Sulfur and O isotope analyses of dissolved SO 4 were used to constrain a hydrogeological model for the area overlying the Gorleben–Rambow Salt Structure, Northern Germany. Samples were collected from 80 wells screened at different depth-intervals. The study area consists of a set of two vertically stacked aquifer systems. Generally, the isotope data show a good spatial correlation, outlining well-defined groundwater zones containing SO 4 of characteristic isotopic composition. Highly saline waters from deeper parts of the lower aquifer system are characterized by rather constant SO 4 isotopic compositions, which are typical of Permian Zechstein evaporites (δ 34 S=9.6–11.9‰; δ 18 O=9.5–12.1‰). Above this is a transition zone containing ground waters of intermediate salinity and slightly higher isotopic values (average δ 34 S=16.6‰; δ 18 O=15.3‰). The confined groundwater horizon on the top of the lower aquifer system below the low permeable Hamburg Clays is low in total dissolved solids and is characterized by an extreme 34S enrichment (average δ 34 S=39.1‰; δ 18 O=18.4‰), suggesting that bacterially mediated SO 4 reduction is a dominant geochemical process in this zone. Two areas of distinct isotopic composition can be identified in the shallow ground water horizons of the upper hydrogeological system. Sulfate in groundwaters adjacent to the river Elbe and Löcknitz has a typical meteoric isotopic signature (δ 34 S=5.2‰; δ 18 O=8.2‰), whereas the central part of the area is characterized by more elevated isotopic ratios (δ 34 S=12.7‰; δ 18 O=15.6‰). The two major SO 4 pools in the area are represented by Permian seawater SO 4 and a SO 4 of meteoric origin that has been mixed with SO 4 resulting from the oxidation of pyrite. It is suggested that the S-isotope compositions observed reflect the nature of the SO 4 source that have been modified to various extent by bacterial SO 4 reduction. Groundwaters with transitional salinity have resulted from mixing between brines and low-mineralized waters affected by bacterial SO 4 reduction.
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