Locomotion Parameters Research Articles

Chronic exposure to low concentration of diflubenzuron and pyriproxyfen induces brain oxidative stress and inflammation and alters locomotion in zebrafish (Danio rerio)

Diflubenzuron (DFB1) and pyriproxyfen (PPF) are pesticides widely used in agriculture and urban environments to control insect actions. The aim of this study was to evaluate the effects of chronic 30-day exposure to DFB (0.025 and 0.125 mg/L) and PPF (0.379 and 0.758 mg/L) on the behavior of juvenile zebrafish (Danio rerio). Fish were exposed to insecticides from early stage (4 h post fertilization) to 30 days post fertilization (dpf). At 45 dpf, fish were evaluated in the novel tank test, social behavior test, and mirror aggressive test. Brain gene expression related to oxidative stress and inflammation was also evaluated. DFB reduced locomotor parameters in the novel tank and aggression tests, while it induced to hyperactivity in the social behavior test. PPF reduced anxiety-like behavior, measured by the time spent in risky areas of the novel tank, and reduced aggression against the mirror image. There was a significant reduction in mRNA levels of the nfe2l2 gene (∼0.54 fold downregulated) and increase in levels of cat (PPF ∼1.8 fold change), gsr (PPF ∼1.5 fold change), gpx1a (PPF ∼1.6 and DFB 1.1 fold change), tnf-α (PPF 1.9 and DFB 2.2 fold change), and il-6 (PPF ∼1.2 and DFB 2.3 fold change). These endpoints are indicative of the threatening effects of insecticides to aquatic organisms and the need for alternative methods to control pests by using less harmful and safer substances for animal and human well-being, as well as for the environment.

Analysis of the influence of brake pipe pressure gradient on heavy haul combined train and optimisation of operation strategy

In this paper, based on the theory of gas flow, the action logic of the control valve, and the principle of multi-rigid-body dynamics, a simulation model of the 20,000 t heavy haul combined train is established to investigate the influence of brake pipe pressure gradient on the braking performance, longitudinal dynamics, braking and release characteristics of trains. Utilizing real railway line conditions, locomotive and vehicle parameters, and train operation monitoring equipment (LKJ) record data of Shuozhou-Huanghua Railway, the cyclic braking condition of the train on the long and steep downhill is simulated, and the control strategy of reducing the coupler force during the cyclic braking is proposed. The results indicate that a larger brake pipe pressure gradient before braking leads to weaker braking capability, and higher coupler forces during braking, but smaller coupler forces during release. As the brake pipe pressure gradient before braking increases, braking synchronicity decreases, while release synchronicity slightly improves. By adjusting the locomotive’s electric braking force during the cyclic braking, it is possible to appropriately increase the brake pipe pressure gradient before braking, effectively reducing coupler forces during release and reducing the number of braking cycles, thus lowering the operational difficulty of the train.

Jurassic–Cretaceous boundary theropod tracks from the slope of the Fallen Feng, Chicheng, China: review and new observations

ABSTRACT The Tuchengzi Formation represents a Jurassic – Cretaceous boundary regional lithological unit that bridges the temporal gap between the Yanliao biota and the Jehol biota. Tetrapod tracks represent the most significant vertebrate fossil records in the formation, and can be employed to supplement the Late Jurassic fauna and palaeoecological data from northern China. Here, we present a re-analysis of the theropod tracks from the Luofenggou site in northern Hebei Province, utilising an additional 3D model and a locomotory analysis to provide further insights into the ecological context. A morphological analysis indicates that these tracks are more similar to Grallator than to the previously proposed Therangospodus. The latter is diagnosed by coalesced, elongate, oval digital pads lacking a separation in distinct phalangeal pads, whereas these are clearly defined in the presented Tuchengzi material. They can therefore be assigned to the widespread G. ssatoi ichnospecies from the Tuchengzi Formation, although they are longer and more variable in size than other G. ssatoi. A general consideration of speed-related locomotive parameters, trackway morphology, and superposition allows for the speculation of the existence of cooperative or predatory relationships between theropod trackmakers that were differentiated by size or stages of ontogeny, but had similar foot structures.

