: None. W.S. Dhillo: None. A. Abbara: None. S. Howard: None. E.F. Gevers: None. Introduction: Pathogenic MC4R gene variants result in hyperphagia and early onset obesity but puberty is not usually affected. We describe an MC4R variant in a patient with Kallmann syndrome and obesity. A 16 year old male with repaired Tetralogy of Fallot, anosmia, autism and anxiety, was referred with obesity and delayed puberty. His birth weight was 3360 g (-0.26 SDS). Height was -1.31 SDS, BMI 30.7 kg/m2. He had high arched palate, normal skin and hair colour, mild hypertelorism and upward slanting palpebral fissures. Pubertal staging was A2P1G1 5ml testes bilaterally. Results: LHRH test showed borderline low peaks (LH 5.0 U/L, FSH 2.6 U/L) and inhibin B 108 pg/mL (25-325 pg/mL). MRI brain showed hypoplastic olfactory bulbs, absent olfactory sulci and normal pituitary anatomy. CGH microarray, karyotype and the UK hypogonadism gene panel were normal. He underwent whole genome sequencing in the Genetic Factors Affecting the Timing of Puberty Study; no abnormalities in 52 known genes associated with GnRH deficiency were found but a previously described heterozygous variant was detected, MC4R c.542G&amp;gt;A, p.Gly181Asp, classified pathogenic and absent in control databases. Management: He was commenced on Testosterone but showed progression of testicular size to 10 mL and testosterone was stopped but required restarting. Repeat investigations off treatment, aged 22 years (height 178 cm, BMI 42 kg/m2, testes 15 mL): inhibin B 66 pg/mL, testosterone 2 nmol/L, baseline LH 2.4 U/L, stimulated peak 24.9 U/L, baseline FSH 1.2 U/L, stimulated peak 4.2 U/L. Cortisol and IGF1 were in the normal range. He also developed autoimmune hypothyroidism with raised TPO antibodies (TSH 24.7 mU/L, FT4 5.6 pmol/L). Discussion: Previous in vitro work has shown complete loss of function of MC4R p.Gly181Asp due to reduced cell surface expression and reduced binding to a-MSH. Heterozygous p.Gly181Asp variants have been described in several children/adults with obesity; but hypogonadism in heterozygous carriers has not. Homozygous MC4R p.Gly181Asp was found in a male with obesity and partial HH thought to be due to abnormal GnRH production but with normal olfactory bulbs. We are the first to describe a heterozygous MC4R p.Gly181Asp variant in a patient with partial hypogonadism and anosmia with hypoplastic bulbs in addition to obesity. Interaction between POMC-MC-leptin circuits and Kisspeptin-GnRH circuits is recognised although not completely understood. Previous work has shown that a subset of kisspeptin neurons express MC4R and that KNDy neurones receive synaptic input from POMC neurons. In addition, inhibition of MC4R/MC3R results in delayed onset of puberty in rats. Our results support a role for MC4R in GnRH secretion and potentially olfactory /GnRH neuronal migration. Assessment of spontaneous gonadotrophin production and response of gonadotrophin production to kisspeptin stimulation is currently in progress. Presentation: 6/1/2024

Fabrication of transparent silica glass and evaluation of silica particle dispersibility in silica slurries based on the solubility parameters of the slurry components

