Location Of Region Research Articles

Cascade spatial and channel-wise multifusion network with criss cross augmentation for corneal segmentation and reconstruction

High-quality 3D corneal reconstruction from AS-OCT images has demonstrated significant potential in computer-aided diagnosis, enabling comprehensive observation of corneal thickness, precise assessment of morphological characteristics, as well as location and quantification of keratitis-affected regions. However, it faces two main challenges: (1) prevalent medical image segmentation networks often struggle to accurately process low-contrast corneal regions, which is a vital pre-processing step for 3D corneal reconstruction, and (2) there are no reconstruction methods that can be directly applied to AS-OCT sequences with 180-degree scanning. To combat these, we propose CSCM-CCA-Net, a simple yet efficient network for accurate corneal segmentation. This network incorporates two key techniques: cascade spatial and channel-wise multifusion (CSCM), which captures intricate contextual interdependencies and effectively extracts low-contrast and obscure corneal features; and criss cross augmentation (CCA), which enhances shape-preserved feature representation to improve segmentation accuracy. Based on the obtained corneal segmentation results, we reconstruct the 3D volume data and generate a topographic map of corneal thickness through corneal image alignment. Additionally, we design a transfer function based on the analysis of intensity histogram and gradient histogram to explore more internal cues for better visualization results. Experimental results on CORNEA benchmark demonstrate the impressive performance of our proposed method in terms of both corneal segmentation and 3D reconstruction. Furthermore, we compare CSCM-CCA-Net with state-of-the-art medical image segmentation approaches using three challenging medical fundus segmentation datasets (DRIVE, CHASEDB1, FIVES), highlighting its superiority in terms of segmentation accuracy. The code and models will be made available at https://github.com/qianguiping/CSCM-CCA-Net.

First Karyotypic Insights into Potamotrygon schroederi Fernández-Yépez, 1958: Association of Different Classes of Repetitive DNA

Introduction: Currently, there are 38 valid species of freshwater stingrays, and these belong to the subfamily Potamotrygoninae. However, cytogenetic information about this group is limited, with studies mainly using classical techniques, Giemsa, and C-banding. Methods: In this study, we used classical and molecular cytogenetic techniques – mapping of 18S and 5S rDNA and simple sequence repeats (SSRs) – in order to investigate the karyotypic composition of Potamotrygon schroederi and reveal the karyoevolutionary trends of this group. Results: The species presented 2n = 66 chromosomes with 18m + 12sm + 16st + 20a, heterochromatic blocks distributed in the centromeric regions of all the chromosomes, and terminal blocks in the q arm of pairs 2 and 3. Mapping of 18S rDNA regions revealed multiple clusters on pairs 2 and 7 and a homolog of pair 24. The 5S rDNA region was found in the pericentromeric portion of the subtelocentric pair 16. Furthermore, dinucleotide SSRs sequences were found in the centromeric and terminal regions of different chromosomal pairs, with preferential accumulation in pair 17. In addition, we identified conspicuous blocks of (GATA)n and (GACA)n sequences colocalized with the 5S rDNA (pair 16). Conclusion: In general, this study corroborates the general trend of a reduction in 2n in the species of Potamotrygoninae subfamily. Moreover, we found that the location of rDNA regions is very similar among Potamotrygon species, and the SSRs accumulation in the second subtelocentric pair (17) seems to be a common trait in this genus.

The lived experience of rural churches pastorally caring for farmers and graziers: a practical theology conversation

ABSTRACT This article presents a conversation between a grazier, two farmers (one ordained), a rurally based chaplain, and a social worker regarding rural church engagements. Inspired by practical theology, these collaborators address the research question: From the perception of the co-authors, how does the Anglican Church of Australia engage with farmers and graziers in rural, regional and remote locations? Utilising the co-operative inquiry methodology, this empirical research reminds us of the strengths of the rural Anglican church despite its fragility. It highlights that the church is an important and unique space where people in a community can explore existential crises and theological questions. Based on the authors’ experiences and reflections, the rural Anglican church can contextualise the gospel and give members a sense of belonging while engaging with the beauty and transitions of rural living. It can also provide practical and pastoral care for members transitioning through family tragedy, anxiety or the potentially traumatic experience of farm succession planning.

