Low-cost Test Research Articles

Respiratory Syncytial Virus Infection in Children with Bronchiolitis

Background: Respiratory Syncytial Virus (RSV) is the most common organism causing bronchiolitis. Bronchiolitis is almost always diagnosed clinically. As a result, the availability of bedside sensitive rapid RSV antigen tests is critical for timely RSV acute respiratory infection diagnosis. Methods and materials: This study was conducted to detect RSV antigen positivity and the sociodemographic profile of children with RSV antigen positive bronchiolitis. From October 2022 to March 2023, this observational study was conducted in the pediatrics department of Chattogram Medical College Hospital. Ninety-two nasopharyngeal swabs from children younger than two years, were tested for RSV antigen. The mean and standard deviation were used to express the demographic data. Chi-square analysis was used to examine differences in group proportions. Results: RSV antigen positivity was detected in 45.7% of the 92 cases, with a mean age of 4.18±3.08 months. Severe bronchiolitis cases were more common (22.8%) among antigen positive cases and had the longest hospital stay. During the months of November (42.4%) and December (29.3%), RSV infection levels peaked. This study describes that during the seasonal epidemics, RSV was the main concern, as the number of positive cases decreased in the months that followed. The signs and symptoms of RSV positive and negative cases were not significantly different. Conclusion: A simple, low-cost and bedside RSV test can be considered as a valuable diagnostic tool in a low resource setting. J Bangladesh Coll Phys Surg 2024; 42: 368-378

Newborn Genomic Sequencing Needs Confirmation but Not Repeating

Newborn screening (NBS) has been one of the big innovations in public health. It has grown over the decades, especially with the introduction of tandem mass spectrometry. However, it is likely to expand significantly in the coming decades with the introduction of genomic testing. Traditionally, in NBS, there has been a pattern of repeat testing for confirmation and follow-up diagnostic testing. This follow-up is critical as NBS is a screening program. This pathway is appropriate for low-cost tests, but if public health authorities are going to invest in high-cost screening such as whole-genome sequencing, they are likely to baulk at repeating these expensive tests in a diagnostic setting. Our study investigates whether screening-grade data from NBS can be transitioned into diagnostic-grade data using a panel of single-nucleotide variants (SNVs) on a diagnostic specimen. These SNVs could be used to link the diagnostic specimen with all of the provenance requirements associated with routine pathology and the NBS genomic data. This strategy has large cost benefits and opens up the rapid use of NBS genomic data should a child present in an acute care setting and a genetic diagnosis is suspected. This approach will greatly speed up the confirmation of positive NBS results and reduce family anxiety due to delayed diagnostic testing.

Instrument for Evaluation and Training of Decision Making in Dual Tasks in Soccer: Validation and Application

Training in team sports such as soccer requires advanced technical and tactical skills for effective decision-making, particularly when executing a shot. This study validates an innovative instrument, a training platform (TP), designed to measure and enhance decision-making in dual-task scenarios. The TP aims to improve visual–motor reactions in multitask environments that simulate real game conditions. Equipped with an LED panel, main circuitry, ball sensor, and targets, the TP challenges players to kick the ball in response to the illumination of the final LED array on the panel while hitting a designated target. The study evaluated three parameters: reaction time (RT), ball speed (BS) and accuracy. To validate the TP against a gold standard (GS), we conducted correlation analyses. The results exhibited very strong correlations for both RT (r = 0.997) and BS (r = 0.994). The mean differences between TP and GS measurements were 13 ± 15 ms for RT and 0.1 ± 0.5 km/h for BS. Bland–Altman plots revealed trend lines obtained by a simple linear regression of r = −0.507, p = 0.307 for RT and r = 0.134, p = 0.077 for BS. The TP effectively simulates game scenarios, offering advantages such as low-cost components, installation flexibility, test variability, instant feedback, and integration of physical and cognitive components of sports performance.

Validation of the Diabetomics CovAB SARS-CoV-2 antibody test in children: comparison with serology.

