Production Losses Research Articles

Prevalence of and potential risk factors for multiple resistance to acaricides in Rhipicephalus (Boophilus) microplus ticks: A survey in the state of Rio Grande Do Sul, Brazil.

The cattle tick Rhipicephalus (Boophilus) microplus (Canestrini, 1887) (Ixodida, Ixodidae) is responsible for significant economic losses in bovine production in tropical and subtropical regions worldwide. Control of this tick predominantly involves the use of chemical acaricides; however, their indiscriminate use has led to the selection of resistant populations. A survey on tick populations was conducted in the state of Rio Grande do Sul, in Brazil, to assess the prevalence of multiple resistance to acaricides in cattle farms. Additionally, questionnaires were administered to identify potential risk factors associated with multiple resistance to acaricides. In total, 176 farms with a bovine population of ≥40 cattle were randomly assigned for tick sampling. The resistance to six acaricidal compounds was investigated by bioassays. A larval packet test was performed for amitraz, chlorpyrifos, cypermethrin, fipronil and ivermectin. Fluazuron was screened using an adult immersion test. Multiple resistance to acaricides (i.e., resistance to three or more compounds) was detected in 173 samples, representing 98% of the total samples. Among these samples, 125 (71%) showed resistance to all six compounds tested. Additionally, we classified the resistance intensity into four levels (I to IV) based on the quartile distribution of the bioassay data. Ten samples (6%) showed high and very high levels (III and IV) of resistance to all six compounds tested. Three variables were significantly associated with multiple resistance to the six acaricides tested: (i) use of injectable acaricides to control ticks, (ii) application of more than five acaricide treatments per year, and (iii) farms with larger herds (≥232 animals). These results regarding widespread resistance and the emergence of multiple resistance to acaricides ticks are alarming and highlight the significant challenge of tick control in southern Brazil.

Unlocking the power of tobacco taxation to mitigate the social costs of smoking in Mexico: a microsimulation model.

Despite being the most cost-effective tobacco control policy, tobacco taxation is the least implemented component of the World Health Organization MPOWER package to reduce smoking worldwide. In Mexico, both smoking prevalence and taxation have remained stable for more than a decade. This study aims to provide evidence about the potential effects of taxation to reduce the burden of tobacco-related diseases and the main attributable social costs in Mexico, including informal (unpaid) care costs, which are frequently ignored. We employ a first-order Monte Carlo microsimulation model that follows hypothetical population cohorts considering the risks of an adverse health event and death. First, we estimate tobacco-attributable morbidity and mortality, direct medical costs and indirect costs, such as labour productivity losses and informal care costs. Then, we assess the potential effects of a 50% cigarette price increase through taxation and two alternative scenarios of 25% and 75%. The inputs come from several sources, including national surveys and vital statistics. Each year, 63 000 premature deaths and 427 000 disease events are attributable to tobacco in Mexico, while social costs amount to MX$194.6 billion (US$8.5)-MX$116.2 (US$5.1) direct medical costs and MX$78.5 (US$3.4) indirect costs-representing 0.8% of gross domestic product. Current tobacco tax revenue barely covers 23.3% of these costs. Increasing cigarette prices through taxation by 50% could reduce premature deaths by 49 000 over the next decade, while direct and indirect costs averted would amount to MX$87.9 billion (US$3.8) and MX$67.6 billion (US$2.9), respectively. The benefits would far outweigh any potential loss even in a pessimistic scenario of increased illicit trade. Tobacco use imposes high social costs on the Mexican population, but tobacco taxation is a win-win policy for both gaining population health and reducing tobacco societal costs.

Ironies of Generative AI: Understanding and Mitigating Productivity Loss in Human-AI Interaction

Generative AI (GenAI) systems offer opportunities to increase user productivity in many tasks, such as programming and writing. However, while they boost productivity in some studies, many others show that users are working ineffectively with GenAI systems and losing productivity. Despite the apparent novelty of these usability challenges, these ‘ironies of automation’ have been observed for over three decades in Human Factors research on the introduction of automation in domains such as aviation, automated driving, and intelligence. We draw on this extensive research alongside recent GenAI user studies to outline four key reasons for productivity loss with GenAI systems: a shift in users’ roles from production to evaluation, unhelpful restructuring of workflows, interruptions, and a tendency for automation to make easy tasks easier and hard tasks harder. We then suggest how Human Factors research can also inform GenAI system design to mitigate productivity loss by using approaches such as continuous feedback, system personalization, ecological interface design, task stabilization, and clear task allocation. Thus, we ground developments in GenAI system usability in decades of Human Factors research, ensuring that the design of human-AI interactions in this rapidly moving field learns from history instead of repeating it.