Developing a simple and efficient modeling solution for predicting key phenological stages of table grapes in a non-traditional viticulture zone in south Asia.

Phenological shifts are one of the most visible signs of climatic variability and change in the biosphere. However, modeling plant phenological responses has always been a key challenge due to climatic variability and plant adaptation. Grapevine is a phenologically sensitive crop and, thus, its developmental stages are affected by the increase in temperature. The goal of this study was to develop a temperature-based grapevine phenology model (GPM) for predicting key developmental stages for different table grape cultivars for a non-traditional viticulture zone in south Asia. Experiments were conducted in two vineyards at two locations (Chakwal and Islamabad) in the Pothawar region of Pakistan during the 2019 and 2020 growing seasons for four cultivars including Perlette, King's Ruby, Sugraone and NARC Black. Detailed phenological observations were obtained starting in January until harvest of the grapes. The Mitscherlich monomolecular equation was used to develop the phenology model for table grapes. There was a strong non-linear correlation between the Eichhorn and Lorenz phenological (ELP) scale and growing degree days (GDD) for all cultivars with coefficient of determinations (R2) ranging from 0.90 to 0.94. The results for model development indicated that GPM was able to predict phenological stages with high skill scores, i.e., a root mean square (RMSE) of 2.14 to 2.78 and mean absolute error (MAE) of 1.86 to 2.26 days. The prediction variability of the model for the onset timings of phenological stages was up to 3 days. The results also reveal that the phenology model based on GDD approach provides an efficient planning tool for viticulture industry in different grape growing regions. The proposed methodology, being a simpler one, can be easily applied to other regions and cultivars as a predictor for grapevine phenology.

Risk Factors Associated with Postoperative Cerebrospinal Fluid Leaks After Intrathecal Drug Delivery System and an External Pump Implantation in Cancer Patients: A Retrospective Study.

To determine risk factors associated with postoperative cerebrospinal fluid leaks (CSFLs) after intrathecal drug delivery system (IDDS) and external pump implantation. The clinical data of 248 patients with advanced cancer who underwent IDDS implantation from January 2021 to December 2022 at the Department of Pain Medicine at the Hunan Cancer Hospital were retrospectively reviewed. Information regarding age, gender, height, weight, body mass index (BMI), tumour type, albumin levels, haemoglobin levels, history of diabetes and pre- and postoperative anti-tumour therapy was collected and analysed. Postoperative CSFLs occurred in 7 of 231 patients (3.30%). Statistical analysis indicated that gender, age, height, weight, BMI, tumour type, albumin levels, haemoglobin levels, history of diabetes, pre- and postoperative chemotherapy, pre- and postoperative radiotherapy, preoperative immunotherapy and postoperative targeted therapy were not independent factors for CSFLs. Preoperative targeted therapy [odds ratio (OR): 16.64; 95% confidence interval (CI): 1.42, 195.56; P = 0.01] and postoperative immunotherapy (OR: 13.38; 95% CI: 1.60, 111.65; P = 0.017) were factors associated with an increased postoperative CSFL rate. Of the two locations where CSFLs can occur, the back (puncture site of catheter, n = 4) and the hypochondriac region (location of infusion port implanted, n = 3), back CSFLs occurred earlier than in the hypochondriac region (18.25 ± 6.45 vs 115 ± 62.02 days, P = 0.032). Based on the data from our study, the timing of preoperative targeted therapy and postoperative immunotherapy should be considered to prevent the occurrence of CSFLs in cancer pain patients who have an IDDS and external pump.

Mycobacterium avium subsp. paratuberculosis in Wild Boar (Sus scrofa) in Portugal.

Paratuberculosis, or Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The agent was also found in wild mammals such as wild boar (Sus scrofa); however, the role of wild mammals in the epidemiology of MAP is unclear. During the research period, 941 free-ranging wild boar (S. scrofa) legally hunted in two locations in the central-eastern region of Portugal were examined. Ninety-seven wild boars exhibited one or more gross lesions and were tested for the presence of Mycobacterium avium subsp. paratuberculosis using acid-fast staining, mycobacterial culture, polymerase chain reaction (PCR), and histopathological examination. Forty-five animals (46.4%, 95% CI: 36.5-56.3%) were identified as infected, as indicated by positive results in culture and/or PCR. The findings revealed that the most significant risk factor was being a juvenile compared to yearlings and adults (OR = 10.2, 95% CI: 2.2-48.0). Based on our results, 37.9% (n = 11) of the infected animals were considered suitable for human consumption. Our findings offer novel insights into mycobacterial infections in wild boar populations in Portugal and suggest that wild boar could be a source of human infection if zoonotic potential is considered.

