Low-intensity Vibration Research Articles

Parameter-Dependency of Low-Intensity Vibration for Wound Healing in Diabetic Mice.

Chronic wounds in diabetic patients represent an escalating health problem, leading to significant morbidity and mortality. Our group previously reported that whole body low-intensity vibration (LIV) can improve angiogenesis and wound healing in diabetic mice. The purpose of the current study was to determine whether effects of LIV on wound healing are frequency and/or amplitude dependent. Wound healing was assessed in diabetic (db/db) mice exposed to one of four LIV protocols with different combinations of two acceleration magnitudes (0.3 and 0.6 g) and two frequencies (45 and 90 Hz) or in non-vibration sham controls. The low acceleration, low frequency protocol (0.3 g and 45 Hz) was the only one that improved wound healing, increasing angiogenesis and granulation tissue formation, leading to accelerated re-epithelialization and wound closure. Other protocols had little to no impact on healing with some evidence that 0.6 g accelerations negatively affected wound closure. The 0.3 g, 45 Hz protocol also increased levels of insulin-like growth factor-1 and tended to increase levels of vascular endothelial growth factor in wounds, but had no effect on levels of basic fibroblast growth factor or platelet derived growth factor-bb, indicating that this LIV protocol induces specific growth factors during wound healing. Our findings demonstrate parameter-dependent effects of LIV for improving wound healing that can be exploited for future mechanistic and therapeutic studies.

Вплив механічних коливань на структурну організацію великогомілкової кістки у щурів з глюкокортикоїдіндукованим остеопорозом

The aim of the study was to evaluate the effect of nonphysiological whole body vibration (0,3g) on the bone structure and metabolism in rats treated with methylprednisolone (3 mg/ kg/day every other day, 24 weeks). Amount of crystalline component and collagen in the bones was determined by X-ray diffraction method, and the level of calcium by atomic adsorption spectroscopy. Bone metabolism was assessed by determining the concentration of markers - osteocalcinandtartrate-resistant acid phosphatase 5b. Methylprednisolone reduced the content of the mineral component in the tibia (–16.8%) in I group compared with the control. This significantly accelerated the process of bone metabolism, as evidenced by the increased level of bone remodeling markers. It should be noted that the total nonphysiological whole body vibration did not allow a decrease in the mineral component of the bone until 16 weeks of the experiment compared with I group, although these values were lower than the control group (–28.3%). We suggests that mechanical high-frequency low-intensity whole body vibration can inhibit the negative effects of glucocorticoids on bone structure.

Repair and remodeling of partial-weightbearing, uninstrumented long bone fracture model in mice treated with low intensity vibration therapy

BackgroundWhile vibration therapy has shown encouraging results across many fields of medicine in the last decade, its role as originally envisioned for bone health remains uncertain. Especially regarding its efficacy in promoting fracture healing, mixed and incomplete outcomes suggest a need to clarify its potential. In particular, the definitive effect of vibration, when isolated from the confounding mechanical inputs of gait and stabilizing instrumentation, remains largely unknown. MethodsFour cohorts of C57BL/6 male mice underwent single-leg, open fibula fracture. Vibration was applied at 0.3 g to two groups for 20 min/d. At 3 and 6 weeks, fibulae were harvested for microcomputed tomography and 3-point bending to failure. FindingsIn bone volume and tissue volume, the groups at each healing time point were statistically not different. At 3 weeks, however, the ratio of bone-to-tissue volume was lower for the vibrated group than control. Likewise, while bone mineral density did not differ, tissue volume density was lowest with vibration. At 6 weeks, mean differences were nominal. Biomechanically, vibration consistently trended ahead of control in strength and stiffness, but did not achieve statistical significance. InterpretationAt this stage of therapeutic development, vibration therapy in isolation does not demonstrate a clear efficacy for bone healing, although further treatment permutations and translational uses remain open for investigation.

