Low-intensity Laser Radiation Research Articles

NIR-Actuated Botanicals/Nanozymes Nanofibrous Membranes for Antibiotic-Free Triple-Synergistic Therapy Against Polymicrobial Wound Infections.

Polymicrobial wound infection represents a significant contributor to infectious disease-related morbidity and mortality globally. Traditionally, the management of such infection relies heavily on high doses of antibiotics, often resulting in systemic side effects and exacerbating antibiotic resistance. Consequently, there is an urgent need to develop a safe and effective platform for antibiotic-free treatment of polymicrobial wound infections. In this study, a near-infrared (NIR) light-actuated nanofibrous membrane incorporating botanicals and nanozymes is presented for antibiotic-free triple-synergistic therapy against polymicrobial wound infections. The membrane integrates berberine hydrochloride (BBH)-loaded hollow zinc-doped carbon nanocubes into polyvinyl alcohol (PVA) nanofibrous membranes. Upon low-intensity 808nm NIR laser irradiation (0.3Wcm-2), the membrane achieved a controlled release of BBH and enhanced peroxidase-like (POD-like) enzyme activity. This NIR light-actuated botanical/photothermal therapy (PTT)/chemodynamic therapy (CDT) system demonstrated high efficacy in eradicating methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa both in vitro and in vivo. Furthermore, it mitigated inflammation and promoted wound healing in polymicrobial infection wounds, highlighting its potential as a promising alternative to antibiotic-based therapies.

The impact of 980nm diode laser irradiation on the proliferation of mesenchymal stem cells derived from the umbilical cord's

Various cell types can have their growth accelerated by using low-intensity laser radiation. The study is intended to look at the impacts of laser radiation at low energy intensity on the ability of mesenchymal stem cells (MSCs) generated from umbilical cords to proliferate as well as survive in low-nutrient conditions. The study applied two different energy densities, 2.5 j/cm2 and 5 j/cm2, using a 980 nm diode laser radiation. This allowed for the observation of the effects of these specific elements on the behavior of the cells in a controlled environment at various concentrations of fetal bovine (7.5 %, 10 %, 12.5 %, and 15 %). The cells were grown in a medium lacking in nutrients and were enriched with varying quantities of serum from fetal bovines. The MTT test was used to evaluate the mitochondrial activity of the cell. Following 72 hours, it was shown that cells treated with 2.5 j/cm2 and 10 % fetal bovine serum had significantly higher MTT test activity than cells treated with 5 j/cm2.The results of this study show that even in the presence of dietary deficiencies, low-intensity laser radiation therapy can stimulate the growth of mesenchymal stem cells isolated from umbilical cords.

High-frequency low-intensity semiconductor laser irradiation enhances osteogenic differentiation of human cementoblast lineage cells

PurposeLaser irradiation activates a range of cellular processes in the periodontal components and promotes tissue repair. However, its effect on osteogenic differentiation of human cementoblast lineage cells remains unclear. This study aimed to examine the effects of high-frequency semiconductor laser irradiation on the osteogenic differentiation of human cementoblast lineage (HCEM) cells.MethodsHCEM cells were cultured to reach 80% confluence and irradiated with a gallium-aluminum-arsenide (Ga-Al-As) semiconductor laser with a pulse width of 200 ns and wavelength of 910 at a dose of 0–2.0 J/cm2. The outcomes were assessed by analyzing the mRNA levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and type I collagen (COLL1) using real-time polymerase chain reaction (PCR) analysis 24 h after laser irradiation. Cell mineralization was evaluated using ALP activity, calcium deposition, and Alizarin Red staining.ResultsThe laser-irradiated HCEM cells showed significantly enhanced gene expression levels of ALP, RUNX2, and COLL1 as well as ALP activity and calcium concentration in the culture medium compared with the non-irradiated cells. In addition, enhanced calcification deposits were confirmed in the laser-irradiated group compared with the non-irradiated group at 21 and 28 days after the induction of osteogenic differentiation.ConclusionHigh-frequency semiconductor laser irradiation enhances the osteogenic differentiation potential of cultured HCEM cells, underscoring its potential utility for periodontal tissue regeneration.