Recently a study determining the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies in unvaccinated children in NJ (Katic et al. 2023. Pediatric Academic Societies Meeting; Washington, D.C. https://2023.pas-meeting.org/searchbyposterbucket.asp?bm=Public+Health+%26+Prevention&t=Public+Health+%26+Prevention&pfp=Track) was conducted using a gum swab method for antibody detection. The Diabetomics CovAB test, which qualitatively identifies antibodies to SARS-CoV-2 spike protein, is a point-of-care, low-cost test, that is easy to administer in children. While this test provides sensitive and specific results in adults [US Food and Drug Administration (FDA). 2022. Center for devices and radiological health. EUA authorized serology test performance. Available from: https://www.fda.gov/medical-devices/covid-19-emergency-use-authorizations-medical-devices/eua-authorized-serology-test-performance], data on its accuracy in children is lacking. As a follow-up to the above-mentioned study, we compared the results of the gum swab test to a serologic antibody test. We found that the gum swab test was inferior to serology but was fairly sensitive and specific with a high positive predictive value. While the test is not ideal for diagnostic purposes in children it can be a valuable tool for public health officials and pediatricians to understand the extent of past health interventions.

Air Leakages at Microvalves: Pressure Decay Measurements and Extended Continuum Modelling of Knudsen Flows

To improve the performance of valves in relation to the leakage rate, a comprehensive evaluation of the valve characteristics and behavior during pressure exposure is important. Often, these low gas flow rates below 0.1 cm3/min cannot be accurately measured with conventional flow sensors. This paper presents a small and low-cost test rig for measuring gas leakage rates accurately, even far below 0.1 cm3/min, with the pressure decay method. These leakage flows are substantiated with a flow model, where we demonstrate the feasibility of modeling those gas flows with an extended Navier–Stokes framework to obtain more accurate theoretical predictions. As expected, the comparison to the experimental results proves that the classical Navier–Stokes system is unsuitable for modeling Knudsen flows. Hence, self-diffusion of gas, a wall-slip boundary condition, and an effective mean free path model were introduced in a physically evident manner. In terms of the calculated mass flow, while self-diffusion and slip boundary conditions explain deviations from the classical Navier–Stokes equation for Knudsen numbers already smaller than 1, the effective mean free path model has an effect, especially when Kn > 1. For simplified conditions, an analytical solution was presented and compared to the results of an OpenFOAM CFD-solver for flow rates through more complex gap-flow geometries of the flap valve. Hereby, acceptable deviations between 10% and 20% were observed. A comparison with measurement results was carried out. The reproducibility of the measurement method was verified by comparing multiple measurements of one silicon microvalve sample to a state-of-the-art flow sensor. Three geometrically similar passive silicon microvalves were measured with air overpressure decreasing from 15 kPa relative to atmospheric pressure. Maximum gas volume flowing in a blocking direction of 1–26 µL/min with high reproducibility and marginal noise were observed.

Imaging research on damage localization of concrete structure based on Lamb wave and Bayesian fusion algorithm

Concrete structures are critical materials in the field of industrial construction and are susceptible to invisible damage under the influence of a variety of factors. The method of increasing the number of sensors is usually used to obtain high-precision damage detection, but it increases the experimental cost and signal processing time, so it is especially important to perform high-precision and low-cost non-destructive testing for it. The article uses Bayes to combine the elliptical trajectory method with a probabilistic damage imaging algorithm, and utilizes the algorithm to achieve higher accuracy damage localization imaging results with a smaller number of sensors. The wavelet transform is used to calculate the time of flight to obtain the localization results of the elliptical trajectory method, the signal damage factor is calculated based on the amplitude information to obtain the localization results of the probabilistic damage imaging, the probability distribution of the damage location is obtained using Bayesian, and the damage localization results are obtained by solving the problem through the Markov chain Monte Carlo method. A dense sensor array based and probabilistic imaging algorithm is also used to localize the damage to the material and the results are compared with the results of the Bayesian fusion algorithm. The results show that the Bayesian fusion algorithm can obtain higher accuracy of damage localization with a significant reduction in the number of sensors, and at the same time greatly reduces the number of signals, simplifies the experimental process, and has high practical value.