Acceptance of offshore wind farm in Southwest Sardinia in Italy. Do regional energy policies matter?

Purpose The social acceptance of wind energy is increasingly conditioning the Italian Government and regions to authorize the construction of these plants. The proposal of offshore wind farm in the south-west of Sardinia has raised many perplexities both from the marine environmental point of view and from concern about increasing the electricity production in a region that already exports electricity to the peninsula. The purpose of this study is to evaluate what are the factors that most affect the coastal residents’ acceptability of an offshore wind farm. Design/methodology/approach The data is based on a Sardinia-wide in-person survey with about 512 participants in the period between May and June 2023. Respondents were selected randomly from five different locations in Carloforte region. Multiple regression analysis investigates the factors that influenced acceptability to construction and development of offshore wind power plant. Four independent variables were selected in the regression models such as (i) interest towards wind energy, (ii) attitude towards renewable energy production, (iii) perceived regional energy policy and (iv) attitude towards offshore wind farm. The dependent variables are the rates of coastal local residents’ acceptance of offshore wind farm for respondents in five coastal towns. Findings Fishermen and summer tourism operators’ respondents expressed significant reluctance to accept the new project because they perceive a strong risk of environmental impact on both tuna fishing and marine recreational activities in the vicinity. The distance between the turbines at sea and local residents along the coast and surrounding small islands does not have an influence to accept wind energy. The energy policy of the Region of Sardinia, which is perceived by respondents as an expansive energy policy in the production of electricity that exceeds their needs, not being accompanied by a scenario of reducing that from fossil fuels, is the variable that led about 70% of respondents to express a negative opinion towards this project. Research limitations/implications A potential problem associated with the survey is that local response produced sample selection bias. The proportion of respondents with secondary education and no school certificate is quite high. It is possible that respondents who provided valid questionnaires were more low-educated and therefore, the sample may be biased towards lower-educated people. While this does not invalidate the results of this study, it is important to note that the sample was on average less educated than the overall population in Italy. In addition, people with lower incomes were more likely to complete the questionnaires; the results are more representative of a portion of the population with incomes below the national average. Practical implications Results showed how coastal communities are in favour of wind energy within their island, but not within a marine protected area that is considered unique for its beauty and marine ecosystem. Wind energy developers should, before submitting a proposal for an offshore wind farm project, consider among other things the long approval time and understand the activities carried out by local communities and their attachment to those places. Politicians and developers should develop a coherent energy transition policy based on a long-term vision of zero emissions, because according to the findings of this study, it was the regional energy policy that is the most controversial reason for residents to reject the project. Originality/value The contribution of this study is to fill a part of the research gap linking to ongoing energy transitions. In particular, this study analysed for the first time in Italy the acceptability level of an offshore renewable energy project considering the environmental implications and risks in the fragile marine ecosystem of Carloforte waters. This study also made it possible to analyse the impact of the regional energy policy on the acceptability of residents towards the development of offshore wind farms, which is scarcely discussed in the literature.

Health-related quality of life among people with diabetes: A cross-sectional study in Hail region, Saudi Arabia ​.

