Low Oxygen Environment Research Articles

A Histone Methylation-MAPK Signaling Axis Drives Durable Epithelial-Mesenchymal Transition in Hypoxic Pancreatic Cancer.

Integrated regulation of histone methylation and MAPK signaling by the low-oxygen environment of pancreatic cancer drives long-lasting EMT that promotes chemoresistance and shortens patient survival and that can be pharmacologically inhibited. See related commentary by Wirth and Schneider, p. 1739.

Ginsenoside Rg1 alleviates vascular remodeling in hypoxia-induced pulmonary hypertension mice through the calpain-1/STAT3 signaling pathway

BackgroundHypoxic pulmonary hypertension (HPH) is the main pathological change in vascular remodeling, a complex cardiopulmonary disease caused by hypoxia. Some research results have shown that ginsenoside Rg1 (Rg1) can improve vascular remodeling, but the effect and mechanism of Rg1 on hypoxia-induced pulmonary hypertension are not clear. The purpose of this study was to discuss the potential mechanism of action of Rg1 on HPH. MethodsC57BL/6 mice, calpain-1 knockout mice and Pulmonary artery smooth muscle cells (PASMCs) were exposed to a low oxygen environment with or without different treatments. The effect of Rg1 and calpain-1 silencing on inflammation, fibrosis, proliferation and the protein expression levels of calpain-1, STAT3 and p-STAT3 were determined at the animal and cellular levels. ResultsAt the mouse and cellular levels, hypoxia promotes inflammation, fibrosis, and cell proliferation, and the expression of calpain-1 and p-STAT3 is also increased. Ginsenoside Rg1 administration and calpain-1 knockdown, MDL-28170, and HY-13818 treatment showed protective effects on hypoxia-induced inflammation, fibrosis, and cell proliferation, which may be associated with the downregulation of calpain-1 and p-STAT3 expression in mice and cells. In addition, overexpression of calpain 1 increased p-STAT3 expression, accelerating the onset of inflammation, fibrosis and cell proliferation in hypoxic PASMCs. ConclusionGinsenoside Rg1 may ameliorate hypoxia-induced pulmonary vascular remodeling by suppressing the calpain-1/STAT3 signaling pathway.

Development and Metabolomic Profiles of Bactrocera dorsalis (Diptera: Tephritidae) Larvae Exposed to Phytosanitary Irradiation Dose in Hypoxic Environment Using DI-SPME-GC/MS.

X-ray irradiation and modified atmospheres (MAs) provide eco-friendly, chemical-free methods for pest management. Although a low-oxygen atmospheric treatment improves the performance of some irradiated insects, its influence on the irradiation of quarantine insects and its impacts on pest control efficacy have yet to be investigated. Based on bioassay results, this study employed direct immersion solid-phase microextraction (DI-SPME) combined with gas chromatography-mass spectrometry (GC-MS) to determine metabolic profiles of late third-instar B. dorsalis larvae under normoxia (CON, Air), hypoxia (95% N2 + 5% O2, HY), super-hypoxia (99.5% N2 + 0.5% O2, Sup-HY), irradiation-alone (116 Gy, IR-alone), hypoxia + irradiation (HY + IR) and super-hypoxia + irradiation (Sup-HY + IR). Our findings reveal that, compared to the IR-alone group, the IR treatment under HY and Sup-HY (HY + IR and Sup-HY + IR) increases the larval pupation of B. dorsalis, and weakens the delaying effect of IR on the larval developmental stage. However, these 3 groups further hinder adult emergence under the phytosanitary IR dose of 116 Gy. Moreover, all IR-treated groups, including IR-alone, HY + IR, and Sup-HY + IR, lead to insect death as a coarctate larvae or pupae. Pathway analysis identified changed metabolic pathways across treatment groups. Specifically, changes in lipid metabolism-related pathways were observed: 3 in HY vs. CON, 2 in Sup-HY vs. CON, and 5 each in IR-alone vs. CON, HY + IR vs. CON, and Sup-HY + IR vs. CON. The treatments of IR-alone, HY + IR, and Sup-HY + IR induce comparable modifications in metabolic pathways. However, in the HY + IR, and Sup-HY + IR groups, the third-instar larvae of B. dorsalis demonstrate significantly fewer changes. Our research suggests that a low-oxygen environment (HY and Sup-HY) might enhance the radiation tolerance in B. dorsalis larvae by stabilizing lipid metabolism pathways at biologically feasible levels. Additionally, our findings indicate that the current phytosanitary IR dose contributes to the effective management of B. dorsalis, without being influenced by radioprotective effects. These results hold significant importance for understanding the biological effects of radiation on B. dorsalis and for developing IR-specific regulatory guidelines under MA environments.