Potato Leaf Disease Detection Based on a Lightweight Deep Learning Model

Traditional methods of agricultural disease detection rely primarily on manual observation, which is not only time-consuming and labor-intensive, but also prone to human error. The advent of deep learning has revolutionized plant disease detection by providing more accurate and efficient solutions. The management of potato diseases is critical to the agricultural industry, as these diseases can lead to substantial losses in crop production. The prompt identification and classification of potato leaf diseases are essential to mitigating such losses. In this paper, we present a novel approach that integrates a lightweight convolutional neural network architecture, RegNetY-400MF, with transfer learning techniques to accurately identify seven different types of potato leaf diseases. The proposed method not only enhances the precision of potato leaf disease detection but also reduces the computational and storage demands, with a mere 0.40 GFLOPs and a model size of 16.8 MB. This makes it well-suited for use on edge devices with limited resources, enabling real-time disease detection in agricultural environments. The experimental results demonstrated that the accuracy of the proposed method in identifying seven potato leaf diseases was 90.68%, providing a comprehensive solution for potato crop management.

Assessing disease control in inflammatory bowel disease: a real world cross-sectional study in the UK (PODCAST-IBD)

Objective Proportion Of suboptimal Disease Control And Strategy of Treatment in IBD (PODCAST-IBD) was an international real-world study which aimed to quantify disease control in IBD using STRIDE-II recommendations. Design/Method Cross-sectional assessment of IBD patients attending routine clinic appointments in four UK centers October 2022 to January 2023. Clinician-reported outcomes, patient-reported outcomes and retrospective data from medical chart review were used to assess IBD control against red flags aligned to STRIDE-II. Results Data were available from 198 UK patients. IBD was suboptimally controlled in 52.4% (54/103) of patients with Crohn’s disease (CD) and 45.3% (43/95) with ulcerative colitis (UC). Impaired quality of life (QOL), defined as Short inflammatory bowel disease questionnaire (SIBDQ) score <50, was the main contributor to suboptimal disease control. Suboptimal disease control has a detrimental impact on fatigue and disability with significantly lower mean Functional Assessment of Chronic Illness Therapy – Fatigue (FACIT-F) score in suboptimally controlled disease (CD: 81.5 vs 125, UC: 87.4 vs 122.8) and IBD Disk. Suboptimal disease control results in higher health care resource use (HCRU) (CD: £4,746 vs £1,924; UC: £2,428 vs £1,121) and higher rates of work productivity loss (CD: 41.7% vs 11.9%, UC: 38.0% vs 22.6%). Conclusion IBD was suboptimally controlled in around one-half of patients. Impaired QOL was the most common contributor (64%, 62/97) to suboptimal control. Suboptimal control had a considerable economic impact; HCRU more than doubled and productivity fell. Physicians could consider regular QOL assessments to prompt timely disease monitoring to enable identification of early active disease and appropriate treatment.

Relations of magnetic and electrochemical anti-corrosion properties of (Zr, Ti)-doped NdCeFeB magnets with Ti content

Zr and Ti were co-doped into NdCeFeB sintered magnets to improve the magnetic and electrochemical anti-corrosion properties. Both performance are found closely associated with Ti content. At 0.15 wt%Ti, the sample shows higher coercivity without energy product loss compared to the Ti-free sample and other Ti additions, due to the matrix-phases refinement and optimized distribution of RE-rich phases. For this sample, its corrosion current density and charge transfer resistance are 1.94 μA/cm2 and 1734 Ω•cm2 in NaCl solution respectively, which exhibits obviously improved corrosion resistance in comparison with the Ti-free magnet. The charge transfer resistance has been increased by 45.6 %. The apparently improved corrosion resistance is mainly attributed to the decreased amount of active reaction channels and the enhanced protective effect of TiO2, arising from the increment of Ti-rich precipitates. When the content of Ti dopant exceeds 0.5 wt%, the beneficial effects of Ti are overshadowed by the harmful effect of the density reduction on anti-corrosion performance, and the charge transfer resistance decreased with further increasing the dopant content. The new findings provide a theoretical basis for improving physicochemical properties of NdCeFeB magnet.

