Long-term Health Effects Research Articles

Research Progress on the Relationship Between Artificial Sweeteners and Breast Cancer

Artificial sweeteners, as low-calorie sugar substitutes, have attracted much attention in recent years, especially in terms of their potential health effects. Although they add almost no calories, studies have shown that artificial sweeteners may affect metabolism by stimulating insulin secretion and changing the intestinal microbiota, increasing the risk of metabolic syndrome and type 2 diabetes. Breast cancer, as the most common cancer in the world, is related to multiple factors such as genetics and hormone levels. The results of studies on artificial sweeteners and breast cancer risk are conflicting, with some showing a positive correlation between the two and others failing to confirm it. Differences in study design, participant characteristics, and the types of sweeteners have led to this ambiguity. Although some studies have focused on mechanisms such as hormone disorders, insulin response, and changes in the intestinal microbiota, further exploration is needed to establish a causal relationship. Our review aims to comprehensively analyze the potential association between artificial sweeteners and breast cancer and its mechanisms, as well as encourage future studies to reveal its long-term health effects.

Spatial heterogeneity and predictors of stunting among under five children in Mozambique: a geographically weighted regression

IntroductionChildhood stunting, a major public health concern in many developing countries, is associated with impaired physical and cognitive development, increased risk of infectious diseases, and long-term adverse health and economic consequences. Mozambique is among the countries with the highest stunting rates in sub-Saharan Africa. This study aims to examine the spatial variation and identify the predictors of stunting among children under the age of five years in Mozambique.MethodsWe utilized children’s recode data and geographic coordinates extracted from the 2022–23 Mozambique Demographic and Health Survey dataset. A stratified two-stage cluster sampling approach was employed. The study included a total weighted sample of 3,910 children under the age of five years. A geographically weighted regression was used to identify predictors of stunting.ResultsThe prevalence of stunting among children under the age of five in Mozambique was 31.26% (95% CI: 29.88 to 32.65%). The Nampula (46.00%), Cabo Delgado (43.79%), Manica (40.88%), Zambezia (38.27%), Niassa (35.52%), and Tete (34.85%) regions of Mozambique were identified as hotspot areas of stunting. Several factors were significantly associated with the spatial heterogeneity in stunting, where household wealth index (poor and middle categories) and Mother’s age (15–19 years) were positively associated with higher occurrence of stunting, while having an employed mother and being a child under 2 years of age were negatively associated with stunting occurrence.ConclusionThe spatial heterogeneity in stunting patterns highlighted by this analysis suggests that a one-size-fits-all approach to address child undernutrition in Mozambique may not be effective. Instead, tailored, location-specific strategies that account for the predictors of stunting are necessary to effectively combat this persistent public health challenge. Policymakers and program implementers should prioritize the hotspot regions for targeted interventions, while also maintaining and strengthening the factors contributing to the lower stunting prevalence in the cold spot areas.

Penerapan Standar Keamanan pada Proses Pengelasan Industri Skala Kecil di Desa Balak Kecamatan Songgon

This article discusses safety regulations during the welding process to reduce the risk of workplace accidents, long-term health consequences, and improve the quality of finished products. The study examines various international safety regulations, practical safety measures, and incidents involving welding. An important first step in increasing employee awareness and understanding of the potential risks that may arise during the welding process is safety socialization. Risks associated with welding include exposure to UV light, hazardous gases, sparks and high temperatures, which, if not managed appropriately, can jeopardize worker health and safety. Therefore, to ensure the best possible implementation of occupational safety standards, organized teaching initiatives through outreach are required. Personal protective equipment (PPE), safe work practices, handling of dangerous goods, risk mitigation and emergency response training are examples of socialization activities. A thorough understanding of welding safety is believed to reduce the number of workplace accidents, increase output, and make the workplace safer. This community service focuses on conducting welding and work safety training for home welding workshop businesses in Balak Village, Songgon District, Banyuwangi Regency. The application of safety standards to the welding process aims to evaluate the effectiveness of welding safety socialization in improving workers' compliance with safety procedures, while providing recommendations for optimizing future training programs.

