You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology I1 Apr 2016MP86-06 COMBINATION THERAPY USING HUMAN ADIPOSE-DERIVED STEM CELLS ON THE CAVERNOUS NERVE AND LOW-ENERGY SHOCKWAVES ON THE CORPUS CAVERNOSUM IN A RAT MODEL OF POSTPROSTATECTOMY ERECTILE DYSFUNCTION Ohseong Kwon, Jin Bong Choi, Yong Hyun Park, Woong Jin Bae, Hyuk Jin Cho, U-Syn Ha, Sung Hoo Hong, Se Woong Kim, and Ji Youl Lee Ohseong KwonOhseong Kwon More articles by this author , Jin Bong ChoiJin Bong Choi More articles by this author , Yong Hyun ParkYong Hyun Park More articles by this author , Woong Jin BaeWoong Jin Bae More articles by this author , Hyuk Jin ChoHyuk Jin Cho More articles by this author , U-Syn HaU-Syn Ha More articles by this author , Sung Hoo HongSung Hoo Hong More articles by this author , Se Woong KimSe Woong Kim More articles by this author , and Ji Youl LeeJi Youl Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2314AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We investigated combined therapeutic efficacy of human adipose-derived stem cells (h-ADSCs) application on injured cavernous nerve and low-energy shockwave therapy (SWT) on the corpus cavernosum in a rat model of post-prostatectomy erectile dysfunction. METHODS Rats were randomly divided into five groups: Control, BCNI (bilateral cavernous nerve injury), ADSC (BCNI group with h-ADSCs on the cavernous nerve), SWT (BCNI group with low-energy SWT on the corpus cavernosum), and ADSC/SWT (BCNI group with a combination of h-ADSCs and low-energy SWT). After four weeks, erectile function was assessed using intracavernosal pressure (ICP). The cavernous nerves and penile tissue were evaluated through immunostaining, western blotting and a cyclic guanosine monophosphate (cGMP) assay. RESULTS ADSC/SWT significantly improved ICP compared to the other experimental group. ADSC had significantly increased ß-III tubulin expression of cavernous nerve, and SWT had markedly enhanced vascular endothelial growth factor (VEGF) expression in corpus cavernosum. The ADSC/SWT group had a significantly increased in alpha smooth muscle content (P <.05), neural nitric oxide synthase (nNOS) of the dorsal penile nerve (P <.05), endothelial nitric oxide synthase (eNOS) protein expression (P <.05), and cGMP level (P <.05) compared to ADSC or SWT alone group. In addition, ADSC/SWT reduces the apoptotic index in corpus cavernosum. CONCLUSIONS In this study, h-ADSCs showed effect on recovery of injured cavernous nerve and low-energy SWT improved angiogenesis in the corpus cavernosum. The h-ADSCs combined with low energy SWT showed beneficial effect on the recovery of erectile function in a rat model of post-prostatectomy erectile dysfunction. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1108 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Ohseong Kwon More articles by this author Jin Bong Choi More articles by this author Yong Hyun Park More articles by this author Woong Jin Bae More articles by this author Hyuk Jin Cho More articles by this author U-Syn Ha More articles by this author Sung Hoo Hong More articles by this author Se Woong Kim More articles by this author Ji Youl Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...