Cost-effectiveness and cost-utility of optimized mercaptopurine treatment versus placebo in ulcerative colitis patients: The randomized controlled OPTIC trial.

We assessed the cost-effectiveness and cost-utility of therapeutic drug monitoring (TDM)-guided mercaptopurine treatment compared with placebo in ulcerative colitis (UC) patients failing 5-aminosalicylates. Data were gathered alongside the randomized controlled OPTIC trial (EudraCT: 2015-005260-41). The evaluation was performed from a health care and societal perspective as cost-effectiveness and cost-utility analyses with a time horizon of one year. Volumes and costs of in-hospital care, out-of-hospital care, out-of-pocket expenses and productivity loss were assessed. The main outcomes were the extra costs per additional patient who achieved clinical remission and endoscopic improvement at 52weeks (responders) and extra costs per quality-adjusted life-year (QALY) gained. In total, 59 patients were randomized to the intervention (n=29) and control (n=30) group. Non-significant differences in costs were €63 (-€1267 to €1434; P=0.93) in favour of placebo from a health care perspective and -€742 (-€3683 to €2016; P=0.64) in favour of mercaptopurine from a societal perspective. The higher proportion of responders and a non-significant QALY difference of 0.0475 (-0.024-0.117) (P=0.184) favouring patients on mercaptopurine treatment resulted in €165 extra costs per additional responder and €1326 extra costs per QALY gained from a health care perspective. From a societal perspective, dominance over placebo was observed with cost savings of €1937 per additional responder and €15,621 per QALY gained. The probability of optimised mercaptopurine treatment being cost-effective was 0.80 at a willingness to pay per additional QALY of €20,000. TDM-based mercaptopurine treatment in UC patients failing 5-aminosalicylates is a cost-effective strategy from a societal perspective.

Productivity of Wheat Landraces in Rainfed and Irrigated Conditions under Conventional and Organic Input in a Semiarid Mediterranean Environment

Wheat landraces are locally adapted populations that are suitable for low-input agronomic management and constraining pedo-climatic conditions. The productivity of landraces under high-input and optimal conditions is usually lower than modern wheat varieties. The present study compared the response of Sicilian wheat landraces and modern varieties to organic management, including organic fertilization, and conventional management, including mineral fertilization and chemical weed control, under rainfed condition and supplementary irrigation in a field trial conducted on a xerofluvent soil in a semiarid Mediterranean climate. Modern varieties were on average more productive than landraces, although certain landraces achieved comparable yields, in particular under organic management. The increase in grain yield under conventional management in comparison with the organic management was higher for modern varieties than landraces. The loss of productivity in rainfed conditions was lower for landraces compared to modern varieties. The grain quality traits were similar between landraces and modern varieties and in both cases the conventional management led to an improvement of the traits. These findings highlight the resilience and adaptability of traditional wheat landraces to low-input agricultural systems and offer valuable insights into improving the sustainability and productivity of wheat production in Mediterranean environments.

Differential evaluation of neuromuscular injuries to understand re-innervation at the neuromuscular junction

Peripheral nerve-crush injury is a well-established model of neuromuscular junction (NMJ) denervation and subsequent re-innervation. Functionally, the skeletal muscle follows a similar pattern as neural recovery, with immediate loss of force production that steadily improves in parallel with rates of re-innervation. On the other hand, traumatic injury to the muscle itself, specifically volumetric muscle loss (VML), results in an irrecoverable loss of muscle function. Recent work has indicated significant impairments to the NMJ following this injury that appear chronic in nature, alongside the lack of functional recovery. Thus, the goal of this study was to compare the effects of nerve and muscle injury on NMJ remodeling. Even numbers of adult male and female mice were used with three experimental groups: injury Naïve, nerve crush, and VML injury; and three terminal timepoints: 3-, 48-, and 112-days post-injury. Confirming the assumed recoverability of the two injury models, we found in vivo maximal torque was fully restored following nerve-crush injury but remained at a significant deficit following VML. Compared to injury Naïve and nerve-crush injury, we found VML results in aberrantly high trophic signaling (e.g., neuregulin-1) and numbers of supporting cells, including terminal Schwann cells and sub-synaptic nuclei. In some cases, sex differences were detected, including higher rates of innervation in females than males. Both nerve crush and VML injury display chronic changes to NMJ morphology, such as increased fragmentation and nerve sprouting, highlighting the potential of VML for modeling NMJ regeneration in adulthood, alongside the established nerve-injury models.