PHOTOREGULATION OF THE BIOSYNTHETIC ACTIVITY OF LARICIFOMES OFFICINALIS USING COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION

The aim of the work was to study the influence of biogenic metal nanoparticles on the growth characteristics and biosynthetic activity of the fungus Laricifomes officinalis, as well as the effects of photocatalytic activity of NPs after exposure to low-intensity laser radiation under deep cultivation conditions. Material and Methods. Traditional mycological methods, colloidal solutions of nanoparticles biogenic metals, and unique photobiological methods were used. Results. Treatment of the inoculum with colloidal solutions of nanoparticles of all used metals ncreased the growth of L. officinalis by 31–54%, while irradiation of the fungal inoculum with laser light in a medium with nanoparticles reduced the growth activity of the L. officinalis mycelium by 14.4–22.6%. All nanoparticles suppressed the biosynthesis of extracellular polysaccharides, whereas treatment of the inoculum with colloidal solutions of FeNPs and MgNPs stimulated the synthesis of endopolysaccharides. At the same time, laser light irradiation in the presence of AgNPs increased the amount of endopolysaccharides, while FeNPs and MgNPs slightly inhibited their synthesis. Treatment of the inoculum with colloidal metal solutions and laser light affected the total phenolic content (TPC) in the mycelial mass. The highest TPC values in ethanol extracts with AgNPs and laser light irradiation were 97.31Ѓ}3.7 mg of GAEs/g of dry mass. Conclusions. The research results gave ground to consider nanoparticles of biogenic metals (AgNPs, FeNPs, MgNPs) and low-intensity laser light as a promising regulators of the biosynthetic activity of L. officinalis in the biotechnology of its cultivation.

Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.

Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.

Assessment of the efficacy of comprehensive medical rehabilitation of patients with polypoid rhinosinusitis based on the international classification of functioning, disability and health

Abstract. Purpose of the study. The article discusses an algorithm for using the categories of the International Classification of Functioning, Disability and Health (ICF) for comparative assessment of the effectiveness of various medical rehabilitation programs for patients with polypoid rhinosinusitis. It is shown that the use of the ICF makes it possible to implement a systematic approach to assessing the efficacy of rehabilitation measures, taking into account the biopsychosocial model for assessing health (illness) and the category of functioning, which serves as the basis for the integration process of rehabilitation. The results of comparative monitoring of the formed categorical profile of a patient with polypoid rhinosinusitis convincingly proved the advantages of complex medical rehabilitation of patients with nasal polyposis, based on biological therapy with dupilumab in combination with a course application of an alternating magnetic field and low-intensity laser radiation.

The effect of low-intensity laser radiation on the sensitivity of Staphylococcus aureus to some halogen-containing azaheterocycles

The rapid rate of acquisition and spread of resistance to antibiotics by microorganisms leads to the search and investigation of new ways of combating infectious agents. Being a modern, minimally invasive method with an almost complete absence of side effects, the use of low-intensity laser radiation can be considered as one of the alternatives to traditional antibiotic therapy. At the same time, the search for new chemical compounds with pronounced antimicrobial activity is also promising. The impact of low-intensity laser of the red spectrum (λ = 660 nm) on the sensitivity of a clinical isolate of Staphylococcus aureus to newly synthesized halogen- and chalcogen-halogen-containing derivatives of the quinazoline and benzothiazole series was studied using the serial microdilutions method with the determination of minimal inhibitory and minimal bactericidal concentrations. To evaluate the antimicrobial properties of the investigated substances in dynamic, we investigated their activity two months after the synthesis with subsequent comparison to freshly synthesized compounds. The research results indicate that the most pronounced antimicrobial effect was shown by trichlorotelluromethylthiazoloquinazolinium chloride and propargylthiobenzothiazolium hexachlorotellurate. Evaluating the dynamics of the activity of the studied compounds, it was noted that the absolute majority of substances retained their properties, which indicates their stability. When evaluating the effect of irradiation of microbial inoculum with low-intensity laser radiation on the susceptibility to chemical compounds, an increase in the sensitivity of irradiated microorganisms to some investigated chemicals was noted compared to similar non-irradiated microbial suspensions. In the case of 2 out of 9 studied chemical compounds we noted a 2- to 4-fold decrease in the minimal inhibitory concentration for irradiated microbial suspensions. A decrease in the minimal bactericidal concentration after irradiation was noted for one of the substances. In the cases of butynylthiobenzothiazolium hexabromotellurate, and tribromotelluromethylthiazoloquinazolinium bromide, decrease of both minimal inhibitory and minimal bactericidal concentrations in the irradiated inoculum was observed. The above shows that low-intensity laser radiation under certain parameters increases the susceptibility of microorganisms to antimicrobial agents.

