Surface wetting occurring in daily life causes undesired contaminations, which are critical issues in various fields. To solve these problems, the nonwetting property of a superhydrophobic (SH) surface has proven its utility by preventing contaminant infiltration, serious infections, or malfunction. However, the application of SH surfaces in the biomedical field has been limited due to the weak durability and toxicity of the related components. To overcome these limitations, we developed a robust and biocompatible SH surface through combinational biomimicking of three natural organisms, lotus leaf, mussel, and sandcastle worm, for the first time. Using the water-immiscible and polycationic characteristics of mussel adhesive protein (iMglue), an SH iMglue-SiO2(TiO2/SiO2)2 coating was fabricated by solution-based electrical charge-controlled layer-by-layer growth of nanoparticles (NPs). The fabricated iMglue-SiO2(TiO2/SiO2)2 SH surface showed excellent durable nonwetting properties and was applied to an intracatheter tube coating to develop antithrombotic catheters under blood flow. Furthermore, we developed a iMglue-employed SH patch for a tissue closure bandage by spraying hydrophobic SiO2 NPs on the iMglue-covered cotton pads. The prepared iMglue-employing SH patch showed perfect bifunctionality with excellent antibiofouling and tissue closure capabilities. Our work presents a novel, useful strategy for fabricating a biomedically multifunctional, robust SH surface through combinational mimicking of natural organisms.