Catalysing vaccines and biologics manufacturing in Africa

Catalysing vaccines and biologics manufacturing in Africa Professor Faith Osier, Director of the Chanjo Hub at Imperial College London, shares her vision for vaccines and biologics manufacturing in Africa to secure lives and livelihoods and drive economic growth. The COVID-19 pandemic demonstrated that Africa should not rely on donations from rich countries. Africa has the highest burden of endemic infectious diseases globally, accounting for &gt;227mn years of health life lost, an annual productivity loss of &gt;$800bn and an infection-related mortality of ~10m/ year. The list of diseases is long, diverse and challenging. It includes infectious diseases like cholera, Ebola, meningitis, measles, yellow fever, mpox and COVID-19, to mention a few. Some are caused by zoonotic pathogens that spill over from animals to humans. The need for local capacity to rapidly respond to known and unknown threats and to mitigate global impacts is urgent.

Cancer impact on lower-income patients in Malaysian public healthcare: An exploration of out-of-pocket expenses, productivity loss, and financial coping strategies.

Cancer patients often grapple with substantial out-of-pocket (OOP) expenses and productivity loss, with the ramifications being particularly crucial for lower-income households. This study aims to estimate OOP costs incurred by cancer patients, assess their productivity loss, and analyse the financial coping mechanisms employed by individuals within the lower-income bracket. The study employed face-to-face interviews among cancer patients aged 40 years and above, currently undergoing treatment, and belonging to the lower-income group. Participants were recruited from six public cancer referral hospitals. OOP expenses, encompassing medical and non-medical costs, along with productivity loss, were measured. A generalized linear model was applied to identify potential OOP determinants. Additionally, the coping mechanisms employed by individuals to finance their cancer OOP expenses were also determined. Among the 430 participants recruited, predominantly female (63.5%), and aged 60 or older (53.9%). The annual mean total cancer costs per patient were US$ 2,398.28 (±2,168.74), including 15% for medical costs US$ 350.95 (±560.24), 34% for non-medical costs US$820.24 (±818.24), and 51% for productivity loss costs US$1,227.09 (±1,809.09). Transportation, nutritional supplements, outpatient treatment, and medical supplies were notable cost contributors to total OOP expenditures. Ethnicity (β = 1.44; 95%CI = 1.15-1.79), household income (β = 1.40; 95%CI = 1.10-1.78), annual outpatient visits (β = 1.00; 95%CI = 1.00-1.01), age (β = 0.74; 95%CI = 0.56-0.98), and employment status (β = 0.54; 95%CI = 0.72-1.34) were identified as significant predictors of OOP costs among cancer patients. Notably, 91% of participants relied on household salaries and savings, while 15% resorted to interest-free borrowing, 11% sold possessions, and 0.5% borrowed with interest to finance their expenses. This study offers crucial insights into the economic impact of cancer on individuals and their families, providing policymakers with valuable information to tackle challenges faced in their journey. Despite substantial public healthcare subsidies, the study revealed that cancer costs can remain a potential barrier to accessing essential treatment. Therefore, there is a need for reinforced system-level infrastructure to facilitate targeted financial navigation services.

Economic Burden of Community-Acquired Antibiotic-Resistant Urinary Tract Infections: Systematic Review and Meta-Analysis.

