Rhinichthys Cataractae Research Articles

Identifying transcriptomic indicators of tertiary treated municipal effluent in longnose dace (Rhinichthys cataractae) caged under semi-controlled conditions in experimental raceways

To evaluate effects of tertiary treated wastewater treatment plant effluent (MWWE) on transcriptomic responses in longnose dace (Rhinichthys cataractae; LND) we conducted a semi-controlled study in experimental raceways (Advancing Canadian Water Assets facility) imbedded in the Pine Creek treatment plant (Calgary, AB). LND collected from a reference site in the Bow River (REF) were caged in raceways containing either 5 % Pine Creek effluent (PC) or Bow River water (BR; control) over 28 d. Liver transcriptomes were analyzed in males and females sampled on days 7, 14 and 28 from BR and PC, and compared to REF fish on day 0. Concurrent with the caging, selected environmental substances of concern were analyzed in the BR and PC. Significantly different unigenes (SDUs) in females (vs males) within both BR and PC raceways increased over time and compared to REF fish. Moreover, SDUs in females and males within the same treatment (i.e., BR, PC) showed a temporal increase as well as compared to REF fish. Time was the dominant factor affecting SDUs, whereas sex and treatment had less of an impact on the transcriptome profiling. Gene Set Enrichment Analysis of BR vs PC over time revealed effects on genes involved in growth, metabolism of carbohydrates and lipids, and immune system on day 7; however, by day 28, 80–100 % of the transcripts localized to enriched biomarkers were associated with tissue immune responses in both sexes. Exposure to 5 % effluent had significant effects on female liver somatic index but no effects were observed on other phenotypic health indices in either sex. BR was used as the source of reference water, but analyses showed trace amounts of ESOCs. Analyses did not point towards definitive response patterns that could be used in field-based ecotoxicogenomic studies on the impacts of well-treated MWWE but suggested compromised adaptive immune responses.

Effects of non-native Salmo trutta and multiscale habitat factors on native fishes in the Driftless Area

We collected fishes and habitat data at 138 streams to evaluate the effects of introduced brown trout ( Salmo trutta) and habitat conditions on occurrence, detection, abundance, and size structure of sculpin ( Cottus spp.), longnose dace ( Rhinichthys cataractae), and southern redbelly dace ( Chrosomus erythrogaster) in the Driftless Area, USA. Sculpin detection decreased with increasing stream velocity, whereas southern redbelly dace detection increased with stream depth. Sculpin occupancy declined with increasing stream temperature and velocity and increased with increasing forested land, boulder substrate, and brown trout length and abundance. Longnose and southern redbelly dace occupancy and abundance declined with increasing brown trout abundance and occupancy increased with stream temperature. Longnose dace occupancy also increased with increasing stream temperature and cobble substrate and declined with increasing elevation. Native fish size structure was unrelated to brown trout presence. Our results suggest that effects of brown trout are not ubiquitous across native fishes and depend on abiotic conditions and species-specific habitat requirements, highlighting the need to consider both biotic and abiotic conditions when balancing native species conservation with introduced sportfish management.

Nearctic Dactylogyrus species (Platyhelminthes, Monogenea) parasitizing cypriniform fishes in the context of morphology and phylogeny, with descriptions of seven new species.

Ribosomal DNA sequences are currently available for 32 morphologically recognized species of Dactylogyrus parasitizing Nearctic cypriniforms, but only 16 of them are assigned to nominal species. Herein, morphological data on 28 of the 32 species are provided, together with comments on their phylogenetic relationships in the context of the morphology of taxonomically important structures. Seven new species of Dactylogyrus are described from five species of leuciscids and one species of catostomids, as follows: D. aduncus n. sp. from Campostoma spadiceum, D. cloutmani n. sp. from Luxilus chrysocephalus isolepis, D. cornifrons n. sp. from Cyprinella venusta, D. fimbratus n. sp. from Rhinichthys cataractae, D. mcallisteri n. sp. from Semotilus atromaculatus, and D. chieni n. sp. and D. haneki n. sp. from Hypentelium nigricans. Four previously described species of Dactylogyrus, D. atromaculatus from S. atromaculatus, D. eos from C. neogaeus, D. parvicirrus from Notemigonus crysoleucas and D. perlus from L. c. isolepis, are redescribed and/or figured. As for the remaining 17 species, only the male copulatory organs (MCOs) taken from the respective hologenophores are illustrated. On the basis of phylogenetic analyses, two main clades of Nearctic Dactylogyrus were recognized and supported by the different morphology of the MCO. The first one included 22 strictly Nearctic species sharing the same MCO type with Dactylogyrus spp. parasitizing cyprinids likely of Asian origin. The second clade encompassed Dactylogyrus spp. with diverse MCO morphology and was placed in the sister position to Dactylogyrus spp. parasitizing European leuciscids and North-West African cyprinids.

