Long-term Studies Research Articles

Divergent temporal responses of native macroinvertebrate communities to biological invasions.

Biological invasions pose a major threat to biodiversity, ecosystem functioning, and human well-being. Non-native species can have severe ecological impacts that are transformative, affecting ecosystems across both short-term and long-term timescales. However, few studies have determined the temporal dynamics of impact between these scales, impeding future predictions as invasion rates continue to rise. Our study uses a meta-analytical approach to dissect the changing taxonomic and functional impacts of biological invasions on native macroinvertebrate populations and communities in freshwater ecosystems across Europe, using a recently collated European long-term time series spanning several decades. Our findings reveal a complex temporal pattern: while initial stages of invasions (i.e. five years after the first record of non-native species) often exhibited benign impacts on macroinvertebrate abundance, richness, or functional diversity, the long-term (i.e. the period following the early invasion) effects became predominantly negative. This pattern was consistent between taxonomic and functional metrics for impacts at both the population and species level, with taxonomic metrics initially positively affected by invasions and functional metrics being more stable before also declining. These results suggest that even initially benign or positively perceived impacts could be eventually superseded by negative consequences. Therefore, understanding the magnitude of invasion effects increasingly requires long-term studies spanning several years or decades to offer insights into effective conservation strategies prioritising immediate and future biodiversity protection efforts. These findings also highlight the importance of integrating multiple taxonomic, functional and temporal components to inform adaptive management approaches to mitigate the negative effects of current and future biological invasions.

Network technologies as a tool for self-presentation of small religious groups (the case of Buddhism new for Russia)

This article analyses the results of a long-term study of the strategies of the Russian-speaking Buddhist community in relation to the Internet and new media. The authors present the results of a study of two cases of Buddhist digital innovators - communities of the Russian-speaking branch of the International Dzogchen Community and Russian Theravada. The authors chose these two cases for analysis, since they are representative for the sociological consideration of the multidimensional connections between online and offline communications of Russian digital Buddhism. The subject of the study seems to be especially relevant, since in modern sociology more and more attention is paid to the study of the influence of media technologies, the Internet and social media on religious traditions, practices and forms of social organisation of religion. The reason for this is the deepening process of mediatisation of modern societies. It has brought with it fundamental transformations of various spheres of social activity. Religions are involved in the process of mediatisation, that poses a number of serious questions that require new answers unknown to their traditions. Thus, the digitalisation of sacred texts brings with it the devaluation of the tradition of canonical knowledge and interpretation of sacred texts. In this complex context of reformatting social reality, Russian traditional religions are developing their own reflections and responses to the risks of mediatisation. On the Russian-language Internet, Orthodoxy, Islam, Judaism and Buddhism are represented by a rich variety of websites of the corresponding religious communities and centres of various trends, podcasts, channels, publics, groups and blogs on social networks. And, as the results of sociological research demonstrate, the discussion of the correlation of the value-normative coordinates of everyday life with religious-doctrinal regulation serves as the semantic core of trans-local religious communications mediated by media. New digital media challenge religious traditional values, since they provide space and ways of forming new religious authorities, new ways of influencing a certain religious group. The study was conducted using a methodology developed by the authors, combining methods of mathematical modelling and qualitative sociology. The authors conclude that, paradoxically, non-traditional Buddhist organisations in Russia have adopted an innovative strategy and have proven to be very creative in attracting new media and promoting their own presentations in the public space. And the directions of Buddhism that have been institutionalised in the past 30 years, on the contrary, are conservative.

Sphingosine-1-phosphate receptor type 4 is critically involved in the regulation of peritoneal B-1 cell trafficking and distribution in vivo.

