Low-fat Sausages Research Articles

락토페린을 첨가한 저지방/저염 소시지의 품질 및 Escherichia coli O157:H7에 대한 항균 활성에 미치는 효과

기능성 식육제품을 제조하기 위해 첨가한 락토페린의 항균활성을 평가하기 위해 Escherichia coli O157:H7을 일정 수준으로 접종하고 락토페린을 0에서 1%로 달리 첨가한 결과, 농도에 의존적으로 항균활성을 나타냈으며, 특히 0.25% 이상의 농도에서는 접종균에 대한 뚜렷한 항균효과를 나타냈다. 이러한 결과를 근거로 소시지의 저장성 증진을 위해 락토페린을 첨가하여 제조하였으나 저장성 실험 결과에서는 항균효과가 확인되지 않았다. 또한 젖산나트륨을 2% 첨가하고 락토페린을 0.3에서 0.9%로 복합 첨가하여 4℃ 저장 중에 접종한 E. coli O157:H7에 대한 항균활성을 측정한 결과에서는, 젖산나트륨 2% 단독 첨가구는 4주 이후 접종균의 생장이 억제되었으나 젖산나트륨과 락토페린을 복합첨가한 첨가구는 락토페린의 농도가 증가할수록 저장기간 동안 접종균의 성장으로 항균효과가 오히려 감소하였다. 따라서 모델결과에서 보여준 항균효과는 실질적인 식육제품실험에서는 나타나지 않았으며, 이러한 결과는 제조 및 가열 중에 락토페린의 변성과 소시지 제조에 첨가되는 복합첨가물들의 영향으로 첨가된 락토페린의 항균활성이 저해된 것으로 판단된다. 차후의 연구로 락토페린의 항균활성을 높이기 위하여 케이싱의 내면에 필름으로 처리하는 방법으로 항균활성효과를 연구하는 것이 필요할 것으로 사료된다 This study was performed to determine potential antimicrobial activity of lactoferrin, which incorporated into the low-fat/salt sausages during storage either at 30 or 4℃. First, the model study on the antimicrobial activity of lactoferrin was performed. Based on the model study, more than 0.25% of lactoferrin was required to have a distinctive antimicrobial activity for the growth of E. coli O157:H7 in the broth. However, extended shelf-life was not shown in the low-fat/salt sausages manufactured with lactoferrin during refrigerated storage. In addition, no synergistic effects of lactoferrin with sodium lactate were observed in the sausages. These results indicated that the addition of lactoferrin into the low-fat sausages did not have antimicrobial activity of E. coli O157:H7, due to the denaturation of lactoferrin or the complexity of the sausage, even though it had a distinctive antimicrobial effect in the model study.

아질산염의 대체제로 고분자 키토산의 첨가가 저지방 소시지의 냉장 저장 중 이화학적 성상 및 미생물의 변화에 미치는 영향

본 실험은 아질산염의 대체제로서 키토산과 젖산나트륨을 이용하여 저장성과 발색도 효과를 조사하기 위해 실시하였다. 그 결과 저장성에서는 아질산염의 대체제로서 젖산나트륨과 비교하여 저장 기간 중 차이점은 나타나지 않았지만 색도에서는 아질산염을 첨가한 시료에 비해 키토산과 젖산나트륨을 첨가한 시료의 적색도가 많은 차이를 보임으로써 키토산에 의한 아질산염의 완전대체는 불가한 것으로 판단된다. 하지만 접종한 Listeria monocytogenes는 아질산염 및 젖산나트륨과는 유사한 항균효과를 보여주고 있어서 키토산의 항균효과는 나타난 것으로 평가되며 앞으로 아질산염을 대체할 수 있는 천연소재의 개발이 필요할 것으로 판단된다. This study was performed to investigate if high molecular weight(～200kDa) of chitosan can be a potential possibility to replace with the sodium nitrite in low-fat sausages. pH, proximate analysis, Hunter color values, vacuum purge, expressible moisture(EM, %), texture profile analysis(TPA), shelf-life effect were measured. No differences in physico-chemical properties were observed between controls and chitosan treatments(p>0.05). Since Hunter-a-values(redness) were reduced with the addition of chitosan as compared to the sausages containing sodium nitrite and sodium lactate, it could not be replaced by chitosan completely. However, total plate counts(TPC), thiobarbituric acid reactive substance(TBARS), volatile basis nitrogen(VBN) did not differ between chitosan treatments and controls. These results indicated that the addition of chitosan into meat products would be replaced with sodium nitrite partially, but it may not be completely replaced due to the reduced Hunter-a-values. Further research will be continuously performed to screen the natural ingredients which might have a cured pigment in meat products.

