Loss Of Ovarian Tissue Research Articles

Morphofunctional Features of the Ovaries in Benign Small Cystic Tumors

The aim of the study was to analyze the effect of surgical intervention on the status of the ovarian follicular apparatus in patients with benign ovarian cystic tumors (BCOTs) based on the results of morphometric studies.Material and methods. The authors have studied surgical material of 60 patients aged 18-47 years with BCOTs: serous cystadenoma (20 cases), serous papillary cystadenoma (7 cases), mucinous cystadenoma (12 cases), mature teratoma (23 cases). Microscopic slides of ovarian tissue stained with hematoxylin and eosin were obtained and morphological structure of BCOTs, presence of the intact ovarian tissue surrounding neoplasm and the density of follicles there were studied based on their qualitative characteristics (maturity, signs of degeneration).Results. It was found out that development of fibrosis in the wall of BCOTs significantly depends on the tumor histogenesis: the greatest thickness of fibrosis was detected in serous papillary cystadenomas, the smallest in the group of mature teratomas, the average values were determined in mucinous and serous cystadenomas of the ovaries. The influence of BCOTs on the ovarian reserve does not depend on its morphological structure, localization and diameter. The intact ovarian tissue is removed along with BCOTs during adnexectomy, ovarian resection and in 48.8% of cases – during cystectomy. During cystectomy, probability of the perifocal intact ovarian tissue presence is reduced due to the growth of fibrous tissue in this area. However, the negative impact of BCOTs on the ovarian reserve occurs not only due to surgical removal of the ovarian tissue, but also as a result of inhibition of folliculogenesis at the preoperative stage.Conclusion. BCOTs of the presented morphological groups negatively affect the follicular apparatus due to both – inhibition of folliculogenesis at the preoperative stage and loss of ovarian tissue during surgery. More pronounced perifocal proliferation of fibrous tissue was noted with serous papillary cystadenomas.

Influence of Endomeriotic Cyst Diameter and the Severity of Endometriosis on the Ovarian Parenchyma Excised During Laparoscopic Cystectomy

Endometrioma is a common form of endometriosis and it is represented by endometriotic deposits within the ovaries which accounts for upto 17-44% of cases. Laparoscopic stripping of ovarian endometriotic cysts is an accepted technique owing to low recurrence rates. Decrease in residual ovarian tissue volume was noted in many studies followed cystectomy. This study aims at estimating whether the size of endometriotic cyst is related to ovarian parenchyma excised along with cyst wall. Prospective study was done at University teaching hospital for one year. A total of 56 women underwent laparoscopic endometriotic cystectomy. Cystectomy was done by stripping method and endometriosis was staged according to revised American Society of Reproductive Medicine classification (rASRM). The endometrioma wall was evaluated histologically and were categorised into 2 groups based on semi- quantitative scale of 0-4. Group 1 and 2 showed grade 0, 1, 2 and 3, 4 in the cyst wall respectively. Mean age of patients was 31.4 years, duration of infertility was 4.1 years and cyst diameter measured 6.3 cm. 73.2% (n=41) were in Group 1 and 26.8% (n=15) were in Group 2 , mean cyst diameter was being 4.3 cm and 5.0 cm respectively. There was no significant statistical correlation between preoperative cyst diameter and ovarian parenchyma removed (p=0.15). 93.3% (n=14) of group 2, and only 65.8% (n=27 ) of group 1 were found to have moderate to severe endometriosis, indicating there is correlation between disease severity and loss of ovarian tissue (p= 0.04). Endometriotic cystectomy when performed with accurate surgical technique leads to no significant ovarian tissue removal. However, disease severity significantly determines the loss of normal ovarian parenchyma.

The structure of the gonad during natural sex reversal in Monopterus albus (Pisces: Teleostei)

A detailed study on the structure of the gonad of Monopterus albus was made as a basis for analysis of gonadal steroids in this sex‐reversing teleost. Two types of males were identifiedand their existence appeared to be a result of the difference in gonadal ontogeny among the individuals in natural populations. The germinal area of the gonad, the gonadal lamellae, exhibited à zoned nature with regard to the location of the female and male germ cells. Observations suggested that the male germ cells originated from gonia pre‐existing in the inner zone of the gonadal lamellae before sex reversal. Natural reversal of sex in this protogynous hermaphrodite was found to be usually a postnuptial event and was always accompanied by loss of ovarian tissue and by development of interstitial (Leydig) cells. In the mesogonial region of the gonadal wall, peculiar mesenchyme cells were found, their significance remained uncertain.