Diabetes Mellitus is a serious and expanding health problem, together with the issues of health- related quality of life (HRQoL). This further puts pressure on the government to allocate more funds for public healthcare. This study was devised to evaluate the health-related quality of life of people living with diabetes in Hail region of Saudi Arabia. This cross-sectional research was carried out at eight locations in the Hail region of Saudi Arabia between 21st March-20th May 2022 using the adapted version of the Euro QoL-5 dimension (EQ-5D-3L) questionnaire. A multistage random sample approach was used to choose the diabetes clinics, and data collectors approached the participants in the waiting areas to collect the information. The data were analyzed using logistic regression analysis, Mann-Whitney test, and Kruskal-Wallis tests in IBM SPSS statistics 21.0. The mean HRQoL score was 0.71±0.21 with a visual analog score of 68.4±16.2. Despite having much higher levels of quality of life in terms of self-care (85.8%), regular activity (73.8%) and anxiety (71.8%), nearly one half of the people reported moderate pain or discomfort, and more than one third reported having moderate mobility issues. In general, the quality of life for women was poorer than for men. Individuals with diabetes who were unmarried, young, educated, financially secure, and taking only oral medication had much improved HRQoL. The Euro QoL of people with diabetes patients were significantly influenced by gender, marital status, age, education, employment and treatment modality (p-values < 0.05), whereas only treatment modality had a significant impact on the patients' visual analogue measures (p-values < 0.05). The HRQoL of people with diabetes in Hail region was moderate in general, with pain and mobility issues being particularly prevalent. Gender, marital status, age, education, employment and type of medication therapy are significant predictors of HRQoL of patients with diabetes. Hence, planning and programs to enhance the HRQoL of people with diabetes, especially women is recommended.

Physical Modelling Of The Wave Field Around An Array Of Centrally Controlled Wave Energy Converters

Stepped revetments have shown to be effective in limiting wave overtopping and wave run-up compared to sloped revetments. However, literature on wave impact pressures and comprehensive design guidelines for these structures is scarce. Laboratory experiments support establishing design recommendations. So far, studies for wave impacts at stepped revetments were mainly performed at small scales. Results from these tests are likely to be subjected to scale effects and therefore inaccurately replicate the wave-structure interaction at full scale. This study quantifies scale effects of wave impact characteristics for design cases based on a comparison to small-scale tests (Kerpen et al., 2018). Full-scale flume experiments were studied with a slope of 1:3 and uniform step heights of 0.17 m and 0.50 m in the Large Wave Flume (GWK) in Hannover, Germany. Horizontal and vertical wave impacts were measured at 15 locations in the plunging region of the revetment for a range of wave steepnesses (). The results show that previous small-scale tests underestimate design wave impact pressures by a factor of up to 7.7. Impact loadings occur considerably faster than at small scale with relative peak rising times decreasing by a factor of up to 5.6. Prediction formulae are derived for the vertical distribution of horizontal impact pressures (Figure 1b) as well as for temporal characteristics of these pressures at stepped revetments.

Regional-specific calibration enables application of computational evidence for clinical classification of 5′ cis-regulatory variants in Mendelian disease

To date, clinical genetic testing for Mendelian disease variants has focused heavily on exonic coding and intronic gene regions. This multi-step study was undertaken to provide an evidence base for selecting and applying computational approaches for use in clinical classification of 5' cis-regulatory region variants. Curated datasets of clinically reported disease-causing 5' cis-regulatory region variants and variants from matched genomic regions in population controls were used to calibrate six bioinformatic tools as predictors of variant pathogenicity. Likelihood ratio estimates were aligned to code weights following ClinGen recommendations for application of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology (ACMG/AMP) classification scheme. Considering code assignment across all reference dataset variants, performance was best for CADD (81.2%) and REMM (81.5%). Optimized thresholds provided moderate evidence toward pathogenicity (CADD, REMM) and moderate (CADD) or supporting (REMM) evidence against pathogenicity. Both sensitivity and specificity of prediction were improved when further categorizing variants based on location in an EPDnew-defined promoter region. Combining predictions (CADD, REMM, and location in a promoter region) increased specificity at the expense of sensitivity. Importantly, the optimal CADD thresholds for assigning ACMG/AMP codes PP3 (≥10) and BP4 (≤8) were vastly different from recommendations for protein-coding variants (PP3≥25.3; BP4≤22.7); CADD <22.7 would incorrectly assign BP4 for >90% of reported disease-causing cis-regulatory region variants. Our results demonstrate the need to consider a tiered approach and tailored score thresholds to optimize bioinformatic impact prediction for clinical classification of 5' cis-regulatory region variants.

