Lower Body Condition Research Articles

Welfare assessment at a Spanish Army Equine Breeding Centre

The study aimed to assess the effect of two management housing system on breeding horse welfare. An adapted version of the assessment protocol for horses was used for that purposed to this study, with the focus on the animal-based measures section. This includes individual animal scoring of good feeding, good health and appropriate behaviour. The study was conducted in a professional Equine Breeding Centre with a representative set of housing and management systems and practices used for breeding purposes in Spain. A total of 49 stallions stabled individually and 43 broodmares living loose housing in groups were subject for individual scoring. A descriptive statistic for each welfare indicator was calculated. Pearson’s Chi-square test or Fisher’s test were calculated between the different welfare measures and the housing system. Odds ratios were computed to quantify the association of prevalence of welfare issues with husbandry and management practices according to the type of housing. The stallions were almost two and a half times more at risk than mares to exhibit acceptation to the chin contact behavioural test. Broodmares were prone to present significant lower body condition scores (BCS). The prevalence of rubbed and broken hairs in mane and tail (OR = 4.413), gait abnormalities (OR = 5.429), skin lesions in upper and lower legs regions (OR = 2.691) was higher in stabled animals. In contrast, broodmares presented more often with general skin lesions, specifically in withers-back-barrel-girth (OR = 0.349), hindquarters (OR = 0.016) and skin irritation in lower legs (OR = 0.287). The risk factor analysis applying a multivariable logistic regression model disclosed the way that the group housing freedom system management predicted significantly the prevalence of three animal based indicators deviations: one behavioural indicator (fear to the novel object test), one feeding indicator (lower BCS) and one health indicator (harm in lower legs). Highlights The the effect of management housing system on breeding horse welfare could be evaluated using a protocol with special focus on individual animal scoring of feeding, health and behaviour. The major welfare problems identified in the horses housed in individual boxes were associated with confinement and could be minimised with better management of the access to free exercise. The source of fear and aggression in horses living in pastures can greatly improve with intensive and correct human handling and providing sufficient feeding resources.

Biological parameters of the High-Antarctic icefish, Cryodraco antarcticus (Channichthyidae) from the South Shetland Islands

Despite their wide distribution around the Antarctic continent, the life strategies of the long-fingered icefish Cryodraco antarcticus have been rarely investigated and are not well understood. The aim of this paper was to provide more insights on the demographic characteristics of the population living off the South Shetland Islands, focusing attention on key biological traits such as age and growth and the reproductive cycle. Individual age and reproductive status were assessed through the microstructural analyses of sagittal otoliths and histological analysis of gonads, respectively. The sex-balanced sampled population consisted of juvenile and adult fishes covering a wide size range. Both sexes had positive allometric growth, although males exhibited smaller maximum size and lower body condition than females. Fish longevity was comparable between sexes, being 16 and 18 years in males and females, respectively. Applying the von Bertalanffy growth model to length-at-age estimates, males consistently reached lower maximum asymptotic size at a faster rate than females. Based on the gametogenic process, the spawning period was inferred to occur from late summer to early autumn. As commonly found in icefishes, females devoted a great investment to reproduction as gonadosomatic index, egg size, and fecundity. A single female (62 cm TL) spawned about 7730 eggs as large as 3–4 mm. Considering also the results of previous studies conducted in other areas, this species is characterized by a long-lasting pelagic juvenile phase and adults share similar life strategies across the range of their spatial distribution, suggesting the possibility of a single panmictic circum-Antarctic population.

A Comparison of Grass Carp Population Characteristics Upstream and Downstream of Lock and Dam 19 of the Upper Mississippi River

