Low Organic Loading Research Articles

Long-term simulation of a full-scale EBPR plant with a novel metabolic-ASM model and its use as a diagnostic tool

This study evaluates the predictive capacity of the META-ASM model, a new integrated metabolic activated sludge model, in describing the long-term performance of a full-scale enhanced biological phosphorus removal (EBPR) system that suffers from inconsistent performance. In order to elucidate the causes of EBPR upsets and troubleshoot the process accordingly, the META-ASM model was tested as an operational diagnostic tool in a 1336-day long-term dynamic simulation, while its performance was compared with the ASM-inCTRL model, a version based on the Barker & Dold model. Overall, the predictions obtained with the META-ASM without changing default parameters were more reliable and effective at describing the active biomass of polyphosphate accumulating organisms (PAOs) and the dynamics of their storage polymers. The primary causes of the EBPR upsets were the high aerobic hydraulic retention times (HRTs) and low organic loading rates (OLRs) of the plant, which led to periods of starvation. The impact of these factors on EBPR performance were only identified with the META-ASM model. Furthermore, the first signs of process upsets were predicted by variations in the aerobic PAO maintenance rates, suggesting that the META-ASM model has potential to provide an early warning of process upset. The simulation of a new viable operational strategy indicated that troubleshooting the process could be achieved by reducing the aerated volume by switching off air in the first half of the aeration tank. In this new strategy, the META-ASM model predicted a simultaneous improvement in the biological phosphorus (P) and nitrogen (N) removal due to the enhancement of the hydrolysis and fermentation of the mixed liquor sludge in the new unaerated zone, which increased the availability of volatile fatty acids (VFAs) for PAOs. This study demonstrates that the META-ASM model is a powerful operational diagnostic tool for EBPR systems, capable of predicting and mitigating upsets, optimising performance and evaluating new process designs.

Performance evaluation of a small-scale digester for achieving decentralised management of waste

The performance of a small-scale prototype digestion plant (7.2 m3 working volume) intended for decentralised operation was evaluated considering energy efficiency and technical suitability for biogas valorisation in producing electrical and thermal energy. The digester operated in recirculation mode to enhance organic matter conversion and improve volatile solid degradation. An energy assessment of the process assumed the incorporation of a combined heat and power (CHP) unit. The coefficient of overall performance of the plant for electrical energy (COPel) was 0.95 — this values was estimated at an electrical efficiency of 22.5% and represents the ratio between energy production and consumption — for a methane yield of 360 L/kg VS and an organic loading rate (OLR) of 1.06 g VS/L d. This parameter was slightly lower than the unit thus indicating that the micro-plant was close to attaining self-sufficiency regarding electrical energy use. The temperature increase of the feed to process conditions supposed a significant amount of thermal energy which highly compromised the efficiency when operating at low organic load, thus accounting for more than 80% of the total energy demand of the installation. When the energy assessment of the process was performed at higher OLR of 2.7 g VS/ L d, the resulting COPel value was1.68, demonstrating the feasibility of this configuration for decentralised digestion.

Performance evaluation of microbial fuel cell with sewage wastewater and RO concentrate using composite anode made of Luffa aegyptiaca

AbstractMicrobial Fuel Cells (MFCs) are capable of producing bioelectricity using bacteria as catalysts that break down the organic matter present in wastewater (WW). The present work demonstrates the synergistic effect of Luffa aegyptiaca (LA) combined with graphite plate as anode material on voltage generation as well as pollution removal as chemical oxygen demand (COD). Three different feed combinations of WW and Reverse Osmosis (RO) concentrate that is ‐ Experiment‐1 (75:25), Experiment‐2 (50:50), and Experiment‐3 (25:75) have been considered and their performances are compared with the system without LA in the anode. The result shows that low organic loading rate (OLR) and more RO concentration in feed inlet led to more voltage and normalized energy recovery (NER) that is – 530 mV and 19.51 Whkg−1 COD−1, respectively.