Zoogeographical regions in the Atlantic Forest: patterns and potential drivers

AbstractAimTo delineate present‐day zoogeographical regions of terrestrial vertebrates (frogs, lizards, snakes, birds and non‐volant mammals) in the Atlantic Forest. Within each taxonomic group, we examine the relative importance of abrupt climatic transitions, orographic barriers, past climate change and rivers in shaping zoogeographical boundaries.LocationSouth America's Atlantic Forest.MethodsWe applied a network‐based method to delineate zoogeographical regions, using distribution data (range maps) for 455 species of frogs, 103 lizards, 220 snakes, 917 birds and 202 non‐volant mammals, in 50 × 50 km grid cells. We used hierarchical generalized linear mixed‐effects models to test environmental predictors associated with zoogeographical boundaries. Finally, we intersected the bioregion maps delineated for each group to identify general patterns across all vertebrates.ResultsWe identified four zoogeographical regions for birds and snakes, and five for frogs, lizards and non‐volant mammals. Depending on the group, contemporary and past climate conditions, elevation variation and/or rivers were associated with zoogeographical boundaries. The combined maps indicate that the Atlantic Forest retains four spatially cohesive zoogeographical regions based on present‐day distribution of vertebrates.Main conclusionsCross‐taxon congruence indicates that the geographical and environmental characteristics of the Atlantic Forest have a strong influence on the location of zoogeographical regions for vertebrates. In contrast, transition zones appear to be associated with the spatial distribution of life history traits of each group, potentially explaining the observed differences in the number of bioregions across groups and the position of zoogeographical boundaries. This work paves the way for further research into the evolutionary assembly of the Atlantic Forest's zoogeographical regions and may help inform conservation priorities for maintaining their distinctive faunas.

THRIVING IN MOTION – YOUTH MOVES: MOVEMENT FOR YOUTH MENTAL HEALTH PROGRAM

CASE SETTING A Western Australia community exercise initiative supporting vulnerable youth experiencing poor mental health. Led by professionals and peers, our program offers a non-judgmental space for exercise and education, addressing discrimination, isolation, and service access barriers. Addressing a lack of physical activity options for at-risk groups, the program targets individuals affected or at risk of mental health disorders, recognizing that a quarter of youth face such challenges. TREATMENT An 8-week evidence-based program integrating mixed movement and education in alternative or education support settings. Tailored for at risk youth, the goal is to directly improve mental and physical health literacy, and social outcomes of participants through diverse exercise opportunities to manage and maintain thriving mental health. Specifically, we delivered and evaluated the feasibility of the program for at risk youth. RESULTS Since 2022, Thriving has delivered 43 8-week programs directly impacting 855 vulnerable youth in various metro (13) and regional (2) Western Australian locations, totalling 20, 860 exercise therapy hours. Forty-two collaborative partnerships and 15 staff have been involved in delivery. On average 85% of participants reported a score higher than 3/5 for program enjoyment, 80% of participants reported a score higher than 3/5 for motivation to return. REFLECTIONS/LEARNINGS Despite the abundance of community-based exercise and sporting organizations, a significant gap exists in their ability to address the needs of vulnerable youth, stemming from insufficient specialized skills and training. Informed by the achievements and insights of prior collaborations and projects, our program focuses on establishing inclusive activity environments for youth in low participation groups. Building on successful community relationships, we strive to encourage disengaged youth, allies, friends and families by offering tailored opportunities and pathways for meaningful exercise participation.

Energetic Neutral Atoms Detected in the 2022 February 15 Solar Energetic Particle Event

Energetic neutral atoms (ENAs) are expected to be produced near the Sun during large solar energetic particle (SEP) events. However, their detection by SEP instruments near 1 au has been limited. The clearest reported measurement has been from the Solar Terrestrial Relations Observatory (STEREO) during the 2006 December 5 SEP event. Additional evidence of ENAs has been found through reanalysis of observations by the Solar Anomalous and Magnetospheric Particle Explorer obtained near the equator in low Earth orbit and associated with several large X-ray flares and fast coronal mass ejections (CMEs). Here we describe another detection of ENAs from the STEREO Low Energy Telescope associated with the large 2022 February 15 SEP event. Given the timing and spectrum of the ENAs and the location of the source region (behind the east limb from STEREO’s viewpoint), these ENAs are most likely a result of acceleration by a CME-driven shock when the CME was at approximately 2–3 R S. The possibility of a postflare loop origin is considered unlikely.