Abstract Grass Carp Ctenopharyngodon idella have been intentionally stocked for aquatic vegetation control across the Midwestern United States for several decades. During the 1970s, escapement of Grass Carp into the Missouri River facilitated their naturalization into much of the Mississippi River basin, including the Upper Mississippi River. Lock and Dam 19 (LD19) in Keokuk, Iowa, is a high-head dam that represents a focal point for naturalized Grass Carp management where populations may differ between upstream and downstream pools as result of limited upstream migration, but potential differences between populations have yet to be evaluated to the best of our knowledge. The objective of this study was to compare the relative abundance, size structure, condition, growth, and recruitment variability of Grass Carp collected upstream and downstream of LD19. We sampled Grass Carp monthly (April–October) during 2014 and 2015 from four locations in the Des Moines River (downstream of LD19) and five locations throughout the Skunk, Iowa, and Cedar rivers (upstream of LD19) using boat electrofishing and trammel net sets. We captured 29 Grass Carp upstream of LD19 compared with 179 individuals captured downstream. Trammel nets only captured Grass Carp downstream of LD19; trammel net catch per unit effort upstream of LD19 was low and ranged from 0.0 to 8.0 fish/net lift (mean ± SE = 0.39 ± 0.13). Electrofishing catch per unit effort ranged from 0.0 to 22.7 fish/h (1.49 ± 0.30) and was higher downstream (2.42 ± 0.30) of LD19 than upstream (0.57 ± 0.07). Grass Carp downstream of LD19 tended to be smaller, younger, of lower body condition, had higher mortality rates, and were slower growing compared with those collected upstream and to populations documented in other systems. Understanding and monitoring adult Grass Carp population characteristics upstream and downstream of LD19 is necessary to determine how they may change in response to ongoing harvest efforts for invasive carps in these river reaches.

Effect of equine chorionic gonadotropin on pregnancy rate in Brown Swiss cows under high altitude conditions

ABSTRACT The objective of the present study was to evaluate the administration of Equine Chorionic Gonadotropin (eCG) in a protocol of fixed-time artificial insemination (FTAI) to increase the pregnancy rate in cows subjected to high altitude conditions. These conditions could generate a syndrome of right-sided heart failure and, as consequence, animals trend to have a low body score condition with a decrease in fertility. The research was conducted with 84 lactating cows (Brown Swiss) randomly into two groups with a body condition score between 2.5 and 3.5, allocated at 3100 m above sea level. In the FTAI protocol, 400 IU of eCG was administered (n = 42) or not (n = 42) at progesterone intravaginal device removal. A significant improvement in pregnancy rate was found with eCG treatment in those cows with low body condition score (BCS of 2.5, scale 1–5), while no effect was found in those cows with higher BCS (i.e. 3.0 and 3.5). In conclusion, the addition of 400 IU of eCG in an FTAI in cows under high altitude conditions and low body conditions can improve the fertility.

How does captivity affect skin colour reflectance of golden mantella frogs?

Coloration is an important trait for social communication in amphibians, being used in intra- and intersexual signalling to express information about individual body condition and health state, amongst other things. The striking colour pattern exhibited by some anuran species are also used in “aposematic” signals to advertise unpalatability to predators. The aim of this study was to investigate how the captive environment affects the colour of golden mantella frogs by comparing captive reared frogs with wild conspecifics. A USB-2000 portable diode-array spectrometer and a xenon strobe light source were used to perform spectrophotometric measurements on captive and wild populations. Hue, chroma and brightness of skin colour were analysed as well as body condition using the scaled mass index. Analyses showed variation among populations, but significant differences were only found between captive and wild populations. Generalised linear mixed models were used to evaluate the effects of body condition on colour variation and showed that animals with lower body condition from one captive population had significantly different coloration than their wild counterparts. Importantly, one captive population was not greatly different in coloration from their wild counterparts – demonstrating that this problem is not inevitable in captivity. These results can have important implications for reintroduction programmes.

The impact of long-term reduced access to cleaner fish on health indicators of resident client fish.

In many mutualisms, benefits in the form of food are exchanged for services such as transport or protection. In the marine cleaning mutualism, a variety of 'client' reef fishes offer 'cleaner' fish Labroides dimidiatus access to food in the form of their ectoparasites, where parasite removal supposedly protects the clients. Yet, the health benefits individual clients obtain in the long term from repeated ectoparasite removal remain relatively unknown. Here, we tested whether long-term reduced access to cleaning services alters indicators of health status such as body condition, immunity and the steroids cortisol and testosterone in four client damselfish species Pomacentrus amboinensis, Amblyglyphidodon curacao, Acanthochromis polyacanthus and Dischistodus perspicillatus To do so, we took advantage of a long-term experimental project in which several small reefs around Lizard Island (Great Barrier Reef, Australia) have been maintained cleaner-free since the year 2000, while control reefs had their cleaner presence continuously monitored. We found that the four damselfish species from reef sites without cleaners for 13 years had lower body condition than fish from reefs with cleaners. However, immunity measurements and cortisol and testosterone levels did not differ between experimental groups. Our findings suggest that clients use the energetic benefits derived from long-term access to cleaning services to selectively increase body condition, rather than altering hormonal or immune system functions.

