Objective: To compare the efficacy of anal adenectomy with virtual hanging wire and anal fistulotomy in the treatment of low anal fistula in infants and children. Methods: 60 children with low anal fistula who were admitted to our hospital from October 2021 to March 2022 and met the inclusion criteria were randomly divided into two groups of 30 cases each; the treatment group was treated with anal adenectomy and virtual hanging wire surgery, and the control group was treated with anal fistula resection. The clinical efficacy after treatment was compared. Results: The total effective rate of both groups was 96.67% and the difference between the two groups was not statistically significant (P > 0.05). The postoperative pain score of the treatment group was lower than that of the control group (P < 0.05). The length of hospitalization and healing time of the treatment group was lower than that of the control group (P < 0.05). The anal function of the patients in both groups was normal, and there was no adverse reaction. Conclusion: Anal gland excision and virtual hanging surgery for the treatment of low anal fistula in infants and children have the advantages of mild pain, reduced length of hospitalization, short healing time, and better patient experience as compared to anal fistula excision.