Low-intensity vibration restores nuclear YAP levels and acute YAP nuclear shuttling in mesenchymal stem cells subjected to simulated microgravity

Reducing the musculoskeletal deterioration that astronauts experience in microgravity requires countermeasures that can improve the effectiveness of otherwise rigorous and time-expensive exercise regimens in space. The ability of low-intensity vibrations (LIV) to activate force-responsive signaling pathways in cells suggests LIV as a potential countermeasure to improve cell responsiveness to subsequent mechanical challenge. Mechanoresponse of mesenchymal stem cells (MSC), which maintain bone-making osteoblasts, is in part controlled by the “mechanotransducer” protein YAP (Yes-associated protein), which is shuttled into the nucleus in response to cyto-mechanical forces. Here, using YAP nuclear shuttling as a measurement outcome, we tested the effect of 72 h of clinostat-induced simulated microgravity (SMG) and daily LIV application (LIVDT) on the YAP nuclear entry driven by either acute LIV (LIVAT) or Lysophosphohaditic acid (LPA), applied after the 72 h period. We hypothesized that SMG-induced impairment of acute YAP nuclear entry would be alleviated by the daily application of LIVDT. Results showed that while both acute LIVAT and LPA treatments increased nuclear YAP entry by 50 and 87% over the basal levels in SMG-treated MSCs, nuclear YAP levels of all SMG groups were significantly lower than non-SMG controls. LIVDT, applied in parallel to SMG, restored the SMG-driven decrease in basal nuclear YAP to control levels as well as increased the LPA-induced but not LIVAT-induced YAP nuclear entry over SMG only, counterparts. These cell-level observations suggest that daily LIV treatments are a feasible countermeasure for restoring basal nuclear YAP levels and increasing the YAP nuclear shuttling in MSCs under SMG.

Mechanical suppression of breast cancer cell invasion and paracrine signaling to osteoclasts requires nucleo-cytoskeletal connectivity

Exercise benefits the musculoskeletal system and reduces the effects of cancer. The effects of exercise are multifactorial, where metabolic changes and tissue adaptation influence outcomes. Mechanical signals, a principal component of exercise, are anabolic to the musculoskeletal system and restrict cancer progression. We examined the mechanisms through which cancer cells sense and respond to low-magnitude mechanical signals introduced in the form of vibration. Low-magnitude, high-frequency vibration was applied to human breast cancer cells in the form of low-intensity vibration (LIV). LIV decreased matrix invasion and impaired secretion of osteolytic factors PTHLH, IL-11, and RANKL. Furthermore, paracrine signals from mechanically stimulated cancer cells, reduced osteoclast differentiation and resorptive capacity. Disconnecting the nucleus by knockdown of SUN1 and SUN2 impaired LIV-mediated suppression of invasion and osteolytic factor secretion. LIV increased cell stiffness; an effect dependent on the LINC complex. These data show that mechanical vibration reduces the metastatic potential of human breast cancer cells, where the nucleus serves as a mechanosensory apparatus to alter cell structure and intercellular signaling.

An ultralow frequency, low intensity, and multidirectional piezoelectric vibration energy harvester using liquid as energy-capturing medium

Herein, a piezoelectric vibration energy harvester (PVEH) using liquid as an energy-capturing medium is proposed to simultaneously achieve ultralow frequency, low intensity, and multidirectional vibration energy harvesting in a horizontal plane, which is difficult to realize using traditional PVEHs. The proposed harvester comprises a cylindrical container with a certain liquid, a piezoelectric cantilever beam, ropes, and floater-lever arrays. The experimental results indicate that the proposed harvester with a single floater-lever can generate 9.8 μW under an ultralow frequency (2.6 Hz) and a low intensity vibration excitation (0.03 g), and the normalized power density is 8.89 μW/(cm3 g2 Hz). Under a multidirectional vibration excitation (360° in the horizontal plane) with frequencies below 3 Hz and an acceleration of 0.03 g, the two proposed harvesters with three and four floater-levers indicate a maximum output power (Pmax) deviation of 24.92% and 28.31%, respectively, and an angle bandwidth of 360° (using 2/2Pmax as the standard). All the experimental results indicate that the proposed PVEH is highly promising as an energy supply of wireless sensor networks distributed in ultralow frequency, low intensity, and multidirectional applications.

