Low-dose Settings Research Articles

Low-dose cytarabine sets the bar for treatment of elderly with AML

Low-dose cytarabine sets the bar for treatment of elderly with AML

Low-dose computed tomography: A solution for in vivo medical imaging and accurate patient-specific 3D bone modeling?

The number of in vivo clinical biomedical experiments based on computed tomography is increasing. International radiation-protection bodies are promoting the use of low-dose computed tomography to reduce radiation absorption by the subject undergoing imaging. On the other hand no data exist in the literature to quantify whether or not low-dose computed tomography would lead to a decrease of result quality when used for three-dimensional bone modeling and related measurements. This paper aimed at finding a consensus between minimal X-ray radiation of the subject, and satisfactory image data quality, especially for accurate three-dimensional bone modeling. Several standard computed tomography and low-dose computed tomography sequences were analyzed in three tests and statistically compared. Absence of significant difference between standard and low-dose computed sequences indicated that the low-dose setting would not produce less accurate three-dimensional models, while it decreased the effective X-ray dose up to 90% compared to standard settings. Low-dose computed tomography seems suitable for accurate three-dimensional bone modeling, while the related effective X-ray radiation is low. Such setting is therefore advised for any in vivo medical imaging aiming to collect bone data.

360. Disturbance of Airway Epithelium Is Reduced, Whilst Lentivirus-Mediated Gene Transfer Is Maintained, after Low Dose Lysophosphatidylcholine Pre-Treatment In Vivo

Introduction: In developing an effective gene therapy for CF lung disease we have shown that pre-treatment of mouse nasal respiratory epithelium with the detergent lysophosphatidylcholine (LPC) enhances lentivirus (LV)-mediated gene transfer in vivo. Initial studies showed significant gene transfer, however areas of cell damage were also evident. This study sought to find lower dose settings that achieved similar gene transfer, without histologically apparent disruption of the epithelium.

Tumor necrosis factor-alpha augmented tumor response in B16BL6 melanoma-bearing mice treated with stealth liposomal doxorubicin (Doxil) correlates with altered Doxil pharmacokinetics.

The application of tumor necrosis factor-alpha (TNF) for the treatment of solid tumors is limited by its severe, life-threatening, toxicity. Therefore, only low dosages of this cytokine can be applied systemically, which results in poor tumor response. It has been demonstrated previously that administration of high-dose TNF in a so-called isolated perfusion system markedly improved tumor response when combined with chemotherapy. It appeared that TNF had a major impact specifically on the tumor-associated vasculature. At these high concentrations, endothelial cell death is induced by TNF, resulting in complete collapse of the tumor vascular bed. Strikingly, this effect alone is not enough to induce a tumor response, but addition of a chemotherapeutic drug is mandatory to obtain an anti-tumor effect. We showed that TNF has no anti-tumor effect by itself but augmented drug accumulation mainly in the tumor, most likely by enhancing vascular leakage. It seems that enhanced vascular leakage, but not endothelial cell death, explains the interaction between TNF and the co-administered drug. We hypothesized that in a low-dose setting TNF could induce tumor accumulation of chemotherapeutic drugs and consequently improve tumor response. We demonstrate that free TNF has a strong effect on the pharmacokinetics of co-administered Doxil in B16BL6 melanoma-bearing mice, resulting in strongly augmented drug accumulation in the tumor and improved tumor response. Co-injection of Stealth liposomal TNF with Doxil resulted in comparable or less pronounced tumor responses as compared to free TNF. These results imply that systemic application of clinically tolerable doses of TNF may improve drug distribution and tumor response and could be useful in a number of anti-cancer therapies.

Comparison of Indirect CsI/a:Si and Direct a: Se Digital Radiography. An assessment of contrast and detail visualization

To assess and quantify the image quality at two dose levels for an amorphous Silicon (a:Si) Cesium Iodide (CsI) flat panel system compared with a direct amorphous Selenium (a:Se) digital radiography system. A contrast detectability test was performed employing the CDRAD-phantom at mAs-values leading to approximately equal phantom entrance doses of 41.4, 57.9, 75.1 and 120.8 micro Gy for the a:Se and 39.9, 58.4, 75.6 and 117.9 micro Gy for the CsI system. Images were presented to 4 independent observers. For quantitative comparison, the image quality figure (IQF) was calculated. Statistical analysis was performed using Pearson's correlation and the Wilcoxon test. A ROC-analysis was performed employing the TRG-phantom in a high- and a low-dose setting leading to entrance doses of 126.2 and 35 micro Gy for the direct, and 125.9 and 34.4 micro Gy for the indirect system. Statistical significance was evaluated using the Wilcoxon test. The flat panel a:Si digital system provided superior results compared with the a:Se drum digital system with respect to low-dose settings for CDRAD-phantom and ROC-analysis, ensuring a better image quality with respect to contrast and detail detectability. Higher-dose settings provided similar results for both systems. Image quality of a:Si flat panel digital radiography proved to be superior to a:Se drum digital radiography using low-dose settings. If the primary target is dose reduction indirect flat panel technology should be used.

Low-dose CT and inflammatory disease of the paranasal sinuses

Computed tomography (CT) is the gold standard for exact delineation of inflammatory sinus disease, especially before endoscopic surgical treatment, and in cases of postoperative recurrences. In routine CT studies, the radiation dose to the patient is not negligible. Therefore, the authors evaluated prospectively the CT scans of 44 patients with inflammatory disease of the paranasal sinuses, to define the imaging ability of low-dose CT (i.e. 60 mA-3 s, 30 mA-3 s, and 30 mA-2 s), comparatively with the standard mAs settings (130 mA-3 s). In all cases, the exact extent of the disease was correctly assessed on each of the low-dose settings, with no false negative study. The increasing graininess of low mAs sections did not induce errors of interpretation, despite a less pleasant appearance to the eyes. In cases of extensive sinus disease, the thickness and integrity of the ethmoid septa were sometimes more difficult to evaluate on low-dose CT sections. The authors recommend the use of low mAs settings in the evaluation of inflammatory disease of the sinuses, complemented, if necessary, in cases of extensive abnormalities, by one or two sections obtained with standard mAs settings, focused on questionably abnormal bone septa.