Biophysics of ACL Injuries

Anterior Cruciate Ligament (ACL) injuries rank among the most prevalent and severe types of injuries, significantly impacting both athletes and non-athletes alike. These injuries not only result in immediate physical impairment, such as intense pain, substantial swelling, and a marked loss of mobility, but also carry long-term health consequences that can alter a person’s quality of life. Chronic pain, persistent instability, and an increased risk of developing osteoarthritis are among the lasting effects that can follow an ACL injury. An in-depth understanding of the biophysics behind ACL injuries is paramount for devising effective prevention and treatment protocols. Biophysics, which combines principles from physics with biological systems, provides crucial insights into the mechanical and structural integrity of the ACL and its susceptibility to injury under various conditions. This systematic review aims to collate and synthesize the current knowledge surrounding the biophysical mechanisms that underlie ACL injuries.

Long-term employment and health effects of active labor market programs

Active labor market programs (ALMPs) are widely used to speed up return to work among the unemployed. We examine their long-run effects on employment- and health-related outcomes for different target groups, arguing that ALMPs are associated with heterogeneous effects for different target groups and may even detrimentally influence the mental health for the most disadvantaged groups. To this end, we use evidence from randomized controlled trials conducted in Denmark in 2005-2008, in which treatment groups were exposed to intensified active labor market policies in the form of more frequent compulsory meetings with case workers and/or early activation and estimate effects over a period of 10 y. In line with expectations, we find that while ALMPs have the potential to increase labor market participation among resourceful clients even 10 after the original intervention, they have long-run negative effects on the mental health for the most disadvantaged groups among the unemployed. The negative effects are entirely driven by clients who already prior to the trial had mental health issues. These findings suggest that the effects of ALMPs are lasting, but at the same time greatly depend on how they fit with the resources of clients.

Early-Life Parental Affection, Social Relationships in Adulthood, and Later-Life Cognitive Function.

Although research has demonstrated the long-term health consequences of childhood adversities, less is known about the long-term impact of positive childhood experiences, such as parental affection. Using longitudinal data (1995-2014) from the Midlife in the United States (MIDUS) study, we analyze structural equation models estimating direct and indirect pathways from early-life parental affection to changes in later-life cognitive function through relationship quality in adulthood among Black and White older adults (N = 1983). Analyses revealed significant indirect effects of parental affection on better cognitive function through higher levels of social support (both average social support and family social support) in adulthood in the full sample and among Black respondents. Indirect pathways through relationship strain and through friend support were not significant. This work elevates the importance of promoting positive parental relationships during childhood, with implications for better social relationships in adulthood and cognitive function in later life.

Beat the heat: wearable-based study of perceived heat stress and physiological strain in swiss track workers in a controlled climate chamber

Increasing temperatures pose new challenges for track workers (TWs), who endure prolonged exposure to extreme heat and humidity. New methods are critically needed to assess their performance and heat tolerance, aiming to mitigate workplace accidents and long-term health consequences. This study aimed to investigate the physiological effects of heat exposure on TWs, using wearable sensors to monitor key physiological parameters under controlled environmental conditions. Nineteen TWs participated in the study, which included two experimental sessions simulating different thermal environments: a typical Swiss summer night and a hot summer day. Participants' core body temperature, heart rate (HR), and skin temperature were monitored using wearable sensors, and physiological indexes were computed. In addition, perceptual strain index (PeSI) and psychomotor vigilance task (PVT) response times were recorded. Statistically significant increases in physiological parameters were observed under hotter conditions. The study identified statistically significant correlations between the PeSI and the physiological strain index and between PeSI and HR. Perceptual scores were consistently higher than the values derived from physiological measurements, suggesting a greater subjective experience of heat strain. The PVT response times were higher on the hotter day, reflecting increased cognitive strain due to heat exposure. The study highlights the critical impact of heat stress on TWs, with statistically significant increases in physiological and cognitive strain under higher temperatures. Future research should focus on real-world applications of heat strain monitoring.