Complete blood count as a biomarker for preeclampsia with severe features diagnosis: a machine learning approach

ObjectiveThis study introduces the complete blood count (CBC), a standard prenatal screening test, as a biomarker for diagnosing preeclampsia with severe features (sPE), employing machine learning models.MethodsWe used a boosting machine learning model fed with synthetic data generated through a new methodology called DAS (Data Augmentation and Smoothing). Using data from a Brazilian study including 132 pregnant women, we generated 3,552 synthetic samples for model training. To improve interpretability, we also provided a ridge regression model.ResultsOur boosting model obtained an AUROC of 0.90±0.10, sensitivity of 0.95, and specificity of 0.79 to differentiate sPE and non-PE pregnant women, using CBC parameters of neutrophils count, mean corpuscular hemoglobin (MCH), and the aggregate index of systemic inflammation (AISI). In addition, we provided a ridge regression equation using the same three CBC parameters, which is fully interpretable and achieved an AUROC of 0.79±0.10 to differentiate the both groups. Moreover, we also showed that a monocyte count lower than 490/mm3\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$490 /mm^{3}$$\\end{document} yielded a sensitivity of 0.71 and specificity of 0.72.ConclusionOur study showed that ML-powered CBC could be used as a biomarker for sPE diagnosis support. In addition, we showed that a low monocyte count alone could be an indicator of sPE.SignificanceAlthough preeclampsia has been extensively studied, no laboratory biomarker with favorable cost-effectiveness has been proposed. Using artificial intelligence, we proposed to use the CBC, a low-cost, fast, and well-spread blood test, as a biomarker for sPE.

PSIII-3 Evaluation of low-cost cow-side tests in developing a selective dry cow therapy program

PSIII-3 Evaluation of low-cost cow-side tests in developing a selective dry cow therapy program

Comparison of post-impact residual strength ratio between hybrid filament-wound cylinder and hybrid plate specimen

The filament-wound (FW) cylinders possess numerous benefits as a high-pressure storage device but are susceptible to impact loads. The presence of post-impact residual strength in an operating FW cylinder has the potential to accelerate the onset of failure. Ensuring the structural integrity and safety of the equipment requires thorough impact testing, which can be expensive and time-consuming, resulting in limited use. This article presents an innovative and cost-effective approach for conducting low-velocity impact testing on a hybrid Al/CFRP filament-wound cylinder by utilizing a simple hybrid Al/CFRP plate specimen. The main focus is to conduct low-velocity impact testing on both types of specimens, followed by post-damage testing that includes tensile tests on the hybrid plate specimen and burst tests on the FW cylinder. The results of laboratory experiments were compared to explicit and implicit numerical models conducted in a broad spectrum of impact energy range and found to be consistent. Using a parameter known as the post-impact residual strength ratio, it is possible to determine the expected impact loading conditions on the hybrid plate specimen that are equivalent to those experienced by the FW-cylinder. This low-cost impact testing method offers a practical and feasible solution to ensure the dependability and safety of FW cylinders throughout their operational lifespan.

Validation of a Low-Cost Manometer to Assess of Tongue, Lip, Cheek, and Respiratory Strength: A Laboratory-Based Study.

The objective of this study was to characterize the level of agreement between three manometers: (1) Iowa Oral Performance Instrument (IOPI)-the reference standard for tongue, lip, and cheek strength assessments; (2) MicroRPM Respiratory Pressure Meter (MicroRPM)-the reference standard for respiratory strength assessments; and (3) Digital Pressure Manometer (DPM)-an alternative, low-cost pressure testing manometer. Manual pressures were simultaneously applied to the IOPI and DPM, and to the MicroRPM and DPM, within a controlled laboratory setting. Agreement in pressure readings were analyzed using descriptive statistics, Lin's concordance correlation, and Bland-Altman Plots. Agreement was interpreted as "poor" if ρc < 0.90, "moderate" if ρc = 0.90 - < 0.95, "substantial" if ρc = 0.95 - < 0.99, and "excellent" if ρc ≥ 0.99. Differences in pressure readings between the DPM and clinical reference standards were consistently present yet highly predictable. There was a median absolute difference of 2.0-3.9 kPa between the IOPI and DPM, and 4.5-9.8 cm H2O between the MicroRPM and DPM. Lin's concordance revealed "substantial" agreement between the IOPI and DPM (ρc = 0.98) and the MicroRPM and DPM (ρc = 0.99). The DPM revealed higher pressure readings when compared to the IOPI and MicroRPM. However, differences in pressure readings were relatively small, highly predictable, and yielded substantial overall agreement. These findings suggest the DPM may be a valid, lower-cost alternative for objective assessments of tongue, lip, cheek, and respiratory muscle strength. Future research should expand on the present findings in clinical patient populations. NA Laryngoscope, 2024.

