Low Glucosinolate Content Research Articles

Envirotyping within a multi-environment trial allowed identifying genetic determinants of winter oilseed rape yield stability

Key messageA comprehensive environmental characterization allowed identifying stable and interactive QTL for seed yield: QA09 and QC09a were detected across environments; whereas QA07a was specifically detected on the most stressed environments.A main challenge for rapeseed consists in maintaining seed yield while adapting to climate changes and contributing to environmental-friendly cropping systems. Breeding for cultivar adaptation is one of the keys to meet this challenge. Therefore, we propose to identify the genetic determinant of seed yield stability for winter oilseed rape using GWAS coupled with a multi-environmental trial and to interpret them in the light of environmental characteristics. Due to a comprehensive characterization of a multi-environmental trial using 79 indicators, four contrasting envirotypes were defined and used to identify interactive and stable seed yield QTL. A total of four QTLs were detected, among which, QA09 and QC09a, were stable (detected at the multi-environmental trial scale or for different envirotypes and environments); and one, QA07a, was specifically detected into the most stressed envirotype. The analysis of the molecular diversity at QA07a showed a lack of genetic diversity within modern lines compared to older cultivars bred before the selection for low glucosinolate content. The results were discussed in comparison with other studies and methods as well as in the context of breeding programs.

Productive and qualitative profile of unexploited microgreen genotypes from Brassicaceae, Chenopodiaceae, Asteraceae and Portulacaceae families

Microgreens combine visual, taste, flavor and bioactive qualities based on genetic selection, making them a gastronomic novelty. In the present study, ten microgreen genotypes were investigated in terms of biometrical traits (fresh yield, dry matter concentration, and main color indices) alongside compositional analyses, involving cations, nitrate, vitamin C, phenols, and glucosinolate profile. The genotypes were selected from Brassicaceae (five), Chenopodiaceae (one), Portulacaceae (one) and Asteraceae (two) families, according to the availability of unexploited local varieties beyond the commercial ones. The microgreens were cultivated hydroponically in a controlled environment. Distinct genotypic variations were observed for each measured biometric and qualitative trait, with substantial differences noted between and within species. Among the ten genotypes, the underutilized purslane stood out for vitamin C (30 mg 100 g-1 f.w.), Mg (39 mg 100 g-1 f.w.), and the remarkably low nitrate content (7 mg 100 g-1 f.w.). White mustard exhibited the highest levels of glucosinolates (171 mg kg-1 f.w.), phenols (190 mg g.a.e. 100 g-1 f.w), and notable concentrations of cations such as potassium, calcium, and magnesium. From a nutritional perspective, ‘Mugnolo’ (Brassica oleracea var. italica Plenck) proved less suitable as a microgreen due to its highest Na/K ratio (2.28) and generally the low content of other minerals, phenols, vitamin C, and glucosinolates. The findings of this study hold significance for selecting new microgreen species/varieties that align with the preferences and requirements of both consumers and producers.

Comparative Study of Bolting Adaptability between 60Co-Induced Rape and Its Original Material

In southern China, the fresh shoots of rape are used as a high-quality seasonal vegetable owing to their pleasant taste. In this study, we investigated the taste and quality of fresh shoots of Fanmingyoutai, which was derived from WH23 by 60Co mutation. WH23 was used as a control (CK). Physiological indexes, transcriptome analyses, and metabolomics analyses between Fanmingyoutai and CK were studied and the related key differential genes were identified. The results showed that the glucosinolate content of Fanmingyoutai seeds was 51.14% lower than that of CK, and the contents of soluble sugar and vitamin C in the fresh shoots of Fanmingyoutai were 2.1 times and 1.4 times higher, respectively, than CK. Using transcriptome analyses, we identified that the differential genes were involved in glycan biosynthesis and metabolism, energy metabolism, carbohydrate metabolism, and the metabolism of cofactors and vitamins. Metabolomics analyses demonstrated that the contents of sucrose and D-fructose in the fresh shoots of Fanmingyoutai were 1.22 times and 1.15 times higher, respectively, than those in CK. Using qRT-PCR analyses, the expression of SWEET17, STP5, and GSL in the fresh shoots and leaves of Fanmingyoutai was two times higher than that in CK. SWEET17 (involved in sugar production and transport), STP5 (involved in monosaccharide transport), and GSL (involved in glucosinolate accumulation) may be the key functional genes. We concluded that the low glucosinolate content and high sucrose and D-fructose contents may be the main factors affecting the taste of fresh shoots of Fanmingyoutai and CK; SWEET17, STP5, and GSL may be the key related genes. This research provides a reference for the breeding and molecular mechanisms of new edible rape varieties.

