Estimation of Octanol-Water Partition Coefficients and Correlation with Dermal Absorption for Several Polyhalogenated Aromatic Hydrocarbons. Jackson, J. A., Diliberto, J. J., and Birnbaum, L. S. (1993). Fundam. Appl. Toxicol. 21, 334-344.n-Octanol-Water partition coefficients (Kow) were experimentally estimated for; 2,3,7,8-tetrachloro and 2,3,7,8-tetrabromodibenzo-p-dioxin; 2,3,7,8-tetrachloro-, 2,3,7,8-tetrabromo-, and 1,2,7,8-tetrabromodibenzofuran; 1,2,3,7,8-pentachloro-, 1,2,3,7,8-pentabromo-, 2,3,4,7,8 pentachloro-, and 2,3,4,7,8-pentabromodibenzofuran; 1,2,4,6,8,9-hexachlorodibenzofuran (HxCDF), and 3,4,3′,4′-tetrachlorobiphenyl (3,4,3′,4′TCB). The method involved correlation of literature Kow with reverse-phase high-performance liquid chromotography (RP-HPLC) retention time for a series of 23 calibration standards and estimation of Kow for test compounds from measured RP-HPLC retention. Retention times for all standards and test chemicals were measured in duplicate on the same octadecasilane (C18) reverse-phase column with an isocratic 85:15 methanol:water mobile phase; solutes were detected by uv absorbance (254 nm). Literature log Kow values used for the calibration standards had been measured exclusively by the generator column method. Log Kow estimates for the test compounds in the present study ranged from 5.45 for 3,4,3′,4′-TCB to 6.81 for HxCDF. Kow estimates were then plotted against laboratory data for the in vivo 3-day dermal absorption of single equimolar doses (200 pmol and 20 nmol, or ∼1 nmol/kg and ∼0.1 μmol/kg) of selected test compounds in male F344 rats. Strong inverse correlations were found between octanol-water partition coefficient estimates and single-dose dermal absorption for most of the compounds studied. In addition, RP-HPLC retention time itself appeared to be as equally suited as Kow to such correlations with dermal absorption. The structure-activity relationships suggested in this study were sought in order to explain observed differences in the dermal absorption of polyhalogenated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners differing in number, position, and/or type (Cl or Br) of halogen substituents. Moreover, these results should be of predictive value in the risk assessment of dermal exposure to polychlorinated dibenzop-dioxins, dibenzofurans, biphenyls, and their brominated analogues.