An Iterative Approach to Usability Evaluation: A Workplace Wellness Mobile Application Example.

Without a gold standard for mobile app usability testing, this paper outlines a case study of how a not-for-profit organization tested the usability of a public-facing mobile app designed to promote the health and wellbeing of workers in Canada by offering accessible resources conveniently through one's mobile phone. Although the app was developed by a third-party vendor, usability testing and refinement was left to the organization due to budgetary and time constraints. The app underwent three rounds of iterative usability testing. The first phase consisted of observing participants using the app followed by an interview. The second and third phases involved participants following task-based instructions and answering survey questions. All phases uncovered issues which helped to improve and refine the usability of the app before the launch to the public. This work offers a case study example of how low-cost in-house usability testing could be employed.

Diagnostic Accuracy of Low-Cost Blood Test Inflammatory Markers in Severe Ulcerative Colitis

Diagnostic Accuracy of Low-Cost Blood Test Inflammatory Markers in Severe Ulcerative Colitis

An efficient and quantitative approach for fracture toughness measurement of Micron-Thick hard coatings based on crack spacing

Micron-thick hard coatings find widespread applications, with fracture toughness being a crucial property that ensures their usability. However, measuring the fracture toughness of hard coatings is time-consuming and costly due to the susceptibility to cracking and small sample size. Thus, developing a simple, effective and precise measurement method is necessary. This work presents an analytical model that calculates KC from crack spacing induced by uniaxial tension. By measuring the variation of crack spacing, this model determines the fracture toughness from the energy difference during the coating fracture, and residual stress can also be estimated. This simple method overcomes the limitations of uniaxial tension methods, does not require any in-situ observations, and offers low-cost and user-friendly testing. The KC of TiN coatings with 2 μm thickness were tested and found to match the micro-cantilever beam test results from the literature. This model used data from other literature to calculate the toughness of diamond and a-C:H thin coatings, showing its generality. Additionally, our model’s estimation of residual stress in coatings, as reported in the literature, is accurate to within 0.1 ∼ 1 GPa.

D-dimer/high sensitive troponin I ratio is useful in predicting in-hospital mortality in pulmonary embolism patients.

Pulmonary embolism requires careful differential diagnosis as it is associated with a wide range of symptoms that may suggest different diseases such as chest pain, shortness of breath and syncope. Since the disease can be fatal, especially in cases where right ventricular failure and hemodynamic instability develop, prognostic markers are great importance in terms of monitoring the patient during the treatment process. We aimed in our study to compare the relationship between the ratio of D-dimer and High Sensitive Troponin T (HsTnT) values ​​with short-term mortality and to compare this relationship with Pulmonary Embolism Severity Index (PESI) scoring. Our study was conducted with patients who applied to the emergency department of our hospital between 01/01/2022 and 01/01/2023 and were definitively diagnosed withPulmonary thromboembolismafter their evaluation. The success of D-dimer/HsTroponin, D-dimer/CK-MB and troponin/D-dimer indices calculated from the laboratory test results of the cases in predicting mortality was examined, and a comparison was made with the success of the PESI score in predicting mortality. Among these indices, D-dimer/CK-MB was found to be the most successful index in predicting 7-day mortality (AUC: 0.734; 95% CI: 0.653-0.815; p < 0.001). Additionally, the D-dimer/HsTroponin ratio was found to be statistically significant as a successful index in predicting 7-day mortality (AUC: 0.697; 95% CI: 0.621-0.774; p < 0.001). FD-dimer/HsTroponin ratio, which is a powerful, fast, low-cost, easy and simple test, can be used especially in emergency services instead of the PESI score as a mortality marker in pulmonary embolism, which has a high mortality rate.

Development of a Diagnostic Microfluidic Chip for SARS-CoV-2 Detection in Saliva and Nasopharyngeal Samples.