Cardiac cachexia in cats with congestive heart failure: Prevalence and clinical, laboratory, and survival findings.

BackgroundCardiac cachexia is common in people and dogs with congestive heart failure (CHF). However, the prevalence and effects of cardiac cachexia in cats are unknown.ObjectivesTo determine the prevalence of cachexia and its associations with clinical laboratory and survival data in cats with CHF.AnimalsOne hundred twenty‐five cats with CHF.MethodsMedical records of cats evaluated during a 40‐month period were retrospectively reviewed to identify cats with cardiac cachexia using 7 different definitions. Clinical, laboratory, and survival data were compared between cats with and without cachexia.ResultsPrevalence of cachexia ranged from 0 to 66.7% for the 7 definitions, with a prevalence of 41.6% using muscle condition score (MCS). Cats with cachexia (determined by MCS) were older (P < .001), more likely to have pleural effusion (P = .003), had significantly higher blood urea nitrogen (P < .001) and neutrophil concentrations (P = .01), and significantly lower body condition score (P < .001), body weights (P < .001), hematocrit (P = .007), and hemoglobin concentrations (P = .009). Survival time for cats with cachexia (determined by MCS) was significantly shorter than for cats without cachexia (P = .03). Cats that were underweight (P = .002) and cats with dilated cardiomyopathy (DCM) also had shorter survival times (P = .04).Conclusions and Clinical ImportanceThe association between cachexia and reduced survival time emphasizes the importance of identifying and addressing this common problem in cats with CHF.

Otolith chemistry indicates recent worsened Baltic cod condition is linked to hypoxia exposure

Deoxygenation worldwide is increasing in aquatic systems with implications for organisms' biology, communities and ecosystems. Eastern Baltic cod has experienced a strong decline in mean body condition (i.e. weight at a specific length) over the past 20 years with effects on the fishery relying on this resource. The decrease in cod condition has been tentatively linked in the literature to increased hypoxic areas potentially affecting habitat range, but also to benthic prey and/or cod physiology directly. To date, no studies have been performed to test these mechanisms. Using otolith trace element microchemistry and hypoxia-responding metrics based on manganese (Mn) and magnesium (Mg), we investigated the relationship between fish body condition at capture and exposure to hypoxia. Cod individuals collected after 2000 with low body condition had a higher level of Mn/Mg in the last year of life, indicating higher exposure to hypoxic waters than cod with high body condition. Moreover, lifetime exposure to hypoxia was even more strongly correlated to body condition, suggesting that condition may reflect long-term hypoxia status. These results were irrespective of fish age or sex. This implies that as Baltic cod visit poor-oxygen waters, perhaps searching for benthic food, they compromise their own performance. This study specifically sheds light on the mechanisms leading to the low condition of cod and generally points to the impact of deoxygenation on ecosystems and fisheries.

Elevated CO2 and food ration affect growth but not the size-based hierarchy of a reef fish

Under projected levels of ocean acidification, shifts in energetic demands and food availability could interact to effect the growth and development of marine organisms. Changes to individual growth rates could then flow on to influence emergent properties of social groups, particularly in species that form size-based hierarchies. To test the potential interactive effects of (1) food availability, (2) elevated CO2 during juvenile development, and (3) parental experience of elevated CO2 on the growth, condition and size-based hierarchy of juvenile fish, we reared orange clownfish (Amphiprion percula) for 50 days post-hatching in a fully orthogonal design. Development in elevated CO2 reduced standard length and weight of juveniles, by 9% and 11% respectively, compared to ambient. Development under low food availability reduced length and weight of juveniles by 7% and 15% respectively, compared to high food. Parental exposure to elevated CO2 restored the length of juveniles to that of controls, but it did not restore weight, resulting in juveniles from elevated CO2 parents exhibiting 33% lower body condition when reared in elevated CO2. The body size ratios (relative size of a fish from the rank above) within juvenile groups were not affected by any treatment, suggesting relative robustness of group-level structure despite alterations in individual size and condition. This study demonstrates that both food availability and elevated CO2 can influence the physical attributes of juvenile reef fish, but these changes may not disrupt the emergent group structure of this social species, at least amongst juveniles.