Highly efficient anaerobic co-digestion of food waste and horticultural waste using a three-stage thermophilic bioreactor: Performance evaluation, microbial community analysis, and energy balance assessment

The thermophilic anaerobic co-digestion of food waste and horticultural waste with a three-stage bioreactor was investigated to produce renewable energy, focusing on performance evaluation, microbial community analysis, and energy balance assessment. The results of this study indicated that the average methane yield (0.42 ± 0.06 L/gVS) in the bench-scale three-stage thermophilic bioreactor was approximately 45% and 31% higher than that of the one and two-stage bioreactors, respectively. The average volatile solid (VS) reduction achieved in the bench-scale three-stage thermophilic bioreactor was 63.0 ± 8.2%, which was 61% and 32% higher than that of the one and two-stage bioreactors, respectively. Pyrosequencing analysis of microbial communities in simulated thermophilic three-stage co-digestion experiments showed that bacterial phyla Firmicutes, Thermotogae and Bacteroidetes dominated in all three types of bioreactors. However, significant differences were observed at the genus level of bacterial communities between the one, two and three-stage bioreactors. Indeed, due to selective enrichment, the dominant methanogenic pathways in the three-stage bioreactor shifted from hydrogenotrophic methanogenesis at a low organic loading rate (OLR) to a balance of hydrogenotrophic and acetoclastic pathways at high OLR. Furthermore, assessments of the energy balance and the economic viability of the bench-scale system demonstrated that the three-stage thermophilic co-digestion could be a promising process for the treatment of food waste and horticultural waste as well as for bioenergy recovery at an industrial scale.

Relative electrode size and organic load effects on the energy storage efficiency of microbial electrolysis cells

Microbial electrolysis cells (MECs) have been proposed as a method for storing surplus renewable energy as biofuels, with methane being of particular interest given the existing natural gas infrastructure. However, the effect of the relative size of electrodes and the organic load of the wastewater on the overall performance of MECs designed for methane production remains poorly understood. Here we have explored the use of two MECs containing different relative cathodic and anodic surface areas and compared their methane production from wastewater containing lw (200 ppm) or high (4000 ppm) organic loads. The MEC containing a relatively larger cathode could increase energy storage efficiency by a factor of 1.2 and 3 under high and low organic loads respectively.These results highlight that the relative surface area of the electrodes should be designed according to the rate limiting process. Larger anodes offer more suitable conditions for the attachment of fermentative bacteria, whereas larger cathodes appear to provide more appropriate conditions for methanogens.

Enhanced production of propionic acid through acidic hydrolysis by choice of inoculum

AbstractBACKGROUNDIn this study, the enhancement of propionic acid production from a model feedstock mimicking kitchen waste was investigated. For that purpose, two operational runs of a semicontinuous anaerobic hydrolysis reactor were carried out at pH 6.0 ± 0.1 and mesophilic (30 °C) temperature. Two different types of inocula, a mixed microbial culture selected over 24 months for growth on cellulose and a culture contained in goat cheese were compared.RESULTSThe results show that the goat cheese inoculum was significantly more efficient for propionic acid (PA) production. The highest propionic acid concentration achieved amounted to 139 mmol L−1 at a yield of 23.3 mg g−1 volatile solids (VS), which was 55% greater than what was achieved with the mixed culture. Furthermore, it was observed that propionic acid production was enhanced by a combination of high hydraulic retention time (HRT) with low organic loading rate (OLR), ensuring sufficient time for complete processing of the complex organic substrates. The fermentation could be kept in a stable process of propionic acid production at HRT of 20 days and a rather low OLR of 11.1 g L−1 day−1 VS.CONCLUSIONOur results give a better understanding of PA production in semicontinuous mode, applying optimized process parameters and selecting the adequate microbial community for inoculation. This study provides important information for the improvement of PA production from complex substrates for future industrial application. © 2020 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

Correlation of extracellular polymeric substances and microbial community structure in denitrification biofilm exposed to adverse conditions.

