Low Phosphorus Levels Research Articles

Experimental investigation of simultaneous nitrification-denitrification and phosphorus removal in pilot-scale sequencing batch moving bed biofilm reactors (SB-MBBRs)

Sequencing batch moving bed biofilm reactors have been widely used in commercial wastewater treatment facilities for organic carbon and nitrogen removal. However, these reactors can remove low phosphorus (P) levels. Therefore, this study investigated the potential of SB-MBBRs for maximizing simultaneous nitrification-denitrification and P removal (SNDPR) potential from P-rich municipal wastewater impacted by industrial discharges. A series of experiments were carried out to investigate the effect of external volatile fatty acid (VFA) dosing, airflow rate, and temperature on SNDPR using pilot-scale SBMBBRs. Stable and robust SNDPR was achieved with an optimum acetic acid supply of 150 mg SCOD/L, at 20 oC and 2.5 L air/min. A low airflow rate (AFR) and high-temperature conditions affected P release and uptake kinetics. Efficient PHA storage, dissolved oxygen (DO) transfer (outer layer), DO diffusion limitation (inner layer) of biofilm, and conversion of NH4-N to NO2-N/NO3-N enhanced SNDPR in the two pilot SB-MBBRs.

Assessment of salt-affected soil in selected rice irrigation schemes in Tanzania: understanding salt types for optimizing management approaches

Salt-affected soils have serious implications for agricultural land quality and productivity, leading to a reduction in the net cultivable area available for food production. This issue has emerged as one of the foremost global challenges in recent years, impacting both food security and environmental sustainability. This research focuses on soil characterizations conducted in three irrigation schemes to understand the types, distribution, and nature of salt-affected soils. Auger observations served as the basic methodology for soil unit delineation in this study. Twelve representative soil pits with a depth of more than 160 cm were described, and undisturbed and disturbed samples were collected and analyzed for physical and chemical properties. The schemes were characterized by low levels of available phosphorus, organic carbon, and total nitrogen, as well as variations in exchangeable bases. Furthermore, 37.5% of the analyzed horizons highlighted the presence of salt-affected soils. Sodium was the predominant cation, followed by magnesium, potassium, and calcium. Likewise, HCO3− was the dominant anion, followed by Cl− and SO42−. The results of this study reveal the existence of two types of salt-affected soil. Uturo irrigation scheme had sodic soils, while the Ndungu irrigation scheme had saline-sodic soils. Additionally, both sodic and saline-sodic soils have been discovered in the Mawala irrigation scheme. The root cause of this challenge is inherent soil conditions, exacerbated by inadequate drainage infrastructure. Therefore, measures such as the application of gypsum followed by salt leaching, improving soil drainage by incorporating organic matter, improving drainage infrastructure, and using rice-tolerant varieties are recommended to mitigate salt stress and improve soil fertility. It is imperative to establish a robust framework for continuous monitoring and regular assessment of soil health to enable timely interventions and informed decisions for sustainable agricultural management.

Dependence of evolution of Cyanobacteria superiority on temperature and nutrient use efficiency in a meso-eutrophic plateau lake

Algal blooms in lakes have been a challenging environmental issue globally under the dual influence of human activity and climate change. Considerable progress has been made in the study of phytoplankton dynamics in lakes; The long-term in situ evolution of dominant bloom-forming cyanobacteria in meso-eutrophic plateau lakes, however, lacks systematic research. Here, the monthly parameters from 12 sampling sites during the period of 1997–2022 were utilized to investigate the underlying mechanisms driving the superiority of bloom-forming cyanobacteria in Erhai, a representative meso-eutrophic plateau lake. The findings indicate that global warming will intensify the risk of cynaobacteria blooms, prolong Microcystis blooms in autumn to winter or even into the following year, and increase the superiority of filamentous Planktothrix and Cylindrospermum in summer and autumn. High RUETN (1.52 Biomass/TN, 0.95–3.04 times higher than other species) under N limitation (TN < 0.5 mg/L, TN/TP < 22.6) in the meso-eutrophic Lake Erhai facilitates the superiority of Dolichospermum. High RUETP (43.8 Biomass/TP, 2.1–10.2 times higher than others) in TP of 0.03–0.05 mg/L promotes the superiority of Planktothrix and Cylindrospermum. We provided a novel insight into the formation of Planktothrix and Cylindrospermum superiority in meso-eutrophic plateau lake with low TP (0.005–0.07 mg/L), which is mainly influenced by warming, high RUETP and their vertical migration characteristics. Therefore, we posit that although the obvious improvement of lake water quality is not directly proportional to the control efficacy of cyanobacterial blooms, the evolutionary shift in cyanobacteria population structure from Microcystis, which thrives under high nitrogen and phosphorus conditions, to filamentous cyanobacteria adapted to low nitrogen and phosphorus levels may serve as a significant indicator of water quality amelioration. Therefore, we suggest that the risk of filamentous cyanobacteria blooms in the meso-eutrophic plateau lake should be given attention, particularly in light of improving water quality and global warming, to ensure drinking water safety.