Demonstration of Circulating Antibodies of Mycobacterium avium Subspecies paratuberculosis in Cattle of Rupandehi District, Nepal

Paratuberculosis, caused by Mycobacterium avium subspp. paratuberculosis, is a chronic intestinal infection of global importance in mainly domestic and wild ruminants. The main objective of the study was to find out the seroprevalence of Paratuberculosis in cattle of Rupandehi district. The research was conducted from October 2016 to December 2016. A total of 184 blood samples were collected from Jugular vein of cattle and tested by Enzyme Linked Immunosorbent Assay (ELISA). The Paratuberculosis Indirect Screening Test Kit was developed by ID. Vet, France. Cattle with history of chronic diarrhoea and emaciation were taken as study population along with other cattle in close association with them. Overall seroprevalence in Rupandehi district was found to be 4.89%. No significant relation of paratuberculosis was found with age, breed, parity, body condition score and location. Higher prevalence was found in cattle of older age and low body condition score. The result of this study reports the presence of bovine paratuberculosis in cattle of Rupandehi district.

Sex-specific effects of the in ovo environment on early-life phenotypes in eiders

Maternal effects affect offspring phenotype and fitness. However, the roles of offspring sex-specific sensitivity to maternal glucocorticoids and sex-biased maternal investment remain unclear. It is also uncertain whether telomere length (a marker associated with lifespan) depends on early growth in a sex-specific manner. We assessed whether maternal traits including corticosterone (CORT; the main avian glucocorticoid) and in ovo growth rate are sex-specifically related to offspring CORT exposure, relative telomere length (RTL) and body condition in eiders (Somateria mollissima). We measured feather CORT (fCORT), RTL and body condition of newly hatched ducklings, and growth rate in ovo was expressed as tarsus length at hatching per incubation duration. Maternal traits included baseline plasma CORT, RTL, body condition and breeding experience. We found that fCORT was negatively associated with growth rate in daughters, while it showed a positive association in sons. Lower offspring fCORT was associated with higher maternal baseline plasma CORT, and fCORT was higher in larger clutches and in those hatching later. The RTL of daughters was negatively associated with maternal RTL, whereas that of males was nearly independent of maternal RTL. Higher fCORT in ovo was associated with longer RTL at hatching in both sexes. Duckling body condition was mainly explained by egg weight, and sons had a slightly lower body condition. Our correlational results suggest that maternal effects may have heterogeneous and even diametrically opposed effects between the sexes during early development. Our findings also challenge the view that prenatal CORT exposure is invariably associated with shorter telomeres.

Effects of food deprivation on plasma insulin-like growth factor-1 (Igf1) and Igf binding protein (Igfbp) gene transcription in juvenile cabezon (Scorpaenichthys marmoratus)

The growth hormone (GH)/insulin-like growth factor (Igf) endocrine axis regulates somatic growth in the face of changing environmental conditions. In actinopterygian fishes, food availability is a key modulator of the somatotropic axis, with lower food intake generally depressing liver Igf1 release to diminish growth. Igf1 signaling, however, also involves several distinct IGF binding proteins (Igfbps), and the functional roles of many of these Igfbps in affecting growth during shifting food availability remain uncertain. Here, we tested how complete food deprivation (fasting) affected gene transcription for paralogs of all six types of Igfbps in the liver and fast-twitch skeletal muscle of cabezon (Scorpaenichthys marmoratus), a nearshore marine fish important for recreational fisheries in the eastern North Pacific Ocean. Juvenile cabezon were maintained as either fed (6% mass food⋅g fish wet mass−1⋅d−1) or fasted for 14 d. Fasted fish exhibited a lower body condition (K), a depressed mass-specific growth rate (SGR), and reduced plasma concentrations of Igf1. In the liver, fasting reduced the relative abundance of gene transcripts encoding Igfbps igfbp2a and igfbp2b, while significantly elevating mRNA levels for igfbp1a, igfbp1b, igfbp3b, and igfbp4. Fasting also reduced hepatic mRNA levels of GH receptor-1 (ghr1) – but not GH receptor-2 (ghr2) – supporting the idea that changes in liver sensitivity to GH may underlie the decline in plasma Igf1 during food deprivation. In skeletal muscle, fasting downregulated gene transcripts encoding igf1, igfbp2b, igfbp5b, and igfbp6b, while also upregulating mRNAs for igf2 and ghr2. These data demonstrate isoform-specific regulation of Igfbps in liver and skeletal muscle in cabezon experiencing food deprivation and reinforce the idea that the repertoire of duplicated Igfbp genes that evolved in actinopterygian fishes supports a diverse scope of endocrine and paracrine functions.