Application of by-products from industrial processing of potato flour and yellow peas as ingredients in low-fat high-fibre sausages

Experiments using dry potato pulp from processing of potato flour, and pea flour from processing of yellow peas as raw materials for processing of low-fat high-fibre sausages were carried out. Color and texture were measured using objective methods and the approximate content of energy was calculated. A 1:1 mixture of potato pulp and pea flour hydrated with 7:1 vegetable soup resulted in sausages with a delicious flavor and texture and an attractive external and internal color. The percentage of fat energy was reduced from 68% in the standard sausages to 24% in the low-fat high-fibre sausages. Factor analysis showed that 96% of the data variation in results from measurement of texture could be attributed to factors of high loadings for hardness, energy, modulus and gumminess. Cohesiveness, modulus, energy, hardness and gumminess were the most important factors. Results from studies of the effect of exchange of meat with water showed patterns similar to the pattern between texture characteristics and dry matter. It was theorized that hardness depended on two gelling processes. The texture patterns were associated to salt and dry matter.

Evaluation of low-fat sausage containing desinewed lamb and konjac gel

Ground (GR) or desinewed (DS) lamb trimmings were combined with a konjac flour (KF) gel (0%, 10% or 20%) to produce a low-fat (8%) cured sausage. Physiochemical, sensory and shelf-life analyses were performed to determine the efficacy of the fat mimetic. Desinewing reduced collagen content (2.3 mg/g tissue) and cook yield (0.6%) when compared to GR trimmings. Grinding increased aerobic plate counts (APCs) ∼0.4log 10/cm 2 more than DS, but APCs were not affected by inclusion of KF. Sausages with 20% KF lowered cook yield ∼1% and slightly reduced sensory panel and texture profile analysis texture values. KF gel incorporated at 10% had similar properties to a control low-fat desinewed lamb sausage, while KF at 20% could reduce “toughening” in low-fat sausage products. Use of konjac gel as a fat mimetic could reduce total caloric energy by replacing a portion of the meat in a sausage formulation.

Growth Suppression of Inoculated Listeria monocytogenes and Physicochemical and Textural Properties of Low‐fat Sausages as Affected by Sodium Lactate and a Fat Replacer

ABSTRACT: The antilisterial effect of low‐fat sausages (<2%) manufactured with a fat replacer and increased level of sodium lactate (SL, 60%) solution was determined during refrigerated storage after inoculation with 103 colony‐forming units/g of Listeria monocytogenes (LM) at the initial storage. The fat replacer prevented moisture loss during cooking or storage. Increased SL in combination with the fat replacer resulted in lower water activity, total plate, and LM counts. In addition, whiteness values decreased (P < 0.05), but yellowness values increased (P < 0.05) with increased SL level. During storage, low‐fat sausages containing at least 3.3% SL and the fat replacer had greater antilisterial effect than the low‐fat control.

Utilization of Transglutaminase for the Development of Low-fat, Low-salt Sausages and Restructured Meat Products Manufactured with Pork Hams and Loins

This study was performed to determine whether transglutaminase (Aciva-TG, TGase) can be used to reduce the salt level in low-fat sausages and to replace emulsified meats (10%) for the manufacture of restructured meat products (RMP). Pork hams and loins were collected from a local retail market in Gwangju, Korea and used for the manufacture of sausages and RMPs, respectively. TGase at the level of 0.1% can permit the reduction of the salt level from 1.5% to 1.0% in low-fat comminuted sausages without any quality defects, however a crumbly texture was found if the salt level was reduced below 1.0% even though it combined with certain amounts of TGase. No differences in chemical composition and physical properties were observed (p>0.05) among treatments. Approximately 0.3% of TGase can replace 10% emulsified meats, which are normally used for improvement of binding capacity to manufacture RMPs, without quality defects. This study suggests that TGase could be used for the manufacture of low-fat, low-salt functional meat products for the improvement of textural characteristics and binding capacity without adverse effects. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 2 : 261-265)