Targeted editing of multiple homologues of GTR1 and GTR2 genes provides the ideal low-seed, high-leaf glucosinolate oilseed mustard with uncompromised defence and yield.

Glucosinolate content in the two major oilseed Brassica crops-rapeseed and mustard has been reduced to the globally accepted Canola quality level (<30 μmoles/g of seed dry weight, DW), making the protein-rich seed meal useful as animal feed. However, the overall lower glucosinolate content in seeds as well as in the other parts of such plants renders them vulnerable to biotic challenges. We report CRISPR/Cas9-based editing of glucosinolate transporter (GTR) family genes in mustard (Brassica juncea) to develop ideal lines with the desired low seed glucosinolate content (SGC) while maintaining high glucosinolate levels in the other plant parts for uncompromised plant defence. Use of three gRNAs provided highly efficient and precise editing of four BjuGTR1 and six BjuGTR2 homologues leading to a reduction of SGC from 146.09 μmoles/g DW to as low as 6.21 μmoles/g DW. Detailed analysis of the GTR-edited lines showed higher accumulation and distributional changes of glucosinolates in the foliar parts. However, the changes did not affect the plant defence and yield parameters. When tested against the pathogen Sclerotinia sclerotiorum and generalist pest Spodoptera litura, the GTR-edited lines displayed a defence response at par or better than that of the wild-type line. The GTR-edited lines were equivalent to the wild-type line for various seed yield and seed quality traits. Our results demonstrate that simultaneous editing of multiple GTR1 and GTR2 homologues in mustard can provide the desired low-seed, high-leaf glucosinolate lines with an uncompromised defence and yield.

Proximate Analysis and Antioxidant Properties of Young Plants of Sinapis alba L. Depend on the Time of Harvest and Variety

White mustard plant (Sinapis alba L.) is an easy-to-grow species with low soil requirements and is often sown as a catch crop in Northern Europe to reduce nitrate leaching, especially during the winter. There are studies showing the high nutritional value of mustard seeds, which have a wide application, mainly in food production. Still little is known about the young shoots or plants of different cultivars of white mustard, although in Asian countries, eating them raw is quite common. The aim of the research was to determine the proximate composition, antioxidant activity and polyphenolic compound content in young green plants of the Polish cultivars of white mustard: Borowska (traditional, with a high content of erucic acid and glucosinolates), Bamberka (non-erucic with glucosinolates) and Warta (non-erucic with low glucosinolates content; double-improved). Young plants were harvested in three terms. The first harvest took place at the plover stadium and the next ones at 7-day intervals (31, 38 and 45 day after sowing). In freeze-dried plant material, proximate composition and antioxidant activity with the ABTS and FRAP methods, as well as phenolic compound content, were measured. The highest concentration of protein was measured in cultivars Warta and Borowska after 31 and 38 days of sowing. Harvest time and cultivar affected antioxidant activity and total polyphenol content in young mustard plants. Thirty-eight days after sowing, the examined cultivars of the young plants of mustard had the highest antioxidant activity and total polyphenolic compound content. Green young mustard plants have strong antioxidant properties at the basic level, they are classified as functional foods and are similar to other edible leafy plants such as celery, spinach and Brussels sprouts.