B-1 cells are crucially involved in immune defense and regulation of inflammation and autoimmunity. B-1 cells are predominantly located in the peritoneal and pleural cavities, although body cavity B-1 cells recirculate systemically under steady-state conditions. The chemokines CXCL12 and CXCL13 have been identified as the main regulators of peritoneal B-cell trafficking. In mice deficient for sphingosine-1-phosphate receptor 4 (S1PR4), B-1a and B-1b cell numbers are reduced in the peritoneal cavity by an unknown mechanism. In this study, we show that S1PR4-mediated S1P signaling modifies the chemotactic response of peritoneal B cells to CXCL13 and CXCL12 in vitro. In vivo, S1PR4-mediated S1P signaling affects both immigration into and emigration from the peritoneal cavity. Long-term reconstitution experiments of scid mice with wt or s1pr4 -/- peritoneal B cells revealed a distinct distributional pattern in secondary lymphoid organs. As a functional consequence, both plasmatic and mucosal IgM levels, the main product of B-1a cells, are reduced in mice reconstituted with s1pr4 -/- peritoneal cells. In summary, our data identify S1PR4 as the second S1P receptor (besides S1PR1), which is critically involved in the regulation of peritoneal B-1 cell function.

Longevity records for bird species from the central Monte desert, Argentina

ABSTRACT Longevity is a key life-history parameter of bird species, highly relevant for the development of evolutionary biology, the study of ecological patterns and conservation planning. However, there is a marked paucity of data for Neotropical birds, mainly because estimating longevity requires long-term studies that are very scarce in temperate South America. We report data on the longevity of 25 bird species obtained in an 8-year long-term capture–recapture programme carried out in the Monte desert in Argentina and evaluate the influence of diet and migratory status on this life-history trait. The maximum value recorded was 105 months for Microspingus torquatus and the minimum was 13 months for Synallaxis albescens. Resident birds had higher average longevity than migratory birds, while species consuming mainly plant material had higher longevity values than those consuming mainly animal foods. Our results are similar to the few published records from temperate South America, although more data are needed to assess the impact of diet and migratory behaviour on longevity of Neotropical species.

Contribution of distinct components in divergent organic amendments to long-term accumulation of soil organic matter in agricultural soils: evidence from thermally assisted hydrolysis and methylation-GC/MS.

Although maintaining/improving soil organic matter (SOM) quantity through utilizing organic amendments (OAs) is a productive practice, information on OA components contributing to long-term SOM accumulation in agricultural soils remains meager. Hence, we examined soil samples from a long-term experiment with different fertilizer managements, including no fertilizer (NF), chemical fertilizer (CF) only (CF), bark compost plus CF (BC + CF), coffee residue compost plus CF (CRC + CF), cattle manure compost plus CF (CMC + CF), and cattle manure compost (CMC) or sewage sludge compost (SSC) alone at a higher application rate. SOM in those samples was physically fractionated into free particulate form (fSOM), free form occluded in aggregates (oSOM), weakly bound form (wSOM), and strongly bound form (sSOM). Analysis of structural components in OAs and SOM fractions using thermally assisted hydrolysis and methylation combined with gas chromatography/mass spectrometry (THM-GC/MS) revealed OA components that have contributed to long-term SOM accumulation in each treatment. The SSC was characterized by higher proportions of short-chain fatty acids (FAs; 50%) and other aliphatic compounds (22%). Correlation analysis suggested that these characteristics may be effective for greater C accumulation in the bulk soil and wSOM fraction. The proportion of lignin-derived phenols, e.g., 3,4-dimethoxybenzaldehyde (Vh) and 1,2-dimethoxy-4-(1,2,3-trimethoxypropyl)benzene, was high in CMC (43%), presumably vital for promoting C accumulations in the oSOM, wSOM, and sSOM fractions in the CMC treatment. The short-chain FAs in CRC and aromatic components in BC are likely important for contributing fSOM accumulation.

Integrated crop management for long-term sustainability of maize-wheat rotation focusing on productivity, energy and carbon footprints

