Automobile-robot (self-driving automobile) is being researched and developed vigorously. When the automobile-robot is moving on the road surface, the low frequency vibration excitation not only influences the ride comfort of the automobile-robot but also strongly affects the durability of the vehicle’s structures. To research the automobile-robot’s vibration in the low frequency region, a dynamic model of the vehicle is established to calculate the vibration equations in the time region. Based on the theory of the Laplace transfer function, the automobile-robot’s vibration equations in the time region are transformed and converted to the vibration equations in the frequency region. Then, the effect of the design parameters and operation parameters on the characteristic of the automobile-robot’s acceleration-frequency is simulated and analyzed to evaluate the ride comfort as well as the durability of the automobile-robot’s structures in the frequency region. The research results show that the design parameters of the stiffness, mass, and road wavelength remarkably affect the characteristic of the automobile-robot’s acceleration-frequency. To reduce the resonant amplitude of the acceleration-frequency in the vertical and pitching direction of the automobile-robot, the stiffness parameters of the automobile-robot's and tires should be reduced while the mass of the automobile-robot’s body should be increased. Additionally, the road’s roughness also needs to be decreased or the road’s quality needs to be enhanced to reduce the resonant amplitude of the automobile-robot’s acceleration-frequency.