Scale‐dependence of fish habitat associations in a North American river system

Abstract Determining the appropriate measurement scale to assess habitat variables is critical for ecologists assessing biological or ecological conditions. Depth, velocity, substrate, woody debris and other fish cover variables occur on both reach and microhabitat scales, and fish habitat associations with these variables may be scale‐dependent. The aim of this work was to better understand the importance of scale for fish–habitat associations with these variables in a framework consistent with environmental filtering and to test the hypothesis that habitat variable importance is scale‐dependent. I used prepositioned areal electrofishing in wadeable streams of the Delaware River basin to evaluate the associations of fish with the same variables summarised on different reach and microhabitat scales. The importance of scale for fish–habitat associations was assessed using two approaches that approximate an environmental filtering framework: variance partitioning with (1) ordination and (2) generalised linear mixed models. Variables on both the reach and microhabitat scales explained a significant fraction of the total variation in fish community composition (p < 0.05). Variation decomposition of reach‐ and microhabitat‐scale effects revealed 20.2% and 2.0% of all variation were due uniquely to reach and microhabitat scales, respectively. Measures of coarseness, embeddedness, amount of riffle and areal coverage of five fish cover variables were significant explanatory variables of community composition at the reach scale only (p < 0.05). Velocity and mesohabitat (amount or presence of riffle) were the only two habitat features that were significant explanatory variables of fish community composition at both the reach and microhabitat scales (p < 0.05). Individual models of species occurrence revealed similar patterns as seen with analyses of community composition. For many fishes, habitat features quantified at the reach scale were more explanatory than at the microhabitat scale. Longnose dace (Rhinichthys cataractae) were more dependent upon microhabitat variables than reach‐scale variables, relative to other fishes. Mean velocity at the reach scale was the most important explanatory variable for explaining fish community composition and indicated support for the concept of environmental filtering at the reach and microhabitat scales. Few studies of fish occurrence have incorporated a study design and analytical framework that approximates the hierarchical nature of habitat. This study identifies important scales and predictors, demonstrates the importance of a multiscale approach, and provides support for the environmental filtering concept at the reach and microhabitat scales. These findings will allow ecologists to better account for scale‐dependent habitat associations and justify the use of fish habitat associations on reach and microhabitat scales for assessing biotic integrity, restoration and conservation of fishes.

Spatial patterns of stable isotopes and trophic ecology in a hydropeaking river

AbstractWhile the impacts of dams are known, there are gaps in our understanding, particularly with respect to longitudinal patterns of environmental and biological gradients downstream of dams. We investigated longitudinal patterns of stable isotope ratios of carbon and nitrogen in brook trout (Salvelinus fontinalis) in the regulated Magpie River. We also examined the diet of brook trout via mixing models using the dominant forage items in the river. For brook trout, δ13C values increased and δ15N values decreased in reaches progressing downstream. River distance downstream of the dam explained the variation in levels of stable isotopes in brook trout. Most changes occurred within the first eight kilometres of the river. The fish length had a significant but weak positive effect on stable isotope levels. Based on the MixSiar mixing model, the diet of brook trout consisted of 72% longnose dace, 27% benthic invertebrates, and 1% sculpin. The Magpie River had isotope values that were lower for δ13C and higher for δ15N when compared to nine neighbouring natural unregulated rivers. Previous research on the Magpie River demonstrated that the upstream reservoir exports large quantities of plankton that likely provides a quality food source for river biota and shows a clear downstream gradient in energy sources similar to the changes found for δ13C. For the Magpie River, and other regulated rivers, statistical comparisons of many abiotic and biotic variables, including stable isotopes, could lead to erroneous conclusions if samples are not collected with explicit consideration of spatial gradients.