Modern agriculture, driven by diesel-fed machinery, is both energy and carbon-intensive, primarily due to the improper use of inputs. Designing a production module with lower carbon emissions, greater productivity, and enhanced energy use efficiency is crucial for achieving the environmental sustainability. Conservation agriculture based integrated crop management (ICMs) practices have shown promise in improving the productivity, energy-use, and reducing the carbon emissions. In our extensive long-term field study, we explored the crop yields, energy use, and carbon linkages of a maize-wheat rotation under different ICMs practices. Eight ICMs were evaluated over the course of nine consecutive years, which included ICM1&2: conventional (CT) flat-bed maize and wheat, ICM3&4: CT raised bed maize fb wheat without residues, ICM5&6: conservation agriculture (CA)-based double zero tilled (ZT) maize fb ZT wheat with residues and ICM7&8: CA-based triple ZT maize and wheat with residues and green manures. Our results indicated that the system productivity under CA-based ICMs was 16.5–22.9% greater than that under the CT-based ICM1-4. CA based ICM5-8 (125.2–131.5 × 103 MJ ha−1) needed higher energy input than CT-based ICM1-4 (32.7–39 × 103 MJ ha−1), with 76–80% shared by renewable crop residues. Furthermore, the CA-based ICMs recorded significantly higher levels of specific energy, human energy profitability, non-renewable energy use efficiency, and nutrient energy use efficiencies compared to CT-based. Likewise, the CA-based ICMs produced 12.5% more total output energy than the CT-based ICMs. In contrast, the CT based ICMs demonstrated, energy productivity, higher net energy returns and energy profitability. Compared to conventional ICMs, CA-based ICMs produced a 23–36% reduction in spatial carbon emissions and a 31–44 % reduction in yield-scale emissions. Nevertheless, the CA-based practices recorded the higher C- output, C- sustainability and C- efficiency ratio. Greenhouse gas intensity (GHGI) was 33.3–76.2% greater with the ICM1-4 practices than with the ICM5-8 practises. Thus, the present study proposed that adopting the integrated crop management practices based on the conservation agriculture could prove to be productive, as well as carbon and energy-use efficient approach for the maize-wheat rotation. This approach is envisioned to make substantial contributions to both food security and environmental sustainability.

Unveiling correlations between aristolochic acids and liver cancer: spatiotemporal heterogeneity phenomenon.

Aristolochic acids are a class of naturally occurring compounds in Aristolochiaceae that have similar structural skeletons and chemical properties. Exposure to aristolochic acids is a risk factor for severe kidney disease and urinary system cancer. However, the carcinogenicity of aristolochic acids to the liver, which is the main site of aristolochic acid metabolism, is unclear. Although the characteristic fingerprint of aristolochic acid-induced mutations has been detected in the liver and aristolochic acids are known to be hepatotoxic, whether aristolochic acids can directly cause liver cancer is yet to be verified. This review summarizes the findings of long-term carcinogenicity studies of aristolochic acids in experimental animals. We propose that spatiotemporal heterogeneity in the carcinogenicity of these phytochemicals could explain why direct evidence of aristolochic acids causing liver cancer has never been found in adult individuals. We also summarized the reported approaches to mitigate aristolochic acid-induced hepatotoxicity to better address the associated global safety issue and provide directions and recommendations for future investigation.

Students’ participation in transnational higher education: motivations for choosing a short-term study abroad programme

ABSTRACT Short-term study abroad programmes are increasingly recognised as pivotal components of transnational higher education and increasing in prominence across university landscapes. However, the scholarly discourse predominantly focuses on long-term study abroad experiences, and needs more research concerning short-term engagements. This study seeks to address this by exploring students’ motivations – a critical element of transnational educational exchanges – for participating in a short-term Sino-Canadian study abroad programme. Employing expectancy-value theory, the research utilises a mixed-methods approach, including interviews, observations, and summative reports, conducted before, during, and after the programme to assess the participants’ expectations and perceived values. The findings reveal a compensatory relationship between expectancy and value, as well as between value and cost, which significantly influences student participation decisions. This study underscores the implications of these dynamics within the broader context of transnational higher education, aiming to enhance programme design and improve participant experiences in short-term study abroad settings.

Embodied suffering: Uncovering the illness experiences of patients with severe psoriasis.