Isolated nuclei stiffen in response to low intensity vibration

The nucleus, central to all cellular activity, relies on both direct mechanical input and its molecular transducers to sense and respond to external stimuli. While it has been shown that isolated nuclei can adapt to applied force ex vivo, the mechanisms governing nuclear mechanoadaptation in response to physiologic forces in vivo remain unclear. To investigate nuclear mechanoadaptation in cells, we developed an atomic force microscopy (AFM) based procedure to probe live nuclei isolated from mesenchymal stem cells (MSCs) following the application of low intensity vibration (LIV) to determine whether nuclear stiffness increases as a result of LIV. Results indicated that isolated nuclei were, on average, 30% softer than nuclei tested within intact MSCs prior to LIV. When the nucleus was isolated following LIV (0.7 g, 90 Hz, 20 min) applied four times (4×) separated by 1 h intervals, stiffness of isolated nuclei increased 75% compared to non-LIV controls. LIV-induced nuclear stiffening required functional Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, but was not accompanied by increased levels of the nuclear envelope proteins LaminA/C or Sun-2. While depleting LaminA/C or Sun-1&2 resulted in either a 47% or 39% increased heterochromatin to nuclear area ratio in isolated nuclei, the heterochromatin to nuclear area ratio was decreased by 25% in LIV-treated nuclei compared to controls, indicating LIV-induced changes in the heterochromatin structure. Overall, our findings indicate that increased apparent cell stiffness in response to exogenous mechanical challenge of MSCs in the form of LIV is in part retained by increased nuclear stiffness and changes in heterochromatin structure.

Applicability of Low-intensity Vibrations as a Regulatory Factor on Stem and Progenitor Cell Populations.

Persistent and transient mechanical loads can act as biological signals on all levels of an organism. It is therefore not surprising that most cell types can sense and respond to mechanical loads, similar to their interaction with biochemical and electrical signals. The presence or absence of mechanical forces can be an important determinant of form, function and health of many tissue types. Along with naturally occurring mechanical loads, it is possible to manipulate and apply external physical loads on tissues in biomedical sciences, either for prevention or treatment of catabolism related to many factors, including aging, paralysis, sedentary lifestyles and spaceflight. Mechanical loads consist of many components in their applied signal form such as magnitude, frequency, duration and intervals. Even though high magnitude mechanical loads with low frequencies (e.g. running or weight lifting) induce anabolism in musculoskeletal tissues, their applicability as anabolic agents is limited because of the required compliance and physical health of the target population. On the other hand, it is possible to use low magnitude and high frequency (e.g. in a vibratory form) mechanical loads for anabolism as well. Cells, including stem cells of the musculoskeletal tissue, are sensitive to high frequency, lowintensity mechanical signals. This sensitivity can be utilized not only for the targeted treatment of tissues, but also for stem cell expansion, differentiation and biomaterial interaction in tissue engineering applications. In this review, we reported recent advances in the application of low-intensity vibrations on stem and progenitor cell populations. Modulation of cellular behavior with low-intensity vibrations as an alternative or complementary factor to biochemical and scaffold induced signals may represent an increase of capabilities in studies related to tissue engineering.

Low Intensity Vibrations Augment Mesenchymal Stem Cell Proliferation and Differentiation Capacity during in vitro Expansion

A primary component of exercise, mechanical signals, when applied in the form of low intensity vibration (LIV), increases mesenchymal stem cell (MSC) osteogenesis and proliferation. While it is generally accepted that exercise effectively combats the deleterious effects of aging in the musculoskeletal system, how long-term exercise affects stem cell aging, which is typified by reduced proliferative and differentiative capacity, is not well explored. As a first step in understanding the effect of long-term application of mechanical signals on stem cell function, we investigated the effect of LIV during in vitro expansion of MSCs. Primary MSCs were subjected to either a control or to a twice-daily LIV regimen for up to sixty cell passages (P60) under in vitro cell expansion conditions. LIV effects were assessed at both early passage (EP) and late passage (LP). At the end of the experiment, P60 cultures exposed to LIV maintained a 28% increase of cell doubling and a 39% reduction in senescence-associated β-galactosidase activity (p < 0.01) but no changes in telomere lengths and p16INK4a levels were observed. Prolonged culture-associated decreases in osteogenic and adipogenic capacity were partially protected by LIV in both EP and LP groups (p < 0.05). Mass spectroscopy of late passage MSC indicated a synergistic decrease of actin and microtubule cytoskeleton-associated proteins in both control and LIV groups while LIV induced a recovery of proteins associated with oxidative reductase activity. In summary, our findings show that the application of long-term mechanical challenge (+LIV) during in vitro expansion of MSCs for sixty passages significantly alters MSC proliferation, differentiation and structure. This suggests LIV as a potential tool to investigate the role of physical activity during aging.