Microbial community may respond to different adverse conditions and result in the variation of extracellular polymeric substances (EPS) in denitrification biofilm; this study discovered the role of EPS in accordance with the analysis of cyclic diguanylate (c-di-GMP) and electron equilibrium (EE) under low organic loading rate, shock organic loading rate and low temperature conditions. Good nitrate removal performance could be achieved under shock organic loading rate and low temperature conditions; however, owing to the low organic loading rate, the carbon source was preferentially utilized for biomass growth. Tightly bound EPS (TB-EPS) contents progressively increased and facilitated cell adhesion and biofilm formation. The stable TB protein (TB-PN) content in TB-EPS built a cross-linked network to maintain internal biofilm structure and led to the rapid biosynthesis of polysaccharides, which could further enhance microbial adhesion and improve nitrate removal. C-di-GMP played an important role in biomass retention and biofilm formation, based on the correlation analysis of c-di-GMP and EPS. TB polysaccharide (TB-PS) contents presented a significant positive correlation with c-di-GMP content, microbial adhesion and biofilm stabilization was further enhanced through c-di-GMP regulation. In addition, a remarkable negative correlation between electron deletion rate (EDR) and TB-PN and TB-PS was discovered, and TB-PS was required to serve as energy source to enhance denitrification according to EE analysis. Surprisingly, dynamic microbial community was observed due to the drastic community succession under low temperature conditions, and the discrepancy between the dominant species for denitrification was found under shock organic loading rate and low temperature conditions. The notable increase in bacterial strains Simlicispira, Pseudomonas and Chryseobacterium was conducive to biofilm formation and denitrification under shock organic loading rate, while Dechloromonas and Zoogloea dramatically enriched for nitrate removal under low temperature conditions. The high abundance of Dechloromonas improved the secretion of EPS through the downstream signal transduction, and the c-di-GMP conserved in Pseudomonas concurrently facilitated to enhance exopolysaccharide production to shock organic loading rate and low temperature conditions.

Effects of Selected Pharmaceuticals on Freshwater Algal Biofilms in Different Organic Loads

The growth pattern of different algal biofilms investigated in the presence of Ibuprofen and Benzoyl Metronidazole in batch reactors at varying organic loads. Study suggests that the inhibition of algal growth is correlated to organic loads in water rather than the concentrations of PhCs (Pharmaceuticals). Mortality of algae was recorded in BG11 media with binary mixtures of PhCs and no growth inhibition was monitored in the activated sludge with PhCs. These outcomes indicate that the presence of PhCs as pollutants in drinking water have severe effect on algal biofilms due to low organic load thus can alter the aquatic ecosystem. Additionally, PhCs might not adversely hinder the growth of microbes in WWTP (Wastewater Treatment Plants) or in waters with high organic loads. Nevertheless, inference of these results to a full-scale WWTP need more evidence through extensive studies using variable organic substrates and PhCs with numerous ranges of organic loads. These findings suggest that PhCs as water contaminant are able to shift microbial ecology in water bodies.

A high-rate anaerobic biofilm reactor for biomethane recovery from source-separated blackwater at ambient temperature.