Antibiotic resistance (ABR) poses a major burden to global health and economic systems. ABR in community-acquired urinary tract infections (CA-UTIs) has become increasingly prevalent. Accurate estimates of ABR's clinical and economic burden are needed to support medical resource prioritization and cost-effectiveness evaluations of urinary tract infection (UTI) interventions. This study aims to systematically synthesize the evidence on the economic costs associated with ABR in CA-UTIs, using published studies comparing the costs of antibiotic-susceptible and antibiotic-resistant cases. We searched the PubMed, Ovid MEDLINE and Embase, Cochrane Review Library, and Scopus databases. Studies published in English from January 1, 2008, to January 31, 2023, reporting the economic costs of ABR in CA-UTI of any microbe were included. Independent screening of titles/abstracts and full texts was performed based on prespecified criteria. A quality assessment was performed using the Integrated Quality Criteria for Review of Multiple Study Designs (ICROMS) tool. Data in UTI diagnosis criteria, patient characteristics, perspectives, resource costs, and patient and health economic outcomes, including mortality, hospital length of stay (LOS), and costs, were extracted and analyzed. Monetary costs were converted into 2023 US dollars. This review included 15 studies with a total of 57,251 CA-UTI cases. All studies were from high- or upper-middle-income countries. A total of 14 (93%) studies took a health system perspective, 13 (87%) focused on hospitalized patients, and 14 (93%) reported UTI pathogens. Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa are the most prevalent organisms. A total of 12 (80%) studies reported mortality, of which, 7 reported increased mortality in the ABR group. Random effects meta-analyses estimated an odds ratio of 1.50 (95% CI 1.29-1.74) in the ABR CA-UTI cases. All 13 hospital-based studies reported LOS, of which, 11 reported significantly higher LOS in the ABR group. The meta-analysis of the reported median LOS estimated a pooled excess LOS ranging from 1.50 days (95% CI 0.71-4.00) to 2.00 days (95% CI 0.85-3.15). The meta-analysis of the reported mean LOS estimated a pooled excess LOS of 2.45 days (95% CI 0.51-4.39). A total of 8 (53%) studies reported costs in monetary terms-none discounted the costs. All 8 studies reported higher medical costs spent treating patients with ABR CA-UTI in hospitals. The highest excess cost was observed in UTIs caused by carbapenem-resistant Enterobacterales. No meta-analysis was performed for monetary costs due to heterogeneity. ABR was attributed to increased mortality, hospital LOS, and economic costs among patients with CA-UTI. The findings of this review highlighted the scarcity of research in this area, particularly in patient morbidity and chronic sequelae and costs incurred in community health care. Future research calls for a cost-of-illness analysis of infections, standardizing therapy-pathogen combination comparators, medical resources, productivity loss, intangible costs to be captured, and data from community sectors and low-resource settings and countries.

Efficacy of Alternative Insecticides against Dusky Cotton Bug (Oxycarenus laetus) to Improve Yield Losses in Cotton Crops through Residue-based Bioassay

The study evaluates the efficacy of leufenuron, emamectin benzoate, and thiamethoxam against the Dusky Cotton Bug (Oxycarenus laetus Kirby) using residue-based bioassay methods. Key findings indicate that emamectin benzoate showed the highest efficacy with the lowest LC50 value, making it the most potent insecticide among those tested. Leufenuron and thiamethoxam followed, displaying moderate effectiveness. The results highlight the comparative advantages of emamectin benzoate in controlling Dusky Cotton Bug populations, suggesting its potential role in integrated pest management strategies. This study underscores the need for environmentally friendly alternatives to traditional insecticides in mitigating yield losses in cotton production.

Long-term alien marsh grass in China brings high carbon uptake capacity but cannot sustain high-temperature weather

Coastal wetlands, crucial in the global carbon cycle, face increasing challenges brought by extreme climate events, particularly high temperatures above plant tolerance thresholds. These conditions often exert great impact on plant, thereby potentially reducing overall ecosystem productivity. However, it has been observed that alien species, typically exhibiting higher productivity compared to native plant. Would plant invasion offset the loss of productivity caused by high-temperature events at ecosystem scale? In this study, we utilized data from 2020 to 2023 in China's Yangtze Estuary to investigate the responses of Spartina alterniflora (alien) and Phragmites australis (native) to high-temperature stress. Our results demonstrate that though the alien vegetation exhibits higher productivity before high temperature events, it experiences significant declines during high temperatures. In average, net ecosystem productivity (NEP) and gross primary productivity (GPP) of alien plant drops by 21.03% overall, with a notable 29.59% reduction during Neap tide. In contrast, native vegetation maintains a more stable productivity profile under the same conditions. Spring tide alleviate the negative impact of high temperatures on the alien vegetation, exhibiting a distinct environmental buffering effect. Photosynthetic photon flux density emerged as a crucial factor driving productivity, yet its effectiveness was moderated by aerodynamic conductance for heat transfer (Ga_h). Through the application of the Michaelis-Menten model, we confirmed that both species maintain similar maximum light utilization efficiencies, yet native vegetation demonstrates greater resilience to thermal stress. Additionally, we observed a 33.82% overestimation in productivity by the vegetation photosynthesis model (VPM) under high temperatures, emphasizing the need to refine how Ga_h impacts are integrated, particularly when comparing the resilience of native and alien species. We emphasize necessity of incorporating canopy structure factors into ecological models and underscore the importance of maintaining tidal dynamics for coastal wetland management.