Single NIR laser-activated synergistic low-temperature photothermal therapy and self-oxygenated photodynamic therapy nano-platforms for bacteria infection

The emergence and spread of drug-resistant bacterias with new resistance mechanisms leads to antimicrobial resistance and continues to threaten our ability to treat common infections. Herein, a nano-platform (PB@MnO2@MB@HA) was provided to bring significant bacterial inhibition via win-win photo-combination therapy strategy mediated by a single wavelength. More importantly, with well photothermal conversion efficiency, the nano-platform enables effective low temperature photothermal therapy (below 42 °C), where the heat generated is advantageous for the advancement of photodynamic therapy. Moreover, the addition of manganese dioxide promotes the supply of oxygen and addresses the limitations of low-oxygen environments on photodynamic therapy, further enhancing its therapeutic effect, which in turn compensates for the efficacy of low temperature photothermal. The nano-platform can effectively inhibit the growth of Gram-negative bacteria with an inhibition rate of 99%. In summary, the combined therapy of low-temperature photothermal therapy and self-oxygenated photodynamic therapy synergistically improves the efficacy of bacteria treatment while reducing the side effects associated with two therapy alone. The photo-combination nano-platform triggered by single wavelength near infrared irradiation has significant clinical application potential in overcoming the limitations of monotherapy for bacterial infection.

Metabarcoding reveals unique microbial mat communities and evidence of biogeographic influence in low-oxygen, high-sulfur sinkholes and springs.

High-sulfur, low-oxygen environments formed by underwater sinkholes and springs create unique habitats populated by microbial mat communities. To explore the diversity and biogeography of these mats, samples were collected from three sites in Alpena, Michigan, one site in Monroe, Michigan, and one site in Palm Coast, Florida. Our study investigated previously undescribed eukaryotic diversity in these habitats and further explored their bacterial communities. Mat samples and water parameters were collected from sulfur spring sites during the spring, summer, and fall of 2022. Cyanobacteria and diatoms were cultured from mat subsamples to create a culture-based DNA reference library. Remaining mat samples were used for metabarcoding of the 16S and rbcL regions to explore bacterial and diatom diversity, respectively. Analyses of water chemistry, alpha diversity, and beta diversity articulated a range of high-sulfur, low-oxygen habitats, each with distinct microbial communities. Conductivity, pH, dissolved oxygen, temperature, sulfate, and chloride had significant influences on community composition but did not describe the differences between communities well. Chloride concentration had the strongest correlation with microbial community structure. Mantel tests revealed that biogeography contributed to differences between communities as well. Our results provide novel information on microbial mat composition and present evidence that both local conditions and biogeography influence these unique communities.

Effect of impinging behavior annular jet shielding gas and printing substrate on metal droplet stable ejection

