Loline alkaloids are present in meadow fescue containing the endophyte (Neotyphodium uncinatum Gams, Petrini and Schmidt) (Clavicipitacae). Root, crown and shoot loline alkaloid concentrations in 10 selected lines from meadow fescue ecotypes are reported for the first time, from a Canterbury farm during 2004–05. The concentrations of four loline alkaloid derivatives, N-formyl loline (NFL), N-acetyl loline (NAL), N-acetyl norloline (NANL) and N-methyl loline (NML), in these lines (each line represented by one genotype) were determined at four harvest dates during late spring, late summer, and early and late autumn. There were marked differences in loline alkaloid concentration between lines and seasons. Maximum shoot loline concentration was recorded in summer (up to 2860 µg/g in Fp408). Root loline alkaloid concentration was substantially higher in late autumn (up to 790 µg/g in Fp408) and the shoot concentration correspondingly lower than in spring, summer and early autumn suggesting loline alkaloid transportation from shoots to roots. In the lines tested at each of the four harvest dates in spring, summer, and autumn, the root, crown, and shoot alkaloid concentration with minor exceptions was NFL > NAL > NANL > NML.