Long-Term Effects of Maternal Subnutrition in Early Pregnancy on Cow-Calf Performance, Immunological and Physiological Profiles during the Next Lactation.

Simple SummaryEarly pregnancy is a crucial stage in the fetus development. In this phase, from undifferentiated cells, equal to each other, tissues and organs start to develop. Nutrition and metabolic status of the cow during pregnancy affect the intrauterine environment and the nutrient source for the fetus. Therefore, cow diet during early pregnancy affects the fetus development and could have long-term consequences on the future calf. In this study, we assessed the effects of a poor maternal diet during the first third of gestation on the performance of cows and calves during the next lactation, as well as the effect on the transfer of immunity from cow to calf. We used Parda de Montaña and Pirenaica cow-calf pairs, two Spanish autochthonous beef breeds. We concluded that maternal undernutrition reduced the body fat reserves of cows at calving, which affected most of the cow productive parameters and the colostrum immunoglobulin concentration. Furthermore, poor maternal diet altered the calf development and metabolic status, with reduced size and weight at weaning, especially in the Pirenaica breed, which prioritized the cow maintenance instead of the calf growth.This study aimed to evaluate the effects of undernutrition during the first third of gestation on cow-calf performance, immunological and physiological profiles during the next lactation in two cattle breeds. Fifty-three Parda de Montaña (PA) and 32 Pirenaica (PI) cows were inseminated, assigned to one of two diets (CONTROL or SUBNUT; 100% or 65% of their requirements) until day 82 of gestation, and fed 100% of the requirements during gestation and next lactation. Cow and calf performance were assessed during lactation. Colostrum and cow-calf plasma samples were analyzed to assess the passive transfer of immunoglobulins and to characterize energy metabolism. At calving, SUBNUT cows had a lower body condition score, which impaired most of the cow-calf parameters. All cows had considerable weight losses during lactation except for SUBNUT-PI cows. Colostrum immunoglobulin G (IgG) concentration was lower in SUBNUT-PI cows, and milk fat content was higher in SUBNUT cows. SUBNUT calves had lower values of body measurements at weaning, and calves born from SUBNUT-PI dams had lower milk intake and the lowest average daily gain (ADG), which was reflected in their lower plasma insulin-like growth factor-1 (IGF-1) concentration. In conclusion, undernutrition in early gestation in suckler cows had long-term effects on offspring postnatal growth, this physiological evidence being more severe in Pirenaica cow-calf pairs.

Environmental variation mediates the prevalence and co-occurrence of parasites in the common lizard, Zootoca vivipara

BackgroundHosts and their parasites are under reciprocal selection, leading to coevolution. However, parasites depend not only on a host, but also on the host’s environment. In addition, a single host species is rarely infested by a single species of parasite and often supports multiple species (i.e., multi-infestation). Although the arms race between a parasite and its host has been well studied, few data are available on how environmental conditions may influence the process leading to multiple infestations. In this study, we examine whether: (1) environmental factors including altitude, temperature, vegetation cover, human disturbance, and grazing by livestock affect the prevalence of two types of ectoparasites, mites and ticks, on their host (the common lizard, Zootoca vivipara) and (2) competition is evident between mites and ticks.ResultsWe found the probability of mite infestation increased with altitude and vegetation cover, but decreased with human disturbance and presence of livestock. In contrast, the probability of tick infestation was inversely associated with the same factors. Individuals with low body condition and males had higher mite loads. However, this pattern was not evident for tick loads. The results from a structural equation model revealed that mites and ticks indirectly and negatively affected each other’s infestation probability through an interaction involving the environmental context. We detected a direct negative association between mites and ticks only when considering estimates of parasite load. This suggests that both mites and ticks could attach to the same host, but once they start to accumulate, only one of them takes advantage.ConclusionThe environment of hosts has a strong effect on infestation probabilities and parasite loads of mites and ticks. Autecological differences between mites and ticks, as indicated by their opposing patterns along environmental gradients, may explain the pattern of weak contemporary interspecific competition. Our findings emphasize the importance of including environmental factors and the natural history of each parasite species in studies of host–parasite coevolution.

