Long-term Carbon Sequestration Research Articles

Carbon Sequestration of Common Garden Tree Species under the Carbon Neutrality Target in East China

The global warming phenomenon caused by greenhouse gas emission leads to the deterioration of the ecological environment. In urban spaces, the selection of garden tree species with high carbon sequestration rates can effectively contribute to carbon neutrality. In this study, we measured the height, diameter at breast height, and crown width of 643 ancient trees around the West Lake Scenic Spot, Hangzhou, China, and recorded their species and ages. By the biomass expansion factor method, the long-term carbon sequestration of the trees was calculated, and the corresponding statistical analysis indicated the following findings: (1) The maximum carbon sequestration of ancient trees varies with the species; the simple rational function has the best fit for the relationship between mean annual carbon sequestration and age. (2) For the five most common species in the Hangzhou area, the total individual amount of carbon sequestration per tree species can be ranked from high to low as follows: Celtis julianae, Cinnamomum camphora, Castanopsis sclerophylla, Liquidambar formosana, and Ginkgo biloba (tree age < 260 years). The ranking for trees aged above 260 years is as follows: Celtis julianae, Cinnamomum camphora, Liquidambar formosana, Castanopsis sclerophylla, and Ginkgo biloba. (3) The transient and mean annual carbon sequestration rate decreases as tree age increases; for most of the ancient trees in this research, the main growing period is 0–300 years. (4) Castanopsis sclerophylla, Liquidambar Formosana, and Osmanthus fragrans are recommended for urban landscape greening as they provide continuous long-term carbon sequestration and special landscape features.

Surface characterization of biochar from agricultural residues for environmental applications

Bio-waste materials banana (Musa ealbisiana) leaves, cassava (Manihot esculenta) peels, mango (Mangifera indica) leaves, plantain (Plantago lanceolata) leaves and peels were pyrolyzed at different temperatures; 300, 400, 450, 500 and 600 oC using a muffle furnace. The biochar samples obtained were characterized and properties evaluated for their potential application in soil for agronomic and environmental benefits. The measured physico-chemical properties; pH, bulk density, percentage yield and electrical conductivity varied with temperature of pyrolysis and somewhat with the source of the bio-waste. Surface morphology and elemental composition were determined from Scanning Electron Microscopic (SEM) and Energy Dispersive X-ray (EDX) measurements and showed variations in texture, porosity and elemental composition with pyrolysis temperature. The produced biochar yields were in the range 16.1 to 84.6% and highest for banana leaves (BL) pyrolysed at 300 oC. The scanned images showed a remarkable surface morphology for all the biochar samples obtained at temperatures ≥ 450 oC. The pH obtained indicate the alkaline nature of the biochars with mean values > 7.5 except for cassava peels (CP) pyrolysed at 300 oC having a value of 5.5. From the elemental analysis, the high carbon content (> 60%) at higher temperatures (> 450 oC) of the biochars and the molar O/C ratios may suggest the suitability of the biochars in applications for long-term carbon sequestration and also promising candidates for soil amendment due to increase in macro porosity.

Enhancing carbon reduction and sustainable agriculture in Thailand: An assessment of rice straw utilization strategies

This research explores strategies for carbon reduction through the utilization of rice straw waste in Thailand, focusing on three project approaches: (1) converting rice straw into biomass fuel pellets for power plants, (2) transforming rice straw into biochar using high-tech systems and blending it with fertilizers, and (3) using conventional systems for biochar production. The study assesses the greenhouse gas (GHG) reduction potential of these approaches compared to traditional open-field burning of rice straw. The results indicate that Project 2, which involves high-tech biochar production, exhibits the highest potential for GHG reduction and carbon sequestration, with a potential to reduce emissions by approximately 12.19–13.05 MTCO2e/year. This approach also has potential for generating valuable carbon credits due to biochar’s long-term carbon sequestration and soil enhancement benefits. In contrast, Project 1 yields a reduction of about 4.06–4.35 MTCO2e/year, while Project 3 results in negative emission reduction. The study recommends prioritizing and incentivizing biochar production projects, upgrading low-tech systems, balancing the use of biomass fuel pellets, leveraging carbon credits for funding, and enhancing public awareness.