An asymptomatic geminivirus activates autophagy and enhances plant defenses against diverse pathogens

Plant viral diseases cause great losses in agricultural production. Virus cross-protection is a strategy in which a mild virus is employed to shield plants against subsequent infections by severe viral strains. However, this approach is restricted to protection against the same viruses. In this study, we observed that pre-inoculation with apple geminivirus (AGV) reduced the accumulation of secondarily infected heterologous viruses, such as cucumber mosaic virus, potato virus X, and tobacco mosaic virus in Nicotiana benthamiana, tomato, and pepper plants. Transcriptional expression analysis showed that autophagy-related genes were transcriptionally up-regulated upon AGV inoculation at an early stage of infection. Accordingly, autophagic activity was observed to be elevated following AGV infection. Interestingly, AGV accumulation was reduced in autophagy-deficient plants, suggesting that autophagy activation promotes AGV infection in the plant. Moreover, pre-inoculation with AGV provided cross-protection against infection with a phytopathogenic bacterium (Pseudomonas syringae) and fungus (Botrytis cinerea) in Nicotiana species. In summary, our study showed that AGV, an asymptomatic virus, could protect plants against severe viral, fungal, and bacterial diseases to some extent through the activation of autophagy pathways, highlighting its potential as a biocontrol agent for managing a wide range of plant crop diseases in the field.

Quantification of the fungal pathogen Didymella segeticola in Camellia sinensis using a DNA-based qRT-PCR assay.

The fungal pathogen Didymella segeticola causes leaf spot and leaf blight on tea plant (Camellia sinensis), leading to production losses and affecting tea quality and flavor. Accurate detection and quantification of D. segeticola growth in tea plant leaves are crucial for diagnosing disease severity or evaluating host resistance. In this study, we monitored disease progression and D. segeticola development in tea plant leaves inoculated with a GFP-expressing strain. By contrast, a DNA-based qRT-PCR analysis was employed for a more convenient and maneuverable detection of D. segeticola growth in tea leaves. This method was based on the comparison of D. segeticola-specific DNA encoding a Cys2His2-zinc-finger protein (NCBI accession number: OR987684) in relation to tea plant Cs18S rDNA1. Unlike ITS and TUB2 sequences, this specific DNA was only amplified in D. segeticola isolates, not in other tea plant pathogens. This assay is also applicable for detecting D. segeticola during interactions with various tea cultivars. Among the five cultivars tested, 'Zhongcha102' (ZC102) and 'Fuding-dabaicha' (FDDB) were more susceptible to D. segeticola compared with 'Longjing43' (LJ43), 'Zhongcha108' (ZC108), and 'Zhongcha302' (ZC302). Different D. segeticola isolates also exhibited varying levels of aggressiveness towards LJ43. In conclusion, the DNA-based qRT-PCR analysis is highly sensitive, convenient, and effective method for quantifying D. segeticola growth in tea plant. This technique can be used to diagnose the severity of tea leaf spot and blight or to evaluate tea plant resistance to this pathogen.

Biochemical Defence of Plants against Parasitic Nematodes.

Plant parasitic nematodes (PPNs), such as Meloidogyne spp., Heterodera spp. and Pratylenchus spp., are obligate parasites on a wide range of crops, causing significant agricultural production losses worldwide. These PPNs mainly feed on and within roots, impairing both the below-ground and the above-ground parts, resulting in reduced plant performance. Plants have developed a multi-component defence mechanism against diverse pathogens, including PPNs. Several natural molecules, ranging from cell wall components to secondary metabolites, have been found to protect plants from PPN attack by conferring nematode-specific resistance. Recent advances in omics analytical tools have encouraged researchers to shed light on nematode detection and the biochemical defence mechanisms of plants during nematode infection. Here, we discuss the recent progress on revealing the nematode-associated molecular patterns (NAMPs) and their receptors in plants. The biochemical defence responses of plants, comprising cell wall reinforcement; reactive oxygen species burst; receptor-like cytoplasmic kinases; mitogen-activated protein kinases; antioxidant activities; phytohormone biosynthesis and signalling; transcription factor activation; and the production of anti-PPN phytochemicals are also described. Finally, we also examine the role of epigenetics in regulating the transcriptional response to nematode attack. Understanding the plant defence mechanism against PPN attack is of paramount importance in developing new, effective and sustainable control strategies.