The visibility of skin lesions significantly burdens people with psoriasis, leading to social hostility and numerous emotional and psychological problems. These issues adversely affect self-esteem, can result in chronic mental health challenges and cause numerous life problems. This study aimed to explore patients' long-term experiences with severe psoriasis. A qualitative study was conducted with 20 patients with psoriasis (PASI ≥12) recruited from general and specialist dermatology practices in a regional teaching hospital in Taiwan. Interviews lasted 60-90 min and data were analysed using content analysis. A core theme emerged: 'Embodied suffering-life worse than death'. This overarching concept comprised three interrelated themes: (i) Experiencing physical suffering, (ii) Experiencing psychological suffering and (iii) Experiencing the stigma of suffering. This study highlights the holistic nature of suffering among individuals with severe psoriasis. It emphasises the need for healthcare professionals to consider the entirety of a patient's circumstances when addressing their suffering.

The effect of allergen immunotherapy in patients with central compartment atopic disease post-surgery.

To assess the effect of allergen immunotherapy (AIT) on patients with central compartment atopic disease (CCAD) and house dust mite (HDM) sensitization post-surgery. A retrospective cohort of surgically treated, HDM-sensitized CRSwNP patients phenotyped as CCAD was assessed. Patients were divided into two groups based on whether they had AIT commenced as part of their surgical care. All AIT patients started immunotherapy prior to their surgery. The primary endpoint was reformation of middle turbinate (MT) edema 12 months postsurgery. Secondary endpoints were corticosteroid irrigation use (<4 times/week vs. ≥4 times/week, %) and the rhinologic domain of the 22-item sino-nasal outcome test (SNOT-22). Demographic characteristics, concomitant asthma, smoking status, history of aspirin-exacerbated respiratory disease, conjunctival symptoms, polysensitization, serum eosinophils (cell×109/L), tissue eosinophilia (%>100/HPF), and serum IgE (kU/L) were also recorded. Eighty-six CCAD patients were assessed (41±14yrs, 64% female). AIT was applied in 37% (n=32). Baseline features were similar apart from greater conjunctival symptoms (72 vs. 45%, p=0.02) in the AIT group. At 12 months post-surgery, the AIT group has less MT edema (%≥diffuse 15.6 vs. 52.9, p<0.01). Patients on AIT also had less pharmacotherapy requirements at 12 months (%≥4/week, 37.5 vs. 79.6%, p<0.01). The rhinologic symptoms were similar (21.1±17.1 vs. 20.1±21.6, p=0.83). Surgery and pharmacotherapy are effective in managing CCAD, but the addition of AIT improved allergic phenomenon and allowed de-escalation of topical therapy. Longer term studies are required to demonstrate further immunomodulation.

Modelling and validating soil carbon dynamics at the long-term plot scale using the rCTOOL R package

We introduce rCTOOL, an open-source R package for carbon (C) turnover modelling, featuring comprehensive documentation and a user-friendly interface. As an enhanced version of the widely used Danish C-TOOL model, rCTOOL maintains minimal input data requirements and reliable performance, while addressing the original model's limitations in openness and documentation. To validate rCTOOL, we analysed topsoil Soil Organic Carbon (SOC) dynamics using data from the long-term Askov straw disposal experiment (1981–2019), which quantifies the impact of varying annual straw C inputs on SOC storage. Our validation shows an unbiased global prediction error of less than 10%, with a mean error of 1.1 Mg C/ha (CI -0.7–3.0 Mg C/ha). The discrepancies between observed and predicted values were primarily due to spatiotemporal variability represented by the block and year in the long-term field experiment. We demonstrate how rCTOOL is a reliable asset for diverse applications in SOC management and research.

Solar transpiration-powered lithium extraction and storage.

Lithium mining is energy intensive and environmentally costly. This is because lithium ions are typically present in brines as a minor component mixed with physiochemically similar cations that are difficult to separate. Inspired by nature's ability to selectively extract species in transpiration, we report a solar transpiration-powered lithium extraction and storage (STLES) device that can extract and store lithium from brines using natural sunlight. Specifically, the device uses a hierarchically structured solar transpirational evaporator to create a pressure gradient, which allows for the extraction of lithium from brines through a membrane and its storage in a vascular storage layer. Long-term experiments, various membrane tests, and different size assessments demonstrate the stability, compatibility, and scalability of STLES. This solar-powered mining technology provides an alternative developing pathway toward the sustainable extraction of critical resources.