Near-Infrared Light-Driving Organic Photothermal Agents with an 88.9% Photothermal Conversion Efficiency for Image-Guided Synergistic Phototherapy.

Photothermal agents (PTAs) with desirable near-infrared (NIR) absorption and excellent photothermal conversion efficiency (PCE) are ideal candidates for cancer treatment. However, numerous PTAs still require high-intensity and long-duration laser irradiation to completely ablate the tumor during the photothermal therapy (PTT) process, resulting in light damage to healthy skin and tissue as well as limiting their biomedical applications. Integrating intense NIR absorption and high PCE into a single small-molecule PTA is an important prerequisite for realizing efficient PTT, but is a serious challenge. Herein, a series of donor-acceptor type PTAs (CC1 to NC4) are designed through a molecular engineering strategy. Theoretical calculations and experimental results show that the NIR absorption and photothermal effect from CC1 to NC4 are significantly enhanced as expected. Notably, NC4 nanoparticles exhibit intense NIR absorption, superhigh PCE of up to 88.9% for PTT, photoacoustic imaging and photothermal imaging, and effective reactive oxygen species generation for photodynamic therapy (PDT). The superior PTT/PDT synergistic phototherapeutic efficacy is well demonstrated by the complete elimination of tumor in vivo upon one-time, low-intensity, and short-duration laser irradiation (808nm, 330mW cm-2, and 3min). This work provides a valuable guideline for rational design of PTAs for cancer phototherapy.

Acceptor-donor-acceptor type organic photothermal agents with enhanced NIR absorption and photothermal conversion effect for cancer photothermal therapy

Numerous photothermal agents (PTAs) require high-intensity and long-duration laser excitation for photothermal therapy (PTT), resulting in light damage to healthy skin and tissue as well as limiting their biomedical applications. Integrating desirable near-infrared (NIR) absorption and high photothermal conversion efficiency (PCE) into a single small-molecule PTA is an important prerequisite for realizing efficient PTT, but is a serious challenge. Herein, through molecular engineering strategy, an acceptor-donor-acceptor (A-D-A) type PTA (ADA3) was readily developed for 808 nm laser-driving photothermal imaging and PTT of tumor. Theoretical calculations and experiment results show molecular engineering strategy is significant in regulating the structure and energy gap of PTAs, so as to effectively induce a narrow band gap for NIR absorption and further optimize photothermal properties. ADA3 possesses molar extinction coefficient of 3.1 × 104 M−1 cm−1 at 808 nm, followed being assembled into nanoparticles, ADA3-NPs show high PCE of 80.3%. In vivo experiments indicate that ADA3-NPs have excellent antitumor capability under one-time, low-intensity and short-duration (808 nm, 330 mW/cm2, 3 min) laser irradiation. Therefore, this work definitely exemplifies the enormous potential of molecular engineering strategy and provides an effective method for developing small-molecule PTAs.

TRANSCRANIAL PHOTOBIOMODULATION IN NEUROLOGICAL AND PSYCHIATRIC PRACTICE: FUNDAMENTAL AND CLINICAL ASPECTS

Photobiomodulation is a relatively young medical technology that integrates the efforts of physicists, engineers and specialists in the field of biomedicine and includes various methods of light exposure to cells and tissues of the body. The main options for such effects are light radiation of the visible and IR spectrum generated by LED sources, and low-intensity laser radiation (LILI), which requires the use of lasers with special characteristics. In recent years, there has been an avalanche of interest among neurologists and psychiatrists in transcranial photobiomodulation, However, a systematization of empirical data and the fundamental foundations of technology is required. On this basis, the purpose of the review is to integrate ideas about the molecular-cellular and systemic effects of transcranial photobiomodulation and the prospects for its application in neurological and psychiatric practice. We have shown that intensive research in the field of studying the biological effects of low–intensity laser radiation, red and infrared light, which made it possible to decipher the main ways of responding to it at the molecular, cellular and tissue levels, created prerequisites for the formation of a new synthetic scientific direction - photobiomics. This translational discipline is focused on the development of pathogenetically sound technologies for the treatment of diseases of various profiles, including neurological and mental pathology. The results of experimental and clinical studies presented in the literature indicate the effectiveness of photobiomodulation in depression and post-traumatic stress disorder, nevertheless, extensive trials and meta-analyses are advisable for their full inclusion in international clinical recommendations.