Influence of beaver mimicry restoration on habitat availability for fishes, including Arctic grayling (Thymallus arcticus)

AbstractBeaver dam mimicry is an emergent conservation practice. We evaluated the influence of constructed riffles, a unique type of beaver mimicry aimed to store water and allow fish passage, on habitat for fishes in one control reach and one manipulated reach with mimicry structures added. The beaver mimicry reach had deeper pool habitats and deeper and wider riffle habitats compared to an unmanipulated control reach. Dissolved oxygen was similar among reaches, averaging 8.7 ± 0.2 and 8.9 mg/L in the beaver mimicry and control reaches, respectively. Sediment size was also similar among reaches, with a D50 of 8.1 and 10.6 mm in the beaver mimicry and control reaches, respectively. The beaver mimicry reach had little to no overhanging bank vegetation or riparian vegetation shade cover, while the control had 38% of its bank covered by canopy and 56% overhung by vegetation. These riparian characteristics result from a legacy of livestock grazing and lack of consistent vegetation planting during restoration. Longnose dace (Rhinichthys cataractae) and white sucker (Catostomus commersonii) dominated in the beaver mimicry reach, together comprising 70% of the fish assemblage post‐structure installation. Arctic grayling (Thymallus arcticus) was not found in the beaver mimicry reach but was present in the control, albeit in small numbers of only 3% of the assemblage post‐structure installation. These results highlight the need to consider both in‐stream and riparian habitat features for fishes, as well as timescales of both hydrological and ecological outcomes in restoration design.

Diets and Stable Isotope Signatures of Native and Nonnative Leucisid Fishes Advances Our Understanding of the Yellowstone Lake Food Web

(1) Many forage fishes, such as Leucisids (minnows) have depauperate studies on diet composition or stable isotope signatures, as these fishes are often only viewed as food for higher trophic levels. The need exists to understand and document the diet and stable isotope signatures of Leucisids (redside shiner, longnose dace, lake chub) in relation to the community ecology and food-web dynamics in Yellowstone Lake, especially given an invasive piscivore introduction and potential future effects of climate change on the Yellowstone Lake ecosystem. (2) Diet data collected during summer of 2020 were analyzed by species using proportion by number, frequency of occurrence, and mean proportion by weight, and diet overlap was compared using Schoener’s index (D). Stable isotope (δ15N and δ13C) values were estimated by collecting tissue during the summer of 2020 by species, and isotopic overlap was compared using 40% Bayesian ellipses. (3) Nonnative redside shiners and lake chub had similar diets, and native longnose dace diet differed from the nonnative Leucisids. Diet overlap was also higher between the nonnative Leucisids, and insignificant when comparing native and nonnative Leucisids. No evidence existed to suggest a difference in δ15N signatures among the species. Longnose dace had a mean δ13C signature of −15.65, indicating an decreased reliance on pelagic prey compared to nonnative Leucisids. Nonnative redside shiners and lake chub shared 95% of isotopic niche space, but stable isotope overlap was <25% for comparisons between native longnose dace and the nonnative Leucisids. (4) This study established the diet composition and stable isotope signatures of Leusicids residing in Yellowstone Lake, thus expanding our knowledge of Leucisid feeding patterns and ecology in relation to the native and nonnative species in the ecosystem. We also expand upon our knowledge of Leucisids in North America. Additionally, quantifying minnow diets can provide a baseline for understanding food web response to invasive suppression management actions.

Nonhost species reduce parasite infection in a focal host species within experimental fish communities.

