Two types of cuts were performed at a 4–5 week interval in the same ovariectomized rats; horizontal half-circle cut located just above the anterior commissure (ARD) and half-dome cut located anterior to the ventromedial nucleus (AD). Behavioral tests were carried out following the pretreatment with estradiol benzoate for 3 days and progesterone on the fourth day. When females received AD first (Experiment 1) the mean LQ was significantly lower than that of controls without brain surgery. Then, the AD rats were subjected to ARD or ARD sham. At the second test, the mean LQ of AD-ARD rats increased to the control level, but the LQ of AD-ARD sham rats was still low. In the experiment 2, the order of the brain surgery was just reversed. In the first test, all ARD females showed high levels of lordosis and the mean LQ was higher than that of control. Then, these ARD females receiving AD or AD sham were subjected to the second test. The mean LQ of ARD-AD rats decreased to the level of the control, but the LQ of ARD-AD sham rats was still high. Thus, dorsal neural inputs to or through the preoptic area and hypothalamus may exert inhibitory influences on a lordosis mediating system and anterolateral outputs of the medial basal hypothalamus appear to be concerned with a lordosis facilitating mechanism. These two systems seem to have a mutual correlation in regulating lordosis response. However, ARD or AD could not completely reverse the suppressive effect of AD or the facilitatory effect of ARD in the animals with dual cuts. It is suggested that the dorsal extrahypothalamic inhibitory influence and the hypothalamic facilitatory influence can regulate the display of lordosis independently in female rats.