Incorporation of undissolved lime from previous applications can ameliorate subsoil acidity promptly and improve crop performance on sandy soils of the semi-arid regions of Western Australia

Abstract Background and aims Repeated surface application of lime for managing subsoil acidity is slow and ineffective, resulting in an accumulation of undissolved lime (carbonate) in the topsoil. We investigated the impact of the incorporation of undissolved lime into the subsoil to improve acidity and crop performance. Methods The undissolved lime in 2-cm layers of topsoil (0–10 cm) from three long-term experiments in Western Australia was measured. Both limed and unlimed topsoil with the acidic subsoil of the same profile was incubated at eight incorporation rates for six weeks, followed by growing barley and wheat in the incubated soil for two weeks to assess the impact on soil acidity and crop root architecture, respectively. Furthermore, a three-year-long field experiment was conducted following strategic tillage in limed and control plots to assess the impact on soil acidity and performance of wheat, canola and barley. Results A significant amount of undissolved lime was concentrated in the topsoil, amounting to 1.7, 1.8 and 1.3 t/ha for the limed plots at Wongan Hills, Northam and Merredin, respectively. Incubation of 5–25% topsoil after incorporation with the acidic subsoil was enough to ameliorate subsoil acidity and to improve root length density by up to 13-fold depending on undissolved lime content in topsoils and soil type. In the field experiment, the incorporation of undissolved lime also significantly improved subsoil acidity and canola performance. Conclusion We concluded that the incorporation of topsoil containing sufficient undissolved lime with acidic subsoil may offer a quick amelioration of subsoil acidity.

Communication Skills in Toddlers Exposed to Maternal SARS-CoV-2 during Pregnancy.

Studies about the effects of SARS-CoV-2 on pregnant women and children born to positive women are controversial with regard to possible inner ear-related damage but most of them do not detect the involvement of this virus in auditory function. However, only a few studies on long-term effects on language development are currently available because of the recent onset of the pandemic. The aim of this study was to investigate the impact of SARS-CoV-2 infection on perceptual and expressive abilities and the emerging development of communication in young children. To this purpose, the MacArthur-Bates Communicative Development Inventory-Words and Gestures form (CDI-WG), was administered to parents. In total, 115 children whose mother was infected by SARS-CoV-2 during pregnancy were enrolled in the study and evaluated at the Audiology Service of the Modena University Hospital. All children underwent Otoacoustic Emissions (OAE) at birth: 114/115 had a "pass" result bilaterally, while 1 case had a unilateral "refer" result. Overall, 110/115 newborns (95.65%) underwent audiological evaluation between 10-18 months of age. In 5/110 patients (3.6%), the Pure Tone Average (PTA) result was equal to 35 dB; one case had a hearing threshold of around 50 dB due to a bilateral effusive otitis media. A notable finding was the percentage of children with tubal dysfunction in both evaluations, within 2 months of age and around 12 months of age. Most children revealed normal hearing. The CDI-WG was completed by 56/115 families. The rate of children below the fifth percentile was 8.9% for sentences understood, 12.5% for words understood, and 5.4% for words produced. Concerning CDI-Gestures, only 2 children (3.6%) were below the fifth percentile. A structured audiological follow-up in association with the evaluation of communication skills of children appears fundamental, particularly in the years of maximum neuroplasticity. Long-term studies are still necessary to evaluate the possible consequences of the pandemic.

A systematic review on the techniques, long-term outcomes, and complications of partial breast irradiation after breast-conserving surgery for early-stage breast cancer.