Decoding drivers of carbon flux attenuation in the oceanic biological pump

The biological pump supplies carbon to the oceans’ interior, driving long-term carbon sequestration and providing energy for deep-sea ecosystems1,2. Its efficiency is set by transformations of newly formed particles in the euphotic zone, followed by vertical flux attenuation via mesopelagic processes3. Depth attenuation of the particulate organic carbon (POC) flux is modulated by multiple processes involving zooplankton and/or microbes4,5. Nevertheless, it continues to be mainly parameterized using an empirically derived relationship, the ‘Martin curve’6. The derived power-law exponent is the standard metric used to compare flux attenuation patterns across oceanic provinces7,8. Here we present in situ experimental findings from C-RESPIRE9, a dual particle interceptor and incubator deployed at multiple mesopelagic depths, measuring microbially mediated POC flux attenuation. We find that across six contrasting oceanic regimes, representing a 30-fold range in POC flux, degradation by particle-attached microbes comprised 7–29 per cent of flux attenuation, implying a more influential role for zooplankton in flux attenuation. Microbial remineralization, normalized to POC flux, ranged by 20-fold across sites and depths, with the lowest rates at high POC fluxes. Vertical trends, of up to threefold changes, were linked to strong temperature gradients at low-latitude sites. In contrast, temperature played a lesser role at mid- and high-latitude sites, where vertical trends may be set jointly by particle biochemistry, fragmentation and microbial ecophysiology. This deconstruction of the Martin curve reveals the underpinning mechanisms that drive microbially mediated POC flux attenuation across oceanic provinces.

Soil carbon dynamics in perennial biomass crops on marginally productive cropland in southern Canada

Predicting changes in soil organic carbon (SOC) in perennial biomass crops using process-based models provides a greater understanding of land management impacts on climate mitigation through long-term soil carbon sequestration. The objective of this study was to predict long-term SOC dynamics in different perennial biomass crops [miscanthus (Miscanthus giganteus L.), switchgrass (Panicum virgatum L.), willow (Salix miyabeana L.)] as compared to secondary regrowth vegetation (successional site) and a row crop system. The century model accurately predicted SOC when simulated values were compared to measured field data. Average SOC stocks over the 162-year simulation period to 20 cm, were highest in miscanthus (8521 g C m−2), followed by the successional site (6877 g C m−2), switchgrass (6480 g C m−2), willow (5448 g C m−2) and lowest in the row crop system (3995 g C m−2). Higher SOC stocks in the miscanthus than the successional site indicates that, despite frequent biomass harvest, perennial biomass crops can accumulate higher carbon in soil than when a marginally productive cropland is left to undergo secondary regrowth. However, this depends on the crop species, since the miscanthus was the only biomass crop that reached pre-cultivation (1911) SOC stock of 8288 g C m−2. Moreover, the perennial biomass crops enhanced SOC in the slow fraction, whereas row crops depleted SOC in this fraction. This indicates the vital contribution of perennial biomass crops in long-term SOC sequestration and their role in climate change mitigation, especially when grown on marginally productive croplands.

Effects of woodchip biochar on temperature sensitivity of greenhouse gas emissions in amended soils within a mountain vineyard