Contextual fear conditioning in zebrafish: Influence of different shock frequencies, context, and pharmacological modulation on behavior

Contextual fear conditioning is a protocol used to assess associative learning across species, including fish. Here, our goal was to expand the analysis of behavioral parameters that may reflect aversive behaviors in a contextual fear conditioning protocol using adult zebrafish (Danio rerio) and to verify how such parameters can be modulated. First, we analyzed the influence of an aversive stimulus (3 mild electric shocks for 5 s each at frequencies of 10, 100 or 1000 Hz) on fish behavior, and their ability to elicit fear responses in the absence of shock during a test session. To confirm whether the aversive responses are context-dependent, behaviors were also measured in a different experimental environment in a test session. Furthermore, we investigated the effects of dizocilpine (MK-801, 2 mg/kg, i.p.) on fear-related responses. Zebrafish showed significant changes in baseline activity immediately after shock exposure in the training session, in which 100 Hz induced robust contextual fear responses during the test session. Importantly, when introduced to a different environment, animals exposed to the aversive stimulus did not show any differences in locomotion and immobility-related parameters. MK-801 administered after the training session reduced fear responses during the test, indicating that glutamate NMDA-receptors play a key role in the consolidation of contextual fear-related memory in zebrafish. In conclusion, by further exploring fear-related behaviors in a contextual fear conditioning task, we show the effects of different shock frequencies and confirm the importance of context on aversive responses for associative learning in zebrafish. Additionally, our data support the use of zebrafish in contextual fear conditioning tasks, as well as for advancing pharmacological studies related to associative learning in translational neurobehavioral research.

The Autophagic Activator GHF-201 Can Alleviate Pathology in a Mouse Model and in Patient Fibroblasts of Type III Glycogenosis.

Glycogen storage disease type III (GSDIII) is a hereditary glycogenosis caused by deficiency of the glycogen debranching enzyme (GDE), an enzyme, encoded by Agl, enabling glycogen degradation by catalyzing alpha-1,4-oligosaccharide side chain transfer and alpha-1,6-glucose cleavage. GDE deficiency causes accumulation of phosphorylase-limited dextrin, leading to liver disorder followed by fatal myopathy. Here, we tested the capacity of the new autophagosomal activator GHF-201 to alleviate disease burden by clearing pathogenic glycogen surcharge in the GSDIII mouse model Agl-/-. We used open field, grip strength, and rotarod tests for evaluating GHF-201's effects on locomotion, a biochemistry panel to quantify hematological biomarkers, indirect calorimetry to quantify in vivo metabolism, transmission electron microscopy to quantify glycogen in muscle, and fibroblast image analysis to determine cellular features affected by GHF-201. GHF-201 was able to improve all locomotion parameters and partially reversed hypoglycemia, hyperlipidemia and liver and muscle malfunction in Agl-/- mice. Treated mice burnt carbohydrates more efficiently and showed significant improvement of aberrant ultrastructural muscle features. In GSDIII patient fibroblasts, GHF-201 restored mitochondrial membrane polarization and corrected lysosomal swelling. In conclusion, GHF-201 is a viable candidate for treating GSDIII as it recovered a wide range of its pathologies in vivo, in vitro, and ex vivo.

Effect of an antidepressant on aquatic ecosystems in the presence of microplastics: A mesocosm study

Emerging pollutants, such as pharmaceuticals and microplastics have become a pressing concern due to their widespread presence and potential impacts on ecological systems. To assess the ecosystem-level effects of these pollutants within a multi-stressor context, we simulated real-world conditions by exposing a near-natural multi-trophic aquatic food web to a gradient of environmentally relevant concentrations of fluoxetine and microplastics in large mesocosms over a period of more than three months. We measured the biomass and abundance of different trophic groups, as well as ecological functions such as nutrient availability and decomposition rate. To explore the mechanisms underlying potential community and ecosystem-level effects, we also performed behavioral assays focusing on locomotion parameters as a response variable in three species: Daphnia magna (zooplankton prey), Chaoborus flavicans larvae (invertebrate pelagic predator of zooplankton) and Asellus aquaticus (benthic macroinvertebrate), using water from the mesocosms. Our mesocosm results demonstrate that presence of microplastics governs the response in phytoplankton biomass, with a weak non-monotonic dose-response relationship due to the interaction between microplastics and fluoxetine. However, exposure to fluoxetine evoked a strong non-monotonic dose-response in zooplankton abundance and microbial decomposition rate of plant material. In the behavioral assays, the locomotion of zooplankton prey D. magna showed a similar non-monotonic response primarily induced by fluoxetine. Its predator C. flavicans, however, showed a significant non-monotonic response governed by both microplastics and fluoxetine. The behavior of the decomposer A. aquaticus significantly decreased at higher fluoxetine concentrations, potentially leading to reduced decomposition rates near the sediment. Our study demonstrates that effects observed upon short-term exposure result in more pronounced ecosystem-level effects following chronic exposure.