Silica particles were dispersed in 11 acrylic monomers at various concentrations. The dispersibility of the silica particles was evaluated based on the viscosity of the slurry. Furthermore, the relationship between the viscosity of the slurry and the solubility parameters (SPs) of the silica particles and acrylic monomers was investigated. When the SPs of the silica particles and acrylic monomers were close, the viscosity of the silica slurry decreased and high concentrations of the silica particles could be dispersed in the acrylic monomers. Therefore, SP can be used for predicting the dispersibility of silica particles. The prepared slurry was then used to fabricate sintered silica glass. Silica slurry was cured through photopolymerization to obtain a green body, which was then heat-treated at 850°C in air atmosphere and sintered at 1710°C under vacuum to obtain sintered silica glass. Transparent sintered silica glass with no cracks was obtained using 4-hydroxybutyl acrylate, which has a similar SP to that of silica particles, as a solvent. The obtained sintered silica glass exhibited a transmittance of 92% at a wavelength of 400 nm. In the ultraviolet region, light absorption originated from two-coordinated Si in the glass. The absorption spectrum in the infrared region showed an extremely low peak of the SiOH group, indicating that low-OH silica glass was obtained. Thus, this study provides a new method for fabricating silica glass and provides a method of selecting solvents for the slurries used in powder sintering.

Similarity of the low-occurrence wind profiles within urban turbulent boundary layers of uniform and non-uniform height block arrays

Within urban boundary layers, the safety of pedestrians is markedly affected by wind speed, particularly in urban areas. The characteristics of turbulence above the canopy layers can lead to unpredictable changes in wind speed at the pedestrian level. Therefore, this study analyzes low-occurrence wind speed phenomena above canopy heights for uniform and nonuniform block configurations using wind tunnel experiments (WTE) to understand the background turbulence characteristics which the wind profile is generated by the boundary layer above the canopy. The urban canopy arrays were reproduced using solid blocks arranged in 30 rows in a streamwise direction with a packing density of 25 % at three different heights. An x-type hot-wire anemometer was used to measure the streamwise and vertical velocity components. The low-occurrence values were classified based on wind speed percentiles of 0.1 %, 1.0 %, 99.0 %, and 99.9 % wind speeds. The results demonstrated that above the canopy, there were minor influences of block height variations on the low occurrence factor. The peak factor demonstrated a comparable value between the uniform and nonuniform cases, regardless of the block arrangement. Statistical models based on the Weibull distribution and Gram–Charlier series demonstrating good agreement with the WTE data in predicting the low occurrence and peak factors. This study found that variations in block height have a minor influence on the low occurrence and peak factors within the turbulent boundary layers, implying that we can separate the effect of background turbulence from the local turbulent generation within the urban canopy layers.

Causes on the 2018 record-breaking heatwave in South Korea: Compound impacts from recurrent large-scale atmospheric teleconnections

Abstract This study analyzed the causes of the extreme heatwave in East Asia in 2018, which brought a record-breaking event in South Korea in terms of the number of hot days and the intensity in history. Long-lasting atmospheric patterns were observed during the 2018 heatwave, and the patterns were similar to those known as the Pacific-Japan (PJ), Circum-global Teleconnection (CGT), and Arctic Oscillation (AO) patterns identified in a previous study. In 2018, all three patterns appeared to exhibit a strong positive phase. In particular, the PJ and AO Indices showed the highest values within the analysis period, having a major impact on the heatwave. According to a quantitative analysis through multi-linear regression (MLR) of how much each of the three patterns affects the heatwave, all three patterns of PJ, CGT, and AO have significant effects on the heatwave in Korea. The statistically reconstructed heatwave days (HWD) time series with the three indices by MLR account for 51% of the total variability at the interannual timescale. Especially in 2018, most of the occurred HWD could be restored using the three indices. Also, in 1994, when the three indices had a high peak, the number of HWDs was the second-longest ever, while in 1993, when all three indices had a low peak, the heatwave was the lowest. This study quantitatively examined whether the large-scale extreme heatwave in 2018 greatly increased because of PJ, CGT, and AO. This study suggests that extreme heatwave can occur again if three apparently independent atmospheric patterns appear at once in the future.