The use of low-intensity laser radiation and liposomal cream in the treatment of inflammatory periodontal diseases in patients after the coronavirus infection

A number of authors put forward a hypothesis about the role of systemic subclinical vascular inflammation characteristic of COVID-19 in the development of peripheral complications of this infection, including in the oral cavity. Notably, there is a high incidence of gingivitis and periodontitis in patients after COVID-19. Material and methods. 35 patients diagnosed with chronic generalized periodontitis and chronic gingivitis in the acute stage were followed-up on an outpatient basis. All patients had a history of COVID-19 in the past 2 to 6 months. The patients were divided into two groups: the study group (n = 18) and the control group (n = 17). All patients underwent professional oral hygiene. In the study group, low-intensity laser therapy procedures were performed with preliminary application of a liposomal gel. Clinical research methods included taking an anamnesis, examination, and determination of indices (CFE index, Green Vermilion index, PMA index, CI index, Russell’s index). Research results. After therapy, all patients of the study group had grade 1 CFE index up to 4 (compensated); in the patients of the control group, grade 1–2 CFE was determined (compensated — in 88.2 % of the patients and subcompensated — in 11.8 %). After therapy, the Green Vermilion index decreased in both groups by 40.5 % and 31.3 %, with no significant intergroup difference. The PMA index decreased by 88.8 % in the study group and by 71.5 % in the control group. The index of bleeding reduced by 86.4 % in the study group and by 60.9 % in the control group. The Russell’s index, which allows taking into account the presence of gingivitis, tooth mobility, and the depth of the periodontal pocket, was also considered. Its value decreased by 92.3 % in the study group and by 63.8 % in the control group. Conclusions. The developed therapeutic complex, which includes low-intensity laser exposure with preliminary application of the liposomal gel, is more effective than the standard approach to the treatment of this pathology.

Evaluation of the results of complex medical rehabilitation of patients with polyposis rhinosinusitis in the long-term period

BACKGROUND: Previous studies on the comparative evaluation of the effectiveness of the course application of therapeutic physical factors during the complex medical rehabilitation of patients with polypous rhinosinusitis showed a rather high efficiency of the additional use of complex physiotherapy (alternating magnetic field and low-intensity laser therapy) in enhancing the regression of the main clinical manifestations of polypous rhinosinusitis and improving the quality of life (QoL) of patients. Considering the chronic and often recurrent nature of polypous rhinosinusitis, along with the relief of the main clinical manifestations of the disease and improvement in the quality of life of patients after treatment, medical rehabilitation of patients should also cover the long-term period.
 AIM: Evaluation of the results of complex medical rehabilitation of patients with polyposis rhinosinusitis in the long-term period (after 6, 12 and 18 months) and determination of the optimal time parameters for a repeated course of physiotherapy aimed at maintaining the achieved clinical effect and the level of quality of life of patients.
 MATERIALS AND METHODS: The study was performed with the participation of 120 patients with moderate to severe polypous rhinosinusitis and uncontrolled course. All patients were divided into 4 groups by simple fixed randomization. The first group (control, 30 patients) received only basic therapy, including endonasal application of Nasonex 2 doses 2 times a day and daily twice daily lavage of the nasal mucosa. Patients of the second group (comparison group 1, 30 patients) were additionally injected subcutaneously with dupilumab at a dose of 300 μg once every two weeks. In the third group (comparison group 2, 30 patients), against the background of the administration of dupilumab, exposure to an alternating magnetic field was performed. using the apparatus MAGNIT Med TeCo. Patients of the fourth group (main group, 30 patients), in addition to the volume of treatment of patients of the third group, received low-intensity laser radiation of the red spectrum (633 nm) using the AZOR-2K-02 apparatus.
 RESULTS: Positive dynamics in terms of assessed clinical indicators throughout the entire observation period was noted only in the comparison groups and the main group. The maximum severity of positive changes was determined in the main group after 6 and 12 months of rehabilitation, exceeding the achieved values in the group with dupilumab. The follow-up period made it possible to establish that 18 months after the start of rehabilitation, there were no significant differences between these groups. The dynamics of the parameters of the microcirculatory tissue system in the comparison groups and the main group in the long-term period indicated the formation of compensatory-adaptive processes aimed at eliminating congestion in the nasal mucosa and associated hypoxia, as well as the normalization of oxygen metabolism and trophism of tissues prone to chronic inflammation.
 CONCLUSION: The results obtained convincingly prove the effectiveness of additional course use of an alternating magnetic field in combination with low-intensity laser therapy. The use of this technology, implemented against the background of ongoing biological therapy, shows its increased efficiency up to 12 months after the start of medical rehabilitation. It is advisable to conduct a second course of physiotherapy based on the complex use of an alternating magnetic field and low-intensity laser radiation 9–12 months after the start of medical rehabilitation.