The Influence of Chemical Fertilizers on the Effectiveness of Biochar in Mitigating Cadmium Mobility in Soil

The focus of this study was to explore how various fertilizers influence the movement of cadmium in soil treated with biochar. The research utilized a strong acid sandy loam soil from Chanthaburi Province, naturally rich in organic matter with moderate cation exchange capacity and low nitrogen, potassium, phosphorus, sulfate, salinity and chloride levels. The soil was purposely contaminated with 50 mg kg-1 of cadmium and treated with biochar derived from water hyacinth through pyrolysis at 450°C for an hour. This biochar displayed moderate alkalinity, high organic matter, phosphorus, potassium, and cation exchange capacity, but low nitrogen content. Analytical techniques like Scanning Electron Microscopy and Fourier-Transform Infrared Spectroscopy were employed to study the surface characteristics of the biochar. The cadmium adding soil was blended with 10% biochar and various chemical fertilizers at a 0.04% ratio. Diethylenetriamine pentaacetate (DTPA) extraction was used to assess the bioavailability of cadmium to plants in the soil, while sequential extraction was conducted to identify the different forms of cadmium present in the soil. The study revealed distinct effects of different fertilizers on cadmium mobility. Fertilizers like 46-0-0 and 0-3-0 caused a transformation of cadmium from stable to less stable forms, increasing the bioavailability of cadmium to plants. Conversely, fertilizers such as 15-15-15, 0-0-50, 0-0-60, and 0-52-34 shifted cadmium from less stable to more stable forms, resulting in decreased cadmium extracted by DTPA. Other fertilizers showed no significant impact on cadmium mobility in the soil. A stepwise linear regression analysis highlighted that nitrogen content, potassium content, and electrical conductivity were influential factors affecting cadmium mobility.

Age-Related Changes in Clinical and Analytical Variables in Chronic Hemodialyzed Patients.

Worldwide, the number of elderly individuals receiving chronic hemodialysis is rising. The aim of our study was to evaluate several clinical and analytical biomarkers in chronically dialyzed patients and analyze how they change with age. A cross-sectional study was performed by evaluating 289 end-stage renal disease patients undergoing dialysis. We evaluated the hemogram, adipokines, the lipid profile, and several markers related to inflammation, endothelial function/fibrinolysis, nutrition, iron metabolism, and cardiac and renal fibrosis. Clinical data and dialysis efficacy parameters were obtained from all patients. The relationships between studied biomarkers and age were assessed by a statistical comparison between younger (adults with age < 65 years) and older (age ≥ 65 years) patients and by performing regression analysis. Participants presented a mean age of 68.7 years (±13.6), with 66.8% (n = 193) being classified as older. Compared to younger patients, older patients presented the following: (a) significantly lower values of diastolic blood pressure (DBP) and ultrafiltration volume; (b) lower levels of phosphorus, uric acid, creatinine, and albumin; and (c) higher circulating concentrations of tissue-type plasminogen activator (tPA), D-dimer, interleukin-6, leptin, N-terminal pro B-type natriuretic peptide, and tissue inhibitor of metalloproteinase-1. In the multiple linear regression analysis, DBP values, tPA, phosphorus, and D-dimer levels were independently associated with the age of patients (standardized betas: -0.407, 0.272, -0.230, and 0.197, respectively; p < 0.001 for all), demonstrating relevant changes in biomarkers with increasing age at cardiovascular and nutritional levels. These findings seem to result from crosstalk mechanisms between aging and chronic kidney disease.

Community assembly of organisms regulates soil microbial functional potential through dual mechanisms.