In-vivo evaluation of neuroprotective effect of Chinese plant calendula officinalis Linn. Flower Extract against Aluminium chloride-induced Alzheimer's in Wistar rats

ObjectiveTo investigate the neuroprotective properties of Calendula Officinalis Linn. flower extract (COE) against mild cognitive decline and cognitive dysfunction induced by aluminium chloride (AlCl3) in experimental rats. MethodologyCOE (100, 200, and 300 mg/kg p.o.) was given once daily following the treatment of AlCl3 (100 mg/kg p.o.) to male Wistar rats for 20 days. On the 1st, 10th, and 20th days, the walking track test, locomotor activity, and object recognition test were used as behaviour characteristics to assess spatial and non-spatial memory. On the 21st day, animals were sacrificed and brains removed for biochemical and histopathological analysis. ResultsCalendula officinalis Linn. extract significantly attenuated the AlCl3-induced alterations in body weight, and motor coordination such as locomotion, stride length, and object recognition parameters. COE also reduced oxidative stress by increasing GSH, catalase, SOD, and decreasing TBARS levels. Calendula officinalis Linn. extract high dose showed most prominent therapeutic effects. P < 0.05 and mean ± SD were used to compare mean values with normal control, disease control, and high doses of Calendula officinalis Linn. extract. DiscussionBased on our study, oxidative stress has been proposed as a mechanism for AlCl3-induced neurotoxicity. We conclude that Calendula officinalis Linn. flower extract substantially decreased oxidative stress and behavioural alterations in rats treated with aluminium chloride. Calendula officinalis flowers may provide significant neuroprotective effects, potentially improving memory and learning abilities. Calendula officinalis Linn flower extract contains anti-inflammatory and antioxidant effects that might be responsible for its neuroprotective properties.

Effects of Environmental Enrichment on Exposure to Human-Relevant Mixtures of Endocrine Disrupting Chemicals in Zebrafish.

Fish models used for chemical exposure in toxicological studies are normally kept in barren tanks without any structural environmental enrichment. Here, we tested the combined effects of environmental enrichment and exposure to two mixtures of endocrine disrupting chemicals (EDCs) in zebrafish. Firstly, we assessed whether developmental exposure to an EDC mixture (MIX G1) combined with rearing the fish in an enriched environment influenced behaviour later in life. This was evaluated using locomotion tracking one month after exposure, showing a significant interaction effect between enrichment and the MIX G1 exposure on the measured locomotion parameters. After three months, we assessed behaviour using custom-made behaviour tanks, and found that enrichment influenced swimming activity. Control fish from the enriched environment were more active than control fish from the barren environment. Secondly, we exposed adult zebrafish to a separate EDC mixture (MIX G0) after rearing them in a barren or enriched environment. Behaviour and hepatic mRNA expression for thyroid-related genes were assessed. There was a significant interaction effect between exposure and enrichment on swimming activity and an effect of environment on latency to approach the group of conspecifics, where enriched fish took more time to approach the group, possibly indicating that they were less anxious. Hepatic gene expression of a thyroid-related gene (thrb) was significantly affected by EDC exposure, while enrichment had no discernible impact on the expression of the measured genes. In conclusion, environmental enrichment is important to consider when studying the effects of EDCs in fish.

Pharmacological Evidence of Eugenia Brasiliensis Leaves in a Reserpine-Induced Fibromyalgia Model: Antinociceptive, Emotional, Anti-Inflammatory, and Neurotrophic Effects.