Constitution of a Camelina sativa L. Synthetic Population and Agronomic Comparison between Spring and Winter Cultivation in North Italy

In recent years, the interest in increasingly sustainable agriculture has also turned attention towards new cover crops suitable for use in marginal areas that could enter the food chain as new protein and oil sources or for biodiesel production. In this scenario, Camelina sativa is a perfect crop to study. Camelina is an annual herbaceous plant belonging to the Brassicaceae which is interesting in terms of its oil content, since the seeds contain about 40% oils, with a high level of polyunsaturated fatty acids (30–40% alpha linolenic acid, 15–25% linoleic acid, 15% oleic acid and about 15% eicosenoic acid). It is a hexaploid species (2n = 40, genome size ~782 Mb) characterized by rapid growth, a short life cycle (85–100 days for spring varieties, 190–210 for autumn varieties) and low input cultivation needs. However, its use in feed and food is limited by the presence of glucosinolates (GLS). GLS are sulfur molecules involved in plant defense. In recent years, they have been studied not only as antinutritionals but also for their anti-carcinogenic effects against chronic inflammatory and heart diseases and for their use as natural pesticides. Given the recent interest in camelina and its highly nutritious oil, eight pure lines and a synthetic population were compared in two different growing periods, spring and winter. In this work, the genetic materials were characterized for different phenotypic traits, yields and yield components, and bromatological and glucosinolate content. The results confirmed that in North Italy, camelina has higher yields if cultivated in the autumn–winter period (about 2 t/ha vs. 0.6 t/ha); furthermore, a negative correlation was found between spring and winter yields, indicating that varieties that produce more in winter cultivation produce less in spring cultivation. Moreover, to our knowledge, it is the first work in which a synthetic population of Camelina sativa has been tested and proved to be a valid solution for use in various environments both for its adaptability and for the low content of glucosinolates (about 17 mmol/kg).

Bioactive Compounds Assessment in Six Moroccan Rapeseed (Brassica napus L.) Varieties Grown in Two Contrasting Environments

Rapeseed (Brassica napus L.) is the world’s second-largest oilseed crop after soybean. It contains functional compounds, including fatty acids and phenols, which are known for their health and nutritional benefits. In Morocco, rapeseed was introduced as a promising oilseed crop that has shown good adaptation and great potential. Six genotypes were registered and released as the most high-performance Moroccan varieties in terms of both seed yield and oil content. Apart from their ‘00′ quality, i.e., oil without erucic acid and meal with very low glucosinolate content, there is no information on other important quality traits. Therefore, this research aimed to characterize the lipochemical and phenolic attributes of those varieties, namely Baraka, Narjisse, Moufida, Lila, Alia, and Adila, grown at two contrasting sites (Allal Tazi and Douyet) so as to assess the environmental impact on oil quality. A set of 14 parameters were analyzed, comprising seed yield, oil content (Oil C), protein content (PC), acidity index (IA), peroxide index (IP), refractive index (IR), iodine value (IO), total phenolic content (TPC), total flavonoids, total carotenoid contents, free-radical scavenging activity (FRSA), half-maximal inhibitory concentration, fatty acid composition, and omega 6/omega 3 ratio. The results displayed significant differences (p &lt; 0.001) between the two sites for all the abovementioned traits, except for IA, with an outperformance of Allal Tazi over Douyet. Additionally, variety and variety by site interaction had a significant effect on all the studied parameters, except for IA, IP, IR, and TPC. Over both environments, the varietal effect generated variations in yield of 10.9 to 17.1 q/ha, Oil C of 36.7 to 39.9%, PC of 21.3 to 25.9%, IO of 94.1 to 100 g I2/100 g, FRSA of 22.7 to 42.9%, and an omega6/omega3 ratio of 1.8 to 2.3%. It is noteworthy that the Moufida and Alia varieties displayed a low-magnitude effect of the environment, as they maintained similar high performance over both sites. They, thus, represent Moroccan genetic material of interest from an agronomic and nutritional perspective. Therefore, they should be promoted and encouraged for cultivation in Morocco, mainly in the Allal Tazi area and similar regions.

