Although considerable progress has been made within the last decade in experimental hydrodynamic analyses of aquatic locomotion using two-dimensional digital particle image velocimetry (two-dimensional DPIV), data have been limited to simultaneous calculation of two out of the three flow velocity variables: downstream (U), vertical (V) and lateral (W). Here, we present the first biological application of stereo-DPIV, an engineering technique that allows simultaneous calculation of U, V and W velocity vectors. We quantified the wakes of rainbow trout (Oncorhynchus mykiss, 16.5-21.5 cm total body length, BL), swimming steadily in a recirculating flow tank at a slow cruising speed of 1.2 BL s(-1). These data extend the comparative basis of current hydromechanical data on the wakes of free-swimming fishes to the salmoniforms and are used to test current hypotheses of fin function by calculations of mechanical performance and Froude efficiency. Stereo-DPIV wake images showed three-dimensional views of oscillating jet flows high in velocity relative to free-stream values. These jet flows are consistent with the central momentum jet flows through the cores of shed vortex rings that have been previously viewed for caudal fin swimmers using two-dimensional DPIV. The magnitude and direction of U, V and W flows in these jets were determined over a time series of 6-8 consecutive strokes by each of four fish. Although the fish swam at the same relative speed, the absolute magnitudes of U, V and W were dependent on individual because of body size variation. Vertical flows were small in magnitude (<1 cm s(-1)) and variable in direction, indicating limited and variable vertical force production during slow, steady, forward swimming. Thus, in contrast to previous data from sunfish (Lepomis macrochirus) and mackerel (Scomber japonicus), the trout homocercal caudal fin does not appear to generate consistent vertical forces during steady swimming. U flows were of the order of 3-6 cm s(-1); lateral flows were typically strongest, with W magnitudes of 4-6 cm s(-1). Such strong lateral flows have also been shown for more derived euteleosts with homocercal caudal fins. The ratios of the magnitudes of wake flow, U/(U+V+W), which is a flow equivalent to mechanical performance, were also dependent on individual and ranged from 0.32 to 0.45, a range similar to the range of mechanical performance values previously determined using standard two-dimensional DPIV methods for caudal fin locomotion by more derived euteleosts. Strong lateral jet flow appears to be a general feature of caudal fin locomotion by teleosts and may reflect the nature of undulatory propulsion as a posteriorly propagated wave of bending. Froude efficiency (eta(p)) was independent of individual; mean eta(p) was 0.74, which is similar to previous findings for trout.