Unraveling the influence of community assembly processes on soil ecosystem functioning presents a major challenge in the field of theoretical ecology, as it has received limited attention. Here, we used a series of long-term experiments spanning over 25 years to explore the assembly processes of bacterial, fungal, protist, and nematode communities using high-throughput sequencing. We characterized the soil microbial functional potential by the abundance of microbial genes associated with carbon, nitrogen, phosphorus, and sulfur cycling using GeoChip-based functional gene profiling, and determined how the assembly processes of organism groups regulate soil microbial functional potential through community diversity and network stability. Our results indicated that balanced fertilization (NPK) treatment improved the stochastic assembly of bacterial, fungal, and protist communities compared to phosphorus-deficient fertilization (NK) treatment. However, there was a nonsignificant increase in the normalized stochasticity ratio of the nematode community in response to fertilization across sites. Our findings emphasized that soil environmental factors influenced the assembly processes of the biotic community, which regulated soil microbial functional potential through dual mechanisms. One mechanism indicated that the high phosphorus levels and low soil nutrient stoichiometry may increase the stochasticity of bacterial, fungal, and protist communities and the determinism of the nematode community under NPK treatment, ultimately enhancing soil microbial functional potential by reinforcing the network stability of the biotic community. The other mechanism indicated that the low phosphorus levels and high soil nutrient stoichiometry may increase the stochastic process of the bacterial community and the determinism of the fungal, protist, and nematode communities under NK treatment, thereby enhancing soil microbial functional potential by improving the β-diversity of the biotic community. Taken together, these results provide valuable insights into the mechanisms underlying the assembly processes of the biotic community that regulate ecosystem functioning.

Influence of plant species, mycorrhizal inoculant, and soil phosphorus level on arbuscular mycorrhizal communities in onion and carrot roots.

Arbuscular mycorrhizal fungi (AMF) are ancient and ecologically important symbionts that colonize plant roots. These symbionts assist in the uptake of water and nutrients, particularly phosphorus, from the soil. This important role has led to the development of AMF inoculants for use as biofertilizers in agriculture. Commercial mycorrhizal inoculants are increasingly popular to produce onion and carrot, but their specific effects on native mycorrhizal communities under field conditions are not known. Furthermore, adequate availability of nutrients in soils, specifically phosphorus, can reduce the diversity and abundance of AMF communities in the roots. The type of crop grown can also influence the composition of AMF communities colonizing the plant roots. This study aimed to investigate how AMF inoculants, soil phosphorus levels, and plant species influence the diversity of AMF communities that colonize the roots of onion and carrot plants. Field trials were conducted on high organic matter (muck) soil in the Holland Marsh, Ontario, Canada. The treatments included AMF-coated seeds (three to five propagules of Rhizophagus irregularis per seed) and non-treated onion and carrot seeds grown in soil with low (~46 ppm) and high (~78 ppm) phosphorus levels. The mycorrhizal communities colonizing the onion and carrot roots were identified by Illumina sequencing. Five genera, Diversispora, Claroideoglomus, Funneliformis, Rhizophagus, and Glomus, were identified in roots of both plant species. AMF communities colonizing carrot roots were more diverse and richer than those colonizing onion roots. Diversispora and Funneliformis had a 1.3-fold and 2.9-fold greater abundance, respectively, in onion roots compared to carrots. Claroideoglomus was 1.4-fold more abundant in carrot roots than in onions. Inoculation with R. irregularis increased the abundance and richness of Rhizophagus in AMF communities of onion roots but not in carrot roots. The soil phosphorus level had no effect on the richness and diversity of AMF in the roots of either crop. In summary, AMF inoculant and soil phosphorus levels influenced the composition of AMF communities colonizing the roots of onion and carrot plants, but the effects varied between plant species.

Effect of Mongolian medicine alcohol spray therapy on fracture healing in rabbits

