To fully understand the tritium aging behavior of palladium (Pd), an important material applied in hydrogen isotope storage and tritium engineering, an in situ irradiation of 30 keV H2+ and He+ dual beam was performed for the investigation of the microstructural evolution in Pd. The irradiation dose, temperature, and sample thickness obviously affected the morphology, distribution characteristics, density, and size of dislocation loops, bubbles, and bubble-loop complexes. Highly dense loops were aggregating at lowered temperatures, whereas the loop density dropped dramatically, producing bubble-loop complexes at elevated temperatures. Most of loops were 1/3<111> Frank loops with quasi-circular under a low dose at 573 K and then transformed to 1/2<110> perfect loops with wavy and polygonal shape at a high dose. The difference in growth behavior between faulted and perfect loops suggested that there was a critical transform loop size ~32 nm in diameter. The heterogeneity in size and shape of bubbles could be attributed to the interaction between bubbles and the periphery loop. Moreover, the hydrogen resulted in the formation of larger bubbles when compared with the single-beam helium irradiation. The unfaulting transition of loops led to bubble redistribution. The corresponding mechanisms of in situ irradiation experimental results were discussed.