Local low‐intensity vibration improves healing of muscle injury in mice

Recovery from traumatic muscle injuries is typically prolonged and incomplete. Our previous study demonstrated that whole‐body low‐intensity vibration (LIV) enhances healing in a mouse laceration model. We sought to determine whether locally applied LIV (a) improves muscle repair following injury in mice and (b) is directly transduced by cultured muscle cells, via increased IGF‐1 activity. C57BL/6J mice were subjected to laceration of the gastrocnemius muscle and were treated with LIV applied directly to the lower leg for 30 min/day or non‐LIV sham treatment (controls) for 7 or 14 days. LIV was also applied to differentiating myotubes in culture for 30 min/day for 3 or 6 days. Compared with control mice, LIV increased myofiber cross‐sectional area, diameter, and percent area of peripherally nucleated fibers, and decreased percent damaged area after 14 days of treatment. In cultured myotubes, LIV increased fusion and diameter compared with controls after 6 days of treatment. These LIV‐induced effects were associated with increased total Akt on day 7 in injured muscle and on day 3 in myotubes, whereas phosphorylated‐to‐total Akt ratio increased on day 14 in injured muscle and on day 6 in myotubes but were not associated with increased IGF‐1 levels at any time point. These changes were also associated with LIV‐induced suppression of FOXO1 and Atrogin‐1 gene expression at day 7 in injured muscle. These findings demonstrate that muscle cells can directly transduce LIV signals into increased growth and differentiation, and this effect is associated with increased Akt signaling.

Low-intensity vibration impact on a low-permeable coal seam

With a sufficiently high level of degassing works in the mines of a number of coal basins, their effectiveness is constrained by specific properties of the coal seams being mined. In particular, the filtration and reservoir properties of fossil coal differ significantly, in natural conditions, even within one coal seam. The article outlines the purpose of the new method, namely, the vibration impact on the coal seam to increase fracturing and as a consequence of increasing the gas recovery intensity. In this way, when mining a low-permeable gas-saturated coal seam, the gas recovery rate is very low due to the physical state of the array. Existing methods create insufficient conditions for the degassing of coal seams. Therefore, to increase the efficiency of the coal array it is required to create new, more efficient ways to crack open fractures and increase gas recovery from coal. This is achieved by applying vibration to a low-permeable gas-bearing coal seam.

Effects of Whole Body Vibration Training on Inflammatory Markers in Young Healthy Males

Background: The intensity of the vibration load refers to frequency and amplitude. Objectives: The present study aimed at reviewing the effects of high and low intensity whole body vibration training on inflammatory markers. Methods: Overall, 30 healthy male patients were divided to three groups, including control (N = 8), high intensity (N = 11), and low intensity (N = 11) groups. Training included consecutive weeks, three times each week with low intensity (amplitudes of 2 mm), high intensity (amplitudes of 4 mm), and progressive frequencies. Blood samples were analyzed for concentrations of high sensitivity C-reactive protein (hsCRP) and fibrinogen before and after completion of training. Paired sample t-test and One-way Analysis of Variance (ANOVA) were used to analyze the research findings (P ≤ 0.05). Results: Whole body vibration training with low and high intensities had no significant effects on hsCRP and fibrinogen levels (P ≥ 0.05). Conclusions: It appears that six weeks of low and high intensity vibration training has the same effects on CRP and fibrinogen.

Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex

Mesenchymal stem cells (MSC) rely on their ability to integrate physical and spatial signals at load bearing sites to replace and renew musculoskeletal tissues. Designed to mimic unloading experienced during spaceflight, preclinical unloading and simulated microgravity models show that alteration of gravitational loading limits proliferative activity of stem cells. Emerging evidence indicates that this loss of proliferation may be linked to loss of cellular cytoskeleton and contractility. Low intensity vibration (LIV) is an exercise mimetic that promotes proliferation and differentiation of MSCs by enhancing cell structure. Here, we asked whether application of LIV could restore the reduced proliferative capacity seen in MSCs that are subjected to simulated microgravity. We found that simulated microgravity (sMG) decreased cell proliferation and simultaneously compromised cell structure. These changes included increased nuclear height, disorganized apical F-actin structure, reduced expression, and protein levels of nuclear lamina elements LaminA/C LaminB1 as well as linker of nucleoskeleton and cytoskeleton (LINC) complex elements Sun-2 and Nesprin-2. Application of LIV restored cell proliferation and nuclear proteins LaminA/C and Sun-2. An intact LINC function was required for LIV effect; disabling LINC functionality via co-depletion of Sun-1, and Sun-2 prevented rescue of cell proliferation by LIV. Our findings show that sMG alters nuclear structure and leads to decreased cell proliferation, but does not diminish LINC complex mediated mechanosensitivity, suggesting LIV as a potential candidate to combat sMG-induced proliferation loss.