Interplay between α-synuclein and parkin genes: Insights of Parkinson's disease.

Parkinson's disease (PD) is a complex and debilitating neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the substantia nigra. The pathogenesis of PD is intimately linked to the roles of two key molecular players, α-synuclein (α-syn) and Parkin. Understanding the intricate interplay between α-syn and Parkin is essential for unravelling the molecular underpinnings of PD. Their roles in synaptic function and protein quality control underscore their significance in neuronal health. Dysregulation of these processes, as seen in PD, highlights the potential for targeted therapeutic strategies aimed at restoring normal protein homeostasis and mitigating neurodegeneration. Investigating the connections between α-syn, Parkin, and various pathological mechanisms provides insights into the complex web of factors contributing to PD pathogenesis and offers hope for the development of more effective treatments for this devastating neurological disorder. The present compilation provides an overview of their structures, regional and cellular locations, associations, physiological functions, and pathological roles in the context of PD.

Transcriptomic Investigation of the Virus Spectrum Carried by Midges in Border Areas of Yunnan Province.

Yunnan province in China shares its borders with three neighboring countries: Myanmar, Vietnam, and Laos. The region is characterized by a diverse climate and is known to be a suitable habitat for various arthropods, including midges which are notorious for transmitting diseases which pose significant health burdens affecting both human and animal health. A total of 431,100 midges were collected from 15 different locations in the border region of Yunnan province from 2015 to 2020. These midges were divided into 37 groups according to the collection year and sampling site. These 37 groups of midges were then homogenized to extract nucleic acid. Metatranscriptomics were used to analyze their viromes. Based on the obtained cytochrome C oxidase I gene (COI) sequences, three genera were identified, including one species of Forcipomyia, one species of Dasyhelea, and twenty-five species of Culicoides. We identified a total of 3199 viruses in five orders and 12 families, including 1305 single-stranded positive-stranded RNA viruses (+ssRNA) in two orders and seven families, 175 single-stranded negative-stranded RNA viruses (-ssRNA) in two orders and one family, and 1719 double-stranded RNA viruses in five families. Six arboviruses of economic importance were identified, namely Banna virus (BAV), Japanese encephalitis virus (JEV), Akabane virus (AKV), Bluetongue virus (BTV), Tibetan circovirus (TIBOV), and Epizootic hemorrhagic disease virus (EHDV), all of which are capable, to varying extents, of causing disease in humans and/or animals. The survey sites in this study basically covered the current distribution area of midges in Yunnan province, which helps to predict the geographic expansion of midge species. The complexity and diversity of the viral spectrum carried by midges identified in the study calls for more in-depth research, which can be utilized to monitor arthropod vectors and to predict the emergence and spread of zoonoses and animal epidemics, which is of great significance for the control of vector-borne diseases.

Antivenom surveillance: An audit of antivenom stock within South Australia.

Antivenoms are important emergency medications to be held within Australia, particularly in regional and remote locations. We audited current antivenom holdings in hospitals and health services across South Australia (SA) and compared to recommendations in the 'Snakebite and Spiderbite Management Guidelines' from the State's Toxinology service. The process also assessed the feasibility of 'real-time' remote stock monitoring. Fifty-three sites listed in the guideline were recommended to hold antivenom, though only 49 are currently operational. Interrogation of antivenom stock for 29 sites was possible using electronic reports generated from the State Pharmacy database. The 20 remaining centres had their stock levels confirmed by calling the centres directly. Data obtained were then compared to recommended levels of antivenom holdings in the guideline with discrepancies and associated costs documented. A separate report verification process was used to determine 'real-time' accuracy of the electronic reports. Thirty-seven sites (75%) held more than the recommended number of antivenom vials, totalling 129 vials in excess with an approximate total cost of $110 000. Twelve sites (24%) held inadequate stock to deliver a treatment dose for 19 envenoming events. The report verification revealed variances in the electronic reports. This audit has demonstrated a significant disparity between recommended and actual antivenom holdings across most sites in SA and has also revealed that 'real-time' remote monitoring of state antivenom holdings is not currently feasible. Correction of stock levels to that recommended may result in financial benefit for State Health while also addressing inequity in regional and remote healthcare provision.