Fibromyalgia (FM) is a painful chronic condition that significantly impacts the quality of life, posing challenges for clinical management. Given the difficulty of understanding the pathophysiology and finding new therapeutics, this study explored the effects of a medicinal plant, E. brasiliensis, in an FM model induced by reserpine in Swiss mice. Animals were treated with saline 0.9% (vehicle), duloxetine 10 mg/kg (positive control), or hydroalcoholic extract of E. brasiliensis leaves 300 mg/kg (HEEb). Nociceptive parameters, as well as locomotion, motor coordination, strength, anxiety, and depressive-like behaviors, were evaluated for 10 days. After that, the brain and blood were collected for further analysis of cytokines (interleukin 1? and interleukin 6), brain-derived neurotrophic factor (BDNF), and the immunocontents of total and phosphorylated Tropomyosin receptor kinase B (TrkB). The results demonstrated that the acute and prolonged treatment with HEEb was able to reduce both mechanical and thermal nociception. It was also possible to observe an increase in the strength, without changing locomotion and motor coordination parameters. Interestingly, treatment with HEEb reduces anxious and depressive-like behaviors. Finally, we observed a reduction in inflammatory cytokines in the hippocampus of animals treated with HEEb, while an increase in BDNF was observed in the prefrontal cortex (PFC). However, no alterations related to total and phosphorylated TrkB receptor expression were found. Our study demonstrated the antinociceptive and emotional effects of HEEb in mice, possibly acting on neuroinflammatory and neurotrophic mechanisms. These data provide initial evidence about the E. brasiliensis potential for treating chronic pain.

Differences in behaviour, facial expressions and locomotion between positive anticipation and frustration in horses

Animal welfare is a result of accumulated negative and positive emotions. Therefore, it is important to limit the former and promote the latter. Doing so requires a precise identification of these emotions. The aim of this study was to characterize the behaviours, facial expressions and locomotor parameters of 21 horses in two conditions with opposite valence, presumed to induce positive anticipation and frustration. In the positive anticipation situation, the horse was led to a bucket of food, knowing that they would be allowed to eat it. In the frustration situation, experimenters indicated to the horse that food was available without allowing them to eat it. In the positive anticipation situation, horses exhibited a lower neck position with the ears forward and upper lip advanced and went faster by increasing their stride frequency accompanied by increased global locomotor activity. In the frustration situation, horses exhibited a higher neck position with the ears backward or to the side, accompanied by ear movements and eye blinks, and interacted more with the experimenters. This study describes new possible indicators of positive anticipations and frustration in horses.

Induction of Paraquat-Mediated Parkinsonian Phenotype in Zebrafish.

Paraquat (PQ) is a well-known neurotoxin closely associated with neurodegenerative Parkinson's disease (PD). Zebrafish are utilized as a model for PD research because of their well-defined neuropathology and locomotor behavior. Here, we highlight protocols for inducing PD using PQ and analyzing locomotor activity in adult zebrafish. Basic Protocol 1 details the treatment of adult male zebrafish with 60 mg/kg PQ via intraperitoneal injection to induce a PD-like phenotype, followed by the steps to perform a locomotor assay. Basic Protocol 2 provides step-by-step guidance for processing the acquired videos in ToxTrac software to understand the locomotor parameters of 0.9% saline- and 60 mg/kg PQ-injected adult zebrafish. The simplicity of the treatment strategy, low-cost video acquisition setup, and free video processing make these protocols accessible without prior experience. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Development of Parkinson's disease features in adult zebrafish Basic Protocol 2: ToxTrac analysis for locomotor assay.

Alterations in Rumination, Eating, Drinking and Locomotion Behavior in Dairy Cows Affected by Subclinical Ketosis and Subclinical Acidosis

This study delves into the effects of subclinical ketosis (SCK) and subclinical acidosis (SCA) on various parameters related to dairy cow rumination, eating, drinking and locomotion behavior. The research hypothesized that these subclinical metabolic disorders could affect behaviors such as rumination, feeding, and locomotion. A total of 320 dairy cows, with a focus on those in their second or subsequent lactation, producing an average of 12,000 kg/year milk in their previous lactation, were examined. These cows were classified into three groups: those with SCK, those with SCA, and healthy cows. The health status of the cows was determined based on the milk fat–protein ratio, blood beta-hydroxybutyrate, and the results of clinical examinations performed by a veterinarian. The data collected during the study included parameters from the RumiWatch sensors. The results revealed significant differences between the cows affected by SCK and the healthy cows, with reductions observed in the rumination time (17.47%) and various eating and chewing behaviors. These changes indicated that SCK had a substantial impact on the cows’ behavior. In the context of SCA, the study found significant reductions in Eating Time 2 (ET2) of 36.84% when compared to the healthy cows. Additionally, Eating Chews 2 (EC2) exhibited a significant reduction in the SCA group, with an average of 312.06 units (±17.93), compared to the healthy group’s average of 504.20 units (±18.87). These findings emphasize that SCA influences feeding behaviors and chewing activity, which can have implications for nutrient intake and overall cow health. The study also highlights the considerable impact of SCK on locomotion parameters, as the cows with SCK exhibited a 27.36% reduction in the walking time levels. These cows also displayed reductions in the Walking Time (WT), Other Activity Time (OAT), and Activity Change (AC). In conclusion, this research underscores the critical need for advanced strategies to prevent and manage subclinical metabolic disorders within the dairy farming industry. The study findings have far-reaching implications for enhancing the well-being and performance of dairy cattle. Effective management practices and detection methods are essential to mitigate the impact of SCK and SCA on dairy cow health and productivity, ultimately benefiting the dairy farming sector.