Using coaxial shielding gas for low-oxygen protection in metal droplet-based 3D printing helps to promote flexible production and lightweight manufacturing. However, the presence of the printing substrate causes the shielding gas to exhibit complex annular impinging jet characteristics, making the stability of droplet ejection unpredictable. In the present work, the mechanisms of airflow pattern evolution on droplet formation and metal jet deflection were first revealed by incorporating shielding gas simulations, hydrodynamic modeling, and droplet ejection experiments. An innovative airflow disturbance suppression strategy for metal droplet ejection was proposed, which can remarkably reduce the shielding gas disturbance on droplet printing. Results show that the change in deposition distance leads to a transition between two typical airflow patterns, thus affecting the droplet ejection behavior. When the deposition distance exceeds 2.5 mm, metal jets would be stretched even to a secondary break under airflow pattern 1, accelerating droplets. For the deposition distance below 2.5 mm, metal jet shortening and droplet deceleration would occur under airflow pattern 2, deflecting jet trajectory. The negative airflow effect on droplet ejection could be avoided by controlling the deposition distance to the transition region of two airflow patterns. Furthermore, a ball grid array (BGA) chip ball-mounting and two thin-wall tube printing were realized based on metal droplet ejection in annular impinging jet shielding gas. This work provides theoretical and technical guidance for the stable ejection and accurate printing of metal droplets in an opening low-oxygen environment.

In situ observation on two ‘elusive’ rhodaliid siphonophores (Cnidaria; Hydrozoa; Siphonophora) from the Red Sea, including a potential new species within the genus Archangelopsis

Rhodaliids, a specific family of siphonophores inhabiting the benthic zone, have remained enigmatic due to their rarity and elusive nature. These unique organisms, primarily found in open ocean habitats, exhibit distinctive features and are characterized by complex structures. During the Red Sea Decade Expedition, two rhodaliid specimens were collected at the sea bed at water depths of 438 and 495 meters. Regardless of challenges in specimen preservation, detailed morphological analysis revealed unique characteristics. Genetic analysis, employing the 16S rRNA marker, revealed one specimen closely related to Thermopalia taraxaca, while the taxonomic traits of this specimen suggested the identification of Archangelopsis jagoa. While most of the morphological features of the second specimen were also very similar to A. jagoa, some differences in coloration suggest the discovery of either an unusual colour variant or a potential new species within this genus. Notably, this study reports the first sequencing of A. jagoa. This species demonstrated a capacity to thrive in low-oxygen environments, challenging conventional assumptions about their habitat requirements. Despite difficulties in specimen handling and genetic analysis limitations due to a lack of comprehensive data, this research sheds light on the elusive world of benthic rhodaliids.

The HIF-1α/EGF/EGFR Signaling Pathway Facilitates the Proliferation of Yak Alveolar Type II Epithelial Cells in Hypoxic Conditions.

The yak is a unique creature that thrives in low-oxygen environments, showcasing its adaptability to high-altitude settings with limited oxygen availability due to its unique respiratory system. However, the impact of hypoxia on alveolar type II (AT2) epithelial cell proliferation in yaks remains unexplored. In this study, we investigated the effects of different altitudes on 6-month-old yaks and found an increase in alveolar septa thickness and AT2 cell count in a high-altitude environment characterized by hypoxia. This was accompanied by elevated levels of hypoxia-inducible factor-1α (HIF-1α) and epidermal growth factor receptor (EGFR) expression. Additionally, we observed a significant rise in Ki67-positive cells and apoptotic lung epithelial cells among yaks inhabiting higher altitudes. Our in vitro experiments demonstrated that exposure to hypoxia activated HIF-1α, EGF, and EGFR expression leading to increased proliferation rates among yak AT2 cells. Under normal oxygen conditions, activation of HIF-1α enhanced EGF/EGFR expressions which subsequently stimulated AT2 cell proliferation. Furthermore, activation of EGFR expression under normoxic conditions further promoted AT2 cell proliferation while simultaneously suppressing apoptosis. Conversely, inhibition of EGFR expression under hypoxic conditions had contrasting effects. In summary, hypoxia triggers the proliferation of yak AT2 cells via activation facilitated by the HIF-1α/EGF/EGFR signaling cascade.