The dilution effect describes the negative association between host biodiversity and the risk of infectious disease. Tests designed to understand the relative roles of host species richness, host species identity, and rates of exposure within experimental host communities would help resolve ongoing contention regarding the importance and generality of dilution effects. We exposed fathead minnows to infective larvae of the trematode, Ornithodiplostomum ptychocheilus in minnow‐only containers and in mixed containers that held 1–3 other species of fish. Parasite infection was estimated as the number of encysted worms (i.e., brainworms) present in minnows following exposure. The results of exposure trials showed that nonminnow fish species were incompatible with O. ptychocheilus larvae. There was no reduction in mean brainworm counts in minnows in mixed containers with brook sticklebacks or longnose dace. In contrast, brainworm counts in minnows declined by 51% and 27% in mesocosms and aquaria, respectively, when they co‐occurred with emerald shiners. Dilution within minnow + shiner containers may arise from shiner‐induced alterations in minnow or parasite behaviors that reduced encounter rates between minnows and parasite larvae. Alternatively, shiners may act as parasite sinks for parasite larvae. These results highlight the role of host species identity in the dilution effect. Our results also emphasize the complex and idiosyncratic effects of host community composition on rates of parasite infection within contemporary host communities that contain combinations of introduced and native species.

Wild longnose dace downstream of wastewater treatment plants display an obese phenotype

Wild fish living downstream of wastewater treatment plants (WWTPs) often have increased body condition factors or body mass indices compared to upstream fish. This observation has been largely attributed to increased nutrient loading and food availability around wastewater effluent outflows. While a higher condition factor in fish is generally considered a predictor of healthy ecosystems, the metabolic status and capacity of the animals downstream of WWTPs may be a better predictor of fitness and potential population level effects. To address this, we sampled wild longnose dace (Rhinichthys cataractae), a native species in North American waterways, from sites upstream and downstream of WWTPs. Downstream fish had higher body mass indices, which corresponded with higher nutrient (lipid, protein, and glycogen) storage in somatic tissues compared to upstream fish. Liver transcriptome analysis revealed metabolic reprogramming favoring lipid synthesis, including higher hepatic triglyceride levels and transcript abundance of targeted lipogenic genes. This suggests that effluent exposure-mediated obesity in dace is a result of changes at the transcriptional level. To determine potential ecological consequences, we subjected these fish to an acute stressor in situ to determine their stress performance. Downstream fish failed to mobilize metabolites post-stress, and showed a reduction in liver aerobic and anaerobic metabolic capacity. Taken together, fish living downstream of WWTPs exhibit a greater lipid accumulation that results in metabolic disruption and may compromise the ability of these fish to cope with subsequent environmental and/or anthropogenic stressors.

Multispecies Fish Passage Evaluation at a Rock‐Ramp Fishway in a Colorado Transition Zone Stream

AbstractStream habitat fragmentation caused by manmade structures is ubiquitous in Colorado, creating a need for passage solutions that accommodate multiple fish species. This study tested the effectiveness of a rock‐ramp fishway for passing nine fish species with a range of swimming abilities. The target species for the fishway design included Brassy MinnowHybognathus hankinsoni(weakest swimming), Longnose DaceRhinichthys cataractae, Longnose SuckerCatostomus catostomus, and Brown TroutSalmo trutta(strongest swimming). Testing included a 46‐h enclosure study and 3‐month extended study, during which fish passage was evaluated using PIT tags. All of the species exhibited successful passage through the fishway during the enclosure study, but movement probabilities varied by species. Five species were not detected at the fishway during the extended study, possibly due to issues with attraction flows, entrance conditions, or motivation. Hydraulic conditions within the fishway were also evaluated. Roughness elements maintained a benthic, low‐velocity zone across a range of flows, even when surface and depth‐averaged velocities surpassed the design criteria for the weakest swimming species. The methods from this study could be replicated at other locations to evaluate design criteria (e.g., slope, capacity, roughness, and configuration) and performance for a variety of fish species and fishway types.

Landscape resistance mediates native fish species distribution shifts and vulnerability to climate change in riverscapes.