Intraspecific and Interstage Similarities in Host-Plant Preference in the Diamondback Moth (Lepidoptera: Plutellidae)

The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), is an important insect pest of cruciferous crops. Understanding its preference patterns can lead to more efficient management methods, such as trap crops. Several strains of P. xylostella were used to test whether there were differences in oviposition preference in a four-choice setting, on abaxial versus adaxial leaf surfaces in 28 different plant species, and on substrates with different concentrations of sinigrin (allylglucosinolate). Additionally, the larval preference of P. xylostella was studied with 17 plant species of known glucosinolate content that were compared to Arabidopsis thaliana L. in two-choice tests. Our research shows that the diet on which P. xylostella has fed hardly affects multiple-choice host-plant preference, abaxial and adaxial oviposition preference, or oviposition response to pure glucosinolates. Our study also shows that glucosinolate content affects larval preference, which together with the known correlation between glucosinolate content and P. xylostella oviposition, indicates that crops with high glucosinolate content could be more susceptible to damage by P. xylostella than crops with low glucosinolate content. These findings are discussed in regards to their significance in the management of P. xylostella.

Metabolism Reorganization in Kale (Brassica oleracea L. var acephala) Populations with Divergent Glucosinolate Content under Thermal Stresses

Thermal stress causes the reduction in productivity and harvest quality. To adapt to different temperature ranges, plants activate protecting metabolic pathways. Previous studies have reported that stressful environments due to abiotic stresses have an impact on the accumulation of glucosinolates (GSLs) in Brassicaceae plants. In order to determine the role of GSLs in the plant response to thermal stress, we conducted a study comparing four populations with a high and low GSL content. The GSL levels were analysed at different temperatures [control (20), 12 and 32 °C], detecting that populations with a higher GSL content increased their resistance to the cold. In addition, populations subjected to the cold increased the content of indolic GSLs. Populations with high levels of GSLs show higher levels of glucobrassicin (GBS) and sinigrin (SIN) under cold temperatures than plants grown under control conditions. High temperatures have a lower impact on GSLs accumulation. To elucidate the induced metabolic changes due to the accumulation of GSLs under cold conditions, we performed an untargeted metabolomic analysis and identified 25 compounds differentially expressed under cold conditions in the populations with a high GSL content. Almost 50% of these compounds are classified as lipids (fatty amides, monoradylglycerols, diterpenes, glycosylglycerols, linoleic acids and derivatives). Organoheterocyclic and nitrogenous organic compounds are also over-represented. Therefore, the current results suggest that GSLs play a key role in cold tolerance. Although the associated molecular mechanisms have not been elucidated, the non-targeted metabolomics assay shows a significant change in the lipid profile, with compounds that need to be studied further.

Сорт сурепицы яровой Медовая тип «000»

The variety of spring turnip rape Medovaya is developed by a method of multiple individual selections from the variety Zolotistaya of VNIIMK breeding. In a competitive trial, the new variety exceeded the standard variety Zolotistaya by seed yield in average for three years by 0.21 t/ha. The variety Medovaya is distinguished with higher oil content in seeds exceeding the standard one by 0.3%. Also the new variety have low glucosinolate content in seeds – 13.5 µmol/g. Protein content in seeds of the variety Medovaya is similar to the same trait of the standard variety and equal to 23.72%. However, the summarized oil and protein contents in seeds of the new variety is higher by 1.9% comparing to the standard. The seeds of the variety Medovaya have homogenous yellow color. Thousand-seed weight is 3.0 g and is equal to the standard. The fatty-acid composition of oil corresponds to the demands to salad oils of high quality, having erucic acid content of 2.0%. Oil from the seeds of the variety Medovaya has optimal rate of ɷ-9 : ɷ-6 : ɷ-3 for the health human nutrition. A duration of a growing season of the new variety is 77 days; it is not differed of the standard variety. The short growing season allows cultivating spring turnip rape in regions where the other oil crops cannot realize their potential. So this crop can be a possible reserve to complete the resources of highly qualitative oil and fodder protein.