Synergistic impact of scan strategy and scan angles in laser powder bed fusion process on mechanical, tribological and cell viability properties of porous 316L SS structure

Poor load bearing ability and concentrated stress within cross-linked porous structures was found to be the major cause for the mechanical and wear failure of SS (Stainless Steel) 316L implants. Therefore, the present study introduces a novel scan pattern to enhance the mechanical and wear properties. Importantly, the role of porous structures on the constituents and formation of Tribo-film regimes under the simulated body fluid (SBF) condition were assessed using the X-ray photoelectron spectroscopy (XPS). Furthermore, the impact of the surface roughness of porous structures on the cell adhesion and cytotoxicity were explored. Various cross-linked porous structures are produced at three different scanning rotation angles (0°, 45°, and 90°) using the Laser powder bed fusion (LPBF) process. Morphology analysis reveals balling and stair-case effects at 0°, controlled for structures built at 45° and 90°. Porous-90° structure exhibits negative skewness and a platykurtic surface with low peaks and more valleys. It demonstrates higher strength with low density due to better particle fusion, net-like structure, and uniform element distribution. X-ray photoelectron spectroscopy (XPS) evaluates the impact of porous structures on Tribo-film regimes in simulated body fluid. Surface roughness effects on cell adhesion and cytotoxicity are also investigated. Cross linked porous structures presented two major compression failure modes include wrinkling and diagonal shearing. The densified structure of Porous-45° reduces contamination and cell death, facilitating stable cell growth. Tribo-film formation and load bearing ability of Porous-90° structure was better than the other structures, due to the cross-linked netlike structure and further more detailed in the XPS study.

Effects of post-curing light intensity on the trueness, compressive strength, and resin polymerization characteristics of 3D-printed 3-unit fixed dental prostheses.

To investigate the effect of different post-curing light intensities on the trueness, compressive strength, and resin polymerization of 3D-printed 3-unit fixed dental prostheses (FPD). A total of 60 specimens were prepared to support a 3-unit FDP with a deep chamfer marginal design, utilizing computer-aided design and computer-aided manufacturing (CAD-CAM) technology. Light-polymerizing FDP resin with varying light intensities (105, 210, 420, and 840mW/cm2) was employed for 10 min. Subsequently, trueness assessment, fracture load testing, scanning electron microscopy (SEM) surface examination, and Fourier-Transform Infrared (FTIR) analysis were conducted. A one-way analysis of variance (ANOVA) was performed to ascertain the differences between the experimental groups (p < 0.05). The group exposed to 210mW/cm2 showed the highest trueness (57.6 ± 2.1 µm), while the 840mW/cm2 group had the highest deviation (79.3 ± 2.7 µm) (p < 0.001). Significant differences in fracture resistance were found between groups (p < 0.001), with mean fracture strengths of 1149.77 ± 67.81 N, 1264.92 ± 39.06 N, 1331.34 ± 53.62 N, and 1439.93 ± 34.58 N for light intensities of 105, 210, 420, and 840mW/cm2, respectively (p < 0.001). The resin polymerization analysis shows a peak intensity surge at 3579 cm-1 for O-H and C-H stretching vibrations, except in samples exposed to 105 mw/cm2 light, with the lowest peak at 2890 cm-1. The performance of resin polymerization is most significant under the condition of 840mW/cm2. The light intensity of 210mW/cm2 exhibited the highest trueness, while the 840mW/cm2 group showed the highest deviation. However, the light intensity of 840mW/cm2 demonstrated the highest compressive strength. Furthermore, polymerization occurred at all post-treatment light intensities except 105mW/cm2. These findings indicate that while low-intensity usage offers greater trueness, high-intensity usage provides better compressive strength and polymerization. Therefore, 210mW/cm2 could be the recommended solution for post-curing.

Reference gene evaluation for normalization of gene expression studies with lymph tissue and node‑derived stromal cells of patients with oral squamous cell carcinoma.