A contact parameter estimation method for multi-modal robot locomotion on deformable granular terrains

AbstractIn this paper, we consider the problem of contact parameters (slippage and sinkage) estimation for multi-modal robot locomotion on granular terrains. To describe the contact events in the same framework for robots operated at different modes (e.g., wheel, leg), we propose a unified description of contact parameters for multi-modal robots. We also provide a parameter estimation method for multi-modal robots based on CNN and DWT (discrete wavelet transformation) techniques and verify its effectiveness over different types of granular terrains. Besides motion modes, this paper also considers the influence of slope angles and the robot’s handing angles over contact parameters. Through comparison and analysis of the prediction results, our method can not only effectively predict the contact parameters of multi-modal robot locomotion on a granular medium (better than $96\%$ accuracy) but also achieves the same or better performance when compared to other (direct) contact measurement methods designed for individual motion modes, that is, single-modal robots such as quadruped robots and mars rovers. Our proposed unified contact parameter estimation method can be useful for studying the interaction mechanics between multi-modal robots and granular terrains as well as terrain classification tasks due to its superior sensitivity which is analyzed in the experiments.

Locomotor activity as an effective measure of the severity of inflammatory arthritis in a mouse model.

Mouse models are valuable in preclinical studies of inflammatory arthritis. However, current methods for measuring disease severity or responses to treatment are not optimal. In this study a smart cage system using multiple sensors to measure locomotor activity was evaluated in the K/BxN serum transfer model of inflammatory arthritis. Arthritis was induced in C57BL/6 mice with injections of K/BxN serum. Clinical index and ankle thickness were measured for 14 days. Locomotor activity was measured in smart cages for 23 h periods on Days 0, 7, and 13. The same measurements were taken in mice consuming diets supplemented or not with fish oil to evaluate a preventative treatment. Initiation, peak and resolution phases of disease could be measured with the smart cages. Locomotor activity including speed, travel distance, number of active movements and rear movements were all significantly lower on Days 7-8 of illness (peak) compared to Days 0 and 13-14 (resolution) (one-way repeated measures analyses, p<0.05). The clinical index and ankle thickness measurements did not capture differences between dietary groups. Significantly increased activity was measured in most of the locomotor parameters in the fish oil group compared to the control mice at both Days 8 and 14 (2-way repeated measures ANOVA, p<0.05). The measurement of locomotor activity provided a more detailed evaluation of the impact of inflammatory arthritis on animal well-being and mobility than that provided by measuring clinical index and ankle thickness, and could be a valuable tool in preclinical studies of inflammatory arthritis.

Understanding the differential impacts of two antidepressants on locomotion of freshwater snails (Lymnaea stagnalis)

There is growing evidence of negative impacts of antidepressants on behavior of aquatic non-target organisms. Accurate environmental risk assessment requires an understanding of whether antidepressants with similar modes of action have consistent negative impacts. Here, we tested the effect of acute exposure to two antidepressants, fluoxetine and venlafaxine (0–50 µg/L), on the behavior of non-target organism, i.e., freshwater pond snail, Lymnaea stagnalis. As compounds interact with chemical cues in the aquatic ecosystems, we also tested whether the effects altered in the presence of bile extract containing 5α-cyprinol sulfate (5α-CPS), a characterized kairomone of a natural predator, common carp (Cyprinus carpio). Behavior was studied using automated tracking and analysis of various locomotion parameters of L. stagnalis. Our results suggest that there are differences in the effects on locomotion upon exposure to venlafaxine and fluoxetine. We found strong evidence for a non-monotonic dose response on venlafaxine exposure, whereas fluoxetine only showed weak evidence of altered locomotion for a specific concentration. Combined exposure to compounds and 5α-CPS reduced the intensity of effects observed in the absence of 5α-CPS, possibly due to reduced bioavailability of the compounds. The results highlight the need for acknowledging different mechanisms of action among antidepressants while investigating their environmental risks. In addition, our results underline the importance of reporting non-significant effects and acknowledging individual variation in behavior for environmental risk assessment.Graphical