The novel coronavirus SARS-CoV-2 was first isolated in late 2019; it has spread to all continents, infected over 700 million people, and caused over 7 million deaths worldwide to date. The high transmissibility of the virus and the emergence of novel strains with altered pathogenicity and potential resistance to therapeutics and vaccines are major challenges in the study and treatment of the virus. Ongoing screening efforts aim to identify new cases to monitor the spread of the virus and help determine the danger connected to the emergence of new variants. Given its sensitivity and specificity, nucleic acid amplification tests (NAATs) such as RT-qPCR are the gold standard for SARS-CoV-2 detection. However, due to high costs, complexity, and unavailability in low-resource and point-of-care (POC) settings, the available RT-qPCR assays cannot match global testing demands. An alternative NAAT, RT-LAMP-based SARS-CoV-2 detection offers scalable, low-cost, and rapid testing capabilities. We have developed an automated RT-LAMP-based microfluidic chip that combines the RNA isolation, purification, and amplification steps on the same device and enables the visual detection of SARS-CoV-2 within 40 min from saliva and nasopharyngeal samples. The entire assay is executed inside a uniquely designed, inexpensive disposable microfluidic chip, where assay components and reagents have been optimized to provide precise and qualitative results and can be effectively deployed in POC settings. Furthermore, this technology could be easily adapted for other novel emerging viruses.

ROS1 fusions in resected stage I-III adenocarcinoma: Results from the European Thoracic Oncology Platform Lungscape project

BackgroundROS1 fusion is a relatively low prevalence (0.6–2.0%) but targetable driver in lung adenocarcinoma (LUAD). Robust and low-cost tests, such as immunohistochemistry (IHC), are desirable to screen for patients potentially harboring this fusion. The aim was to investigate the prevalence of ROS1 fusions in a clinically annotated European stage I-III LUAD cohort using IHC screening with the in vitro diagnostics (IVD)-marked clone SP384, followed by confirmatory molecular analysis in pre-defined subsets. MethodsResected LUADs constructed in tissue microarrays, were immunostained for ROS1 expression using SP384 clone in a ready-to-use kit and Ventana immunostainers. After external quality control, analysis was performed by trained pathologists. Staining intensity of at least 2+ (any percentage of tumor cells) was considered IHC positive (ROS1 IHC + ). Subsequently, ROS1 IHC + cases were 1:1:1 matched with IHC0 and IHC1 + cases and subjected to orthogonal ROS1 FISH and RNA-based testing. ResultsThe prevalence of positive ROS1 expression (ROS1 IHC + ), defined as IHC 2+/3+, was 4 % (35 of 866 LUADs). Twenty-eight ROS1 IHC + cases were analyzed by FISH/RNA-based testing, with only two harboring a confirmed ROS1 gene fusion, corresponding to a lower limit for the prevalence of ROS1 gene fusion of 0.23 %. They represent a 7 % probability of identifying a fusion among ROS1 IHC + cases. Both confirmed cases were among the only four with sufficient material and H-score ≥ 200, leading to a 50 % probability of identifying a ROS1 gene fusion in cases with an H-score considered strongly positive. All matched ROS1 IHC- (IHC0 and IHC1 + ) cases were also found negative by FISH/RNA-based testing, leading to a 100 % probability of lack of ROS1 fusion for ROS1 IHC- cases. ConclusionsThe prevalence of ROS1 fusion in an LUAD stage I-III European cohort was relatively low. ROS1 IHC using SP384 clone is useful for exclusion of ROS1 gene fusion negative cases.

UK Museums and Heritage Sustainable Packing Group: Exploring Sustainable Strategies for Soft Wrapping Paintings

ABSTRACT This paper explores methods to improve the sustainability of packing artworks, focusing on soft wrapping of framed paintings. In 2022, the UK Museums and Heritage Sustainable Packing Group was formed. The research described in this paper focuses on one aspect of the group’s activity: seeking to find materials and methods which reduce environmental burdens and comply with conservation standards for soft wrapping two-dimensional artworks for short journeys between display, storage locations and conservators’ studios. The project investigated the physical qualities of recycled content polyethylene (PE), bioplastics and paper-based alternatives, along with common barrier materials such as virgin PE sheet and Bubble Wrap®, to form comparative datasets. Each material’s environmental impact, potential for reuse, interaction with artworks and ability to maintain adequate buffering against environmental changes during transport was researched. Alternative methods were also explored to reduce the quantity of sealing tape and promote its reversibility and reuse. The environmental impacts of the materials were assessed by sustainability specialists at the University of Manchester’s Sustainable Materials Innovation Hub. Reusable and recycled content films offered the most promising alternatives to the PE wrapping most used at present. The use of a tying method, eliminating the need for adhesive tape, also showed potential for further investigation. The low-cost testing methodology should be refined to better control the variables and allow for more repeatable testing, but these preliminary results will help to refine future research directions.