The Effects of Altitude Training on the Performance of Open Water Swimmers

Background and Aim: Training in a low-pressure, low-oxygen environment at the plateau is a difficult challenge for athletes who live on the plains all year round. Athletes need to overcome the stimulation of hypoxia to complete training. This paper aims to explore the influence of altitude training on the special quality, technique, and tactics of open-water swimmers. Methods: The method of literature, experiment, and mathematical statistics is adopted, and the experimental method is the main method. (1) The experimental subjects were male athletes with many years of experience in open-water swimming training and competition. (2) Test variable: swimming pool 1500m freestyle speed, swimming pool 50m freestyle speed, open water speed per lap, stroke amplitude, allocation of swimming speed. Results: (1) There was a significant difference between 1500 m swimming speed and open water swimming speed in the experimental group before and after the experiment; (2) The experimental group had a significant difference in 50m swimming speed before and after the experiment, the control group had a significant difference in 50m swimming speed before and after the experiment, and the two groups had a significant difference in 50m swimming speed after the experiment; (3) There was no significant difference in the athletes' swimming speed allocation after the experiment; (4) The stroke amplitude of the experimental group was significantly different after the experiment, and the stroke amplitude of the control group was significantly different after the experiment. Conclusion: Altitude training can improve the athletes' aerobic exercise ability, improve the athletes' backward swimming speed, and improve the athletes' stroke frequency. However, it has some negative effects on the athletes' speed quality and stroke effect.

Application and reduction of a nonlinear hyperelastic wall model capturing ex vivo relationships between fluid pressure, area, and wall thickness in normal and hypertensive murine left pulmonary arteries.

Pulmonary hypertension is a cardiovascular disorder manifested by elevated mean arterial blood pressure (>20 mmHg) together with vessel wall stiffening and thickening due to alterations in collagen, elastin, and smooth muscle cells. Hypoxia-induced (type 3) pulmonary hypertension can be studied in animals exposed to a low oxygen environment for prolonged time periods leading to biomechanical alterations in vessel wall structure. This study introduces a novel approach to formulating a reduced order nonlinear elastic structural wall model for a large pulmonary artery. The model relating blood pressure and area is calibrated using ex vivo measurements of vessel diameter and wall thickness changes, under controlled pressure conditions, in left pulmonary arteries isolated from control and hypertensive mice. A two-layer, hyperelastic, and anisotropic model incorporating residual stresses is formulated using the Holzapfel-Gasser-Ogden model. Complex relations predicting vessel area and wall thickness with increasing blood pressure are derived and calibrated using the data. Sensitivity analysis, parameter estimation, subset selection, and physical plausibility arguments are used to systematically reduce the 16-parameter model to one in which a much smaller subset of identifiable parameters is estimated via solution of an inverse problem. Our final reduced one layer model includes a single set of three elastic moduli. Estimated ranges of these parameters demonstrate that nonlinear stiffening is dominated by elastin in the control animals and by collagen in the hypertensive animals. The pressure-area relation developed in this novel manner has potential impact on one-dimensional fluids network models of vessel wall remodeling in the presence of cardiovascular disease.

Research progress on reproductive system damage caused by high altitude hypoxia.

The high altitude area is characterized by low pressure and hypoxia, and rapidly entering the high altitude area will cause a series of damage to the body. Some studies have shown that hypoxia can cause damage to the reproductive system. In recent years, researchers have been paying attention to the effects of hypoxia on hormone level, ovarian reserve, embryonic development, testicular development, sperm motility level, and have begun to explore its injury mechanism, but its mechanism is not clear. In this paper, the mechanism of hypoxia on the reproductive system is reviewed, which is expected to provide a new idea for solving the problem of the low fertility rate of humans and animals at high altitudes. A comprehensive PubMed search was conducted, selecting all relevant peer-reviewed English papers published before January 2022. Other relevant papers were selected from the list of references. Studies have shown that the complete fertility rate of people living at low altitudes is 7.7, and the complete fertility rate of people living at high altitudes is 4.77, and the hypoxic environment at high altitudes reduces fertility. At the same time, high-altitude, low-oxygen environments are associated with increased infant mortality and post-neonatal mortality. To date, most studies seem to point to a correlation between anoxic exposure at high altitudes and low fertility in humans and animals. Although the molecular mechanisms are not fully understood, the effects of hypoxia at high altitude on hormonal level, ovarian reserve, embryonic development, testicular development, and sperm motility and levels require further research to investigate this complex topic.