Predictors of translocation success of captive‐reared lizards: implications for their captive management

AbstractIslands are biodiversity hotspots, but their native inhabitants are vulnerable to predation from exotic predators. Conservation of island endemics has often involved translocating captive‐reared populations to predator‐free refugia. However, the long‐term success of these translocations has rarely been assessed. We investigated the traits that maximize post‐translocation survival in a cohort of captive‐reared Suter's skinks Oligosoma suteri and compared traits normally associated with survival and persistence of lizards (body condition, speed and overall size) to that of wild‐born skinks 6 years after release onto a predator‐free island in the north‐east of New Zealand. Our models showed that larger lizards, and lizards with lower body condition, had improved survival. While sprint speed of captive‐reared lizards did not differ significantly to that of wild skinks, diving ability of captive‐reared skinks was poor, with only female captive‐reared lizards diving during trials. Our results indicate that the traits associated with higher survival after release are not necessarily obvious and may be influenced by adaptation to captive conditions. Long‐term monitoring post‐translocation is therefore vital to determine the success of the translocation.

Testosterone and Corticosterone Profiles and Body Condition of Calling and Non-calling Lithobates grylio

Anuran vocalization is one of the most energetically expensive behaviors recorded in vertebrates. To sustain this behavior, circulating hormones in male frogs must act to promote calling behavior while mobilizing energy reserves. We hypothesize that this is accomplished through the actions of testosterone (T) and corticosterone (CORT). T is thought to elicit calling, while CORT mobilizes energy reserves. Eventually, high CORT concentrations and low energy reserves suppress calling behavior. We evaluated this hypothesis in light of the reproductive ecology of the Pig Frog, Lithobates grylio. Plasma samples from calling and non-calling males were collected during four months of the reproductive season. Calling males were shown to have significantly higher concentrations of circulating T and high body conditions. Conversely, non-callers exhibited elevated CORT and low body conditions. There was no correlation between the two hormones, but circulating T concentrations did decrease over the season. Lastly, we found that CORT is positively correlated with circulating glucose (in non-callers); this finding is supported by CORT's known role in gluconeogenesis. Taken together, these data indicate that circulating hormones and available energy reserves likely influence calling behavior in L. grylio.

Risk factors associated with lameness and hoof lesions in pasture-based dairy cattle systems in southeast Brazil

Cattle lameness is an important welfare concern that also has an economic impact on the dairy industry. It can be a significant problem among pasture-based herds. Our objectives were to identify cow- and herd-level factors related to lameness and hoof lesions in dairy cows grazing year-round in Minas Gerais, Brazil. We performed a cross-sectional study in 48 pasture-based dairy herds, visiting each farm in a single visit. We evaluated 2,262 cows for mobility score (0-3) and 392 cows for hoof lesions. We used a questionnaire and checklist to capture herd management data. All information obtained was used to build multivariable models. The factors associated with lameness were low body condition score, longer time spent in the corral, being kept in paddocks during the drought period, and poor hygiene. For hoof lesions, track features were the most significant factor in determining the likelihood of heel horn erosion, white line fissure, and sole hemorrhage-by more than 3 times. Different factors related to unhygienic conditions such as leg cleanliness, frequency of cleaning, and longer time spent in the corral were associated with infectious hoof lesions. Poor human-animal relationship was related to sole hemorrhage, but patient handling of cows on the track was a protective factor against interdigital hyperplasia. The results of this study suggest that improving hygiene conditions, track features, and cow handling can improve dairy cattle mobility scores in pasture-based farms under tropical conditions. These findings also represent a first step toward planning actions aimed at decreasing lameness and hoof lesions in the studied region.

Associations between Pre-Slaughter and Post-Slaughter Indicators of Animal Welfare in Cull Cows.