Associations of long-term nitrogen dioxide exposure with a wide spectrum of diseases: a prospective cohort study of 0·5 million Chinese adults

Little evidence is available on the long-term health effects of nitrogen dioxide (NO2) in low-income and middle-income populations. We investigated the associations of long-term NO2 exposure with the incidence of a wide spectrum of disease outcomes, based on data from the China Kadoorie Biobank. This prospective cohort study involved 512 724 Chinese adults aged 30-79 years recruited from ten areas of China during 2004-08. Time-varying Cox regression models yielded adjusted hazard ratios (HRs) for the associations of long-term NO2 exposure with aggregated disease incidence endpoints classified by 14 ICD-10 chapters, and incidences of 12 specific diseases selected from three key ICD-10 chapters (cardiovascular, respiratory, and musculoskeletal diseases) found to be robustly associated with NO2 in the analyses of aggregated endpoints. All models were stratified by age-at-risk (in 1-year scale), study area, and sex, and were adjusted for education, household income, smoking status, alcohol intake, cooking fuel type, heating fuel type, self-reported health status, BMI, physical activity level, temperature, and relative humidity. The analysis of 512 709 participants (mean baseline age 52·0 years [SD 10·7]; 59·0% female and 41·0% male) included approximately 6·5 million person-years of follow-up. Between 5285 and 144 852 incident events were recorded for each of the 14 aggregated endpoints. Each 10 μg/m3 higher annual average NO2 exposure was associated with higher risks of chapter-specific endpoints, especially cardiovascular (n=144 852; HR 1·04 [95% CI 1·02-1·05]), respiratory (n=73 232; 1·03 [1·01-1·05]), musculoskeletal (n=54 409; 1·11 [1·09-1·14]), and mental and behavioural (n=5361; 1·12 [1·05-1·21]) disorders. Further in-depth analyses on specific diseases found significant positive supra-linear associations with hypertensive disease (1·08 [1·05-1·11]), lower respiratory tract infection (1·03 [1·01-1·06]), arthrosis (1·15 [1·09-1·21]), intervertebral disc disorders (1·13 [1·09-1·17]), and spondylopathies (1·05 [1·01-1·10]), and linear associations with ischaemic heart disease (1·03 [1·00-1·05]), ischaemic stroke (1·08 [1·06-1·11]), and asthma (1·15 [1·04-1·27]), whereas intracerebral haemorrhage (1·00 [0·95-1·06]), other cerebrovascular disease (0·98 [0·96-1·01]), acute upper respiratory infection (1·03 [0·96-1·09]), and chronic lower respiratory disease (0·98 [0·95-1·02]) showed no significant association. NO2 exposure showed robust null association with external causes (n=32 907; 0·98 [0·95-1·02]) as a negative control. In China, long-term NO2 exposure was associated with a range of diseases, particularly cardiovascular, respiratory, and musculoskeletal diseases. These associations underscore the pressing need to implement the recently tightened WHO air quality guidelines. Wellcome Trust, UK Medical Research Council, Cancer Research UK, British Heart Foundation, National Natural Science Foundation of China, National Key Research and Development Program of China, Sino-British Fellowship Trust, and Kadoorie Charitable Foundation.

Sleep Treatment Education Program for Cancer Survivors: Protocol for an Efficacy Trial.

Cancer survivors are at increased risk for chronic insomnia, even years after treatment completion. As insomnia is associated with a variety of long-term health consequences, access to insomnia treatment is critically important for the survivor population. Cognitive behavioral therapy for insomnia (CBTI) is the recommended first-line treatment for insomnia but remains largely unavailable to survivors. Treatment barriers include geographic limitations, a shortage of trained providers, and demanding treatment regimens. Designed with these limitations in mind, the Sleep Treatment Education Program (STEP-1) delivers components of CBTI in a low-intensity educational intervention delivered online. This is a phase II pilot randomized controlled trial. The primary aims are to test the efficacy of STEP-1 to improve (1) insomnia symptoms and (2) mood in cancer survivors compared to a control condition. The secondary aims will (1) explore participant factors associated with clinically significant response, (2) evaluate acceptability of the control intervention, (3) explore feasibility of delivering individualized coaching sessions for participants who do not have a significant response to STEP-1, and (4) describe participants' satisfaction with STEP-1 and suggestions for improvement. This 2-arm randomized controlled trial enrolled 70 off-treatment cancer survivors aged 40-89 years with clinically significant insomnia. Participants are randomized to receive either the STEP-1 intervention or control condition (relaxation education); interventions are delivered in one-on-one, synchronous, virtual videoconference sessions by trained interventionists. The STEP-1 intervention presents educational information on the development of insomnia after cancer and offers suggestions for improving insomnia symptoms based on the CBTI elements of sleep hygiene, stimulus control, and cognitive restructuring. With the interventionist, participants review the suggestions and develop a personalized sleep action plan for implementation. The relaxation education session provides information on the potential benefits of relaxation and how to independently access online relaxation exercises. The Insomnia Severity Index is used to measure insomnia symptoms, and the Profile of Mood States Short Form is used to measure mood at baseline and 4 and 8 weeks after intervention. The primary end point is change in the Insomnia Severity Index score at 8 weeks, and the secondary end point is change in mood symptoms (Profile of Mood States Short Form) at 8 weeks. This trial was funded in July 2022. Enrollment and data collection began in February 2023 and concluded in October 2024, with 70 participants enrolled. The analysis will begin in fall 2024, and the results are expected in winter 2025. Trial results will determine if STEP-1 effects go beyond those that could be attributed to placebo and other nonspecific treatment factors. Should results support the efficacy of STEP-1 to improve mood and insomnia symptoms, we anticipate developing efficacy and implementation trials of STEP-1 in larger and more diverse samples. ClinicalTrials.gov NCT05519982; https://clinicaltrials.gov/study/NCT05519982. DERR1-10.2196/60762.