Anaerobic bioreactors for source-separated blackwater are mostly operated at low organic loading rates (OLRs) due to low biodegradability and the potential of ammonia inhibition. In this study, an anaerobic biofilm reactor having conductive carbon fibers as the media was investigated for the high-rate treatment of blackwater collected from vacuum toilets. The bioreactor was operated at different OLRs ranged from 0.77 to 3.01g COD/L-d in four stages for a total operating period of~250days. With the increase of OLRs, the specific methane production rate increased from 105.3 to 304.6ml/L-d with high methane content in biogas (75.5%-83%). The maximum methane yield was achieved at hydraulic retention time (HRT) of 15days. Highest organics and suspended solids removal (80%-83%) were achieved at 20-days HRT, while increased OLRs resulted in diminished removal efficiencies. The state variables, including pH, total ammonia nitrogen, short-chain volatile fatty acids, and soluble chemical oxygen demand, indicated the system had a great capability to withstand the high OLRs. Microbial community analysis revealed that the high performance might be attributed to direct interspecies electron transfer (DIET) facilitated by potentially electroactive bacteria (e.g., Syntrophomonas, Clostridium) and electrotrophic archaea (e.g., Methanosaeta and Methanosarcina species) enriched on the carbon fibers. PRACTITIONER POINTS: An anaerobic biofilm reactor was investigated for biomethane recovery from source-separated blackwater. Conductive carbon fibers were utilized as the media to stimulate enrichment of potentially electroactive methanogenic communities. The bioreactor was operated at ambient temperature for over 250days. High methane production rate and high-quality biogas were achieved at OLRs ranged from 0.77 to 3.01g COD/L-d. Microbial community analysis suggested direct interspecies electron transfer (DIET) between specific electroactive bacteria and electrotrophic archaea.

Impact of low loading on digestion of the mechanically-separated organic fraction of municipal solid waste

Changing waste management practice, introduction of new technologies, and population demographics and behaviour will impact on both quantity and composition of future waste streams. Laboratory-scale anaerobic digestion of the mechanically-separated organic fraction of municipal solid waste (ms-OFMSW) was carried out at relatively low organic loading rates (OLR), and results analysed using an energy modelling tool. Thermophilic operation with water addition and liquor recycle was compared to co-digestion with dilution water replaced by sewage sludge digestate (SSD); thermophilic and mesophilic mono-digestion were also tested at low OLR. All thermophilic conditions showed stable operation, with specific methane production (SMP) from 0.203 to 0.296 m3 CH4 kg−1 volatile solids (VS). SSD addition increased biogas production by ~20% and there was evidence of further hydrolysis and degradation of the SSD. Long-term operation at 1 kg VS m−3 day−1 had no adverse effect except in mesophilic conditions where SMP was lower at 0.256 m3 CH4 kg−1 VS and stability was reduced, especially during OLR increases. This was probably due to low total ammonia nitrogen, which stabilised at ~0.2 g N kg−1 and limited the buffering capacity. Energy analysis showed thermophilic operation at OLR 2 g VS L−1 day−1 gave 42% of the theoretical methane potential and 38% of the higher heating value, reducing to 37% and 34% respectively in mesophilic conditions. Scenario modelling indicated that under low ms-OFMSW load even an energy-depleted co-substrate such as SSD could contribute to the energy balance, and would be a better diluent than water due to its nutrient and buffering capacity.

Upgrading industrial effluent for agricultural reuse: effects of digestate concentration and wood vinegar dosage on biosynthesis of plant growth promotor.

Emphasis on water reuse in agricultural sector receives a renewed interest to close the loop in circular economy, especially in dry and water-stressed regions. In this work, wastewater from cooperative smoked sheet rubber factory and the effluent (digestate) from its treatment system (anaerobic digester) were used as medium to grow purple non-sulfur bacteria (PNSB), Rhodopseudomonas palustris strain PP803, with wood vinegar supplement at mid-log growth phase to stimulate the release of 5-aminolevulinic acid (ALA), a plant growth promotor. Wastewater-to-digestate ratios (D:W) represented by soluble chemical oxygen demand (SCOD) were found to influence both the growth of R. palustris and synthesis of ALA. The highest ALA release of 16.02 ± 0.75μM and the biomass accumulation of 1302 ± 78mg/L were obtained from the medium SCOD of 4953mg/L. Although retarding biomass accumulation by 28-36%, wood vinegar (WV) addition was proven to improve ALA release by 40%. Result suggested that SCOD of 3438mg/L (75:25 D:W) contained sufficient carbon source for PNSB growth and was chosen to subsequently run the photo-bioreactor (PBR) to sustain R. palustris PP803 cells production. In continuous PBR operation, PNSB proliferation suffered from the low organic concentration in PBR at low organic loading. An organic loading increase to 1.21gCOD/Lday was found to attain highest biomass concentration and longest PNSB dominant period over microalgea. In this study, a real-time monitoring protocol of PNSB and microalgae was specifically developed based on image color analysis at acceptable accuracy (R2 = 0.94). In the final assay, verification of the PBR-grown inoculant was conducted and ALA release efficiency was discussed under various wood vinegar dosages and dosing frequencies. This work has advanced our understandings closer to practical field application.