Prospects for the use of ozone therapy and low-intensity laser therapy in the treatment of atrophic pharyngitis

Improving the effectiveness of treatment of patients with atrophic pharyngitis through the use of ozone therapy and low-intensity laser therapy. A single-center experimental controlled randomized open-label study of 90 patients with AP aged 18 and over was conducted. All patients were randomly divided into three groups depending on the treatment performed: group I - traditional treatment method (rinsing the oropharynx with antiseptic solutions, the use of tablets for resorption), group II - treatment with ozone therapy (fine drip irrigation of the mucous membrane of the posterior pharyngeal wall with Ozonide oil), group III - treatment with ozone therapy and laser therapy. During the examination of patients, anamnesis collection, examination of ENT organs, cytological and microbiological examination of the mucous membrane of the posterior pharyngeal wall, contact endoscopy of the mucous membrane of the posterior pharyngeal wall were performed. 5-point visual analogue scales (VAS) were used to assess complaints and pharyngoscopic signs. Our results showed a statistically significant improvement in the quality of life of patients with AP (p=0.012), an improvement in the pharyngoscopic picture (p=0.003). The results obtained by us indicate an improvement in microcirculation under the influence of ozone therapy and low-intensity laser radiation. The technique using ozone therapy and low-intensity laser therapy is characterized by a bactericidal and fungicidal effect. There is a decrease in the total contamination of the posterior wall of the oropharynx with pathogenic and saprophytic microflora (there is a decrease in the contamination of the posterior wall of the pharynx with saprophytic and pathogenic microflora (p≤0.05), the differences are statistically significant). The technique using ozone therapy and low-intensity laser therapy has a pronounced anti-inflammatory effect, which was expressed in a decrease in the severity of dyskeratosis and hyperkeratosis. Thus, the use of ozone therapy in combination with laser therapy opens up new prospects for pathogenetically sound and effective treatment of AP.

Low-level laser therapy in multiple sclerosis: justification and optimization methods of application. (Literature review)

To analyze scientific data on the effectiveness and optimization of applying low-level laser therapy methods in patients with MS. Databases and libraries, namely PubMed, Scopus, ResearchGate, Google Scholar, J-STAGE, eLibrary.ru, were used for search. Publications of interest towards analysis of the ways of optimization of low-level laser therapy techniques and its effectiveness improvement, prospects for the development of this treatment method were selected. The total number of publications equal 87, mostly in English and Russian, was found. It was demonstrated, that low-level laser therapy can be considered a promising method of MS treatment. Mechanisms of therapeutic action of low-intensity laser radiation were shown, as well as the results of several clinical studies were presented. It was concluded that optimal values of all technique's indicators, namely wavelength, operation mode, power, frequency, exposure, etc., should be used. Laser blood irradiation (intravenous or external) and laser acupuncture, systemic techniques of low-level laser therapy are obligatory carried out with local irradiation of lesions.