Long-Term Health Effects of COVID-19: Disability and Function Following SARS-CoV-2 Infection and A Long COVID Definition: A Chronic, Systemic Disease State with Profound Consequences.

Long-Term Health Effects of COVID-19: Disability and Function Following SARS-CoV-2 Infection and A Long COVID Definition: A Chronic, Systemic Disease State with Profound Consequences.

Longitudinal changes in regional fat and muscle composition and cardiometabolic biomarkers over 5 years of hormone therapy in transgender individuals.

Longitudinal studies investigating hormone therapy in transgender individuals are rare and often limited to 1- to 2-year follow-up periods. We examined changes in body composition, muscle volumes, and fat distribution as well as muscle strength, arterial stiffness, and cardiometabolic biomarkers in both transgender men (TM; n=17, age 25±5 years) and transgender women (TW; n=16, age 28±5 years) at baseline and after 1 and 5-6 years of hormone therapy in a longitudinal prospective cohort design. Whole-body and regional fat and muscle volumes were analyzed using magnetic resonance imaging, and blood samples were taken. Skeletal muscle size increased in TM (21% after 6 years) and decreased in TW (7% after 5 years). Muscle strength increased 18% after 6 years in TM (p=0.003) but was statistically unchanged in TW. Muscle fat infiltration changed (p<0.05) almost completely toward the affirmed sex phenotype after 1 year of therapy in both TM and TW. The most notable changes in fat volume distribution were that TW increased total adiposity but decreased visceral fat volume, whereas TM showed increased visceral fat (70%) and liver fat but relatively stable total adipose tissue levels. Although arterial stiffness and blood pressure did not change, there was a significant increase in triglyceride and LDL cholesterol levels and a decrease in HDL levels in TM after 6 years. These unique longitudinal data underscore the importance of continued clinical monitoring of the long-term health effects of gender-affirming hormone therapy in both TW and, perhaps especially, TM.

Changes in the intestinal microbiota of Japanese children during the first 3.5 years of life

Human gut microbiota plays a crucial role in health and disease. Infancy is a critical period for gut microbiota maturation and immune system development and has the potential to affect long-term health. Understanding the development of gut microbiota in Japanese children is essential because of regional differences and the long-term health effects of the early gut microbiota. However, while several longitudinal studies in Japan have explored the development of the gut microbiota after birth, more extended follow-up periods are still needed. In this study, we aimed to analyze the gut microbiota of 106 Japanese mother–child pairs from the Chiba Study of Mother and Child Health, Japan, over 3.5 years. The results showed that the alpha diversity of the gut microbiota in children increased with age, and its composition began to resemble that of adults. We identified four distinct clusters of gut microbiota that reflected different maturation stages. The similarity between the maternal and child gut microbiota appeared to follow a bimodal-like distribution, suggesting that the presence of older siblings may enhance this similarity. This study highlights the dynamic nature of gut microbiota development in Japanese children and deepens our understanding of the similarities between maternal and child gut microbiota.