A broader understanding of how landscape resistance influences climate change vulnerability for many species is needed, as is an understanding of how barriers to dispersal may impact vulnerability. Freshwater biodiversity is at particular risk, but previous studies have focused on popular cold-water fishes (e.g., salmon, trout, and char) with relatively large body sizes and mobility. Those fishes may be able to track habitat change more adeptly than less mobile species. Smaller, less mobile fishes are rarely represented in studies demonstrating effects of climate change, but depending on their thermal tolerance, they may be particularly vulnerable to environmental change. By revisiting 280 sites over a 20year interval throughout a warming riverscape, we described changes in occupancy (i.e., site extirpation and colonization probabilities) and assessed the environmental conditions associated with those changes for four fishes spanning a range of body sizes, thermal and habitat preferences. Two larger-bodied trout species exhibited small changes in site occupancy, with bull trout experiencing a 9.2% (95% CI=8.3%-10.1%) reduction, mostly in warmer stream reaches, and westslope cutthroat trout experiencing a nonsignificant 1% increase. The small-bodied cool water slimy sculpin was originally distributed broadly throughout the network and experienced a 48.0% (95% CI=42.0%-54.0%) reduction in site occupancy with declines common in warmer stream reaches and areas subject to wildfire disturbances. The small-bodied comparatively warmer water longnose dace primarily occupied larger streams and increased its occurrence in the lower portions of connected tributaries during the study period. Distribution shifts for sculpin and dace were significantly constrained by barriers, which included anthropogenic water diversions, natural step-pools and cascades in steeper upstream reaches. Our results suggest that aquatic communities exhibit a range of responses to climate change, and that improving passage and fluvial connectivity will be important climate adaptation tactics for conserving aquatic biodiversity.

Effects of Substrate on Movement Patterns and Behavior of Stream Fish through Culverts: An Experimental Approach

Culverts can provide a significant barrier to fish passage by fragmenting fish habitats and impeding the passage success of small-bodied fish. Geographical connectivity is critical to the maintenance of diverse fish assemblages. Culverts with high cross-sectional velocity can cause population fragmentation by impeding passage of small, freshwater fish. Behavioral responses of small fish to high velocities can differ among functional groups, and swimming behavior of many species is not well known. We tested effects of substrate type on swimming behavior in two small, freshwater fish species—southern leatherside chub (Lepidomeda aliciae, a midwater species), and longnose dace (Rhinichthys cataractae, a benthic species)—across three substrate treatments: (1) a bare flume, (2) large flow obstacles, and (3) a natural cobble substrate. Both longnose dace and southern leatherside chub used paths of low velocity and swam in the near-substrate boundary area. Fish in the bare flume and large obstacle treatments swam along the corners of the flume in a straight swim path, whereas fish in the natural substrate treatment used all parts of the flume bed. There was no relationship between passage success of fish and substrate type, fish species, or their interaction. In contrast, substrate type, fish species, and their interaction were significant predictors of passage time. Southern leatherside chub passed through the test section about two to four times faster than longnose dace. Both species took longer to pass through the large flow obstacle treatment compared to the bare flume or natural substrate. The natural substrate created a complex velocity profile with areas of low velocity throughout the entire flume, in contrast to the other two treatments. Our data suggest natural substrates can improve the passage of small fish in high-velocity culverts for both benthic and midwater functional groups.

Spatial genetic variation and habitat association of Rhinichthys cataractae, the longnose dace, in the Driftless Area of the upper Mississippi River basin

The Driftless Area of the upper Mississippi River drainage is a unique geographic region because of its complex geological history and the influence of recent, intensive human activities. The longnose dace, Rhinichthys cataractae, is a relatively common, small freshwater fish that is distributed in swift, cool streams within the region. The aim of this study was to determine the spatial genetic differentiation of the longnose dace and define the broad scale environmental variables that shape the distribution of the species in the southwestern portion of the Driftless Area. Genotypic data from seven microsatellite loci were analyzed for 276 individuals from 15 localities representing major drainages within the region in northeast Iowa. Broad scale environmental variables including hydrologic, soil, and climatic factors were evaluated to construct an ecological niche model (ENM) to predict the suitability of habitat for the species within the region. Results of the genetic analyses revealed two distinct, but somewhat admixed genetic clusters of longnose dace in Iowa. The genetic differentiation between localities and between drainages was low to moderate with some evidence of isolation by distance. Most of the variation was observed by differences between individuals within local populations. The ENM generated largely reflected the known distribution of the species in Iowa with a decreasing probability of suitable habitat from northern to southern drainages. Geologic factors played a key role in the model. The distribution and population structure of the longnose dace in the northeast Iowa revealed that isolation by distance, historical processes and the underlying geology are primarily responsible for the observed spatial distribution of genetic variation.

The evolution of the major histocompatibility complex in upstream versus downstream river populations of the longnose dace.

