Problem. Doctor of Biological Sciences, Professor Svitlana Oleksandrivna Ignatova dedicated her entire life to several scientific institutions in Odesa: V. Ye. Tairov Institute of Viticulture and Winemaking (1964–1970), All-Union Plant Breeding and Genetics Institute (1970–1991), the Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation (1991–1999 and 2012–2018), and the South Plant Biotechnological Center of the Ukrainian Academy of Agrarian Sciences (2000–2012). Her life and work were inextricably linked to the development of agricultural biotechnology in Ukraine. Aim. The purpose of our research was to honor the memory of Professor S. O. Ignatova, to highlight the main biographical data and the results of her scientific and pedagogical activities. Results. S. O. Ignatova fruitfully worked on theoretical and applied problems of agricultural crops biotechnology and was a pioneer in the use of achievements in Ukraine regarding the successful application in the selection process of the source material obtained by in vitro methods of tissue, organ and cell culture. Under the leadership of Svitlana Oleksandrivna, the principles and complete technology of accelerated production of linear varieties of barley and wheat with the use of haploproducers were developed. The barley varieties – Odessky 115th and Prairie, which were sown on a large area in Ukraine, were created using this technology. The great attention of S. O. Ignatova was devoted to the training of scientific personnel (graduate students, interns and students). Conclusions. S. O. Ignatova’s scientific legacy was a long-term fundamental research in the biotechnology of major agricultural crops, which was a significant contribution to the development of advanced areas of Ukrainian and world science. Svitlana Oleksandrivna was the author of more than 300 scientific works, including monographs, patents, scientific and methodological manuals, methodological recommendations, author’s certificates and publications in leading scientific journals. The life story of S. O. Ignatova is continued by her students, remembering the bright image of their professor – an energetic, determined and inspired woman.