Sensorimotor transformation underlying odor-modulated locomotion in walking Drosophila

Most real-world behaviors – such as odor-guided locomotion - are performed with incomplete information. Activity in olfactory receptor neuron (ORN) classes provides information about odor identity but not the location of its source. In this study, we investigate the sensorimotor transformation that relates ORN activation to locomotion changes in Drosophila by optogenetically activating different combinations of ORN classes and measuring the resulting changes in locomotion. Three features describe this sensorimotor transformation: First, locomotion depends on both the instantaneous firing frequency (f) and its change (df); the two together serve as a short-term memory that allows the fly to adapt its motor program to sensory context automatically. Second, the mapping between (f, df) and locomotor parameters such as speed or curvature is distinct for each pattern of activated ORNs. Finally, the sensorimotor mapping changes with time after odor exposure, allowing information integration over a longer timescale.

Functioning and Gene Expression of Adenosine A1 Receptor During Zebrafish (Danio rerio) Development.

The A1 adenosine receptor is the most widely expressed P1 receptor in vertebrates, performing inhibitory tone of the nervous system. Increased levels of adenosine are crucial to promote tissue protection in threatening situations, such as convulsion and hypoxia. Zebrafish is an established model organism for studies on health and disease. In this study, we evaluated the functionality of A1 adenosine receptor through development of zebrafish (6-7-day-, 3-, 8-, and 24-month-old), assessing: (I) the effects of the agonist N6-cyclopenthyladenosine (CPA) over locomotor parameters, (II) the anticonvulsant properties of CPA and adenosine per se in the pentylenetetrazol-induced seizure, and (III) the gene expression of adora1b through development. CPA promoted decreased distance traveled in the highest concentrations/doses tested (larvae: 75 to 500 μM; adults: 20 mg.kg-1), altered mean velocity (larvae: 50-500 μM; adults: 20 mg.kg-1) and time in the bottom zone of apparatus (adults: decrease in 20 mg.kg-1). Adenosine increased the latency of the larvae to reach stage II at 5 and 10 μM. CPA anticonvulsant effect against convulsive stage II was reached at 75 μM, although it decreased basal locomotor activity in larvae. For adults, CPA 10 mg.kg-1 was effective as anticonvulsant without locomotory effects. Adenosine had minor anticonvulsant effects in the concentration tested (larvae: 5 and 10 μM). The level of gene expression of adora1b was stable in brain from adult animals (8- and 24-month-old animals). These results suggest that zebrafish has similar responses to CPA as mammals. To avoid confounding factors, such as locomotor effects, during any brain function investigation using A1 adenosine receptor as a target, the concentration below 75 μM or below the dose of 20 mg.kg-1 of CPA is ideal for zebrafish at larval and adult stages, respectively.

THE INFLUENCE OF PHYSICAL THERAPY ON INDICATORS OF LOCOMOTIVE SYNDROME IN ELDERLY PERSONS WITH OSTEOARTHRITIS OF THE KNEE AND OBESITY