The utilization of biochar as a soil amendment holds promise for long-term carbon sequestration due to its elevated carbon content and persistent chemical structure. This characteristic has positioned biochar as a proposed nature-based solution for climate change mitigation. Nevertheless, the impact of biochar on soil greenhouse gas (GHG) emissions remains a subject of ongoing debate. In the present investigation, we evaluated the influence of conifer wood biochar on the fluxes of three GHGs, namely carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4), in a vineyard soil subjected to biochar-alone treatments (at rates of 25 and 50 t ha−1) and in combination with green waste compost (at a rate of 45 t ha−1). The experimental field was situated in northern Italy and was organized in a randomized block design. Soil GHG fluxes were monitored for two and a half years. Monthly flux measurements were conducted using a high-resolution multi-gas analyzer for 24 hours. Fluxes were, therefore, correlated with soil temperature to assess the influence of treatments on the sensitivity of GHG emissions to this pivotal environmental parameter. The findings demonstrated diminished temperature sensitivity in the initial experimental year across all GHG fluxes in soils amended with biochar and biochar-compost combination, in contrast to treatments lacking biochar (i.e., control and compost-alone treatments). Notably, the attenuation was most pronounced for N2O emissions, suggesting a potential role of biochar in mitigating the release of this gas. However, this effect did not persist in the second and third years of the experiment. Overall, biochar significantly contributed to a reduction in N2O fluxes and an increase in CO2 fluxes, but the effect was limited and temporary. Furthermore, biochar had no impact on CH4 fluxes. The discerned fluctuation in the impact of biochar over time can be attributed to the processes of biochar aging and/or the interannual variability in soil moisture.

Carbon Sequestration in Agricultural Soil: Technique and their Efficiency

Developing technologies to slow the rate of increase in the atmospheric concentration of carbon dioxide (CO2) from annual emissions, such as energy, process industries, land-use change, and soil cultivation, is a major challenge of the twenty-first century. Severe ecological and economic disruptions result from the erratic changes in climate systems caused by the skyrocketing amounts of atmospheric CO2 and other greenhouse gases (GHGs). Through the process of carbon sequestration, net greenhouse gas emissions can be reduced, hence mitigating climate change. Both biotic and abiotic variables can contribute to the long-term sequestration of carbon, or carbon stabilisation. The compilation of past and present methods for sequestering soil organic carbon is crucial, considering the need of measuring and tracking soil carbon at the point, field, regional, and ecosystem levels. This study attempts to provide an overview of the literature about the function of various agricultural management techniques for sequestering carbon and the ways in which they help to stabilize atmospheric carbon.

Impact of increased fishing on long-term sequestration of carbon by cephalopods

Fish and other metazoans play a major role in long-term sequestration of carbon in the oceans through the biological carbon pump1. Recent studies estimate that fish can release about 1,200 to 1,500 million metric tons of carbon per year (MtC year-1) in the oceans through feces production, respiration, and deadfalls, with mesopelagic fish playing a major role1,2. This carbon remains sequestered (stored) in the ocean for a period that largely depends on the depth at which it is released. Cephalopods (squid, octopus, and cuttlefish) have the potential to sequester carbon more effectively than fish because they grow on average five times faster than fish3,4 and they die after reproducing at an early age4,5 (usually 1-2 years), after which their carcasses sink rapidly to the sea floor6. Deadfall of carcasses is particularly important for long-term sequestration because it rapidly transports carbon to depths where residence times are longest1,6. We estimate that cephalopod carcasses transfer 11-22 MtC to the seafloor globally. While cephalopods represent less than 5% of global fisheries catch7, fishing extirpates about 0.36 MtC year-1 of cephalopod carbon that could otherwise have sunk to the seafloor, about half as much as that of fishing large fish8.

Life Cycle Assessment of Wheat Straw Pyrolysis with Volatile Fractions Chemical Looping Combustion