Purpose: To evaluate the healing effect and mechanism of Mongolian medicine alcohol spray therapy on fracture healing process in rabbits.&#x0D; Methods: Thirty New Zealand white rabbits were randomly divided into normal, fracture, and treatment groups, with 10 rabbits in each group. Fracture and treatment groups underwent bone-fracture modeling, with the latter receiving daily alcohol spraying and stroking for six weeks. Serum calcium and phosphorus were measured using an automatic biochemical analyzer. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of BMP-2, ALP, and TGF-β. Biomechanical testing was performed to assess callus properties, and real-time polymerase chain reaction (PCR) and western blot were performed to assess BMP-2, Runx2, and p-Smad1/5 expressions in callus.&#x0D; Results: There were low serum calcium, phosphorus, BMP-2, ALP, and TGF-β levels in fracture and treatment groups, compared to normal group. Similarly, treatment group showed significantly increased levels of serum calcium, phosphorus, bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), and transforming growth factor-β (TGF-β) (p &lt; 0.05) compared to fracture group. Biomechanical testing revealed that maximum load, stiffness, and deflection of callus in the fracture and treatment groups were significantly lower compared to normal group (p &lt; 0.05). expressions of BMP-2, Runx2, and p-Smad1/5 mRNA in the callus significantly decreased in both fracture and treatment groups compared to normal group (p &lt; 0.05). However, treatment group showed significantly increased expression levels compared to fracture group (p &lt; 0.05).&#x0D; Conclusion: Mongolian medicine alcohol spray therapy promotes fracture healing in rabbits and increases maximum load of the callus, possibly through upregulation of the BMP-2 signaling pathway. Further research is necessary to confirm the exact mechanism by which alcohol spray therapy regulates BMP-2 signaling pathway.

PEMANFAATAN KOMPOS DAN MIKORIZA UNTUK MEMPERBAIKI KESUBURAN TANAH, PERTUMBUHAN SERTA PRODUKSI TANAMAN JAGUNG MANIS (Zea mays saccharata)

Inceptisols dominate Indonesia's land area; therefore, future utilization needs to be maximized, especially land that has undergone extensive tillage continuously. Inceptisols have low levels of essential nutrients, especially nitrogen, phosphorus, and potassium. One of the efforts that can be made to improve nutrient content is the application of compost. This study aimed to investigate the effect of compost combined with mycorrhiza on total N, available P, exchangeable K, and soil organic C, as well as the growth and yield of sweet corn. The experimental design used in this study was a completely randomized design consisting of five treatments with three replications. Each observation parameter was analyzed using the F test at the 5% level. Parameters that significantly affected the F test were followed by the DMRT test at the 5% level. All observed data were tested for correlation using SPSS V24 to see the relationship between observation parameters. The results showed that the application of a combination of compost and mycorrhizae increased total N content by 78.7%, available P by 26.6%, soil organic C by 29.54%, plant height by 143.87cm, number of leaves by 11.33 leaves, and cob weight by 294.33 g compared to control. The correlation test showed that applying a combination of compost and mycorrhiza affected N in the soil, so the growth and yield of corn were higher.

Influence of Levels of Phosphorus on Yield Components and Yield of Zero Till Maize in Rice-maize (Zea mays L.) Sequence

Aims: Influence of levels of phosphorus on yield components and yield of zero till maize in rice-maize (Zea mays L.) sequence.&#x0D; Methodology: Field experiment was conducted during two successive years at College of Agriculture, Rajendranagar, Hyderabad on rice-zero till maize sequence. The experiment was replicated thrice with 5 levels of P2O5 in rice and 3 levels of P2O5 in maize.&#x0D; Results: The direct application of 60 kg P2O5 ha-1 to maize recorded significantly higher value of yield components, grain and stover yield of maize over lower Phosphorus levels. The residual effect of 100% and /or 75% RDP (recommended dose of Phosphorus) applied to rice had a greater positive influence on the yield components and yield over lower phosphorus levels. The cumulative effect indicated that application of 100% RDP to both the crops and 75 % RDP to rice and 100% RDP to maize had a positive effect on maize and produced significantly greater yield components and yield over other phosphorus treatments.

Effect of Some Fertilizer Combinations of Nitrogen, Phosphorus and Potassium on Some Qualitative Characteristics of Quinoa in Seed

A field experiment was carried out during the season 2020/2021 in Anbar Governorate - Heet district - Sweeb region to study the effect of levels of nitrogen, phosphorus and potassium on some yield characteristics of quinoa plants using three levels of nitrogen (50, 100, 150) kg h−1 and symbolized by (N1, N2, N3) respectively and three levels of phosphorus (22, 44 and 66) kg P ha−1 and took the symbolized (P1, P2, P3) and three levels of potassium (33.2, 66.2, 99.2) kg K.ha−1 The experiment was designed according to the randomized complete block design (RCBD) with three replications. The results of the experiment showed that the highest percentage of protein in quinoa grains reached 16.50% at low fertilizer levels of nitrogen and high levels of phosphorus and potassium when treated N1P3K3, As for the oil percentage, the low levels of nitrogen, phosphorus and potassium gave the highest oil percentage of 4.39%, as well as the phenols gave the highest value of 74.73 mg Gallic gm−1 at medium levels of nitrogen and low levels of potassium and phosphorus 34.20 mg Gallic gm−1 when treated N2P1K1. As for the flavonoids, it gave the highest value of 34.20 mg Rutin gm−1 It was found that there was a strong positive correlation between phenols and flavonoids of r = 0.78. As for saponins it increased with a decrease in the level of nitrogen and an increase in the levels of phosphorus and potassium, as it gave the highest value of 8.21%, that is, when treating N2P1K1, and it was found that there was a strong negative correlation between The percentage of saponins and the percentage of nitrogen in seeds had a value of r=-0.75.