Aim: to assess the effectiveness of the developed physical therapy program on locomotive syndrome parameters in elderly people with osteoarthritis of the knee and obesity.&#x0D; Material and methods. 93 elderly people were examined. The control group consisted of 31 people without osteoarthritis of the joints, with no obesity. The main group 1 consisted of 33 people who received rehabilitation according to the general principles of osteoarthritis correction. Representatives of the main group 2 (31 people) were engaged in a physical therapy program using functional training on the «Prosedos» platform, therapeutic exercises, Proprioceptive Neuromuscular Facilitation; massage, kinesiological taping, nutritional correction, patient education. The effectiveness of the program was evaluated according to the dynamics of the Short Physical Performance Battery, Functional Gait Assessment, Tinetti-test (Performance-Oriented Mobility Assessment), 25-question Geriatric Locomotive Function Scale.&#x0D; Results. Elderly patients with osteoarthritis of the knee and obesity were found to have impaired balance (Short Physical Performance Battery), altered gait stereotype (Functional Gait Assessment), risk of falling when performing activities of daily living (Tinetti-test) at the level of locomotive syndrome II degree (25- question Geriatric Locomotive Function Scale). The developed program of physical therapy in persons of the main group 2 revealed a statistically significantly better effect on the components of the locomotive syndrome compared to the initial examination in all studied parameters (p&lt;0.05). Elderly patients who underwent rehabilitation according to the standard program for the correction of osteoarthritis achieved a statistically significant improvement compared to the initial state according to the studied parameters (p&lt;0.05), but less pronounced compared to the tested program.&#x0D; Conclusions. Elderly patients with osteoarthritis of the knee joints and obesity need to develop physical therapy programs taking into account and correcting the specifics of each condition, the presence of static and dynamic balance disorders and the risk of falling, which will increase the overall effectiveness of rehabilitation measures.

Diet affects the growth and behavior of Argiope bruennichi spiders and correlates with the species richness of their vertically and horizontally transmitted tissue-resident bacteria

The ability to spread and build quality webs is crucial to spider survival. These abilities may differ in response to dietary adjustments and changes in assemblages of tissue-resident bacteria, including vertically transmitted endosymbiotic taxa, particularly when expanding outside the native ranges. We measured the physical and behavioral parameters of the invasive wasp spider Argiope bruennichi subjected to four types of diets, including protein-rich and lipid-rich diets. We used 16S rDNA sequencing to evaluate vertically and horizontally transmitted tissue-resident bacteria, including endosymbionts, in the cephalothorax and legs of the tested spiders. The diet composition affected the body weight and carapace length as well as the locomotor parameters but not the orb-weaving abilities. Dietary treatments led to changes in the diversity and operational taxonomic unit (OTU) richness of facultative and obligate tissue endosymbionts, with Wolbachia and Rickettsia having higher relative abundance in spiders fed a lipid-rich diet. The higher OTU richness of facultative and obligate endosymbionts was associated with better locomotor performance. The other tissue-resident microbes were dominated by Firmicutes; the dominance and diversity of tissue-resident microbiomes also differed among spiders treated with different diets. The highest alpha diversity of tissue-resident microbes was present in spiders fed the standard diet, whereas all other diets led to a decrease in alpha diversity and various changes at the level of individual genera and OTUs. In conclusion, the tissues of A. bruennichi host tremendously diverse assemblages of bacteria, including obligate or facultative endosymbionts, which are sensitive to differences in diet composition and affect the locomotion of their hosts.

Audiogenic Seizures and Social Deficits: No Aggravation Found in Krushinsky-Molodkina Rats.

Epilepsy or epileptic syndromes affect more than 70 million people, often comorbid with autism spectrum disorders (ASD). Seizures are concerned as a factor for social regression in ASD. A stepwise experimental approach to this problem requires an animal model to provoke seizures and monitor subsequent behavior. We used rats of the Krushinsky-Molodkina (KM) strain as a validated inbred genetic model for human temporal lobe epilepsy, with recently described social deficiency and hypolocomotion. Generalized tonic-clonic seizures in KM rats are sound-triggered, thus being controlled events in drug-naïve animals. We studied whether seizure experience would aggravate contact deficits in these animals. Locomotor and contact parameters were registered in "the elevated plus maze", "socially enriched open field", and "social novelty/social preference tests" before and after sound-provoked seizures. The triple seizure provocations minimally affected the contact behavior. The lack of social drive in KM rats was not accompanied by a submissive phenotype, as tested in "the tube dominance test", but featured with a poor contact repertoire. Here, we confirmed our previous findings on social deficits in KM rats. The contact deficiency was dissociated from hypolocomotion and anxiety and did not correlate with seizure experience. It was established that experience of rare, generalized tonic-clonic convulsions did not lead to an impending regress in contact motivation, as seen in an animal model of genetic epilepsy and comorbid social deficiency. One of the oldest animal models for epilepsy has a translational potential to study mechanisms of social behavioral deficits in future neurophysiological and pharmacological research.