Efficient carbon, nitrogen and phosphorus removal from low C/N real domestic wastewater with aerobic granular sludge

This work reports on simultaneous nitrification, denitrification and phosphorus removal treating real domestic wastewater with low carbon/nitrogen (C/N) ratio by aerobic granular sludge (AGS). Operations at high sludge retention time (SRT = 61 ± 24 days) resulted in low biomass yield per chemical oxygen demand removed (CODrem) (0.21 ± 0.01 gCODx/gCODrem), lower COD demand for denitrification as well as high effluent quality in terms of total suspended solids (TSS) (22 ± 7 mgTSS/L). The average ratio between the biodegradable soluble COD stored anaerobically as polyhydroxyalkanoates (PHAs) and the N removed was 3.1 ± 0.6 gCODsto/gNrem, suggesting that nitrification/denitrification occurred partly via the nitrite pathway. Results revealed that stable AGS process with high C/N/P removal efficiency of 84/71/96% can be obtained besides a low organic loading rate (0.43 ± 0.11 g COD/L/d) and influent C/N ratio (3.8 ± 1.6 g/g), resulting in a high effluent quality characterized by 25 ± 6 mg sCOD/L, 0.09 ± 0.07 mgPO4-P/L, 9 ± 2 mgTIN/L (10 ± 2 mgTN/L) and 22 ± 7 mgTSS/L.

Sustainable design of large wastewater treatment plants considering multi-criteria decision analysis and stakeholders’ involvement

The typical treatment scheme of a large municipal wastewater treatment plant (LWWTP) is almost always the result of design based on technical and economic criteria. Once a threshold in terms of population equivalent (PE) is reached, it is possible that additional sludge thermal treatment might be required. Aspects such as greenhouse gas (GHG) emissions and land use for the construction of the WWTP or the service landfill are considered marginal in current design practice; in a world that requires increasingly attention to the environment, these criteria cannot be ignored when defining the treatment scheme of a LWWTP. With the intent of providing a sustainable approach to design, this study aims to identify the best treatment scheme for a LWWTP with a 720,000 PE size. Methodologically, the study involves the development of an approach based on multi-criteria decision analysis (MCDA). Six alternative treatment schemes were considered; two simplified schemes, without primary sedimentation, with extended aeration activated sludge processes and aerobic sludge stabilization; four full schemes, with primary sedimentation and anaerobic sludge digestion. Some schemes differ for the organic loading rate applied; others for the use of sludge incineration. Subsequently, six evaluation criteria (ECs) such as GHG emissions, electricity consumption, running costs, WWTP planimetric area, surface for the service landfill, as well as WWTP as biorefinery have been considered. The weighting of the ECs involved the participation of the main stakeholders in such a decision-making process, following a bottom-up approach. The resolution of the MCDA problem allowed the identification of the full scheme based on primary sedimentation, biological activated sludge at low organic load (0.210 kgBOD5/kgVSS/d) and anaerobic sludge digestion as the best solution. The sensitivity analysis, able to indirectly take into account the multitude of decision makers involved, allowed corroborating the results. The obtained treatment scheme was different from that generally adopted in current design practice for LWWTPs.