Thermo-photobiomodulation of stem cells

The most important task of cell transplantology is to activate the proliferative potential of mesenchymal stem cells (MSCs) before receiving bone marrow cells from a donor. This is necessary to increase a sufficient number of MSCs in early passages, when the probability of chromosomal mutations is still low. The proliferative activity of cells can be activated using photobiomodulation (PBM) by exposure to lowintensity laser radiation in the visible and near-infrared ranges. Recently, it was shown in vitro that the combination of PBM and moderate laserinduced heating can lead to a significant increase in the efficiency of MSC colony formation. The main objectives of the study are to find the optimal parameters for such a combined effect and answer the question about the possibility of a synergistic effect of thermal heating and laser radiation. MSCs isolated from rat bone marrow were used for the experiments. MSCs were exposed to short-term laser radiation of moderate power with a wavelength of 980 nm and an energy density of 68-340 J/cm2, accompanied by moderate heating of the cell suspension. Vials with grown colonies were photographed, then their number, size and number of cells in individual colonies were determined using special digital image processing methods. It was found that under optimal parameters, exposure to laser radiation of moderate power leads to an increase in the number of colonies by 4.1±0.5 times, and the total number of cells by 3.3±0.4 times compared to the control. It has been shown that this increase in cell number occurs as a result of the synergistic effect of photobiomodulation and moderate heating. Activation of colony formation after laser stimulation of MSCs occurs due to the migration of cells from the initially formed colonies with the subsequent formation of additional colonies by separated cells.

Role of neodymium laser in surgery: stimulation of postoperative surgical wounds healing. Results of clinical studies

To prove the effectiveness of the low-intensity laser radiation application in the treatment of wounds of different origin. The clinical study involved 110 persons, divided into 55 subjects in both the study and control groups. The patients of the study group were exposed to the long-wave short-pulse neodymium laser immediately and within 35 days after interventions with a skin incision using it, in a way that wound treated with laser received low-level laser therapy. The control group patients' wounds were treated with standard methods by the means of topical drugs corresponding to the clinical manifestations of the wound process in each particular case. The study was carried out in the hospital of the department of maxillofacial and plastic surgery of the dental complex of the «Russian University of Medicine» from 2019 to 2022, and further conservative treatment was conducted in the department of dermatology and cosmetology of the University Hospital of the Medical Graduate School (Institute) of the RSSU. All wounds were classified into three groups for the convenience of systematization and formation of a generalized treatment protocol for postoperative surgical wounds. The objectivity and optimality of the chosen by us actions were confirmed in the conducted work. The formed scars were visually assessed on the POSAS scale at the end of the treatment by patients and 4 independent doctors, as well as each scar was visually assessed by four independent doctors and patients. At the end of the study we formed and proposed an algorithm for the treatment of surgical wounds of various origins. The parameters of the Aerolase Neo Light Pod neodymium laser for the treatment of patients with different types of skin wounds were clinically determined. Experimentally proven properties of the Aerolase Neo Light Pod neodymium laser on accelerating the healing process of surgical wounds through photobiomodulation mechanism support their regeneration with the formation of negligible normotrophic scars, as well as reduce the length of patients' treatment in surgical hospitals, as compared to patients receiving standard external drugs.

Evaluation of the effectiveness of complex medical rehabilitation of patients with polyposis rhinosinusitis associated with atopic dermatitis using ICF