Populations in upstream versus downstream river locations can be exposed to vastly different environmental and ecological conditions and can thus harbor different genetic resources due to selection and neutral processes. An interesting question is how upstream–downstream directionality in rivers affects the evolution of immune response genes. We used next‐generation amplicon sequencing to identify eight alleles of the major histocompatibility complex (MHC) class II β exon 2 in the cyprinid longnose dace (Rhinichthys cataractae) from three rivers in Alberta, upstream and downstream of municipal and agricultural areas along contaminant gradients. We used these data to test for directional and balancing selection on the MHC. We also genotyped microsatellite loci to examine neutral population processes in this system. We found evidence for balancing selection on the MHC in the form of increased nonsynonymous variation relative to neutral expectations, and selection occurred at more amino acid residues upstream than downstream in two rivers. We found this pattern despite no population structure or isolation by distance, based on microsatellite data, at these sites. Overall, our results suggest that MHC evolution is driven by upstream–downstream directionality in fish inhabiting this system.

Distribution and Abundance of Millicoma Dace In the Coos River Basin, Oregon

Abstract The Millicoma Dace Rhinichthys cataractae is a form of Longnose Dace endemic to the Coos River drainage in southwestern Oregon. Sparse species records in the Oregon State University Ichthyology Collection and database and infrequent recent encounters prompted surveys to assess the current status and distribution of the species. In 2014, we surveyed locations that had historically supported Millicoma Dace using backpack electrofishing to describe their current distribution and abundance at these locations. In 2015, we extended these surveys further upstream in the South Coos River basin, outside of the documented historical range. We used an N-mixture model to estimate abundance and capture probability for Millicoma Dace at each sampling location. We evaluated the effects of habitat covariates on both capture probability and abundance at each sample site. We found Millicoma Dace were widespread throughout their historical range and in the South Coos River sites outside of their documented historic...

Evaluation of swimming performance for fish passage of longnose dace Rhinichthys cataractae using an experimental flume.

The swimming performance of longnose dace Rhinichthys cataractae, the most widely distributed minnow (Cyprinidae) in North America, was assessed in relation to potential passage barriers. The study estimated passage success, maximum ascent distances and maximum sprint speed in an open-channel flume over a range of water velocities and temperatures (10·7, 15·3 and 19·3° C). Rhinichthys cataractae had high passage success (95%) in a 9·2 m flume section at mean test velocities of 39 and 64 cm s-1 , but success rate dropped to 66% at 78 cm s-1 . Only 20% of fish were able to ascend a 2·7 m section with a mean velocity of 122 cm s-1 . Rhinichthys cataractae actively selected low-velocity pathways located along the bottom and corners of the flume at all test velocities and adopted position-holding behaviour at higher water velocities. Mean volitional sprint speed was 174 cm s-1 when fish volitionally sprinted in areas of high water velocities. Swimming performance generally increased with water temperature and fish length. Based on these results, fishways with mean velocities <64 cm s-1 should allow passage of most R. cataractae. Water velocities >100 cm s-1 within structures should be limited to short distance (<1 m) and structures with velocities ≥158 cm s-1 would probably represent movement barriers. Study results highlighted the advantages of evaluating a multitude of swimming performance metrics in an open-channel flume, which can simulate the hydraulic features of fishways and allow for behavioural observations that can facilitate the design of effective passage structures.

The Intestinal Parasites of an Assemblage of Stream Fishes in Central Utah

Abstract. Parasite communities of stream fish assemblages in the western United States are poorly known. We investigated the intestinal parasites of an assemblage of stream fishes in Spanish Fork River in central Utah, USA. Two species, mottled sculpin (Cottus bairdii) and redside shiner (Richardsonius balteatus), had no observable parasites. In the other 3 species, southern leatherside chub (Lepidomeda aliciae), mountain sucker (Catostomus platyrhynchus), and longnose dace (Rhinichthys cataractae), we identified metacercia of an unidentified trematode. We found adult cestodes in southern leatherside chub only. Prevalence and intensity of parasites was highest in southern leatherside chub. Reasons for differential prevalence and intensity of parasites among host fish species are unknown, but there seems to be no relationship to trophic position or habitat use in this fish community.