Among the approaches to facilitating negative CO2 emissions is biochar production. Biochar is generated in the pyrolysis of certain biomasses. In the pyrolysis process, carbon in the biomass is turned into a solid, porous, carbon-rich, and stable material that can be captured from the soil after a period of from a few decades to several centuries. In addition to this long-term carbon sequestration role, biochar is also beneficial for soil performance as it helps to restore soil fertility and improves the retention and diffusion of water and nutrients. This work presents a Life Cycle Assessment of different pyrolysis approaches for biochar production. Biomass pyrolysis is performed in a fixed-bed reactor, which operates at a mild temperature (550 °C). Biochar is obtained as solid product of the pyrolysis, but there are also liquid (bio-oil) and gaseous products (syngas). The pyrolysis gas is partly used to fulfil the energy demand of the pyrolysis process, which is highly endothermic. In the conventional approach, CO2 is produced during the combustion of syngas and emitted to the atmosphere. Another approach to facilitate CO2 capture and thus obtain more negative CO2 emissions in the pyrolysis process is burning syngas and bio-oil in a Chemical Looping Combustion unit. Life Cycle Assessment was performed of these approaches toward biomass pyrolysis to evaluate their environmental impact. The Chemical Looping Combustion approach significantly reduced the values of 7 of the 16 environmental impact indicators studied, along with the Global Warming Potential among them, it slightly increased the value of one indicator related to the use of fossil resources, and it maintained the values of the remaining 8 indicators. Environmental impact reduction occurs due to the avoidance of CO2 and NOx emissions with Chemical Looping Combustion. The CO2 balances of the different pyrolysis approaches with Chemical Looping Combustion configurations were compared with a base case, which constituted the direct combustion of wheat straw to obtain thermal energy. Direct biomass combustion for the production of 17.1 MJ of thermal energy had CO2 positive emissions of 0.165 kg. If the gaseous fraction was burned by Chemical Looping Combustion, CO2 was captured and the emissions became increasingly negative, until a value of −3.30 kg/17.1 MJ was generated. If bio-oil was also burned by this technology, the negative trend of CO2 emissions continued, until they reached a value of −3.66 kg.

Uranium-series and strontium isotope systematics in soil carbonates from dryland Critical Zones: Implications for soil inorganic carbon storage and transformation

Soil carbonates are dominantly present in dryland Critical Zone (CZ) and their formation could lead to important long-term carbon sequestration in arid to semiarid soils if the Ca ions were derived from silicate weathering or other non-carbonate sources. In managed CZ systems such as agricultural areas converted from natural drylands, irrigation has profound effects on the dryland CZ inorganic carbon storage, especially by modifying dissolution and precipitation dynamics of soil carbonates via controls of irrigation intensity and water chemistry, soil properties, and hydrological flow paths. These processes could lead to transformation of inorganic carbon in soils and groundwater aquifers underneath drylands. One key knowledge gap in studying soil carbonates from managed dryland CZ systems is to detect the formation of soil carbonates under irrigated conditions and distinguish them effectively from soil carbonates formed under natural conditions.Here, we explore the potential of using U-series and strontium isotopes to investigate the formation timescales and conditions of soil carbonates in both natural and managed dryland CZ in Chihuahua Desert of American Southwest. We obtained new U-series and Sr isotope ratios from mature stage V natural soil carbonates in the Jornada Basin of southern New Mexico and compared to previously published U-series and Sr isotope results for younger Jornada soil carbonates as well as irrigation impacted soil carbonates from the Rio Grande floodplains in west Texas. Specifically, we applied the U-series dating method (238U-234U-230Th) to estimate the timescales of soil carbonate formation under both natural climatic conditions and impacts of modern agriculture irrigation. In addition, we showed that the initial (234U/238U) activity ratios recorded in these soil carbonates reflect the systematic changes of soil infiltration rates in both natural and managed dryland CZ. We also utilized Sr isotope ratios (87Sr/86Sr) in soil carbonates, dust, and irrigation water to trace the Ca sources in soil carbonates from natural and managed dryland CZ. Soil carbonates from both settings were characterized by slightly radiogenic 87Sr/86Sr ratios, consistent with their potential of long-term carbon storage in drylands.U-series and Sr isotope systematics characterize important differences in soil carbonates from natural and managed dryland CZ with respect to their Ca sources, timescales of carbonate formation and transformation, and availability of soil moisture. Soil carbonates formed, accumulated, and transformed in natural drylands with long time scales but under irrigated arid agricultural areas its formation is significantly modified by irrigation and other agriculture practices, with implications for long- and short-term inorganic carbon sequestration in both systems of drylands.

