ObjectiveThis study aims to compare the effect of question-answer and Kahoot method reinforcement on nursing students' success and motivation levels. Materials and methodsThis quasi-experiment study was carried out with 65 students studying at the nursing department at a state university in Turkey. The research data were obtained between November 2019 and February 2020. The study sample was divided into two groups as question-answer and Kahoot. In the first stage of the research, 8 h of training were given to all 65 students on the subject “Infection Control and Precautions,” applying the method of teaching by presentation. Shortly after the training, the Kahoot group was reinforced on the Kahoot platform and the Question-answer group in PowerPoint, using the same questions for 30 min. In the second stage of the study, one day after the training, “Student Information Form” and “Instructional Materials Motivation Scale” were filled out, and “Achievement Test 1” was implemented. In the third stage, “Achievement Test 2” was executed two months after the training to test longer term knowledge retention. ResultsThe Kahoot group students had a score of 66.63 ± 11.02 in the “Achievement Test 1” and 67.76 ± 15.62 in the question-answer group. It was determined that the total score average of the students in the Kahoot group “Achievement Test 2” was 51.25 ± 13.42, and the students in the question-answer group were 47.03 ± 12.61. Accordingly, there was no statistically significant difference between the “Achievement Test 1” and “Achievement Test 2” mean scores of the students in the question-answer and Kahoot group (p > 0.05). The total score average of the question-answer group for “Instructional Materials Motivation Scale” was 127.88 ± 13.64, while the Kahoot group was 126.28 ± 16.88. It was determined that there was no statistically significant relationship between the “Achievement Test 1”, “Achievement Test 2” and the “Instructional Materials Motivation Scale” total score and sub-dimensions of the students in the Question-Answer group and Kahoot group (p > 0.05). ConclusionIn the study analyzing the effect of reinforcement by the question-answer or Kahoot method on nursing students' success and motivation levels, it was concluded that no method was superior.