To evaluate the efficacy and safety of four techniques of partial breast irradiation (PBI) including interstitial brachytherapy (ISBT), balloon-based brachytherapy (BBT), Intraoperative radiotherapy (IORT) and three-dimensional conformal radiotherapy (3DCRT) in the treatment for early-stage breast cancer patients after breast-conserving surgery. A systematic search was performed according to the Preferred Reporting Items of Systematic Reviews and Meta-analyses (PRISMA) guidelines using the PubMed, Embase, Cochrane Library and Web of Science databases. The inclusion criteria were clinical trials and observational studies that reported on outcome measures of principal PBI techniques. The methodological quality of the included research data was assessed using bias risk assessment tool with the Methodological Index for Non-Randomized Studies (MINORS), and the research information were analyzed using data analysis software. Clinical studies were collected from the earliest available date until September 2023. Fifty-one studies were included, with a total sample size of 7708. The results of network meta-analysis (NMA) showed that ISBT can lower the local recurrence (SUCRA: 73.8%). In terms of reducing distant metastasis, 3DCRT may be the best choice (SUCRA: 52.5%). And IORT has the highest 5-year overall survival (SUCRA: 90%). Furthermore, ISBT also has the advantages of lowest risk with fat necrosis (SUCRA: 72.5%), infection (SUCRA: 78.3%) and breast pain (SUCRA: 86.2%). BBT may be the optimal solution for fibrosis (SUCRA: 76.9%) and hyperpigmentation (SUCRA: 66.7%). 3DCRT has lower incidence of telangiectasia (SUCRA: 56.7%) and better cosmetic result (SUCRA: 85%). Postoperative PBI treatment using ISBT after breast-conserving surgery in patients with early-stage breast cancer may be a more valuable choice based on the treatment efficacy and is associated with fewer late side-effects. Large-scale, prospective, long-term studies are warranted to clarify the role of different PBI techniques in selected patients.

The Role of Stress in Obesity and Relevant Interventions

The prevalence of obesity is on the rise, which significantly increases the burden on global healthcare expenditures and profoundly impacting both physical and mental health. It has become a major public health challenge that urgently needs to be addressed in todays society. Obesity is influenced by multiple factors, including stress, genetics, hormones, and neuroendocrine elements, with lifestyle and epigenetic changes also playing significant roles. Stress impacts various physiological systems, including the brain, memory, cognition, immune response, cardiovascular health, and endocrine function. It can also trigger obesity by affecting appetite regulation, reward sensitivity, and hormonal balance. Understanding the relationship between stress and obesity is crucial for developing effective interventions. Recent research highlights that stress significantly affects eating behaviors, leading to weight gain, while obesity can exacerbate stress and disrupt life balance. Effective interventions include mindfulness training, which improves self-awareness, positive emotions, and psychological flexibility, and reduces stress, anxiety, and depression. Stress management programs and physical activity are also beneficial for weight loss and overall health. However, the limitations of small sample sizes, short intervention periods, limited participant diversity, and reliance on self-reported measures may undermine the generalizability and accuracy of the findings of previous research. Future research should focus on larger, diverse samples, long-term studies, and incorporate both objective measures of stress and self-reported data to better understand and manage obesity and stress.

Creating a bacterium that forms eukaryotic nucleosome core particles

The nucleosome is one of the hallmarks of eukaryotes, a dynamic platform that supports many critical functions in eukaryotic cells. Here, we engineer the in vivo assembly of the nucleosome core in the model bacterium Escherichia coli. We show that bacterial chromosome DNA and eukaryotic histones can assemble in vivo to form nucleosome complexes with many features resembling those found in eukaryotes. The formation of nucleosomes in E. coli was visualized with atomic force microscopy and using tripartite split green fluorescent protein. Under a condition that moderate histones expression was induced at 1 µM IPTG, the nucleosome-forming bacterium is viable and has sustained growth for at least 110 divisions in longer-term growth experiments. It exhibits stable nucleosome formation, a consistent transcriptome across passages, and reduced growth fitness under stress conditions. In particular, the nucleosome arrays in E. coli genic regions have profiles resembling those in eukaryotic cells. The observed compatibility between the eukaryotic nucleosome and the bacterial chromosome machinery may reflect a prerequisite for bacteria-archaea union, providing insight into eukaryogenesis and the origin of the nucleosome.