Minerals and Serum Metabolites Profile in Cows Reared on Natural Pastures in a Semi-Arid Area

This study aimed to profile minerals and metabolite levels in cows solely reliant on natural pastures. Mineral plays a significant role in cattle production and reproduction. Measures of serum metabolites are good indicators in disease diagnosis and nutritional status of animals. A total of 179 blood samples were collected from cases of cows of different ages and breeds experiencing dystocia (n=50), downer cow syndrome (n=34), vaginal prolapse (n=16), retained placenta (n=13), and abortions (n=69) following reports submitted to the North-West (NWU) Animal Hospital in Mafikeng Campus. Analysis of minerals and serum metabolite were done using ICP-MSand IDEXX Catalyst Chemistry Analyser. The data were coded and analysed using the Statistical Software for Social Scientists (SPSS) version 25. Descriptive statistics were used to show the distribution of the minerals and serum metabolites levels across the reproductive conditions. The results were significantly different at 5% (P &lt;0.05). Low zinc levels were seen in cows with abortions and dystocia. In downer cow syndrome, the levels of iron, magnesium and phosphorus were high. Low Iodine levels were seen in cows with vaginal prolapse, retained placenta, abortion and dystocia. Low phosphorus levels were noted in cows with vaginal prolapse. Significant differences were seen in the concentrations of Urea/BUN, Total bilirubin, Aspartate amino-transferase (AST), ammonia and lipase. The levels of minerals and serum metabolites are significant indicators in cows presenting with different reproductive conditions and could affect production.

Analysis of clinical presentation and genetic characteristics of malignant infantile osteopetrosis

Objective: To investigate the clinical presentation and genetic characteristics of malignant infantile osteopetrosis. Methods: This was a retrospective case study. Thirty-seven children with malignant infantile osteopetrosis admitted into Beijing Children's Hospital from January 2013 to September 2022 were enrolled in this study. According to the gene mutations, the patients were divided into the CLCN7 group and the TCIRG1 group. Clinical characteristics, laboratory tests, and prognosis were compared between two groups. Wilcoxon test or Fisher exact test were used in inter-group comparison. The survival rate was estimated with the Kaplan-Meier method and the Log-Rank test was used to compare the difference in survival between groups. Results: Among the 37 cases, there were 22 males and 15 females. The age of diagnosis was 0.5 (0.2, 1.0) year. There were 13 patients (35%) and 24 patients (65%) with mutations in CLCN7 and TCIRGI gene respectively. Patients in the CLCN7 group had an older age of diagnosis than those in the TCIRGI group (1.2 (0.4, 3.6) vs. 0.4 (0.2, 0.6) years, Z=-2.60, P=0.008). The levels of serum phosphorus (1.7 (1.3, 1.8) vs. 1.1 (0.8, 1.6) mmol/L, Z=-2.59, P=0.010), creatine kinase isoenzyme (CK-MB) (457 (143, 610) vs. 56 (37, 82) U/L, Z=-3.38, P=0.001) and the level of neutrophils (14.0 (9.9, 18.1) vs. 9.2 (6.7, 11.1) ×109/L, Z=-2.07, P=0.039) at diagnosis were higher in the CLCN7 group than that in the TCIRG1 group. However, the level of D-dimer in the CLCN7 group was lower than that in the TCIRGI group (2.7 (1.0, 3.1) vs. 6.3 (2.5, 9.7) μg/L, Z=2.83, P=0.005). After hematopoietic stem cell transplantation, there was no significant difference in 5-year overall survival rate between the two groups (92.3%±7.4% vs. 83.3%±7.6%, χ²=0.56, P=0.456). Conclusions: TCIRGI gene mutations are more common in children with osteopetrosis. Children with TCIRGI gene mutations have younger age, lower levels of phosphorus, CK-MB, and neutrophils and higher level of D-dimer at the onset. After hematopoietic stem cell transplantation, patients with CLCN7 or TCIRGI gene mutations have similar prognosis.