Influence of Hydrodynamic Processes Generated by 1.94-μm Pulsed Laser Radiation on Daphnia magna Crustaceans

The hydrodynamic processes occurring in water under the action of pulsed laser radiation with a power of 20 W, wavelength of 1.94 μm, and a pulse duration of 100 ns were investigated. Such radiation leads the excitation of jet flows in water, as well as microbubbles and broadband acoustic vibrations. It was established that the main energy of these vibrations lies within the range of 10–15 kHz; they are excited according to the thermal cavitation mechanism and the regime corresponds to superintense nucleate boiling. It was shown that laser-induced hydrodynamic processes exert a pronounced biological effect on Daphnia magna crustaceans, leading to their increased fertility for acoustic doses of 35 and 350 J/m2. The experimental data and theoretical estimates show that stimulation of the reproductive function in crustaceans is due to the action of laser-induced low-intensity broadband acoustic vibrations and is not associated with temperature effects. The death of crustaceans and appearance of individuals with maldevelopments were observed for the maximum exposure (300 s). It is shown that the negative effects are related only to the effect of high-temperature microjets on Daphnias.

Реакция модели здания со снижением жесткости на длиннопериодные сейсмические воздействия

Long-period earthquakes with dominant periods of about 2...5 s occur in the far zone and usually have low intensity. However, such earthquakes can cause devastating consequences for structures if, first, their vibration frequencies are close to the lowest natural frequencies of the structures, and, second, if they persist for a sufficiently long time. The aim of this work is to simulate a situation in which low-intensity seismic vibration acts on a structure for a long time with close-to-resonant frequencies. It leads to a long-term oscillatory process with large amplitudes. Such conditions can give rise to cyclic elastic-plastic deformation of the system with accumulation of damages, with degradation of stiffness and with decreasing of natural frequencies. If during a long-period earthquake the prevailing seismic load frequencies also decrease, the structure is “self-tuned” to resonance, a circumstance that aggravates the seismic load consequences. The dynamic response of a system with time-dependent decreasing of stiffness for long-period seismic oscillations was analyzed for the first time. The Tohoku earthquake accelerogram recorded in Tokyo on March 11, 2011 at the TKY017 station was taken as the seismic load. The prevailing seismic load frequencies in the selected record decrease from 0.83 Hz to 0.3 Hz. Displacements and their standard deviations for systems with the initial natural frequencies equal to 0.83 Hz, 1 Hz, and 0.5 Hz were obtained and analyzed. All calculations, including preprocessing of seismic load data, implementation of the hysteresis algorithm with decrease of stiffness, integration of the motion equations, and analysis of the obtained results were all accomplished in the Matlab software system environment.

Effects of low-intensity vibration with rest period insertion on osteoporotic bone structure and the involvement of bone microvasculature

Effects of low-intensity vibration with rest period insertion on osteoporotic bone structure and the involvement of bone microvasculature

Quantitative ultrasound imaging monitoring progressive disuse osteopenia and mechanical stimulation mitigation in calcaneus region through a 90-day bed rest human study

BackgroundOsteoporosis parallels aging and functional mechanical unloading (e.g., space flight and bed rest), jeopardizing mineral density, microstructure, and integrity of bone and leading to an increased risk of fracture. A way to combat this deterioration is to harness the sensitivity of bone to mechanical signals. ObjectiveThis study evaluates the longitudinal effect of a dynamic mechanical loading through the heel on human bone in vivo during 90-day bed rest, monitored by quantitative ultrasound (QUS) imaging and dual-energy X-ray absorptiometry (DXA) in localized regions of interests, i.e., calcaneus. MethodsA total of 29 bed rest individuals were evaluated (11 control and 18 treatment) with a brief (10-minute) daily low-intensity (0.3g), high-frequency (30Hz) dynamic mechanical stimulation countermeasure through vibrational inhibition bone erosion (VIBE). Both QUS and DXA detected longitudinal bone density and quality changes. ResultsUltrasound velocity (UV) decreased in the control group and increased in the group treated with low-intensity loading. The UV increased by 1.9% and 1.6% at 60- and 90-day bed rest (p=0.01) in VIBE over control groups. A trend was found in broadband ultrasound attenuation (BUA), with a VIBE benefit of 1.8% at day 60 and 0.5% at day 90 in comparison with control (p=0.5). Bone mineral density (BMD) assessed by DXA decreased -4.50% for control individuals and -2.18% for VIBE individuals, showing a moderate effect of the mechanical intervention (p=0.19). Significant correlations between QUS and DXA were observed, with a combined BUA and UV vs. BMD: r2=0.70. ConclusionThese results indicated that low-intensity, high-frequency loading has the potential to mitigate regional bone loss induced by long-term bed rest and that QUS imaging may be able to assess the subtle changes in bone alteration. Translational potential of this articleQuantitative ultrasound has shown the efficacy of noninvasively assessing bone mass and structural properties in cadaver and isolated trabecular bone samples. While its ability in measuring in vivo bone quality and density is still unclear, a scanning confocal ultrasound imaging is developed and can perform an instant assessment for the subtle changes of such bone loss. This ultrasound imaging modality can potentially be used in the clinical assessment of bone mass. Moreover, physical stimulation has shown the ability to prevent bone loss induced by functional disuse and estrogen deficiency in animal models. However, its treatment capability is unclear. This study has shown that low-magnitude mechanical signals, introduced using low-intensity vibration (LIV), can mitigate regional bone loss caused by functional disuse. Thus localized mechanical treatment, and the quantitative ultrasound imaging have shown translational potential to noninvasively attenuate bone loss, and assess bone mass in the clinic, e.g., in an extreme condition such as long-term space mission, and long-term bedrest such as in case of spinal cord injury.