The Effect of Maternal Diet and Lifestyle on the Risk of Childhood Obesity

Background/Objectives: Childhood obesity is a global health problem that affects at least 41 million children under the age of five. Increased BMI in children is associated with serious long-term health consequences, such as type 2 diabetes, cardiovascular disease, and psychological problems, including depression and low self-esteem. Although the etiology of obesity is complex, research suggests that the diet and lifestyle of pregnant women play a key role in shaping metabolic and epigenetic changes that can increase the risk of obesity in their children. Excessive gestational weight gain, unhealthy dietary patterns (including the Western diet), and pregnancy complications (such as gestational diabetes) are some of the modifiable factors that contribute to childhood obesity. The purpose of this narrative review is to summarize the most important and recent information on the impact of the diet and lifestyle of pregnant women on the risk of childhood obesity. Methods: This article is a narrative review that aims to summarize the available literature on the impact of pregnant women’s diet and lifestyle on the risk of obesity in their offspring, with a focus on metabolic and epigenetic mechanisms. Results/Conclusions: Current evidence suggests that a pregnant woman’s lifestyle and diet can significantly contribute to lowering the risk of obesity in their offspring. However, further high-quality research is needed to understand better the metabolic and epigenetic relationships concerning maternal factors that predispose offspring to obesity.

Long-Term Oral Health Effects of Traumatic Events Among World Trade Center Health Registry Enrolees, 2003-2020.

Posttraumatic stress disorder (PTSD) is a disabling mental health condition arising from experiencing serious traumatic events. This study investigated if PTSD secondary to the World Trade Center terrorist attack on 11 September 2001 (9/11) is associated with self-reported doctor's diagnosis of periodontitis and count of missing teeth. Data from the World Trade Center Health Registry, 2003-2020 (n = 20 826) were used to prospectively examine the rate of periodontitis and cross-sectionally the odds of missing teeth among enrolees with/without PTSD post-9/11 using a modified Poisson regression and generalised logit model, respectively, adjusted for covariates. Approximately 17% reported a doctor's diagnosis of PTSD post-9/11 and 7.4% a doctor's diagnosis of periodontitis. There were 355 new cases of periodontitis (incidence rate = 7.6 per 1000 person-years) among those with PTSD and a rate of 4.3 per 1000 person-years among those without PTSD. The covariate-adjusted rate ratio for periodontitis comparing those with and without PTSD = 1.52 (95% CI: 1.33, 1.74). The adjusted odds ratio (OR) (95% CI) comparing those with and without PTSD were, respectively, OR = 1.15 (95% CI: 1.05, 1.25) and OR = 1.41 (95% CI: 1.09, 1.83) for missing 1-5 teeth and all teeth missing, respectively, as compared to no missing teeth. PTSD post-9/11 appears to be associated with poorer oral health outcomes, underscoring the far-reaching consequences of traumatic events on oral health.

In vitro Cultured Meat: Nutritional Aspects for the Health and Safety of Future Foods

In vitro cultured meat is one of the future foods that might revolutionize meat production by allowing food manufacturers to sidestep traditional animal farming. Despite being in early development, in vitro cultured meat faces challenges such as technological limitations and consumer acceptance, hindering its market integration. Beyond being an ethical alternative to conventional meat, it resembles conventionally farmed meat, making it a focus on healthcare and food safety. From a health perspective, it theoretically presents intriguing possibilities in the fields of functional foods, tailored dietary therapy, and public health. In functional foods, its unique composition allows precise nutritional manipulation for specific dietary needs, aligning with personalized nutrition. In dietary therapy, in vitro cultured meat might offer customization for individuals with dietary restrictions or health conditions, providing a novel therapeutic avenue. From a public health standpoint, it holds promise in mitigating environmental and health challenges linked to conventional meat production, aligning with sustainability and disease prevention initiatives. However, the potential health risks of in vitro cultured meat include concerns about the use of genetically modified starting cells, fetal bovine serum, growth factors, scaffolding materials, and antibiotics during the production process. These additives may have unforeseen long-term health effects if not properly regulated. Additionally, in vitro cultured meat may lack some of the natural nutrients found in conventional meat, which could lead to nutritional imbalances. There are also uncertainties about how the texture and bioavailability of nutrients in in vitro cultured meat might affect digestion and absorption in the human body. Nevertheless, specific aspects of research in the field of in vitro cultured meat demand increased focus from researchers to guarantee the optimal safety standards. An in-depth study on safety-oriented research of in vitro cultured meat should be emphasized to ensure benefits for individual and public health. HIGHLIGHTS In theory, in vitro cultured meat offers potential as a novel solution for developing functional foods by allowing precise manipulation of nutritional content, enabling the creation of health-optimized products. In vitro cultured meat could help mitigate the risk of antimicrobial resistance by minimizing the need for antimicrobial agents, reducing overall exposure and the potential for the development and spread of antimicrobial-resistant microorganisms. Starting cells derived from genetically modified cells in the in vitro cultured meat may pose risks of allergenicity and contamination, as well as potential immune responses to novel proteins. Strict safety protocols and thorough testing are crucial to address these risks and ensure the final product is safe for consumption. GRAPHICAL ABSTRACT