식육단백질과 친수성 콜로이드의 상호결합 특성을 이용한 저지방 육제품 제조기술 개발 - II 모델연구결과를 이용한 저지방 소시지의 개발

본 연구는 유화형 소시지와 유사한 물성을 갖는 저지방 소시지를 제조하기 위하여 실시하였다. 이전의 모델연구결과를 통해 얻은 최적조건을 저지방 육제품 제조에 적용하였으며 제조한 저지방 소시지의 pH는 6.29～6.34, 수분 74～76%, 지방＜3% 및 단백질 15～18%인 반면, 유화형 소시지는 pH 6.51, 수분 56%, 지방 26.9% 및 단백질은 13.2%이었다. 친수성 콜로이드를 단독으로 첨가한 저지방 처리구는 무첨가구와 비교할 때 가열수율, 보수력 및 색도에 영향을 미치지 않았으나, CN을 첨가한 처리구의 보수력은 유화형 대조구와 KF 및 LBG를 각각 단독으로 첨가한 처리구에 비하여 오히려 낮았다. 물성특성에서는 저지방 대조구와 CN을 첨가한 저지방 처리구가 유화형 대조구보다 탄력성 및 응집성이 높았고, KF와 LBG를 각각 첨가한 처리구는 부서짐성과 경도 및 탄력성이 낮았다. KF, CN 및 LBG의 2종 내지 3종을 복합으로 첨가하여 저지방 소시지를 제조하였을 때 유화형 대조구에 비하여는 보수력은 떨어졌으나, KF와 LBG(KLL) 및 KF와 CN과 LBG(KCL)를 복합으로 첨가한 처리구는 단독 첨가구에 비하여 보수력이 증진되었다. 물성적인 특성에 있어서는 KF와 LBG를 혼합하여 첨가한 처리구를 제외하고 다른 혼합 첨가구는 유화형 대조구와 비교적 유사한 물성적인 성상을 가졌으며 그 중 KF와 CN의 복합처리구가 유화형 대조구와 가장 유사한 물성을 가졌다. 이상의 결과에서 볼 때 기존의 유화형 대조구와 유사한 물성을 갖는 저지방 소시지를 개발하기 위하여 친수성 콜로이드의 단일첨가보다는 보수력이 좋은 KF 혹은 LBG 등과 경도를 높여 주는 CN과 의 복합지방대체제의 첨가가 더욱 효율적임을 알 수 있었다.

Effect of fat level and partial replacement of pork backfat with olive oil on processing and quality characteristics of fermented sausages

Six formulations of dry fermented sausages were produced in three replications with three initial fat levels (30, 20 and 10%) and two levels (0 and 20%) of pork backfat replacing olive oil. After 4 weeks of fermentation and ripening the fat content of the treatments with 30, 20 and 10% fat level ranged from 38.86 to 43.60%, 25.56 to 26.86% and 19.01 to 20.14%, respectively. Fat level affected ( P<0.05) the weight losses, the chemical composition, the Gram −ve bacterial count, the lightness, the texture and the appearance of fermented sausages. Replacing 20% of pork backfat by olive oil affected ( P<0.05) the lightness and yellowness of sausages. Fat-reduced sausages without olive oil and low-fat sausages with olive oil had the highest score for odour and taste. However, the appearance of fat-reduced sausages was just acceptable while that of low-fat sausages was unacceptable, because the surface was intensively wrinkled and case hardening had developed. Further research is needed to improve the appearance of these sausages.

The acceptability of low fat sausages

Sausages from three major retail outlets producing their own label pork and reduced fat sausages were sampled over a period of four weeks. A branded product was also purchased from the stores. A trained sensory panel evaluated the sausages and found that, although the overall acceptability differed between retailers, one of the low fat products matched the equivalent standard product in terms of acceptability. The overall acceptability of the branded product was within the range of the own label standard products but below the most acceptable low fat product. It was concluded that, whilst the successful production of low fat sausages is possible, a wider range would be encouraged by the adoption of modified compositional standards.

The effect of cooking on the chemical composition of meat products with special reference to fat loss

The chemical composition and energy content of four product categories (sausages, restructured steaks, burgers and minced beef) were determined before and after cooking. Two cooking methods were used for each product category: sausages and burgers were fried or grilled, restructured steaks were grilled or cooked in an oven whilst mince was either boiled or lightly fried and then boiled to simulate domestic practice. Based on an initial 100 g of product and excluding the low fat products included in the survey, the average weights after cooking were 78, 73, 72 and 70 g for the sausages, restructured steaks, burgers and minced beef, respectively; average fat contents before and after cooking were 22 and 17 g for the sausages, 17 and 14 g for the restructured steaks, 25 and 16 g for the beef burgers, and 19 and 7 g for the mince; and average energy contents before and after cooking were 1215 and 1016 kJ for the sausages, 958 and 815 kJ for the restructured steaks, 1244 and 906 kJ for the burgers and 1009 and 544 kJ for the mince. For a given product type, cooking method was relatively unimportant; the cooking loss, fat loss and energy content of the cooked product being more dependent on product formulation and manufacturing process. The survey included a low fat sausage, a low fat burger, a lean mince and an extra lean mince with fat contents of 8.7, 7.5, 12.4 and 5.2%, respectively. Overall weight losses during cooking were similar to standard products, though low fat products lost proportionally more water and less fat. Frying resulted in a slight gain in fat content for the low fat sausages and burgers, based on an initial 100 g of product, though fried low fat products still had lower fat and energy contents than standard products. It is argued that national consumption figures should take into account the losses that occur during cooking to avoid overestimating fat and energy intake levels.