CO2-induced micro-mechanical alterations of mudstone Caprock: Examples from the Mercia Mudstone Group, East Irish Sea Basin

Mechanical properties of caprock are important for subsurface energy sequestration as they determine the rock stability under the influence of external forces. Despite some advantages, there is a lack of knowledge regarding the interplay between rock texture and mechanical properties and their impact on the caprock stability within a short-term fluid-rock reaction period. Typical caprock samples from the Mercia Mudstone Group in the East Irish Sea Basin are studied in this work. X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) were used to identify minerology and local heterogeneity characteristics. Nanoindentation tests were conducted before and after CO2-brine-rock reaction to investigate the changes of micro-mechanical properties. After CO2-brine treatment, significant dolomite dissolution was observed in all samples (although dolomite content was only minor in some samples). In contrast, the micro-texture of other minerals showed no significant changes indicating minimal to no dissolution at the current resolution. The changes of mechanical properties are lower than expected, which are probably related to the impaired cementation induced by the retained water in pore throats and relatively rough surface after reaction. For long-term carbon sequestration, the layered depositional characteristics of different phases may not only form a barrier to prevent fluids leaking upward, but also complement each other in mechanical properties to maintain stability. This study improves the understanding of the effect of microscale structural and micro-mechanical changes on caprock stability in carbon sequestration related applications.

Estimation of Mariculture Carbon Sinks in China and Its Influencing Factors

The scientific assessment of mariculture carbon sinks is crucial to recognize its potential as a significant component of marine blue carbon in global climate change mitigation. Therefore, the objective of the research was to estimate the seaweed and shellfish mariculture carbon sink of different varieties in various sea areas. The paper emphasized the distinction between short-term carbon sequestration in seaweed and shellfish that can be removed and long-term carbon sequestration that is deposited. Methodologically, the evaluation was based on the carbon sequestration mechanism and systematic pathways in shellfish and seaweeds. Additionally, the carbon sequestration of shellfish and seaweed aquaculture over the last decade was evaluated by the carbon sink assessment model, and the reasons for the differences in the carbon sink capacity of mariculture in China’s coastal provinces were discussed by using the LMDI decomposition model. The results indicated the carbon sequestration of offshore seaweeds and shellfish mariculture in China was huge. From 2010 to 2020, offshore seaweed aquaculture in China amounted to 7.959 Mt C/a, while shellfish aquaculture contributed 33.542 Mt C/a to the carbon sinks. Sedimentary carbon sequestration by shellfish accounted for 51% of the total carbon sequestration in mariculture involving shellfish and seaweeds. Especially noteworthy is the sedimentary carbon sequestration by shellfish, which is an indispensable and crucial component of mariculture carbon sequestration estimation. It is concluded that improvements in farming efficiency exerted the greatest influence on the variations of the mariculture carbon sink, while adjustments in farming structure had a relatively minor impact in the case of little change in aquaculture yield. Enhancing farming efficiency emerges as a practical approach to bolstering the carbon sink potential of marine aquaculture fisheries in the future.

Factors controlling long-term carbon sequestration in the paleo-Yellow River delta: Implications for future delta management

River deltas have contributed to the regulation of atmospheric CO2 concentrations throughout Earth's history, but the capacity and controlling factors for deltas to serve as permanent carbon sinks over the long term are unclear. To address this issue, we carried out a high-resolution assessment of carbon burial in a well-dated sediment core (BXZK13) from the paleo-Yellow River delta on the west coast of the Bohai Bay. We found that the sedimentary environments in core BXZK13 since the Late Pleistocene (∼84 k cal yr B.P.) were divided into seven depositional units. The apparent mass accumulation rate (AMAR) of total organic carbon (TOC) and total inorganic carbon (TIC) varied significantly between depositional units. The average rates were highest in delta front deposits (TOC: 135 g m−2 yr−1, TIC: 333 g m−2 yr−1). Notably, the rates of carbon accumulation were dominated by TIC. The differences in the characteristics of TIC in the borehole compare to those of carbonates in Yellow River sediments and the positive correlation between TIC and Fe suggested that authigenic carbonate contributed significantly to long-term carbon burial in the delta. The apparent sedimentation rate (ASR) dominated the variations of the carbon AMAR, and the high clay and Fe content in the sediment enhanced TOC preservation and authigenic carbonate precipitation. Low concentrations of TIC were associated with several cold events, and there was a positive correlation between the chemical index of alteration and TIC. The implication was that climate may have affected the dynamics of inorganic carbon burial by regulating authigenic carbonate precipitation. These results enable a more thorough understanding of the factors that control long-term carbon sequestration, which is critical for both management purposes as well as assessment of the role of deltas in past and future climate change.