Highly active fish in low oxygen environments: vertical movements and behavioural responses of bigeye and yellowfin tunas to oxygen minimum zones in the eastern Pacific Ocean

Oxygen minimum zones in the open ocean are predicted to significantly increase in volume over the coming decades as a result of anthropogenic climatic warming. The resulting reduction in dissolved oxygen (DO) in the pelagic realm is likely to have detrimental impacts on water-breathing organisms, particularly those with higher metabolic rates, such as billfish, tunas, and sharks. However, little is known about how free-living fish respond to low DO environments, and therefore, the effect increasing OMZs will have cannot be predicted reliably. Here, we compare the responses of two active predators (bigeye tuna Thunnus obesus and yellowfin tuna Thunnus albacares) to DO at depth throughout the eastern Pacific Ocean. Using time-series data from 267 tagged tunas (59,910 days) and 3D maps of modelled DO, we find that yellowfin tuna respond to low DO at depth by spending more time in shallower, more oxygenated waters. By contrast, bigeye tuna, which forage at deeper depths well below the thermocline, show fewer changes in their use of the water column. However, we find that bigeye tuna increased the frequency of brief upward vertical excursions they performed by four times when DO at depth was lower, but with no concomitant significant difference in temperature, suggesting that this behaviour is driven in part by the need to re-oxygenate following time spent in hypoxic waters. These findings suggest that increasing OMZs will impact the behaviour of these commercially important species, and it is therefore likely that other water-breathing predators with higher metabolic rates will face similar pressures. A more comprehensive understanding of the effect of shoaling OMZs on pelagic fish vertical habitat use, which may increase their vulnerability to surface fisheries, will be important to obtain if these effects are to be mitigated by future management actions.

The influence of EEG-biofeedback training and Beta waves in normoxia and normobaric hypoxia on the bench press in judo athletes

The aim of this study was to assess the effect of EEG -Biofeedback training in normoxic and normobaric hypoxic conditions on bench press improving in elite judo athletes. The study included 20 male elite judo athletes. Each athlete was a member of the Polish National Team and held either an International Master Class (MM) or National Master Class (M) – purposive selection. The research was carried out over two cycles that differed in the external conditions of EEG biofeedback trainings for both the control and experimental groups. The experimental group underwent training under simulated hypoxic conditions The training sessions for the experimental group were conducted at a simulated altitude of 3000m above sea level. Each cycle of the study comprised of 15 training sessions. The control group's study followed the same frequency, and duration of EEG biofeedback training sessions, and demonstrated an identical pattern to that of the experimental group, but in normoxia conditions. In the discussed cycle conducted in hypoxia, a clear upward dynamics of Beta wave values with a growing trend from session 14 was observed. In normoxia, the variability in the EG group had a significantly less dynamic course. In hypoxia, a clear upward dynamics of the achieved PP values with a growing trend from measurement 14 was observed. In normoxia, the discussed measurement cycle conducted in the CG group showed a lesser upward dynamics of achieved PP values with a growing trend from measurement 10. The overall conclusion from these analyses suggests that EEG biofeedback training in hypoxic conditions may have a beneficial impact on brain activity and improvement in muscle strength and power. These results may be of significant importance for sports training and rehabilitation, especially for individuals engaged in sports disciplines requiring physical exertion in low oxygen environments.

Convergent evolution in high-altitude and marine mammals: Molecular adaptations to pulmonary fibrosis and hypoxia.