A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots.

Radish (Raphanus sativus L.) is rich in specific glucosinolates (GSLs), which benefit human health and special flavor formation. Although the basic GSLs metabolic pathway in Brassicaceae plants is clear, the regulating mechanism for specific glucosinolates content in radish fleshy taproots is not well understood. In this study, we discovered that there was a significant difference in the GSLs profiles and the content of various GSLs components. Glucoraphasatin (GRH) is the most predominant GSL in radish taproots of different genotypes as assessed by HPLC analysis. Further, we compared the taproot transcriptomes of three radish genotypes with high and low GSLs content by employing RNA-seq. Totally, we identified forty-one differentially expressed genes related to GSLs metabolism. Among them, thirteen genes (RsBCAT4, RsIPMDH1, RsMAM1a, RsMAM1b, RsCYP79F1, RsGSTF9, RsGGP1, RsSUR1, RsUGT74C1, RsST5b, RsAPK1, RsGSL-OH, and RsMYB28) were significantly higher co-expressed in the high content genotypes than in low content genotype. Notably, correlation analysis indicated that the expression level of RsMYB28, as an R2R3 transcription factor directly regulating aliphatic glucosinolate biosynthesis, was positively correlated with the GRH content. Co-expression network showed that RsMYB28 probably positively regulated the expression of the above genes, particularly RsSUR1, and consequently the synthesis of GRH. Moreover, the molecular mechanism of the accumulation of this 4-carbon (4C) GSL in radish taproots was explored. This study provides new perspectives on the GSLs accumulation mechanism and genetic improvements in radish taproots.

Assessment of oil quality traits in some important exotic and indigenous collections of

Context In the breeding of oilseed Brassica crops for human consumption and animal feed, the main objective is the expansion of commercial varieties with low erucic acid, low glucosinolates, and high oleic acid content. Indian cultivars are high in erucic acid and glucosinolates. Aims This study examined variation among species and genotypes of Brassica for early screening potential in breeding programs for enhanced oil quality of rapeseed mustard. Methods Seeds of 165 Brassica genotypes including exotic and indigenous collections from India were analysed for oil content, fatty acid composition of seed oil, and glucosinolate content in seed meal. Species represented were B. juncea (159 genotypes), B. rapa (2), B. napus (2), B. carinata (1) and Eruca sativa (1). Correlation, principal component and cluster analyses were performed. Key results Substantial genetic variability was detected among genotypes for all studied traits. Oil content ranged from 32% to 45%. Oleic (11.93–54.13%), linoleic (13.33–35.85%), linolenic (3.42–20.77%) and erucic (0–50.70%) acids were the dominant fatty acids. Erucic acid had a significant and negative association with oleic, linoleic, linolenic and eicosenoic acids. Glucosinolate content varied from 7.32 to 282.17 μmol/g in the defatted meal. The first two principal components accounted for 48.10% of cumulative variation. Genotypes were grouped into five major clusters. Genotypes of one cluster had high oleic acid (46.73%) and low erucic acid (1.72%). Conclusions Five promising genotypes were found with low glucosinolate (&lt;30 μmol/g defatted meal) and low erucic acid (&lt;2%) content (i.e. ‘00’ characteristics). Implications The inherent variation for seed oil quality traits across assessed Brassica genotypes suggests their potential for application in future breeding operations.