THERMO‐RHEOLOGICAL PROPERTIES AND COOKING YIELD OF SAUSAGE‐TYPE PRODUCTS AS AFFECTED BY LEVELS OF FAT AND ADDED‐WATER1

ABSTRACTConsumer interest in low fat foods has led meat processors to introduce low fat sausages with high added‐water. The purpose of this study was to use dynamic thermo‐rheological techniques to study the influence of fat and added water on sausage batters and cooking yields during heating. Twenty different sausage batters were formulated to 15–30% fat and 18–35% added water. Batters were heated in a thermal scanning rheometer from 20–80C in increments of IC/min. Rheograms of all batters showed a gradual decrease in storage modulus (G') as temperature increased from 20–59C. Significant changes in G' occurred in the range of 60–75C leading to the formation of rigid gel networks. Batters with levels of fat ≤ 24% and added water &gt; 24% or levels of fat and added water ≥ 27% showed increased cooking yields. Rheological measurements suggested that levels of added water influenced the G' of batters during heating and that high levels of added water could be used in sausage formulations without adversely affecting product yield.

Characteristics of High- and Low-Fat Bologna Sausages as Affected by Final Internal Cooking Temperature and Chilling Storage

A study was made of the effect that final internal processing temperature (63, 70 and 78°C) and chilling storage (2°C) exerted on the characteristics (cooking loss, purge loss, colour, Instron texture profile analysis) of high-fat (242 g kg -1 ) and low-fat (100 g kg -1 ) bologna sausage. High-fat sausages were harder and chewier than low-fat sausages. Lower fat contents were accompanied by a significant reduction in the cooking loss and purge loss. Binding properties were not affected (P > 0.05) by final internal cooking temperature. In general, Hunter colour parameter a values were higher in low-fat samples subjected to a high final internal cooking temperature than in those cooked up to only 63°C. High internal temperatures produced harder meat emulsions, an effect which was more pronounced in high-fat than in low-fat sausages. Cohesiveness and springiness of sausages was not affected (P > 0.05) by heat treatment. There were no major variations in textural parameters as a result of chilled storage.

Chopping temperature effects on the characteristics and chilled storage of low- and high-fat pork Bologna sausages

The object of this study was to investigate the influence of chilled storage (2 °C) and final chopping temperatures (8, 15 and 22 °C) on the characteristics and stability of Bologna sausage containing two levels of fat (8.5 and 23%). When the fat level was reduced there was a significant decrease in penetration force and an increase in both cooking and purge loss. A lower chopping temperature reduced purge loss during chilled storage. In general, an inverse relationship was found between chopping temperature and penetration force. Chopping temperature did not influence total aerobic count, but during chilled storage counts were significantly higher in the low fat sausages.

Frozen storage of Bologna sausages as a function of fat content and of levels of added starch and egg white

The effect of freezing and frozen storage on the water holding capacity (WHC) and texture of Bologna sausages formulated with varying fat levels ( 7.2, 12.8 and 20.1%) and with different proportions of added starch ( 0, 5 and 10%) and egg white (EW) ( 0, 1.5 and 3%) was studied. High-fat sausages exhibited better binding properties than low-fat sausages. Freezing and frozen storage caused binding properties to deteriorate, the more so the lower the fat content. In general, addition of starch caused an increase in penetration force and a decrease in elasticity of sausages, and also favoured freeze-thaw stability. Addition of egg white had no influence ( p > 0.05) on the WHC of Bologna sausages but did influence texture.