Microbial community similarity and dissimilarity inside and across full-scale activated sludge processes for simultaneous nitrification and denitrification.

Simultaneous nitrification and denitrification under low dissolved oxygen conditions is an energy-saving modification of the activated sludge process to achieve efficient nitrogen removal. Geographically distinct full-scale treatment plants are excellent platforms to address the links of microbial community with operating parameters. Mixed liquor samples were collected from a sequencing batch reactor plant, oxidation ditch plant, and step-feed activated sludge plant. Next-Generation Sequencing of the samples showed that the microbial communities were similar at the phylum level among the plants, being dominated by Proteobacteria. Microbial composition of functional groups was similar between the react fill and react phases of the sequencing batch reactors, among four sequencing batch reactors, and among four oxidation ditches. Nitrospira was the only identified genus of autotropic nitrifying bacteria with a relative abundance of 2.2-2.5% in the oxidation ditches and 0.4-0.7% at the other plants. Heterotrophic nitrifying-aerobic denitrifying bacteria were dominated by Dechloromonas with a relative abundance of 0.4-1.0%. Microbial community composition and nitrogen removal mechanisms were related to overall level and local zonation of dissolved oxygen, mixed liquor suspended solids concentration, nitrogen and organic loadings, and solids retention time. Low dissolved oxygen and low organic and nitrogen loadings favored growth of Nitrospira.

Food waste leachate treatment using an Upflow Anaerobic Sludge Bed (UASB): Effect of conductive material dosage under low and high organic loads

In this study, the performance of UASB for treating food waste leachate was investigated, with the objective of studying the effect of conductive material on anaerobic digestion (AD) enhancement at two organic loads. Conductive and control materials (i.e. graphite and glass) were first compared for their surface porosity then dosed in UASB for side-by-side comparison of the corresponding AD performance. In the first phase (organic load of 2660 mg-COD/L), compared to glass-added UASB, 29.5% reduction of effluent COD was observed in graphite-added UASB, however, only a little biogas enhancement (2.3%) was achieved. In the second phase (organic load of 4140 mg-COD/L), the results show that it could promote better AD enhancement in graphite-added UASB, where 36% effluent COD and 38% biogas production enhancement were simultaneously observed. The overall results support that utilization of conductive material is a viable approach for enhancing biogas production in UASB, especially for high organic loads.

Nitrogen oxidation consortia dynamics influence the performance of full-scale rotating biological contactors

Ammonia oxidising microorganisms (AOM) play an important role in ammonia removal in wastewater treatment works (WWTW) including rotating biological contactors (RBCs). Environmental factors within RBCs are known to impact the performance of key AOM, such that only some operational RBCs have shown ability for elevated ammonia removal. In this work, long-term treatment performance of seven full-scale RBC systems along with the structure and abundance of the ammonia oxidising bacteria (AOB) and archaea (AOA) communities within microbial biofilms were examined. Long term data showed the dominance of AOB in most RBCs, although two RBCs had demonstrable shift toward an AOA dominated AOM community. Next Generation Sequencing of the 16S rRNA gene revealed diverse evolutionary ancestry of AOB from RBC biofilms while nitrite-oxidising bacteria (NOBs) were similar to reference databases. AOA were more abundant in the biofilms subject to lower organic loading and higher oxygen concentration found at the distal end of RBC systems. Modelling revealed a distinct nitrogen cycling community present within high performing RBCs, linked to efficient control of RBC process variables (retention time, organic loading and oxygen concentration). We present a novel template for enhancing the resilience of RBC systems through microbial community analysis which can guide future strategies for more effective ammonia removal. To best of the author’s knowledge, this is the first comparative study reporting the use of next generation sequencing data on microbial biofilms from RBCs to inform effluent quality of small WWTW.

