In intensive agricultural systems, efficient nutrient use is necessary for high crop yields as well as for sustainable environmental management. Recent studies in temperate regions indicate that non-exchangeable NH4 +-N (NEA), which is fixed in clay minerals, may affect crop productivity and soil N dynamics more than previously thought. To estimate the quantity and plant availability of NEA in Swedish soils, ryegrass (Lolium perenne) was grown in a pot experiment using 18 soils that were collected (0–20 cm depth) from two long-term agricultural experiment series at five locations. Initial NEA, total N and soil K contents were measured, as well as NEA content 56, 112 and 168 days after planting of ryegrass. The results show that the soils (0–20 cm) contained 21–217 mg NEA kg−1 sieved soil (5–300 kg NEA ha−1) estimated as corresponding to 0.1–5.1% of the total soil N. Long-term application of farmyard manure (FYM) did not increase contents of soil NEA. Long-term application of K fertilizer increased soil contents of AL-extractable K, but there was no significant correlation with NEA content. Concurrent with ryegrass growth, NEA content decreased on average by 16% between day 0 and day 112, indicating that NEA was released from the soil and taken up by the plants.
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