Селекция рапса озимого во ВНИИМК: история и новые результаты (обзор)

Breeding of winter rapeseed in the V.S. Pustovoit All-Russian Research Institute of Oil Crops was started at the end of 60th of previous century with a selection of perspective winter forms from hybrids of spring brown mustard and winter rapeseed. Heterosis in intraspecific hybrids was studied. The best intraspecific hybrids exceeded the parental forms by 20–36% by seed yield, by 20–27% by green mass yield. A possibility to create intervarietal hybrids based on heterostyly was studied. Conditions for plants acclimation to increase cold resistance of winter rapeseed were studied; methods of selection for cold resistance were developed. In 1982, the development of rapeseed and turnip rape cultivars of ‘00’ type began. To create diversity of breeding germplasm, a great amount of samples from European counties that actively deals with rapeseed breeding were included into the work. In the V.S. Pustovoit All-Russian Research Institute of Oil Crops, the methods of estimation of oil and fodder protein quality were perfected, cultivars of rapeseed and turnip rape of ‘00’ type (e.g. erucic acid free and with low glucosinolate content) were developed. As a result of breeding for oil quality, high oleic cultivars were developed, that not only improve upon the oil nutritive efficiency but open new possibilities of its technical uses due to increasing oxi- and thermostability. The first Russian high oleic winter rapeseed cultivar Olivin was developed in the V.S. Pustovoit AllRussian Research Institute of Oil Crops and introduced in the State Variety Commission in 2019. The linear cultivars of winter rapeseed were developed by inbreeding, the first interlinear hybrids – using a CMS Ogura system. One of them named Debyut was introduced in the State variety trials 2020.

Высокопродуктивный сорт сурепицы яровой тип «000» Грация

The spring turnip rapeseed cultivar Gratsiya was developed at the Siberian experimental station – a branch of V.S. Pustovoit All-Russian Research Institute of Oil Crops by multiple individual and family selections from high-yielding, yellowseeded forms of the German cultivar Eldorado. In 2020, we isolated elite plant in the selection nursery by the main agronomic valuable traits: early maturity, high productivity, resistance to lodging, yellow seed color, low content of glucosinolates in the seeds and erucic acid in the oil. According to the results of three years competitive trials (2018–2020), 12 promising numbers with a growing season of 66–70 days were evaluated and a promising high-yielding number 31119 – the cultivar of type “000” Gratsiya was isolated. The cultivar of spring turnip rapeseed Gratsiya exceeded the standard cultivar Pobeda in seed yield by 0.21 t/ha and in oil yield by 0.08 t/ha. By the main agronomic traits, the cultivar Gratsiya differs from the standard one in lower content of glucosinolates in seeds (14.6 µmol/g) and erucic acid in oil (0.43 %). Seedlings are green, without wax coating, the plant is semi-dense, medium-branched, plant height of 120– 135 cm. The fruit is a pod, 5–7 cm long. Seeds are yellow, with an addition of brown – up to 2 %. Thousand-seed weight is 2.5–2.8 g. It is moderately drought-resistant, has uniform ripening, is suitable for mechanical harvesting, and is recommended for cultivation throughout the Russian Federation because of its early maturity.

Efficacy of Different Forms of Green Manure Crops to Reduce Verticillium dahliae in Different Soils

The efficacy of green manure crops to reduce the number of Verticillium dahliae microsclerotia in different soils was investigated. Green manures tested were Indian mustard with a high glucosinolate content and sorghum-sudangrass as biocidal plants, and Indian mustard with a low glucosinolate content and rye as non-biocidal plants. The green manure plants were applied in fresh, dried, and ensilaged form. When applied as fresh plants, the glucosinolate content determining the biocidal activity of Indian mustard was only important in loam soil but not in sandy loam soil. In the latter soil, the non-biocidal rye had significantly higher efficacy than the Indian mustard. Volatiles released by fresh and dried, but not ensilaged, Indian mustard with a high glucosinolate content strongly decreased the number of living V. dahliae microsclerotia. When the same green manure crops were added to sandy loam and clay loam soil, the effect of the high glucosinolate content Indian mustard in fresh and dried form disappeared, whereas the ensilaged green manure crops had the highest efficacy. This effect was based on the increase of the soil microbial activity and the Streptomyces population size, which were negatively correlated with the number of living V. dahliae microsclerotia in the soil.