The Effects of Rhizophagus irregularis Inoculation on Transcriptome of Medicago lupulina Leaves at Early Vegetative and Flowering Stages of Plant Development.

The study is aimed at revealing the effects of Rhizophagus irregularis inoculation on the transcriptome of Medicago lupulina leaves at the early (second leaf formation) and later (flowering) stages of plant development. A pot experiment was conducted under conditions of low phosphorus (P) level in the substrate. M. lupulina plants were characterized by high mycorrhizal growth response and mycorrhization parameters. Library sequencing was performed on the Illumina HiseqXTen platform. Significant changes in the expression of 4863 (padj < 0.01) genes from 34049 functionally annotated genes were shown by Massive Analysis of cDNA Ends (MACE-Seq). GO enrichment analysis using the Kolmogorov-Smirnov test was performed, and 244 functional GO groups were identified, including genes contributing to the development of effective AM symbiosis. The Mercator online tool was used to assign functional classes of differentially expressed genes (DEGs). The early stage was characterized by the presence of six functional classes that included only upregulated GO groups, such as genes of carbohydrate metabolism, cellular respiration, nutrient uptake, photosynthesis, protein biosynthesis, and solute transport. At the later stage (flowering), the number of stimulated GO groups was reduced to photosynthesis and protein biosynthesis. All DEGs of the GO:0016036 group were downregulated because AM plants had higher resistance to phosphate starvation. For the first time, the upregulation of genes encoding thioredoxin in AM plant leaves was shown. It was supposed to reduce ROS level and thus, consequently, enhance the mechanisms of antioxidant protection in M. lupulina plants under conditions of low phosphorus level. Taken together, the obtained results indicate genes that are the most important for the effective symbiosis with M. lupulina and might be engaged in other plant species.

Serendipita indica is a biostimulant that improves tea growth at low P levels by modulating P acquisition and hormone levels

Serendipita indica (S.i.) exhibits mycorrhizal fungus-like characteristics, while it is unclear how S.i. improves root growth of tea seedlings at low phosphorus (P) levels. This study investigated the effects of S.i. on biomass production, auxin and cytokinin levels, root P levels, soil Olsen-P levels, soil phosphatase activities, and root P transporter (PT) gene expression in tea (Camellia sinensis) seedlings at normal (50 mM P) and low (0.5 mM P) P levels. S.i. colonized tea roots. S.i. inoculation significantly increased shoot and root biomass production at low and normal P levels, as well as root indolebutyric acid, isopentenyladenine, and trans-zetin levels at low P levels. Furthermore, S.i. inoculation significantly raised root P and soil Olsen-P levels at low and normal P levels, accompanied with an increase in soil acid and neutral phosphatase activities at low P levels. S.i. inoculation increased root CsPT1 expression at low P levels. In summary, S.i. can be considered as a biostimulant to improve tea growth and root P acquisition at low P levels.

SAT191 Primary Hyperparathyroidism With Low-Normal Parathyroid Hormone Levels: Phosphorus Can Be The Key