BACKGROUND: In medical rehabilitation of patients with polyposis rhinosinusitis, aimed at solving the urgent strategic task of public health care to achieve and maintain an optimal level of functioning of persons suffering from partial disability through the development of effective methods of restoration of impaired functions and prevention of complications, there is a need to adequately assess the effectiveness of the measures taken. In this regard, it should be noted that the International Classification of Functioning, Disability and Health (ICF) serves as the gold standard for assessing the patient's quality of life and the effectiveness of rehabilitation measures. The category of human functioning acts as a system-forming basis and starting point of the patient's rehabilitation process in the ICF. In practice, the ICF is based on the biopsychosocial model of health (disease) assessment, which considers psychological and social factors of health (disease) and biological disorders in close interrelated unity. AIM: To evaluate the effectiveness of complex medical rehabilitation of patients with polyposis rhinosinusitis associated with atopic dermatitis using dynamic changes in the main domains of the ICF. MATERIALS AND METHODS: The study was performed with 89 patients with polyposis rhinosinusitis associated with atopic dermatitis. All patients were divided into 4 groups by simple fixed randomisation. The first group (control, n=22) received only basic therapy, including endonasal application of nasonex 2 doses 2 times a day, daily twice daily washing of the nasal mucosa, as well as the use of desensitising (antihistamines, topical glucocorticoids and moisturising and emollient agents) and detoxifying agents. Patients in the second group (comparison group 1, n=22) were additionally subcutaneously administered dupilumab at a dose of 300 mcg once a fortnight. In the third group (comparison group 2, n=23) against the background of dupilumab administration the combined effect of alternating magnetic field and low-intensity laser radiation of red spectrum was performed. Patients of the fourth group (the main group, n=22) received a course of phototherapy in addition to the scope of treatment of patients of the third group. The results of medical rehabilitation of patients with polyposis rhinosinusitis associated with atopic dermatitis were evaluated according to the dynamics of specialised categories of ICF, which most accurately characterise the patient's condition from the perspective of the biopsychosocial model of the disease. RESULTS: Changes in the estimated parameters of ICF at comparable initial values had different character and were determined by the scheme of medical rehabilitation. Minimal changes were recorded in the control group. The use of dupilumab was accompanied by positive dynamics of the parameters after 1 month with a significant increase in the shifts after 6 months, indicating the recovery of a number of mental and physical functions, as well as the ICF categories characterising the organism structures, activity and participation of the patient. Supplementation of biological therapy with course magnetolaser influence was accompanied by an increase in positive changes, which were confirmed in 6 months after rehabilitation. The maximum dynamics of the considered ICF categories was noted in the 4th group, where dupilumab administration was supplemented with complex use of therapeutic physical factors. CONCLUSION: The obtained results prove the high informativeness of the system approach realised with the help of ICF in assessing the effectiveness of medical rehabilitation measures. The comparative evaluation of rehabilitation results convincingly showed the advantages of the complex programme including dupilumab target therapy in combination with physiotherapeutic effects.

Effectiveness of Complex Medical Rehabilitation of Patients with Postthrombophlebitic Syndrome of Lower Extremities and Obesity: a Randomized Clinical Study

INTRODUCTION. The relevance of the development of comprehensive rehabilitation programmes for patients with postthrombophlebitic syndrome (PTPS) is due to the high frequency of disability and a significant reduction in their quality of life. Since obesity and excessive body weight are proven risk factors for the development and progression of chronic vein diseases, weight reduction is one of the important tasks of rehabilitation measures in patients with PTPS and concomitant obesity.
 AIM. Comparative study of the influence of complex methods of medical rehabilitation, including various methods of laser blood irradiation (supravascular and intravenous), pulse magnetotherapy and dry carbon baths, on the dynamics of indicators of composite body composition according to bioimpedance measurements in patients with postthrombophlebitic syndrome of the lower extremities and obesity.
 MATERIALS AND METHODS. A randomized prospective study was conducted on the basis of the Department of Medical Rehabilitation of Patients with Somatic Diseases of National Medical Research Center of Rehabilitation and Balneology of the Ministry of Health of Russia. The study included 40 patients with PTS of the lower extremities and accompanying obesity, the average age of which was 58.3 [51.5; 68.0] year), randomized to two groups. The patients of the 1st group received Intravenous laser blood irradiation (ILIB) (“Lazmik”, Russia), impulse magnetotherapy and dry carbon baths, as well as therapeutic gymnastics in the hall. The patients of the 2nd group received a complex that included supravascular laser irradiation of blood (“Azor-2K”, Russia), pulse magnetic therapy, and dry carbon baths, as well as therapeutic gymnastics in the gym. Anthropometric measurements (body mass, malleolar volume) were used for the evaluation of anti-edema and lipolytic effects after a comprehensive course of medical rehabilitation, as well as bioimpedance measurement of body composition (“MEDASS”, Russia).
 RESULTS. In patients with PTS of the lower extremities and obesity, after carrying out a course of medical rehabilitation using preformed physical factors, including various methods of laser irradiation of the blood (supravascular and intravenous), a significant decrease in body weight indicators was observed (p 0.001), a decrease in BMI (p 0.001), total and extracellular fluid (p 0.001) without significant differences between groups. A significant decrease in the index of fat mass (kg) (p = 0.007) was found in patients of the main group.
 DISCUSSION. Maintaining a healthy body weight as well as combating obesity are first-line secondary prevention measures recommended for all patients with PTS of the lower extremities. For the control of the dynamics of the composite composition of the body, an effective and sensitive method is the method of bioimpedance measurement, which allows to estimate the reduction of extracellular fluid (anti-flow effect) and fat mass of the body (lipolytic effect).
 CONCLUSION. The method of bioimpedance measurement, used for the evaluation of the composite composition of the body, showed high sensitivity and allowed to establish the greater effectiveness of the developed complex, which includes ILIB with a wavelength of low-intensity laser radiation (NILI) of 635 nm in reducing fat mass.