Centralized or Onsite Testing? Examining the Costs of Water Quality Monitoring in Rural Africa.

Rural water systems in Africa have room to improve water quality monitoring. However, the most cost-effective approach for microbial water testing remains uncertain. This study compared the cost per E. coli test (membrane filtration) of four approaches representing different levels of centralization: (i) one centralized laboratory serving all water systems, (ii) a mobile laboratory serving all systems, (iii) multiple semi-centralized laboratories serving clusters of systems, and (iv) decentralized analysis at each system. We employed Monte Carlo analyses to model the costs of these approaches in three real-world contexts in Ghana and Uganda and in hypothetical simulations capturing various conditions across rural Africa. Centralized testing was the lowest cost in two real-world settings and the widest variety of simulations, especially those with water systems close to a central laboratory (<36 km). Semi-centralized testing was the lowest cost in one real-world setting and in simulations with clustered water systems and intermediate sampling frequencies (1-2 monthly samples per system). The mobile lab was the lowest cost in the fewest simulations, requiring few systems and infrequent sampling. Decentralized testing was cost-effective for remote systems and frequent sampling, but only if sampling did not require a dedicated vehicle. Alternative low-cost testing methods could make decentralized testing more competitive.

Overall Summery of Gulf Country Employees from Bangladesh, Preliminary Evaluation for Abnormal Kidney Liver and Virally Infected Individuals Using Low-cost Tests Like CBC Parameters

Hepatitis B Viruses antigen (HBsAg), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV) and Syphilis is a major public concern because of shared route of transmission. Alanine transaminase (ALT) and Aspartate Aminotransferase (AST) and Bilirubin is measured for abnormal liver function test and Abnormal Kidney function is measured by creatinine. The complete blood count (CBC) is the most common examination used to monitor overall health in clinical practice as well as cheap in Bangladesh. Radiological Image could have cheap but HBsAg, HCV, HIV and Syphilis test is cost effective. The main aim to this study is to determine the viral positive, abnormal Kidney, Liver function patient’s detection by doing cheap CBC test parameters. In this study, we have calculated normal patients CBC parameter and same HBsAg, HCV and syphilis patients, abnormal kidney and liver functions patients CBC parameters have been assembled. We also find out the relation with radiological image of chest X-ray for viral positive patients. The result of the study indicated that all CBC parameter of positive HBsAg, HCV and syphilis, abnormal kidney and liver patients approximately same. But only Mean Platelet Volume (MPV) and Platelet number have significant value. Abnormal Kidney and liver patients have high MPV 11.94x10^3 and HCV, HBsAg positive patients 9.9x10^3 whereas normal patients value is 9.7x10^3. This study proves that there is significant effect for MPV value with normal and viral infected patients. This finding could have used early detection for overseas health checkup.

Exploring the influence of washing activities on the transfer and persistence of fibres in forensic science

In forensic science, a robust and sound interpretation and evaluation of transferred fibre evidence requires an understanding of the principles and mechanisms that underpin fibre transfer, yet existing research lacks consistency and repeatability. This study investigates the impact of washing activities on both the release of fibres into wastewater and the transfer of constituent fibres from donor garments to receiver swatches. Using a low-cost friction tester and automated data collection through photography and ImageJ image processing software, controlled conditions were maintained for repeated experiments. Results indicated significant fibre release during wash cycles, with load size and donor garment history playing crucial roles. The donor garments subjected to repetitive washes exhibit a progressive decrease in the number of fibres transferred, independently of the load size. This study underscores the importance of considering a garment's washing history in forensic science contexts, but also for consistency in the way that data are collected.