High-altitude and marine mammals inhabit distinct ecosystems but share a common challenge: hypoxia. To survive in low-oxygen environments, these species have evolved similar phenotypic pulmonary adaptations, characterized by a high density of elastic fibers. In this study, we explored the molecular mechanisms underlying these adaptations, focusing on pulmonary fibrosis and hypoxia tolerance through comparative genomics and convergent evolution analyses. We observed significant expansions and contractions in certain gene families across both high-altitude and marine mammals, closely associated with processes involved in pulmonary fibrosis. Notably, members of the keratin gene family, such as KRT17 and KRT14, appear to be associated with the development of the dense elastic fiber phenotype observed in the lungs of hypoxia-tolerant mammals. Through selection pressure and amino acid substitution analyses, we identified multiple genes exhibiting convergent accelerated evolution, positive selection, and amino acid substitution in these species, associated with adaptation to hypoxic environments. Specifically, the convergent evolution of ZFP36L1, FN1, and NEDD9 was found to contribute to the high density of elastic fibers in the lungs of both high-altitude and marine mammals, facilitating their hypoxia tolerance. Additionally, we identified convergent amino acid substitutions and gene loss events associated with sperm development, differentiation, and spermatogenesis, such as amino acid substitutions in SLC26A3 and pseudogenization of CFAP47, as confirmed by PCR. These genetic alterations may be linked to changes in the reproductive capabilities of these animals. Overall, this study offers novel perspectives on the genetic and molecular adaptations of high-altitude and marine mammals to hypoxic environments, with a particular emphasis on pulmonary fibrosis.

Effect of Low-Pressure, Low-Oxygen Environment in Plateau on Microcirculation in Acute Pancreatitis

Effect of Low-Pressure, Low-Oxygen Environment in Plateau on Microcirculation in Acute Pancreatitis

MiR-127/3p Inhibits Cell Migration in Lung Adenocarcinoma Under Hypoxic and Normal Oxygen Conditions.

MicroRNAs are small noncoding nucleotides that serve as intracellular and extracellular signaling molecules. A previous collaboration found miR-127/3p circulation in the blood of breast cancer patients correlated with improved patient recovery and prognosis. While this study exclusively focused on breast cancer patients, data mining of the TCGA databases indicated that miR-127/3p may be positively associated with outcomes in other cancer types. In our study, A549 lung adenocarcinoma cells were transfected with miR-127/3p using Cell Block protocols produced by the Cell Biology Education Consortium (CBEC). After transfection, cell migration (scratch/wound healing) assays were used to determine the role miR-127/3p plays in the tumor microenvironment. To mimic and test this environment, transfected cells were incubated in normal oxygen (normoxic) and low oxygen (hypoxic) environments. We found that miR-127/3p inhibited cell migration in both normal oxygen and hypoxic environments. These results help elucidate the role miR-127/3p plays in the prevention of metastasis and further highlight its potential as a positive biomarker.

Efficient hydroxyl radical generation of an activatable phthalocyanine photosensitizer: oligomer higher than monomer and nanoaggregate.

It remains a challenge to develop a single-component organic photosensitizer that efficiently produces hydroxyl radicals (˙OH) without oxygen involvement, especially while maintaining tumor-targeting capability. Herein, we propose an intelligent molecular design strategy whereby a tumor-targeted phthalocyanine is initially ˙OH-free and can be activated by overexpressed β-nicotinamide adenine dinucleotide sodium salt hydrate (NAD(P)H) in hypoxic tumors to efficiently produce ˙OH under light irradiation. Furthermore, the oligomer models based on the phthalocyanine molecules were constructed by a supramolecular regulation strategy, which were in an intermediate state between monomer and nanoaggregate, to achieve enhanced ˙OH generation. The level of NAD(P)H in cancer cells can be exhausted through two pathways, including spontaneous redox and the photocatalytic redox of phthalocyanines. As a result, the in vivo and in vitro assays illustrated that the oligomeric phthalocyanine containing N-O units (OligPcNOB) can specifically target cancer cells and tumor tissue with overexpressing biotin receptors. OligPcNOB exhibited significant photocytotoxicity even in an extremely low oxygen environment and successfully inhibited tumor progression.