Carbon sequestration potential of plantation forests in New Zealand - no single tree species is universally best

BackgroundPlantation forests are a nature-based solution to sequester atmospheric carbon and, therefore, mitigate anthropogenic climate change. The choice of tree species for afforestation is subject to debate within New Zealand. Two key issues are whether to use (1) exotic plantation species versus indigenous forest species and (2) fast growing short-rotation species versus slower growing species. In addition, there is a lack of scientific knowledge about the carbon sequestration capabilities of different plantation tree species, which hinders the choice of species for optimal carbon sequestration. We contribute to this discussion by simulating carbon sequestration of five plantation forest species, Pinus radiata, Pseudotsuga menziesii, Eucalyptus fastigata, Sequoia sempervirens and Podocarpus totara, across three sites and two silvicultural regimes by using the 3-PG an ecophysiological model.ResultsThe model simulations showed that carbon sequestration potential varies among the species, sites and silvicultural regimes. Indigenous Podocarpus totara or exotic Sequoia sempervirens can provide plausible options for long-term carbon sequestration. In contrast, short term rapid carbon sequestration can be obtained by planting exotic Pinus radiata, Pseudotsuga menziesii and Eucalyptus fastigata.ConclusionNo single species was universally better at sequestering carbon on all sites we tested. In general, the results of this study suggest a robust framework for ranking and testing candidate afforestation species with regard to carbon sequestration potential at a given site. Hence, this study could help towards more efficient decision-making for carbon forestry.

Losses of native mineral-associated organic nitrogen through microbial mineralization and gaseous emissions induced by ammonium and nitrate addition

Atmospheric nitrogen (N) deposition has a significant impact on terrestrial N cycling. However, it has not been well understood how external inorganic N addition affects the dynamics of soil organic N (SON) that supports long-term plant productivity and carbon (C) sequestration. Here, we added six levels of ammonium (NH4+-N) or nitrate (NO3−-N) to a temperate grassland soil to reveal the mechanisms of SON responses to inorganic N addition. Results showed that the SON content decreased by 7% ± 2%–15% ± 1%, which corresponded with the increase in the abundance of functional gene related to SON mineralization (i.e., chiA) by both NH4+-N and NO3−-N. The inorganic N addition stimulated gaseous losses from the native soil N, leading to at least 6% ± 2%–12% ± 1% of the initial SON lost at different N addition levels (i.e., N-priming). However, the responses of nitrification- and denitrification-related functional genes to NH4+-N and NO3−-N treatments varied remarkably. The positive responses of nitrification- and denitrification-related functional genes (i.e., hao, nirS, and narG) to NO3−-N addition contributed to gaseous N losses. The negative responses of some denitrification-related functional genes (i.e., nirK, narG, and napA) to NH4+-N addition resulted in less gaseous N losses by NH4+-N compared with that by NO3−-N addition. Additionally, the release of H+ by NH4+-N addition may liberate organic matter from associated minerals, showing a decrease in mineral-associated organic matter (MAOM) but an increase in extractable Fe. These findings provide direct evidence that the addition of inorganic N results in a net native SON loss through microbe-mediated mineralization and gaseous emissions. The study also emphasizes the different mechanisms of reduced and oxidized N additions in affecting SON mineralization and gaseous N losses.

Carbon Sequestration Potential of Manure-Derived Hydrochar Aided by Secondary Stabilization.