Climate adaptive loci revealed by seascape genomics correlate with phenotypic variation in heat tolerance of the coral Acropora millepora

One of the main challenges in coral reef conservation and restoration is the identification of coral populations resilient under global warming. Seascape genomics is a powerful tool to uncover genetic markers potentially involved in heat tolerance among large populations without prior information on phenotypes. Here, we aimed to provide first insights on the role of candidate heat associated loci identified using seascape genomics in driving the phenotypic response of Acropora millepora from New Caledonia to thermal stress. We subjected 7 colonies to a long-term ex-situ heat stress assay (4 °C above the maximum monthly mean) and investigated their physiological response along with their Symbiodiniaceae communities and genotypes. Despite sharing similar thermal histories and associated symbionts, these conspecific individuals differed greatly in their tolerance to heat stress. More importantly, the clustering of individuals based on their genotype at heat-associated loci matched the phenotypic variation in heat tolerance. Colonies that sustained on average lower mortality, higher Symbiodiniaceae/chlorophyll concentrations and photosynthetic efficiency under prolonged heat stress were also the closest based on their genotypes, although the low sample size prevented testing loci predictive accuracy. Together these preliminary results support the relevance of coupling seascape genomics and long-term heat stress experiments in the future, to evaluate the effect size of candidate heat associated loci and pave the way for genomic predictive models of corals heat tolerance.

Optimization of sampling design for soil total organic carbon assessment in the precision agriculture framework: Impact of different variogram models and potentiality of ground penetrating radar (GPR) covariate information

Assessing soil organic carbon (SOC) at the field scale is crucial for efficient environmental and agronomic management, especially within the precision agriculture framework. This enables the implementation of crop and soil management strategies to enhance soil quality, increase carbon sequestration, and improve crop yields. However, the process of sampling and assessing SOC is resource-intensive, demanding both in time and labour. This aspect is particularly relevant in agronomic research, where the need to assess the spatial correlation structure of the investigated variables requires the collection of large datasets with georeferenced data and often measurements need to be repeated over time as in the case long-term field experiments − LTE. Methods for minimizing information loss while reducing the sampling scheme, such as spatial simulated annealing (SSA), are particularly valuable in the scope of SOC assessment, especially when faced with budget and time/labour constraints. Within the structure of the SSA method, two critical components can be identified: i) the inclusion of highly informative covariates for the primary variable (SOC); ii) the selection of the most appropriate variogram model for spatial variability assessment. Covariates strongly correlated with SOC, such as those obtained from ground penetrating radar (GPR) that can be collected at a higher spatial density compared to SOC data, along with a well-performing model, can significantly enhance the efficiency of the sampling scheme reduction process. We conducted a study using data from an agronomic field experiment, which included 71 georeferenced sampling locations and, through an iterative downsizing process utilizing SSA, we progressively reduced the number of sampling points by removing 10, 15, and 20 observations. The sampling scheme was refined according to two distinct variogram models: the spherical and Gaussian-Matérn models, both for the primary variable (SOC) and the covariate GPR variable. This process allowed us to identify the optimal variogram model, which has a key role in maximizing the reduction of redundant points while preserving those with valuable information, and to assess the role of the covariate variable both in improving the optimization of the sampling scheme for SOC and in replacing the primary variable to optimize the sampling scheme. Finally, to assess the impact of sampling scheme reduction, a validation process was performed by estimating the dropped points by means of the remaining points using ordinary kriging and regression kriging and analysing the accuracy of such estimation. Our analysis demonstrated that it was possible to reduce the original sampling scheme by approximately 20%, equivalent to eliminating 15 sampling points out of 71, without compromising its predictive capability.

Deciphering Anion Exchange Membrane Water Electrolysis: A Distribution of Relaxation Times Approach

Anion exchange membrane water electrolysis (AEM-WE) is a promising method for hydrogen production, offering advantages over proton exchange membrane water electrolysis (PEM-WE), such as the use of nonprecious metal catalysts and perfluorosulfonic acid free membranes. Despite achieving impressive current densities, challenges in efficiency and stability remain. This study employs electrochemical impedance spectroscopy (EIS), the equivalent circuit model (ECM) and distribution of relaxation times (DRT) analysis to investigate AEM-WE cells. DRT analysis identifies and quantifies five loss mechanisms within the AEM-WE system, including hydrogen and oxygen evolution reactions and ionic transport losses. Long-term experiments reveal catalyst degradation and its impact on performance, providing insights for targeted optimisation. The findings enhance understanding of the electrochemical processes in AEM-WE, offering pathways to improve stability and efficiency.