The objective of this study was to evaluate the welfare of cull cows in a slaughtering plant, using indicators of health on arrival and indicators of handling during the stunning process. These pre-slaughter indicators were associated with post-slaughter indicators of the same cows, such as carcass bruising and condemnations. Transport staff surveys showed that all drivers had been trained on animal welfare. All loads of cows came directly from farms and had an average transport duration of 5 h 22 min. Indicators were registered in 237 cows during unloading at the slaughterhouse and in the stunning box. Bruises and condemnations were recorded post-slaughter in the carcasses of the same cows. Results at arrival showed that 48% of the cows had low body condition, 50% had mammary problems, and 24% suffered from lameness. During stunning, 16% of cows needed a second shot, and 54% exceeded the 60 s established as a recommended interval between stunning and bleeding. During the post-slaughter evaluation, 50% of the carcasses had more than two bruises and 70.46% had a bruise severity score different from zero. Low body condition was a risk factor to increase the severity of bruises; low body condition and mammary problems increased carcass condemnations; the stunning process indicators were not statistically associated with the severity of the bruises. For cull cows the main animal welfare issue originates at farm level.

Prevalence, risk factors and constraints in control of repeat breeding in dairy animals of Punjab

The present study investigated the occurrence of repeat breeding (RB) and constraints in controlling this disease in cattle and buffalo in Punjab. Livestock owners/households (720) constituted the ultimate sampling units for the study. The dairy farmers were categorized into small (5–10 animals), medium (11–50) and large (&gt;50) farmers. The overall prevalence of repeat breeding in the study area was 27.6%. It varied significantly with respect to herd size and categories of animals except in buffaloes. The highest prevalence was observed in crossbred cattle (41.8%), followed by indigenous cattle (32.0%) and buffaloes (17.6%). Repeat breeder animals were mostly high yielders (with &gt;15 kg milk yield), calved mostly in winter season, having below average body condition score (BCS). No significant correlation was observed between parity (lactation number) and type of calving and prevalence of repeat breeding. The major constraint (rank I) perceived by farmers was culling of affected problematic cows, and lack of effective and accurate estrus detection in buffaloes. The other major constraints perceived in the study area were high cost of veterinary medicine, poor conception rate following artificial insemination and inaccessibility of qualified veterinarian. The lack of knowledge regarding animal nutrition and higher cost of branded feed available in the market were responsible for low body condition score of affected animals. Thus, a comprehensive policy and programmes involving various stakeholders are required for effective control of repeat breeding in Punjab.

Landscape homogenization due to agricultural intensification disrupts the relationship between reproductive success and main prey abundance in an avian predator

BackgroundSelecting high-quality habitat and the optimal time to reproduce can increase individual fitness and is a strong evolutionary factor shaping animal populations. However, few studies have investigated the interplay between land cover heterogeneity, limitation in food resources, individual quality and spatial variation in fitness parameters. Here, we explore how individuals of different quality respond to possible mismatches between a cue for prey availability (land cover heterogeneity) and the actual fluctuating prey abundance.ResultsWe analyse timing of breeding and reproductive success in a migratory population of Eurasian kestrels (Falco tinnunculus) breeding in nest-boxes, over a full three-year abundance cycle of main prey (voles), and consider several components of individual quality, including body condition, blood parasite infection, and genetic diversity (n = 448 adults) that act on different time scales. Older individuals, and kestrel parents in higher body condition started egg-laying earlier than younger birds and those in lower body condition. Additionally, egg-laying was initiated earlier during the increase and decrease phases (2011 and 2012) than during the low phase of the vole cycle (2013). Nestling survival (ratio of eggs that fledged successfully) was higher in early nests and in heterogeneous landscapes (i.e., mosaic of different habitat types), which was evident during the increase and decrease phases of the vole cycle, but not during the low vole year.ConclusionsWe found a strong positive effect of landscape heterogeneity on nestling survival, but only when voles were relatively abundant, whereas a difference in the timing of breeding related to territory landscape heterogeneity was not evident. Therefore, landscape heterogeneity appeared as the main driver of high reproductive performance under favourable food conditions. Our results show that landscape homogenization linked to agricultural intensification disrupts the expected positive effect of vole abundance on reproductive success of kestrels.