Breeding of spring turnip rape, Brassica rapa L. var. subsp.campestris (L.) A.R Clapham at All-Russian Research Institute of Oil Crops

Turnip rape, Brassica rapa L. var. sabsp. Campestris (L.) A.R.Clapham belongs to the large cabbage family (Brassicacea). Spring turnip rape among other oilseeds of the Brassicacea family occupies an important place and can be highly productive in the Northern regions of the European part, in the arid zones of the Lower Volga region, in the northern forest-steppe and subtaiga regions of Western and Eastern Siberia, where the longer growing season sometimes hinders rapeseed to achieve its full potential. Spring turnip rape ripens 13-15 days earlier than rapeseed; it is less heat- and drought-sensitive. However, interest in industrial cultivation of spring turnip rape appeared in the 70s of the last century after the creation of non-erucic and low-glucosinolate varieties, with the oil equivalent to olive oil in its fatty acid composition. Spring turnip rape has been bred at V.S. Pustovoyt All-Russian Research Institute of Oils Crops (VNIIMK) since 1982. The yellow-seeded varieties, Vostochnaya, Yantarnaya, and Zolotistaya, were created. The objective of this research was to find a new promising breeding material for creating varieties of 000-type spring turnip rape from heterogeneous populations of the available VNIIMK varieties. To obtain a new source material, we used the pedigree method, followed by an assessment of the progeny of elite plants isolated from Yantarnaya and Zolotistaya. The result of our six-year efforts was four new promising samples No. 815, 844, 816, and 820, which exceed the standard variety, Zolotistaya, in seed yield by 10-14%. The oil content of seeds of the isolated samples varied from 47.8 to 48.6%, and exceeded the standard variety by 0.3–0.7% in three of them. Promising cultivars either had the same or lower weight of 1000 seeds (2.4-3.0 g), in comparison with Zolotistaya. All selected samples of turnip rape were characterized by a low glucosinolate content in seeds – 13.5–14.5 μmol g-1, and the fatty acid composition corresponded to the requirements for high-quality salad oils with erucic acid levels of 0.04–0.20% and a high content of oleic acid – 65.8–67.4%. After additional assessment, the best variety will be submitted for the State test.

Canola quality by handheld NIR spectrometer

Handheld NIR spectrometers can be used to predict some of the most important compounds that define canola quality (high oil and protein contents, low glucosinolate and chlorophyll contents, and fatty acid composition). It is important to test models on true external verification sets instead of relying only on cross-validation for model stability. For handheld NIR spectrometers, the compact design necessarily has small sample windows, necessitating multiple measurements in intact seeds to reduce sampling error. Ground/milled seeds or flour samples have lesser sampling effect. It is important to understand how the limited wavelength range of some handheld NIR spectrometers makes NIR spectrometers unsuitable for the prediction of certain quality parameters.

НОВЫЙ СОРТ ЯРОВОГО РАПСА ВЕЛЕС

The results of breeding a promising variety of spring rapeseed 'Veles' are presented. The variety was created using chemical mutagenesis. The seeds of the 'Vikros' variety were treated with ethylmethanesul-fonate at a concentration of 0.03%, followed by the selection of lines characterized by early maturity, re-sistance to lodging, and improved biochemical composition of the seeds. The line № 834-16 is distin-guished by the accelerated development, the changed architectonics of the plant. The growth of lateral shoots begins from the base of the main shoot, the lower internodes are shortened, which increases the resistance of plants to lodging. Side shoots of the first order begin to bloom almost simultaneously with the main shoot. Flowering and maturation of seeds on shoots of different orders takes place simultaneous-ly with the central one, which contributes to the friendly maturation and equalization of the obtained seeds. Seeds are characterized by an increased fat content, contain less fiber. The content of crude fat va-ries from 47.4 to 48.8%, crude protein — 19.7–24.4%, crude fiber — 6.52–7.62%. In the composition of fatty acids, the content of valuable oleic acid is 7.15% higher than of standard. The average seed yield for 3 years of testing is 3.28 t/ha, which is 0.64 t/ha higher than the standard. The line was transferred to the State Commission for Variety Testing and Protection of Breeding Achievements under the name 'Veles'. The variety is resistant to lodging. Guarantees the production of 28–32 t/ha of green mass with a yield of up to 6 t/ha of dry matter. The low content of glucosinolates in seeds (11.6–13.9 mmol/g) will allow the use of seeds, cake and meal when feeding animals in high doses. The variety is approved for use from 2021 in 4 regions of the Russian Federation: North-West, Central, Ural and West Siberian.