Assessing phenotypic diversity between different landraces of okra (Abelmoschus esculentus L.)

The objective of the present study was to investigate the morphological variations among collections of okra landraces in order to improve local varieties in Palestine. Eighteen okra accessions from various locations in the West-Bank region were evaluated for morphological variation. Different sixteen morphological traits were used for okra collection evaluation. The results revealed significant differences in seed color, leaf rib color, stem color, branching patterns, and fruit characteristics. Cluster and similarity analysis classified into two main clusters (I and II) with further sub-clusters. Genetic proximity did not strictly align with geographical boundaries, suggesting that factors beyond location influence genetic similarity. Cluster I exhibited greater diversity, with sub-clusters hinting at potential local adaptation. In contrast, Cluster II was smaller and less diverse, implying potentially distinct genetic origins. These findings provide insights for breeding strategies and germplasm conservation.

Natural Diversity of Cuticular Pheromones in a Local Population of Drosophila after Laboratory Acclimation.

Experimental studies of insects are often based on strains raised for many generations in constant laboratory conditions. However, laboratory acclimation could reduce species diversity reflecting adaptation to varied natural niches. Hydrocarbons covering the insect cuticle (cuticular hydrocarbons; CHCs) are reliable adaptation markers. They are involved in dehydration reduction and protection against harmful factors. CHCs can also be involved in chemical communication principally related to reproduction. However, the diversity of CHC profiles in nature and their evolution in the laboratory have rarely been investigated. Here, we sampled CHC natural diversity in Drosophila melanogaster flies from a particular location in a temperate region. We also measured cis-Vaccenyl acetate, a male-specific volatile pheromone. After trapping flies using varied fruit baits, we set up 21 D. melanogaster lines and analysed their pheromones at capture and after 1 to 40 generations in the laboratory. Under laboratory conditions, the broad initial pheromonal diversity found in male and female flies rapidly changed and became more limited. In some females, we detected CHCs only reported in tropical populations: the presence of flies with a novel CHC profile may reflect the rapid adaptation of this cosmopolitan species to global warming in a temperate area.

Soil Color Comparison Using Munsell Soil Color Chart and Calibrated Smartphone Camera

Soil color is a crucial property in soil fertility assessment and monitoring. However, the subjective nature of the Munsell Soil Color Chart (MSCC) can lead to uncertainty in the analysis. To address this issue, a study was conducted to develop a soil color classification model from smartphone digital imagery based on color analysis and MSCC. The study involved taking 26 soil samples from various soil types and locations in the Special Region of Yogyakarta, Indonesia. Digital images of the soil were taken through a smartphone camera and compared with observations using MSCC to compare color differences (ΔE) based on Lab values. Soil images obtained from indoor studio conditions and calibration using spydercheckr in indoor and outdoor conditions are compared with MSCC and Chromameter values. The L*a*b color space was found to be superior to RGB for predicting and detecting small differences in color. The study also found that the Munsell soil color chart (MSCC) had a lower color difference than the chromameter in all lighting conditions, indicating that the MSCC or visual assessment can better detect the main soil color or soil matrix, while chromameter readings may have errors due to soil impurities.

Social media behaviors and barriers impacting women e-commerce entrepreneurs in rural Australia: A pilot study

ABSTRACT E-commerce businesses have proven to offer lifelines for residents in regional, rural, and remote (RRR) locations traditionally reliant on agricultural income. Prolonged droughts and frequent floods have had devastating impacts on the ability to farm, propelling the need to generate off-farm income to survive. This ever-growing necessity to overcome climate hardship has catalyzed a new generation of women e-commerce entrepreneurs in RRR Australia, in turn, supporting community development. Our study focuses on interviews with successful women e-commerce entrepreneurs from rural Queensland, Australia, to explore the common barriers they face and their use of social media to connect with consumers throughout Australia and globally. Results identified their three greatest challenges to be product supply, product distribution, and unreliable internet connectivity. Data exploring social media behaviors suggested a strong reliance on Facebook and Instagram to drive website traffic and generate sales. This study provides a unique contribution to knowledge regarding an under-researched segment of entrepreneurs, and how they contribute to community development.