Application of in-situ H2-assisted biogas upgrading in high-rate anaerobic wastewater treatment

The H2-assisted biogas upgrading approach has recently attracted much interest as a low-cost and environmentally friendly alternative to commonly used ex-situ/ physiochemical biogas upgrading techniques. However, most studies conducted to date have been limited to anaerobic solid-waste treatment characterized by flocculant sludge and low organic loading rates (OLR). In an attempt to expand its application to high-rate anaerobic wastewater treatment, an innovative two-stage up-flow anaerobic sludge blanket reactor system was employed using anaerobic granular sludge. We found that the CH4 content of product gas was consistently >90% and that H2 and CO2 concentrations stayed below 5%, even when OLR was increased from 1 to 5 g L−1 d−1 and H2 feeding rates were increased from 0.13 to 0.63 g L−1 d−1. We were also able to show that CO (5–10%) in H2-rich syngas didn’t inhibit methanogenesis or had significant impact on microbial community structure, suggesting that H2-assisted biogas upgrading with H2-rich syngas is feasible.

Metabolomics Study of Subsurface Wastewater Infiltration System Under Fluctuation of Organic Load.

Subsurface Wastewater Infiltration System (SWIS) is a sewage ecological treatment technology with low investment, energy consumption, and operating cost. SWIS soil contains a large variety of microorganisms. The metabolic process and production of microorganisms are an important basis for qualitatively describing the process of pollutant removal. In order to discover the microbial decontamination pathways in SWIS, the metabolic profiles of soil microorganisms in SWIS were analyzed by UPLC-MS. Partial least squares-discriminant analysis (PLS-DA)and principal component analysis (PCA) pattern recognition methods were used to classify the samples. According to the model's variable importance factor (VIP value), potential biomarkers were screened and biological information contained in the metabolites was also analyzed. The correlation between metabolites and environmental factors was explored by RDA analysis. In total, 230 differential metabolites with VIP value greater than 1.5 were screened out when the influent organic load fluctuated at 250mgL-1, 400mgL-1, and 500mgL-1. After identifying and screening, 35 differential metabolites were identified and used to further analyze the metabolic pathway. It turns out that microbial metabolites in SWIS were mainly glycosides, fatty acids, amino acids, pigments, diterpenoids, and some polymers under medium and high organic loading conditions. At low organic load, the microbial metabolites in SWIS were mainly ketones, alcohols, and esters.

Anaerobic digestion of mixed urban biowaste: The microbial community shift towards stability

Anaerobic digestion is applied worldwide to treat food waste (FW) with the aim of obtaining renewable bioenergy by exploiting the methane gas produced. However, there are several problems in practical applications, primarily due to system instability. Although exhaustive knowledge regarding anaerobic microbial community composition has been established, few studies have investigated long-term correlations between microbial consortia, operative conditions and feedstock characteristics. Here, microbial community shifts as a response to feedstock variations were investigated in long-term semi-continuous systems, which were evaluated by an in situ cell detection method and 16S rRNA gene amplicon sequencing. FW digestion showed progressive system instability caused by the inhibition of methanogens, which resulted in volatile fatty acid accumulation and process failure at the low organic loading rate (OLR). Conversely, by co-digesting FW with waste-activated sludge (WAS), a stable process with methane yields of up to 0.27 Nm3 kg−1VSfed for OLR = 1.7 gVS L−1d−1 was achieved. This stabilizing effect was not related to the buffering capacity of WAS, but to its capacity to avoid volatile fatty acid accumulation and falls in pH by overcoming methanogenic activity inhibition. WAS addition promoted the establishment of a stable and active archaeal population in anaerobic co-digestion (AcoD) reactors. The continuous supply of trace elements together with the seeding of microbial functional groups were the main drivers that positively affected process stability.

Biogranulation and Physical Properties of Aerobic Granules in Reactors at Low Organic Loading Rate and with Powdered Ceramsite Added

Biogranulation and Physical Properties of Aerobic Granules in Reactors at Low Organic Loading Rate and with Powdered Ceramsite Added