This study investigates the variations of middle and low latitude sporadic-E (Es) layers in response to a geomagnetic storm. Es layers are observed by five ionosondes located in the Eastern Asian sector. The critical frequencies of Es layers (foEs) at six stations increased in sequence from high latitude stations to low latitude stations after IMF/Bz turning southward. Lomb–Scargle analysis shows the amplification of semidiurnal oscillation amplitude in the vertical height of Es layers during geomagnetic disturbance. Modeling results of the NCAR Thermosphere-Ionosphere-Electrodynamics General Circulation Model (TIEGCM) show the enhancement of the wind field in the mesosphere and the lower thermosphere (MLT) region. Our study provides evidence that the enhanced wind field in the MLT region during the storm period could result in the enhancement of Es layers at middle and low latitude.