Hydrothermal carbonization (HTC) is a process that produces a carbon-rich solid from wet organic materials through the application of heat and pressure. Carbonized solids, previously correlated to long-term soil stability, may be considered for carbon sequestration through incorporation into soil. Chars produced by pyrolysis are known for exceptional stability in soil, but pyrolysis is expensive when applied to wet biomass, such as manure. Chars produced from manure by HTC show considerably improved potential for carbon sequestration relative to untreated manure, although not as great as that of chars produced by pyrolysis. This study focuses on producing and evaluating chars by HTC paired with pyrolysis and different methods of chemical oxidation for long-term carbon sequestration in soil. It is shown that a two-step process of pyrolysis following HTC produces a char that outperforms those produced by either individual process (HTC or pyrolysis) in carbon yield, carbon content, and, more importantly, soil carbon sequestration potential. It was found that acid-catalyzed HTC followed by pyrolysis resulted in a char with a 13% increase in carbon yield, a 51% increase in carbon content, and an atomic O/C ratio 64% smaller than the char produced by conventional pyrolysis.

Species-Level Classification of Peatland Vegetation Using Ultra-High-Resolution UAV Imagery

Peatland restoration projects are being employed worldwide as a form of climate change mitigation due to their potential for long-term carbon sequestration. Monitoring these environments (e.g., cover of keystone species) is therefore essential to evaluate success. However, existing studies have rarely examined peatland vegetation at fine scales due to its strong spatial heterogeneity and seasonal canopy development. The present study collected centimetre-scale multispectral Uncrewed Aerial Vehicle (UAV) imagery with a Parrot Sequoia camera (2.8 cm resolution; Parrot Drones SAS, Paris, France) in a temperate peatland over a complete growing season. Supervised classification algorithms were used to map the vegetation at the single-species level, and the Maximum Likelihood classifier was found to perform best at the site level (69% overall accuracy). The classification accuracy increased with the spatial resolution of the input data, and a large reduction in accuracy was observed when employing imagery of >11 cm resolution. Finally, the most accurate classifications were produced using imagery collected during the peak (July–August) or early growing season (start of May). These findings suggest that despite the strong heterogeneity of peatlands, these environments can be mapped at the species level using UAVs. Such an approach would benefit studies estimating peatland carbon emissions or using the cover of keystone species to evaluate restoration projects.

Ecosystem CO2 Exchange and Its Environmental Regulation of a Restored Wetland in the Liaohe River Estuary

Coastal wetlands are important carbon sinks， and they contribute to reducing the effects of global warming. This study used the eddy covariance method to detect the CO2 flux in the restoration wetland of the Liaohe River estuary in 2021 and investigate the characteristics of ecosystem CO2 exchange and its environmental control factors. The aim was to assess the carbon source/sink capacity of salt marshes in the restored area and to provide data support and theoretical basis for evaluating the effectiveness of ecological restoration projects. The study revealed "U" curves in spring and autumn， "V" curves in summer， and horizontal lines in winter for the average daily variation curve of net ecosystem CO2 exchange （NEE） in the restored area. Its carbon sink efficiencies were -40.06， -63.62， 2.33， and 34.43 g·m-2 in the spring， summer， autumn， and winter， respectively. In the restored area， the daily cumulative variation in NEE was "V" shaped， and the monthly cumulative changes in NEE， ecosystem respiration （Reco）， and gross primary productivity （GPP） were obviously different. Photosynthetically active radiation （PAR） was an important regulation factor of daytime NEE in the restored area in 2021， and they displayed a rectangular hyperbolic relationship. PAR could explain 53% of the variation in the daytime NEE. Air temperature （Ta） was the main control factor of Reco，night， and there was an exponential relationship between them. When Ta < 5.5 ℃， the temperature sensitivity of ecosystem respiration （Q10） was 2.19， and Ta could explain 42% of the variation in the Reco，night； when Ta ≥ 5.5 ℃， the Q10 was 1.81， and Ta could explain 51% of the variation in the Reco，night. Additionally， there were significant linear negative correlations between NEE and both soil water content （SWC） and vapor pressure deficit （VPD）， whereas NEE was not significantly correlated with soil temperature （Ts） or relative humidity （RH）. In 2021， the restored wetland in the Liaohe River estuary acted as a CO2 sink， and the total net carbon sequestration was -66.89 g·m-2. The restored salt plays a role as an important carbon sink and has long-term carbon sequestration potential.