PERLINDUNGAN HUKUM TERHADAP PEREMPUAN DIBAWAH UMUR AKIBAT PERKAWINAN DINI PERSPEKTIF MAQASHID SYARIAH

This study aims to evaluate the legal protection for underage girls vulnerable to early marriage in Indonesia, analyzed from the perspective of maqashid syariah. Early marriage has shown adverse impacts on health, education, and psychological well-being, raising concerns about the sufficiency of existing legal protections. This normative study employs a conceptual approach by examining Indonesian regulations, including Law No. 16 of 2019, which amends Law No. 1 of 1974 on Marriage, assessing its alignment with maqashid syariah principles that emphasize the protection of religion (hifz al-din), life (hifz al-nafs), intellect (hifz al-aql), progeny (hifz al-nasl), and wealth (hifz al-mal). Although the legal age for marriage is set at 19, the provision for marriage dispensations with court approval presents a gap that facilitates child marriages. The findings reveal that dispensations are frequently granted without considering the long-term physical and mental health consequences for young girls. This study underscores the need for stricter regulation on marriage dispensations and advocates for community education programs involving religious and educational leaders to raise awareness about the negative effects of early marriage. The recommendations align with broader efforts to protect girls through enhanced community engagement, rigorous oversight, and legal enforcement based on maqashid syariah principles, to effectively prevent child marriages in Indonesia.

Cord plasma metabolomic signatures of prenatal per- and polyfluoroalkyl substance (PFAS) exposures in the Boston Birth Cohort

BackgroundPrenatal per- and polyfluoroalkyl substance (PFAS) exposures are associated with adverse offspring health outcomes, yet the underlying pathological mechanisms are unclear. Cord blood metabolomics can identify potentially important pathways associated with prenatal PFAS exposures, providing mechanistic insights that may help explain PFAS' long-term health effects. MethodsThe study included 590 mother-infant dyads from the Boston Birth Cohort. We measured PFAS in maternal plasma samples collected 24–72 h after delivery and metabolites in cord plasma samples. We used metabolome-wide association studies and pathway enrichment analyses to identify metabolites and pathways associated with individual PFAS, and quantile-based g-computation models to examine associations of metabolites with the PFAS mixture. We used False Discovery Rate to account for multiple comparisons. ResultsWe found that 331 metabolites and 18 pathways were associated with ≥ 1 PFAS, and 38 metabolites were associated with the PFAS mixture, predominantly amino acids and lipids. Amino acids such as alanine and lysine and their pathways, crucial to energy generation, biosynthesis, and bone health, were associated with PFAS and may explain PFAS’ effects on fetal growth restriction. Carnitines and carnitine shuttle pathway, associated with 7 PFAS and the PFAS mixture, are involved in mitochondrial fatty acid β-oxidation, which may predispose higher risks of fetal and child growth restriction and cardiovascular diseases. Lipids, such as glycerophospholipids and their related pathway, can contribute to insulin resistance and diabetes by modulating transporters on cell membranes, participating in β-cell signaling pathways, and inducing oxidative damage. Neurotransmission-related metabolites and pathways associated with PFAS, including cofactors, precursors, and neurotransmitters, may explain the PFAS’ effects on child neurodevelopment. We observed stronger associations between prenatal PFAS exposures and metabolites in males. ConclusionsThis prospective birth cohort study contributes to the limited literature on potential metabolomic perturbations for prenatal PFAS exposures. Future studies are needed to replicate our findings and link prenatal PFAS associated metabolomic perturbations to long-term child health outcomes.

Maternal Perceptions and Feeding Practices Associated with Infant Nutritional Status.