Abstract Disclosure: D. He: None. T. Tavdy: None. Y. Tomer: None. D. Kalbi: None. V. Tabatabaie: None. Background: Primary hyperparathyroidism (PHPT) is characterized by the inappropriate secretion of PTH that leads to hypercalcemia. In patients with PHPT, 80-90% have PTH levels above the normal range and 10-20% have inappropriately “normal” PTH levels, most often in the high-normal range1. PTH levels below or in the lower end of the normal range prompt investigation of non-PTH mediated causes of hypercalcemia. We present a case of PHPT with low-normal PTH levels, and discuss how phosphorus levels guided our diagnosis of an underlying parathyroid adenoma. Clinical Case: An 83-year-old woman was found to have hypercalcemia with corrected calcium ∼13 mg/dL (8.5 - 10.5 mg/dL) and PTH of 36.7 pg/mL (20.0 - 80.0 pg/mL). Phosphorus was consistently low, ranging from 2.1 to 2.4 mg/dL (2.5 - 4.5 mg/dL). PTHrP was 18 pg/mL (11-20 pg/mL). 25-hydroxy vitamin D was 24.5 ng/mL (30-60 ng/mL). 1,25-dihydroxy vitamin D was 36 pg/mL (18-72 pg/mL). Serum and urine protein electrophoresis were normal. HTLV-1/2 antibody was negative. TSH was 1.15 uU/mL (0.30 - 4.20 uU/mL). The patient had no history of ingesting lithium or thiazide diuretics. Suspecting PHPT, the patient underwent a nuclear medicine parathyroid scan which showed abnormal parathyroid tissue in the left thyroid lobe. In the setting of volume overload, management of the patient’s persistent hypercalcemia of greater than 12 mg/dL (8.5 - 10.5 mg/dL) began initially with a dose of pamidronate 60 mg and gentle hydration followed by furosemide. Corrected calcium improved to 12.3 mg/dL (8.5 - 10.5 mg/dL). Given the patient’s decreased functional status (bedbound) and high surgical risk, after discussion with the patient and her family, a decision was made to proceed with medical management and not with parathyroidectomy. She was treated with cinacalcet 30 mg twice daily. The goal calcium of &amp;lt; 1 unit over the upper limit of normal (11.5 mg/dL) was achieved on day of discharge of the patient back to her nursing home. Conclusion: In patients with hypercalcemia, low serum phosphorus can point to a PTH- or PTHrP-mediated process. Inappropriate secretion of PTH or PTHrP results in high serum calcium and low phosphorus levels by increasing bone resorption and renal calcium reabsorption, and decreasing renal phosphorus reabsorption. This case shows that in patients with hypercalcemia and concomitant hypophosphatemia with normal PTHrP, PHPT should still be considered as a potential etiology, even in the setting of a low-normal PTH level. In our patient, the presence of a parathyroid adenoma was demonstrated with a conclusive parathyroid scan.

THU466 XLH During Pregnancy: A Closer Look Based On Real-World Experience

Abstract Disclosure: D.S. Ali: None. A.A. Khan: Advisory Board Member; Self; Amgen Inc, Takeda. Grant Recipient; Self; Alexion Pharmaceuticals, Inc., Amgen Inc, Radius Health, Inc, Takeda, Ultragenyx. Background: X-linked hypophosphatemia (XLH) is a genetic disorder caused by mutation in the PHEX gene leading to excess serum levels of the phosphate-regulating hormone fibroblast growth factor 23 (FGF23). High FGF23 causes renal phosphate wasting, decreased production of serum 1,25-dihydroxy vitamin D, and increased metabolism of 1,25-dihydroxy vitamin D, leading to hypophosphatemia. Maintaining normal serum phosphorus levels in pregnant women with XLH is critically important to ensure mineralization of the fetal skeleton. Multiple factors may influence phosphorus homeostasis in pregnancy, including an increase in PTHrP production and a tripling of calcitriol beginning in the first trimester. Case study: 30 years old Gravida 1 Para 0 with XLH, diagnosed at the age of 18 months. She has a pathogenic heterozygous mutation of the PHEX gene (c.1645+1G&amp;gt;A). Treated with phosphate and calcitriol since diagnosis. Had no history of neonatal seizure or respiratory distress, and no dental or renal manifestations. No prior fractures or corrective surgeries and has a normal BMD. No history of enthesopathy, however, has chronic joint pain &amp;gt; 1 year. The pregnancy progressed well with no issues; the patient chose not to undergo amniocentesis for fetal diagnosis. She was monitored closely every 3 weeks, aiming to maintain the serum phosphorus within the normal range to ensure adequate mineralization of the fetal skeleton. The requirements for phosphate increased by 50% in the 2nd trimester and by 125% in the 3rd trimester compared to baseline. While the calcitriol dose increased by 25% in the 3 trimesters compared to baseline. Because further increases in the calcitriol dose were associated with hypercalciuria, the dose was carefully titrated. The mean serum phosphorus level was maintained in the low normal reference range at 0.79 - 0.8 mmol/L (NR: 0.8-1.45), mean TmP/GFR 0.611 (NR: 0.96-1.44). She was induced at 37 weeks due to cholestasis, and delivered with a vaginal delivery. The baby boy weighed 2.690 kg and had slightly low serum phosphorus and calcium levels at birth, which corrected spontaneously. DNA analysis confirmed that the baby also has a pathogenic mutation of the PHEX gene. Conclusion: There was a noticeable variation in the serum phosphorus levels during the 1st and 2nd trimesters and less so in the 3rd trimester and during lactation. The dose of phosphate and active vitamin D required careful upward titration to maintain a normal serum phosphorus level. Therefore, close monitoring is essential. Although the rate of c-sections is higher (76%) amongst the XLH patient population compared to the normal population 27.1%, our patient delivered vaginally with no adverse skeletal outcomes in the mother or neonate. Presentation: Thursday, June 15, 2023