Profiling studies using reverse transcription quantitative PCR (RT-qPCR) require reliable normalization to reference genes to accurately interpret the results. A stable reference gene panel was established to profile metastatic and non-metastatic lymph nodes in patients with oral squamous cell carcinoma. The stability of 18S ribosomal RNA (18SrRNA), ribosomal Protein Lateral Stalk Subunit P0 (RPLP0), ribosomal Protein L27 (RPL27), TATA-box binding protein (TBP), hypoxanthine phosphoribosyl-transferase 1 (HPRT1), beta-actin (ACTB), glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and vimentin (VIM) was evaluated, as reference genes for profiling patient-derived lymph node stromal cells (LNSCs; N=8; N0:6, N+:2) and lymph node tissues (Patients:14, Nodes=20; N0:7; N+:13). The genes were initially assessed based on their expression levels, specificity, and stability rankings to identify the best combination of reference genes. VIM was excluded from the final analysis because of its low expression (high quantification cycle >32) and multiple peaks in the melting curve. The stability analysis was performed using Reffinder, which utilizes four tools; geNorm, NormFinder, BestKeeper and Comparative ∆Ct methods, thereby enabling the computing of a comprehensive ranking. Evaluation of the gene profiles indicated that while RPLP0 and 18SrRNA were stable in both lymph node tissues and LNSCs, HPRT1, RPL27 were uniquely stable in these tissues whereas ACTB and TBP were most stable in LNSCs. The present study identified the most stable reference gene panel for the RT-qPCR profiling of lymph node tissues and patient-derived LNSCs. The observation that the gene panel differed between the two model systems further emphasized the need to evaluate the reference gene subset based on the disease and cellular context.

Feasibility of the Compton Suppressed Gamma-ray spectrometry for proton therapy applications

The presence of the Bragg peak and its use in proton therapy cause less damage to healthy tissues. For optimal use of the proton beam in proton therapy, it is necessary to monitor the deviation of its range during treatment. The prompt gamma rays originated from the nuclear reactions of the proton beam with tissue elements can be applied for this purpose. The spatial distribution of prompt gamma rays can be used to estimate the range of the incident charged particles. Detecting a wide energy range of gamma rays, up to 10 MeV, is the most challenging step of the proton dose verification by using prompt gamma spectroscopy. Compton suppression is a system that is used to reduce the contribution of scattered gamma rays. This technique will cause better detection of the peaks of low-energy prompt gamma rays. In this research, the performance of a Compton suppression system has been investigated in two fields for proton therapy and for elemental analysis of materials using isotopes by a wide range of gamma energies. At first, by using the simulated Compton suppression system, the calculations of the prompt gamma ray spectra emitted from the brain phantom during the radiation of 150 MeV protons were presented. The results indicated that the low energy elements peaks such as 40Ca (1.37 MeV) with CSF equal to 1.138 appeared in the suppressed spectrum. Also, it was possible to detect 14N (2.31 MeV) and 16O (6.13 MeV) peaks equivalent to the CSF 2.449 and 2.067 respectively. In the next step, it was possible to detect the low energy peaks of 152Eu and 155Eu isotopes in the suppressed spectrum. For this purpose, the minimum relative intensity required to detect all 152Eu and 155Eu peaks was equivalent to 0.001 and 0.002 respectively. CSF for the lowest energies such as 105 keV and 344 keV was equal to 1.169 and 1.184. The simulation was done using the GEANT4 Monte-Carlo toolkit.