Новый сорт рапса ярового Сибиряк 60

In 2020, we submitted the spring rapeseed variety Sibiryak 60 bred at the Siberian Experimental Station (branch of V.S. Pustovoit All-Russian Research Institute of Oil Crops) to the State Commission of the Russian Federation on testing and protection of the selection achievements. We developed the variety by the method of multiple individual-family selection from the variety Avangard (All-Russian Research Institute of Rapeseed, Lipetsk). In 2012, there was identified an elite plant and further breeding work was carried out to improve the main economic traits: early maturity, productivity, oil content, resistance to lodging, seed falling and to the main pathogens. The general characteristics of the variety are high seed productivity and low content of glucosinolates in seeds. According to the results of the competitive variety trial (2018–2020), the spring rapeseed variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.29 t/ha and in oil yield by 0.12 t/ha. In the conditions of the Krasnodar region, the variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.31 t/ha, in the conditions of the Lipetsk region – by 0.30 t/ha. The new variety is meant to for cultivation for seeds in the Volgo-Vyatka (4), Middle Volga (7), Ural (9), West Siberian (10), and East Siberian (11) regions. The originator of the variety Sibiryak 60 is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

Сурепица озимая — значение, использование в кормопроизводстве

Presents literature review on the value of biological characteristics, the use of winter turnip rape (Brassica campestris fr. biennis). Brassica campestris fr. biennis is inferior to winter rapeseed in seed productivity, but due to the lower location of the growth point in autumn, it has a potential resistance to freezing. Pods of winter turnip rape, unlike rapeseed, do not crack under adverse weather conditions of harvesting and overstocking on the root. Seed production of winter turnip rape is 1.5–2 times higher than spring. It is characterized by a shorter (10–20 days) growing season compared to winter rapeseed. It requires a smaller amount of effective temperatures in the autumn period, so it is sown 1–2 weeks later than winter rapeseed. Winter turnip rape is the earliest forage crop. It is used in multicomponent mixtures with triticale and vetch for green food. Early harvesting allows you to thoroughly prepare the soil for intermediate and subsequent crops in the crop rotation. As a part of the mixture, it can form more than 32 t/ha of green mass with an output of up to 7 t/ha of dry matter, or up to 5900 feed units and 0.7–1.1 t/ha of raw protein. The preparation of silage feed from winter turnip rape provides high-energy feed with an exchange energy content of 10.51 MJ/kg of dry matter. Both in pure form and in a mixture allow creating a green feed conveyor. It is good honey plant. Seeds and meal of turnip rape have a higher feed value due to the low content of fiber, lignin, glucosinolates and other undesirable substances in them and can be successfully used to compound feeds with protein. The grade of winter turnip rape 'Zarya', created in the Federal Williams Research Center of Forage Production and Agroecology is used for forage and sideration purposes both in the main and in intermediate sowing. The variety is intended for use on seeds for the production of oil for both food and technical purposes, as well as for use in the green raw material conveyor. The seeds contain 23.8 ± 2% crude protein and 43.9 ± 2.3% crude fat. It is characterized by the absence of erucic acid in the oil, the content of crude fiber in the seeds is 6.41 ± 1.54%. The average content of glucosinolates in seeds is 13.2 mmol/gram. The proportion of essential amino acids in fat-free matter of seeds of winter turnip rape dawn is 46.6–50.1%, of crude protein 37.6 to 40.1%. The amino acid index (the ratio of essential amino acids to non-essential ones) is quite high: 0.8–0.9.