Double Bionic Deformable DNA Hydrogel Microneedles Loaded with Extracellular Vesicles to Guide Tissue Regeneration of Diabetes Ulcer Wound

AbstractDue to the high blood glucose and low oxygen environment in diabetes wounds, complex pathological problems arise, such as failure to upregulate healing factors, biological barriers generation, microvascular lesions, and skin neuropathy. Inspired by nature and traditional Chinese paper‐cutting technology, polypeptide deoxyribonucleic acid (DNA) hydrogel microneedles (MNs) (P‐DNA gel MNs) are prepared. The obtained P‐DNA gel MNs possess optimized structure and large deformability, providing excellent clamping, stability, skin penetration, large deformation, tissue adhesion, and antibacterial properties. By incorporating extracellular vesicles (EVs) extracted under hypoxia (H‐EVs‐hypoxia), P‐DNA gel MNs can regulate tissue microenvironment, scavenge free radicals, and reduce inflammation. Notably, P‐DNA gel MNs integration system activates immune regulation and beneficial pathways, promoting neurogenesis and angiogenesis, reducing pathological pain, and achieving high‐quality healing. Overall, P‐DNA gel MNs system presents a fundamental mechanism for clinical transformation in treating diabetes wounds.

Oxygen sensing regulation mechanism of Thauera bacteria in simultaneous nitrogen and phosphorus removal process

Thauera is a genus of denitrifying and phosphorus-accumulating bacteria used for removing nitrogen and phosphorus from wastewater. Dissolved oxygen is one of the main factors affecting denitrification and phosphorus accumulation, and there is currently little research on the oxygen sensing regulation mechanism of Thauera bacteria. We studied denitrifying phosphorus accumulation and gene transcription expression of Thauera sp. RT1901 in anaerobic, microaerobic, and aerobic environments and analyzed the protein structure and regulation mode of two oxygen-sensing systems. The results showed that the strain could achieve the similar denitrification and phosphorus accumulation effect in microaerobic environment as in anaerobic environment. Transcriptome analysis showed that as the oxygen concentration continued to rise, the core carbon and phosphorus metabolism pathways of the strain remained unchanged, and the respiratory system shifted from nitrate respiration to aerobic respiration, thereby inhibiting the denitrification pathway. The fumarate nitrate reduction (FNR) protein and FixLJ two-component system performed oxygen sensing to jointly regulate the expression of respiratory genes. The FNR protein formed a dimer in an anaerobic environment, activating denitrification-related genes and inhibiting cytochrome oxidase-related genes. The FixLJ system conducted signal transduction through phosphorylation in a low oxygen environment and activated the transcription of the fixK gene. The FixK protein has a similar structure and function as the FNR protein but is not directly affected by oxygen. These results reveal the oxygen sensing regulation mechanism of Thauera sp. RT1901 in the denitrification and phosphorus accumulation process, providing new insights for further studying the functions of this genus of bacteria in wastewater treatment systems.

The Evolution of Temperature and Desiccation-Related Protein Families in Tardigrada Reveals a Complex Acquisition of Extremotolerance.

Tardigrada is an ecdysozoan lineage famed for its resilience. Tardigrades can tolerate high doses of radiation, low-oxygen environments, desiccation, and both high and low temperatures under a dormant state called "anhydrobiosis", which is a reversible halt of metabolism upon almost complete desiccation. A large amount of research has focused on the genetic pathways related to these capabilities, and a number of genes have been identified and linked to the extremotolerant response of tardigrades. However, the history of these genes is unclear, and the origins and history of extremotolerant genes within Tardigrada remain a mystery. Here, we generate the first phylogenies of six separate protein families linked with desiccation and radiation tolerance in Tardigrada: cytosolic abundant heat-soluble protein, mitochondrial abundant heat-soluble protein, secretory abundant heat-soluble protein, meiotic recombination 11 homolog, and the newly discovered Echiniscus testudo abundant heat-soluble proteins (alpha and beta). The high number of independent gene duplications found amongst the six gene families studied suggests that tardigrades have a complex history with numerous independent adaptations to cope with aridity within the limnoterrestrial environment. Our results suggest that tardigrades likely transitioned from a marine environment to a limnoterrestrial environment only twice, once in stem Eutardigrada and once in Heterotardigrada, which explains the unique adaptations to anhydrobiosis present in both classes.