AbstractThere is no information available on the influence of nitrogen (N) fertilization on autumn‐accumulated forage under Central European conditions. In this context, the metabolizable energy and the degree of fungal infection have never been examined before. In this study, the effects of the amount of N fertilizer (0, 50, 100 and 150 kg N ha−1), the date of N application (July and August) and the date of winter harvest (December, January and February) on the quality and dry matter (DM) yield of Festuca arundinacea stands in winter were investigated. DM yield, the metabolizable energy and digestibility of organic matter (= in vitro rumen fermentation technique), crude protein, ADL (acid detergent lignin), and for the first time ergosterol were determined. Disregarding the 0 variant, no interactions of any relevance were present. DM yield, crude protein and values of ADL increased with increasing amounts of N, but the concentration of energy decreased. The late N application resulted mostly in lower DM yields but kept the forage physiologically younger, which brought about higher levels of metabolizable energy and lower concentrations of ADL. With later date of harvest, losses of mass and quality occurred. The degree of fungal infection rose as the amount of N fertilizer increased. Three years of investigation under different weather conditions, including interactions with the effects of N fertilization, provide a good base for generalization.