Overall burden and impact on health-related quality of life associated with intravesical treatment of patients with non-muscle invasive bladder cancer in the United States

Background This study aimed to describe the life impacts of intravesical therapies for non-muscle invasive bladder cancer (NMIBC) from a patient perspective. Methods A cross-sectional online survey design was used. Adults with NMIBC (and no other cancer) treated intravesically in the prior 12 months were recruited from US patient online communities. Individuals participating in a clinical trial or treated with erdafitinib were excluded. Participants’ treatment experiences were evaluated using a questionnaire comprising (a) custom questions reported on 11-point numerical rating scales and (b) validated patient reported outcome (PRO) measures for bladder symptom burden and work productivity. Results Among 171 survey participants, most received bacillus Calmette-Guérin (BCG) (83%), intravesical gemcitabine (28%), or gemcitabine + docetaxel (13%) during the past year. Participants generally felt adequately informed about treatment, felt expectation of treatment matched actual experience, and expressed intent to complete the full treatment course and willingness to try different treatments if needed. Participants reported disease symptom burden of 42.6/72 on the NFBlSI-18 scale. Employed participants reported 51% work impairment and 59% overall work productivity loss due to NMIBC. Conclusions Participants recently treated with intravesical therapies expressed intent to complete the full treatment course and willingness to try new therapies if needed. Participants reported high NMIBC symptom burden and work impairment negatively impacting their well-being, despite receiving intravesical treatment.

Multicentric Study on Enteric Protists Occurrence in Zoological Parks in Portugal.

Parasitic infections of the gastrointestinal tract of domestic animals play a major role in the transmission of disease, which in turn may result in financial and productive losses. Notwithstanding, studies on the burden and distribution of diarrheagenic protists in zoological gardens are still insufficient. Given the close animal-animal and animal-human interaction in these settings, Public Health concerns under the One Health context are raised. Using molecular detection tools and phylogenetic analysis, the goal of this study was to assess the occurrence of four potentially zoonotic protists-Balantioides coli, Blastocystis sp., Cryptosporidium spp. and Giardia spp.-in animals residing in zoological parks in Portugal. Occurrence of Eimeria spp. was also assessed because of its veterinary relevance. Although Blastocystis sp. represents most of the positive samples obtained (11.6%; 95% CI: 0.08-0.17), all parasites were detected (B. coli (2.9%; 95% CI: 0.01-0.06), and Cryptosporidium spp., Eimeria spp. and Giardia spp. presented the same prevalence (0.5%; 95% CI: 0.00-0.03)). We also describe the first molecular detection of B. coli in a collared peccary (Tayassu tajacu), of Blastocystis sp. in three different python species, and G. muris in a central bearded dragon (Pogona vitticeps), suggesting the broadening of the host range for these parasites.

A diagnostic gap to fill: Development of molecular tools to distinguish the cyst-forming nematode species Heterodera carotae and Heterodera cruciferae

The carrot cyst nematode, Heterodera carotae, is an important pest causing qualitative and quantitative production losses across several countries worldwide. In culture crops, Heterodera carotae and Heterodera cruciferae, can be found in mixture and are virtually unrecognizable from a morphological point of view. However, only H. carotae is able to develop on carrot. To monitor carrot production and set up suitable H. carotae control plans, simple and reliable diagnostic methods to detect and identify these two species are needed. In this study, we developed and successfully tested two real-time PCR protocols (SYBR Green singleplex and TaqMan multiplex real-time PCRs) against both species: H. carotae and H. cruciferae. Using two specific sets of primers, we managed, whatever the methods, to distinguish the two species. Moreover, we showed a higher specificity using the TaqMan assays but a better sensitivity with the SYBR Green assays. In this study, we are proposing two useful and reliable diagnostic tools to enable farmers and scientists to rapidly detect two morphologically and genetically closely related species and supervise agricultural management.