A nonlinear multi-stable piezomagnetoelastic harvester array for low-intensity, low-frequency, and broadband vibrations

Abstract A compact nonlinear multi-stable piezomagnetoelastic energy harvester array (MPEHA) is presented in this paper to scavenge energy from low-amplitude (

Increased skin blood flow during low intensity vibration in human participants: Analysis of control mechanisms using short-time Fourier transform.

AimInvestigate the immediate effect of low intensity vibration on skin blood flow and its underlying control mechanisms in healthy human participants.Materials and methodsOne-group pre-post design in a university laboratory setting. Nine adults underwent two bouts of 10-minute vibration (30Hz, peak acceleration 0.4g). Outcome measures include skin blood flow, and skin temperature on the right foot. To examine the control mechanisms underlying the vibration-induced blood flow response, SHORT-TIME Fourier analyses were computed to obtain the spectral densities for three frequency bands: metabolic (0.0095–0.02Hz), neurogenic (0.02–0.06Hz), and myogenic (0.06–0.15Hz). Non-parametric Friedman’s tests were computed to compare changes of the outcome measures and control mechanisms over the course of vibration.ResultsVibration increased skin blood flow during both bouts of vibration, however the effect did not last after vibration was terminated. Myogenic spectral density increased during both bouts of vibration, whereas the metabolic and neurogenic spectral densities increased only during the 2nd bout of vibration. Interestingly, only the metabolic spectral density remained elevated after vibration ended.ConclusionLow intensity vibration produced acute increases in skin blood flow mediated in part by vascular control mechanisms of myogenic origin. Further investigation is warranted to determine whether low intensity vibration induces similar increases in skin blood flow in populations prone to developing chronic non-healing wounds, such as spinal cord injury and diabetes.

Vibration effect on a stability of stationary flow of pseudoplastic fluid in vertical slot

In this paper, we study the effect of vibrations on the stability of a stationary plane-parallel flow of a pseudoplastic fluid in an infinite vertical layer between two rigid perfectly heat conducting boundaries, maintained at different temperatures. It is assumed that the layer performs vertical high-frequency vibrations perpendicular to the imposed temperature gradient. Vibration intensity is characterized by the dimensionless parameter V, typical values of which in the terrestrial conditions vary from 0 to 1. The fluidity of liquid is found to increase with increasing vibration intensity. The stability of a stationary plane-parallel flow with respect to small plane normal-mode perturbations, periodic along the layer, is investigated for Prandtl number values Pr = 1 and Pr = 100. The results show that, for Pr = 1, at any values of rheological parameters under examination, the monotonic hydrodynamic perturbations having a form of immovable vortices at the boundary of counter-current flows are responsible for the instability of a stationary flow. The value of the modified Grashof number, which determines the base flow stability threshold, decreases monotonically as the pseudoplastic properties of fluid become stronger. Vibrations also produce a destabilizing effect. For Pr = 100 there are three instability modes: the monotonic hydrodynamic instability mode and the thermal oscillatory Rayleigh mode, which exist in the absence of vibrations, and the vibrational oscillatory mode. In the case of low-intensity vibrations, the thermal Rayleigh mode is the most dangerous one. An increase in the vibration intensity, vibration mode becomes the most dangerous. The growth of dynamic yield stress leads to a decrease in the value of the vibration parameter V at which the vibrational mode becomes the most dangerous mode.