Crashworthiness design for novel polygonal asymmetric origami tubes

Origami tubes have attracted widespread attention because it is inclined to deform in the complete diamond mode (DM) with a low initial peak force Fmax and high average crushing force Fave. Although conventional origami tubes can improve crashworthiness, there are still many areas that fail to generate traveling plastic hinge lines, and the crashworthiness can be further improved. This paper presents a novel polygonal asymmetric origami tube (PAT) with origami patterns that can trigger more traveling plastic hinge lines compared to those of conventional origami patterns. The theoretical analysis of the deformation mechanism suggests that the PAT can realize hierarchical deformation and avoid stress concentration at sharp corners. The quasi-static experiments and numerical simulations reveal that the novel origami pattern can successfully trigger up to four times more traveling plastic hinge lines than the conventional square box (CSB) under the premise that each plastic hinge line is similar in length. Compared with the CSB, the PAT can deform in DM with a 56.0 % reduction of Fmax and a 59.1 % increase of Fave. Notably, the stableness of load-carrying capacity SLC is 90.2 %, which is 260.8 % higher than the CSB. A series of parametric studies reveal that the dihedral angle ratio α / β and the width-to-thickness ratio c / t of the PAT have a significant effect on crashworthiness. A smaller ratio of α / β and c / t provides the PAT with superior crashworthiness. Comparing the PAT with other types of origami tubes and multi-cell tubes indicates that the PAT also exhibits outstanding crashworthiness. The impact experiments also unveil a significantly enhanced crashworthiness of the PAT. Compared with the CSB, the Fmax decreases by approximately 46.4 %, while the Fave increases by around 92.7 %. The SLC and SEA values for the PAT surpass those of the CSB by about 260.8 % and 93.1 %, respectively. Furthermore, the theoretical analysis for predicting the Fave of the PAT is developed, with a maximum error not exceeding 8.5 %.

Taxonomic revision of Cantharellus cuticulatus Corner (Cantharellaceae, Basidiomycota) from Sabah (North Borneo), Malaysia

Cantharellus cuticulatus, first described by E.J.H. Corner in 1966, is an edible mushroom distinguished by its vibrant yellow-orange cap, lamellae-like hymenium and the absence of clamp connections. This study reveals a significant shift in its distribution from the high-altitude regions of Mount Kinabalu to lowland areas, raising interesting questions about its habitat preferences and associated host plants. The research was conducted between 2021 and 2023, involving comprehensive macro- and micro-morphological analyses, as well as molecular identification techniques. After a hiatus of over half a century without any new records in Sabah, the study has successfully collected three Cantharellus cuticulatus specimens from three different districts in Sabah. To date, this species has only been documented in Peninsular Malaysia and Northern Borneo (Sabah, Sarawak and Brunei).

Elevated Leukocyte Count and Platelet-Derived Thrombogenicity Measured Using the Total Thrombus-Formation Analysis System in Patients with ST-Segment Elevation Myocardial Infarction.

High platelet-derived thrombogenicity during the acute phase of ST-segment elevation myocardial infarction (STEMI) is associated with poor outcomes; however, the associated factors remain unclear. This study aimed to examine whether acute inflammatory response after STEMI affects platelet-derived thrombogenicity. This retrospective observational single-center study included 150 patients with STEMI who were assessed for platelet-derived thrombogenicity during the acute phase. Platelet-derived thrombogenicity was assessed using the area under the flow-pressure curve for platelet chip (PL-AUC), which was measured using the total thrombus-formation analysis system (T-TAS). The peak leukocyte count was evaluated as an acute inflammatory response after STEMI. The patients were divided into two groups: the highest quartile of the peak leukocyte count and the other three quartiles combined. Patients with a high peak leukocyte count (＞15,222/mm3; n=37) had a higher PL-AUC upon admission (420 [386-457] vs. 385 [292-428], p=0.0018), higher PL-AUC during primary percutaneous coronary intervention (PPCI) (155 [76-229] vs. 96 [29-170], p=0.0065), a higher peak creatine kinase level (4200±2486 vs. 2373±1997, p＜0.0001), and higher PL-AUC 2 weeks after STEMI (119 [61-197] vs. 88 [46-122], p=0.048) than those with a low peak leukocyte count (≤ 15,222/mm3; n=113). The peak leukocyte count after STEMI positively correlated with PL-AUC during primary PPCI (r=0.37, p＜0.0001). A multivariable regression analysis showed the peak leukocyte count to be an independent factor for PL-AUC during PPCI (β=0.26, p=0.0065). An elevated leukocyte count is associated with high T-TAS-based platelet-derived thrombogenicity during the acute phase of STEMI.