Comparison of microalgae cultivation modes for advanced wastewater purification and pollutant-resource conversion: A pilot study

Microalgae absorb CO2 and nutrients (e.g., nitrogen, phosphorus) for biomass growth, which has high potential for wastewater purification and pollutant-resource conversion. However, current studies mainly focused on short-term effect on stimulated water in lab. In this study, the feasibility of pilot-scale microalgae cultivation in real wastewater with low pollutant content and no sterilization was evaluated, and the optimal culture mode was proposed. It was found that microalgae could remove >95 % of nitrogen and phosphorus during the batch cultivation, proving the strong purifying ability of wastewater with low level of nitrogen and phosphorus. However, the high ratio of nitrogen to phosphorus led to long-term phosphorus shortage in the semi-continuous cultivation mode. The fluctuation of wastewater quality/quantity broke the balance of suspended microalgae growth and wash-out in the continuous cultivation mode. Besides, the aggregation of microalgae at the bottom inhibited the sustainable wastewater purification in both semi-continuous and continuous cultivation modes. Finally, continuous cultivation mode of attached microalgae (CCMA) was proposed with stable and efficient performance of wastewater treatment, and the process of microalgae-water separation could be omitted. Under this mode, the average removal of COD, TN, and TP reached 39.33 %, 21.27 % and 88.10 %, respectively, which could be further enhanced by optimizing the carrier density. Therefore, CCMA was a promising approach for scale-up low-pollutant wastewater treatment.

Soil Chemical Attributes and Foliar Nutrient Contents of Native Species in Rehabilitation Chronosequence After Iron Ore Mining

ABSTRACT The mineland rehabilitation can be a challenge due to high soil density and low levels of organic matter and nutrients. This study assessed soil chemical attributes and leaf nutritional contents of two native species (Mimosa acutistipula var. ferrea and Vismia baccifera) grown in an iron waste pile under environmental rehabilitation in the Carajás Mineral Province, Eastern Amazon, Brazil. Soil samples were collected close to plant species in three different chronosequences of rehabilitation. Regardless of the rehabilitation stage, the soils have low levels of phosphorus, potassium, calcium, magnesium, and boron. A tendency of organic matter accumulation was observed at the advanced stage (5–8 years after the onset of rehabilitation process) and it was correlated with other soil nutrients. The leaf nutrient’ contents of M. acutistipula and V. baccifera tend to be distinct from each other, which could indicate that the studied species have different nutritional requirements. The V. baccifera presented higher leaf concentrations of sulfur, copper, and zinc, while M. acutistipula stood out for presenting a higher leaf nitrogen content, which is possibly related to its nitrogen-fixing capacity. In general, an improvement of soil chemical attributes was achieved with the advance of the rehabilitation stages, showing the efficiency of the procedures performed to rehabilitate the studied areas.

Influence of Nitrogen and Plant growth regulators on Growth and Yield of Zaid finger millet (Eleusine coracana L.)

A field experiment was conducted during Zaid season of 2022 at the Crop Research Farm, Department of Agronomy, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (UP) India. To study the Response of Nitrogen and plant growth regulators on growth and yield of zaid finger millet. The treatments consist of Nitrogen 40,60,80 kg/ha and plant growth regulators Salicylic acid 40ppm, Benzyl Amino Purine 50ppm, Chlormequat chloride 200 ppm. There were 10 treatments each replicated thrice. During the experiment, the soil in the experimental plot had a sandy loam texture, nearly neutral in soil reaction (pH 7.8), had a low level of organic carbon (0.35%) and had a low level of available Nitrogen (163.42 kg/ha), available Phosphorus (21.96 kg/ha) and available Potassium (256.48 kg/ha). Results revealed that the higher plant height (79.76 cm), number of tillers/plant (7.85/plant), plant dry weight (25.92g/plant), seed yield (3.25 t/ha), straw yield (4.81 t/ha) and Harvest index (40.34%) were significantly influenced with application of nitrogen 80 kg/ha + salicylic acid 40 ppm.