Malnutrition in early childhood has long-term health consequences, including increased obesity risk due to catch-up growth in undernourished infants. While maternal perceptions and feeding practices affect infant malnutrition, limited evidence exists in middle-income countries like Mexico. This study examines the associations between maternal perceptions, feeding practices, and nutritional status in infants aged 6-12 months in northeastern Mexico. A total of 192 mother-infant dyads were assed using questionnaires and anthropometric measurements. Maternal variables included perception of hunger and satiety, infant weight perception, feeding attitudes, self-efficacy, fatigue, and feeding practices. Infant nutritional status was determined using WHO Anthro, and multiple logistic regression identified associations between maternal and infant factors and nutritional status. 38% of infants were overweight/obese, and 7% were underweight. Low maternal self-efficacy, low perception of hunger and satiety signs, non-recommended feeding attitudes, and inaccurate perception of infant weight were identified. Maternal nocturnal sleep hours, type of feeding, and feeding frequency were associated to underweight. The maternal perception of infant weight was inaccurate for both underweight (mothers overestimate the weight) and overweight/obese (mothers underestimate the weight) infants. The main maternal perception influencing infant nutritional status was inaccurate weight perception. Mothers demonstrated limited knowledge and negative feeding practices with non-recommended feeding practices among Mexican mothers. Longer maternal sleep duration (> 8h/night) was associated with underweight infants.

Altered mammary gland development and pro-tumorigenic changes in young female mice following prenatal BPAF exposure

Bisphenol A (BPA) is being phased out owing to its endocrine-disrupting effects and is increasingly being replaced by its substitute compounds such as bisphenol AF (BPAF). This study aims to explore the potential adverse outcomes of prenatal BPAF exposure combined with postnatal cross-fostering on the development and long-term health effects of the mammary gland in offspring. The results suggested that prenatal BPAF exposure accelerates the puberty, and induces duct dilatations, angiogenesis, lobular hyperplasia, and enhanced inflammatory cell infiltration in the mammary gland of female offspring. Differentially expressed genes exhibiting time series patterns induced by BPAF exposure were enriched in biological processes related to mammary gland development, epithelial cell proliferation and so on. Notably, 13 breast cancer-related biomarkers including Pgr, Gata3, Egfr and Areg were screened, showing a time-dependent increase in expression. After human homologous gene transformation, TCGA analysis suggested that the human homologues of genes differentially expressed in BPAF-treated mice were associated with increased tumor stages in female patients with breast cancer. Furthermore, postnatal cross-fostering did not completely restore the adverse effects of prenatal BPAF exposure and even showed a reverse tendency. These results imply that prenatal BPAF exposure in utero and postnatally nursing by BPAF exposed dams, have long-term effects on the mammary glands health of female offspring.

Sex and tissue-specificity of piRNA regulation in adult mice following perinatal lead (Pb) exposure

ABSTRACT Lead (Pb) is a neurotoxicant with early life exposure linked to long-term health effects. Piwi-interacting RNAs (piRNAs) are small non-coding RNAs that associate with PIWIL proteins to induce DNA methylation. It remains unknown whether Pb exposure influences piRNA expression. This study evaluated how perinatal Pb exposure (32 ppm in drinking water) impacts piRNA expression in adult mice and assessed piRNA dysregulation as a potential mechanism for Pb-induced toxicity. Pb exposure effects on piRNA expression and associated gene repression in the germline (testis/ovary) and soma (liver and brain) were evaluated. Small RNA sequencing was used to determine differentially expressed piRNAs, RT-qPCR to examine piRNA target expression, and whole genome bisulfite sequencing to evaluate target DNA methylation status. Three piRNAs (mmpiR-1500602, mmpiR-0201406, and mmpiR-0200026) were significant after multiple testing correction (all downregulated in the male Pb-exposed brain in comparison to control; FDR < 0.05). Within piOxiDB, TAO Kinase 3 was identified as a downstream mRNA target for one of the three Pb-sensitive piRNA. The Pb-exposed male brain exhibited increased Taok3 expression (p < 0.05) and decreased DNA methylation (FDR < 0.01). The results demonstrate that perinatal Pb exposure stably influences longitudinal piRNA expression in a tissue- and sex-specific manner, potentially via DNA methylation-directed mechanisms.