Growth, Condition and Survival of Three Forage Fish Species Exposed to Two Different Experimental Hydropeaking Regimes in a Regulated River

AbstractHydroelectric dam operation can alter discharge and temperature patterns, impacting fish populations downstream. Previous investigations into the effects of river regulation on fish have focused on a single species within a river, yet different results among studies suggest the potential for species‐specific impacts. Here, we compare the impacts of two different hydropeaking regimes relative to a naturally flowing river on three ecologically important members of the forage fish community: longnose dace (Rhinichthys cataractae), slimy sculpin (Cottus cognatus) and trout‐perch (Percopsis omiscomaycus). Annual growth, estimated from otolith back‐calculations, was higher for each of the species in the regulated river relative to the naturally flowing river but did not differ between hydropeaking regimes. Condition was assessed using weight–length relationships and differed between rivers for each species, and between hydropeaking regimes for longnose dace and slimy sculpin. Survival of longnose dace and slimy sculpin was lower in the regulated river relative to the naturally flowing river, but comparable between rivers for trout‐perch. Annual growth was significantly related to mean summer discharge in the regulated river and to mean summer water temperature in the naturally flowing river for each species, and significantly different slopes among species indicate species‐specific responses to discharge and temperature alterations. This study demonstrates different biological responses among fish species within rivers to regulation in general, as well as to specific hydropeaking regimes. Future studies should focus on multiple species and multiple indicators of fish health to more fully characterize the impacts of river regulation on downstream fish communities. Copyright © 2016 John Wiley &amp; Sons, Ltd.

Detoxification Efforts in Longnose Dace (&amp;lt;i&amp;gt;Rhinichthys cataractae&amp;lt;/i&amp;gt;) Exposed to Municipal and Agricultural Inputs

Ecological impacts of contaminants on population patterns in wild fish are impacted by many contaminants that readily enter aquatic systems. Responses to toxicants by individuals in lab studies generally do not predict population level consequences in natural systems. Trace levels of contaminants are present in all major rivers in southern Alberta, Canada, with concentrations higher down-stream of anthropogenic inputs like agricultural land-use and inputs of municipal wastewater effluents. Longnose dace (Rhinichthys cataractae) were used as a sentinel species to study field-based population-level responses to contaminants. We hypothesized that biomarker activity, triggered by contaminant exposure, should increase downstream of anthropogenic inputs in two southern Alberta rivers, with corresponding relations between biomarker activity and sex ratios, after accounting for age structure. Liver detoxification (ethoxyresorufin-O-deethylase activity = EROD) measured at reference and exposed sites on each river differed significantly in only the Bow River system. Sex ratios varied more downstream of anthropogenic inputs than upstream, but the direction of sex ratio bias was inconsistent and temporally dynamic. Sex ratios correlated with liver detoxification in only the Bow River. Taken together, these results suggest that contaminants alter sex ratios in long-nose dace, but that there is variation in anthropogenic stressors among rivers.

Phylogeography of the longnose dace (Rhinichthys cataractae) species group in northwestern North America — the Nooksack dace problem

The longnose dace (Rhinichthys cataractae (Valenciennes, 1842); Cyprinidae) is one of the most widespread freshwater fishes in North America, and across its range there have been several divergent forms described that are of uncertain taxonomic status. One of these forms, the Nooksack dace, is found in southwestern British Columbia and adjacent portions of western Washington, and is distinguished from longnose dace by a lower number of lateral-line scales. We sequenced a total of approximately 1400 base pairs (bp) of mitochondrial DNA (mtDNA) and noted that the longnose dace found west of the Continental Divide and Nooksack dace constituted reciprocally monophyletic clades that differed from each other by between 2% and 3% sequence divergence. Sequence analysis at two nuclear loci (the S7 ribosomal protein intron 1 (S7) and recombination activation gene 1 (RAG1)), however, showed no consistent difference between longnose dace and Nooksack dace and several alleles were shared between them. By contrast, consistent differences at both mtDNA and nuclear DNA loci were resolved between R. cataractae samples from east and west of the Continental Divide. The Nooksack dace does not appear to warrant separate taxonomic status from the longnose dace, but the mtDNA differences support its recognition as an important component of the evolutionary and biogeographic legacy of R. cataractae.