Effects of fresh-salt water interaction on spatial variations of soil organic carbon in reed wetland of Yellow River Estuary.

Estuarine wetlands exhibit significant interaction between fresh and salt water, with long-term carbon sequestration capability. We set up 60 sampling sites in the reed wetlands of the fresh-salt water interaction zone of the Yellow River Estuary, covering four different zones of the weak-intensity fresh-salt water interaction zone (WIZ), medium-intensity fresh-salt water interaction zone (MIZ), high-intensity interaction fresh-salt water zone (HIZ) and strong-intensity fresh-salt water interaction zone (SIZ). We investigated how fresh-salt water interaction affected the spatial variation of soil organic carbon (SOC) storage. The results showed that the area of reed wetland accounted for 17.8% of the total area of the fresh-salt water interaction zone the Yellow River Estuary, which mainly distributed in the WIZ and MIZ. The SOC content of reed wetland in the fresh-salt water interaction zone ranged from 1.09 to 3.65 g·kg-1, the SOC density was between 1.85-5.84 kg·m-2, and the SOC storage was (17.32±3.64)×104 t. The SOC content and SOC density decreased with increasing fresh-salt water interaction. There were significant differences in surface SOC content between different subzones of the fresh-salt water interaction zone. The surface SOC content decreased significantly with the increases of fresh-salt water interaction intensity. SOC density was positively correlated with SOC, TN, NH4+-N, and biomass, but negatively correlated with salt ions, soil bulk density, pH, and EC. SOC storage in the 0-30 cm soil layer accounted for 50.9%-64.2% of that in the 0-60 cm soil layer, while SOC storage in the 0-60 cm soil layer occupied 19.1%-37.7% of that in the 0-400 cm soil layer. The results could provide a scientific basis for accurately evaluating SOC storage of estuarine wetlands, improving carbon sink function and wetland management.

Nanoscale profiling of the relationship between in-situ organic matter roughness, adhesion, and wettability under ScCO2 based on contact mechanics

Understanding the interface and wetting phenomenon between CO2 and shale in the reservoir environment plays a key role in realizing efficient shale gas development and long-term stable carbon sequestration in the formation. However, previous studies on the interaction between CO2 and reservoir rocks focused on macro-scale and batch experiments of inorganic minerals, and the mechanism at the nanoscale, especially the micro-wetting behavior of the organic interface, remains unclear and needs to be investigated urgently. In this paper, based on the atomic force microscopy (AFM) tapping mode and the AM-FM viscoelastic mapping mode, the in-situ organic matter (OM) morphology and the change of the relationship between the adhesion force and the interfacial wettability under carbon dioxide immersion are investigated, respectively, and the carbon dioxide wettability behavior at the organic interfaces is deeply revealed in the nanoscale. Among them, the contact angle is obtained by the adhesion work combined with the Johnson-Kendall-Roberts (JKR) adhesive contact theory. The results show that the surface roughness of the OM keeps increasing with the ScCO2 immersion time, while the surface adhesion keeps decreasing at the same time. Although the contact angle decreases continuously with immersion time, the OM is always hydrophobic, i.e., CO2 wetting. It can be seen that the surface roughness has a negative correlation with the contact angle, which keeps decreasing with decreasing adhesion. This indicates that ScCO2 immersion expands the gas adsorption sites and at the same time reduces the interfacial energy of the OM slightly, which is extremely beneficial for CO2 to enhance shale gas recovery and to implement long-term stable carbon geologic sequestration. Therefore, this study reveals the mechanism of the carbon sequestration process at the organic interface in depth within the nanoscale for the first time, which provides a new perspective to deeply understand the long-term adsorption and capture variation process of OM in deep reservoirs.