The Texas Breakfast Intervention Project: Introduction ‘of Ultrafine 100% White Whole Wheat to Improve Nutrient Profile

The purpose of this study was to increase implementation of the Dietary Guidelines (DG) in school breakfasts in Texas. Previous investigation indicated that the fiber content of school meals in Texas is low, and the fat content, especially saturated fat, is high. We conducted a visual plate waste analysis of over 800 children in Texas for eight weeks. During baseline (two weeks), children were fed standard breakfast items, averaging 0.5g fiber/day and 17.9g fat/day (7.4g saturated). For the following six weeks, products made with 100% ultrafine white whole wheat flour and lowfat items replaced regular fare (3.8g fiber/d, 9.5g fat/d, 3.5g saturated fat/d). Ultrafine white whole wheat flour is nutritionally similar to 100% red wheat, but bakes like refined white flour. Analysis of Variance was used to compare intake of experimental and baseline foods. Children accepted the grain-based experimental foods. Favorites were lowfat sausage, whole wheat cinnamon rolls, and whole wheat french toast. Children consumed lowfat milk as well as whole milk. Improving school breakfast by substituting familiar foods having superior nutritional profile is an effective technique for implementing the DG.

Sensory quality of low-fat sausages affected by fat substitutes

Fat substitutes have been introduced to low-fat meat products to make the texture more acceptable. This study focuses on the effect of the polysaccharides iota-carrageenan, xanthan and guar gum on the sensory quality of low-fat sausages. Low-fat sausages with these three fat-substitutes and sausages with regular fat content were analyzed by conventional sensory profiling. The data were analyzed by principal component analysis. The low-fat sausages with iota-carrageenan were most similar to the ordinary sausages. Low-fat sausages with guar gum and xanthan were similar to each other. Off-odours were perceived in the sausages with guar gum and xanthan, and these sausages were also less firm and less elastic than ordinary sausages and sausages with iota-carrageenan.

Effects of feeding diets containing different fat supplements to swine on the visual properties and storage stability of low-fat sausage

Sixty barrows and gilts were assigned to one of five dietary treatments consisting of a control diet of corn and soybean meal and four similar test diets that contained a 10% replacement of either animal fat, safflower oil, sunflower oil or canola oil, to determine the effects of high levels of oleic acid in the diet of swine on the storage stability of fresh pork sausage. Pork trim from each treatment was used to formulate sausage that contained two fat levels (25% and 35%), and two levels of added water (3% and 11%). Thiobarbituric acid values did not differ between the control, safflower oil or sunflower oil treatments and all treatments were acceptable after 10 weeks of storage. Microbial numbers increased with the level of added water and during time in storage (up to 3 weeks). Visual evaluation showed that the control was the most red and least discolored, while the canola oil treatment was the least red and the most discolored. Results from the present study suggest that a 105 replacement of a typical corn/soybean meal diet to swine with safflower or sunflower oil did not alter the storage-stability of fresh pork sausage.

Sensory quality of low-fat sausages affected by fat substitutes—A multivariate study

Sensory quality of low-fat sausages affected by fat substitutes—A multivariate study

Effects of various cellulose gums on the quality parameters of low-fat breakfast sausages

Composition, textural, viscoelastic, hydration, colour and sensory attributes of regular (26% fat) and low-fat (13%) pork breakfast sausages with and without cellulose gums (two types of carboxymethyl cellulose (CMC-I and -II) and microcrystalline cellulose (MCC-I and -II)) were investigated. The fat was replaced with water in low-fat products. In cooked low-fat sausages, the fat content decreased by 52–60%, and moisture content reduced by 61–65% of the initial values. The cooked high-fat products' composition showed an increase of about 6% fat and a decrease of 7% moisture due to cooking. MCC was more effective in retaining moisture compared to CMC. Product lightness was reduced due to both CMC and MCC. Gums have not affected fat content, water holding capacity, redness, yellowness, hardness, chewiness and gumminess. High-fat products were less elastic and easier to chew. Springiness was reduced by CMC-II and cohesiveness by other cellulose gums.

SELECTED SENSORY AND CHEMICAL ATTRIBUTES OF CHICKEN BREAKFAST SAUSAGES FROM DIFFERENT ANIMAL FAT SOURCES

Four breakfast sausage formulations were prepared to contain: (1) chicken lean + chicken fat (CF), (2) chicken lean + beef fat (BF), (3) chicken lean + pork fat (PF), or (4) chicken lean + high-oleic pork fat (PO). Formulations were targeted to contain 15% fat. Acceptability, tenderness, juiciness and flavor scores for each of the formulations as measured by a consumer taste panel (n = 71), were not different (P> 0.05). Sausages prepared with CF had a higher (P < 0.05) cooking loss and lower fat content than those prepared with BF. Thibarbituric acid (TBA) values for PO were significantly lower than those containing BF, PF, or CF. Sausage products containing chicken fat exhibited the highest TBA values during 9 days of storage at 4°C. These results suggested that chicken, beef, pork or high-oleic fats can be used as the fat source in low-fat chicken breakfast sausages without affecting the product acceptability.