The influence of low-intensity laser radiation on the blood parameters of cows under technological stress

The study of clinical-hematologic parameters under technological stress at cows and the effect of low-intensity laser radiation (LILR) under technological stress was carried out. The study was carried out on high-yielding Holstein black-breed cows which were modeled technological stress and subsequently exposed to LILR on the withers or ear with exposure time of 5 or 15 depending on the group of animals. LILR with a wavelength of 830 nm was used. The study of hematological parameters of blood under technological stress in animals showed an increase in the number of leukocytes up to 14 days, a decrease in the number of erythrocytes and hemoglobin by 3 days of the experiment relative to the values of intact animals. At action of LILR under technological stress the increase of erythrocytes and hemoglobin in blood was registered. The content of leukocytes in blood corresponded to the level of intact animals, their functional activity increased. The concentration of reduced glutathione in animals under technological stress was decreased throughout the entire observation period. When using LILR under technological stress in animals, changes in reduced glutathione were less pronounced compared to animals after technological stress. When cows were exposed to LILR on the withers, an increase in the content of reduced glutathione in blood was registered. The obtained results indicate that the changes in blood parameters are directed to the activation of compensatory-adaptive reactions of the organism under the action of LILR under technological stress.

USE OF LOW-INTENSITY LASER RADIATION IN THE COMPLEX TREATMENT OF WOUNDS

Abstract. Increasing the eff ectiveness of treatment of infected wounds is an extremely urgent problem, which, despite signifi cant achievements, is far from a fi nal solution. The development of new methods of local impact on wounds, especially laser irradiation and evaluation of its eff ectiveness, are promising.The aim of the study. To evaluate the eff ectiveness of using low-intensity laser irradiation in the complex treatment of wounds.The object of the study was 10 patients with wounds of the lower extremities. Among the examined were 6 men and 4 women aged from 48 to 83 years. 6 patients had type 2 diabetes; in 4 – CHD, obliterating atherosclerosis of the vessels of the lower extremities. All patients involved in the study signed the appropriate informed consent.The complex treatment of all patients included irradiation of the wound with a low-intensity laser (B-Cure Laser); with a power of 250 mW; wavelength 808 nM; with a pulse frequency of 15 kHz; with a pulse duration of 17 μs with an energy of 3.75 Joule/min; with a spot of radiation 45Ч10 mm, lasting 3 minutes around the perimeter of the wound edges and 1 minute on the wound bed. The wound area was measured using the «IntimoMeasure» program. All patients underwent microbiological and cytological examination of smearsprints from the wound on days 1, 3, 5, 7 and 10.Research results. It has been established that the use of low-intensity laser irradiation of the wound has a positive eff ect on the wound healing process.As early as 2-3 days after the start of treatment, the amount of discharge from the wound signifi cantly decreased, the amount of necrotic tissue decreased, and the demarcation line was clearly defi ned. A decrease in the number of necrotic tissues and microorganisms, an increase in the number of leukocytes, monocytes, macrophages, and plasma cells was noted in the smears.After 4-6 days, signs of granulation formation appeared, the number of lymphocytes, macrophages, and plasma cells increased. The concentration of microorganisms did not exceed 10Ч2-3 CFU/ml. At 7-8 days, most of the wounds were without signs of necrotic tissue, granulation tissue increased intensively, signs of connective tissue formation, epithelization from the edges of the wound appeared.On 9-10 days, most of the wounds were represented by granulation tissue with pronounced proliferation of the epithelium at the edges of the wound. During the fi rst three days, the area of the wound decreased by almost 17 % compared to the initial size, and from the 7th day it did not exceed 60 % of the initial level.Conclusions. 1. Low-intensity laser irradiation of wounds against the background of complex drug therapy is an eff ective method of treatment, promotes rapid cleansing of wounds, intensive development of granulations, accelerates epithelization. 2. Availability, ease of use and absolute safety allow recommending this method for use in complex wound treatment.