Platform Location Research Articles

Novel Fuzzy Composite Indicators for Locating a Logistics Platform under Sustainability Perspectives

The purpose of this paper is to help decision-makers choose the location of a logistics platform with sustainability perspectives. This study presents a compensatory and partially compensatory approach to build composite indicators, using mainly fuzzy multi-criteria decision-making methods. In the first instance, the fuzzy full consistency method (F-FUCOM) was used to calculate the weight of the criteria and sub-criteria. In the second instance, two aggregation methods, namely the fuzzy multi-attribute ideal-real comparative analysis (F-MAIRCA) and the fuzzy preference ranking organization method for enrichment evaluation (F-PROMETHEE), were used to rank the location of a logistics platform. The novelty of the work lays in studying the impact of limited sustainability and weak sustainability on the location of a logistics platform. In this respect, the aggregation of various sustainability criterion in fuzzy compensatory and partially compensatory composite indicators is an innovative and interesting approach used to locate a logistics platform. The obtained results show that economic sustainability is the most important criterion for the selection of a logistics platform, followed by the environmental criterion. Obviously, the F-MAIRCA and F-PROMETHEE methods provided the same ranking orders. Finally, sensitivity analyses were performed to validate the robustness of the proposed approach.

A role for medial entorhinal cortex in spatial and nonspatial forms of memory in rats

Many studies have focused on the role of the medial entorhinal cortex (MEC) in spatial memory and spatial processing. However, more recently, studies have suggested that the functions of the MEC may extend beyond the spatial domain and into the temporal aspects of memory processing. The current study examined the effect of MEC lesions on spatial and nonspatial tasks that require rats to learn and remember information about location or stimulus-stimulus associations across short temporal gaps. MEC- and sham-lesioned male rats were tested on a watermaze delayed match to position (DMP) task and trace fear conditioning (TFC). Rats with MEC lesions were impaired at remembering the platform location after both the shortest (1 min) and the longest (6 h) delays on the DMP task, never performing as precisely as sham rats under the easiest condition and performing poorly at the longest delay. On the TFC task, although MEC-lesioned rats were not impaired at remembering the conditioning context, they showed reduced freezing in response to the previously associated tone. These findings suggest that the MEC plays a role in bridging temporal delays during learning and memory that extend beyond its established role in spatial memory processing.

Incline Impact: A Case Study

An incline impact test can be used as a shock test in lieu of a drop test in several test protocols, including ISTA Procedure 1A [1]. Some test protocols, such as ISTA Procedure 1E [2], only allow for an incline impact test and horizontal impact test. In this case study, a graph was developed for a 500-lb impact tester at Christian Brothers University (CBU) Packaging Laboratory. It determines sliding platform location on the incline for a given packaged-product weight to meet the impact velocity recommended by the International Safe Transit Association (ISTA). One station of the platform location higher than the station obtained from the graph is recommended to ensure the meeting of ISTA recommended impact velocity. It is well known that weight is not used in impact velocity of a free fall drop. However, this case study shows that weight contributes to impact velocity of an incline impact test. It contributes to the rolling friction. A heavier weight yields a smaller coefficient of rolling friction ( 𝜇𝑘 ), which results in a higher impact velocity. The coefficient of rolling friction for CBU’s incline impact tester can be computed from 𝜇𝑘 = −9 −5𝑤 + 0.1092, where w is the total weight of the sliding platform and packaged product.

Incline Impact: A Case Study

An incline impact test can be used as a shock test in lieu of a drop test in several test protocols, including ISTA Procedure 1A [1]. Some test protocols, such as ISTA Procedure 1E [2], only allow for an incline impact test and horizontal impact test. In this case study, a graph was developed for a 500-lb impact tester at Christian Brothers University (CBU) Packaging Laboratory. It determines sliding platform location on the incline for a given packaged-product weight to meet the impact velocity recommended by the International Safe Transit Association (ISTA). One station of the platform location higher than the station obtained from the graph is recommended to ensure the meeting of ISTA recommended impact velocity. It is well known that weight is not used in impact velocity of a free fall drop. However, this case study shows that weight contributes to impact velocity of an incline impact test. It contributes to the rolling friction. A heavier weight yields a smaller coefficient of rolling friction ( 𝜇𝑘 ), which results in a higher impact velocity. The coefficient of rolling friction for CBU’s incline impact tester can be computed from 𝜇𝑘 = −9 −5𝑤 + 0.1092, where w is the total weight of the sliding platform and packaged product.

Cognitive impairment resulting from treatment with docetaxel, doxorubicin, and cyclophosphamide

Breast cancer is the most commonly diagnosed cancer among women and it is estimated that about 30% of newly diagnosed cancers in women will be breast cancers. While advancements in treating breast cancer have led to an average 5-year survival rate of 90%, many survivors experience cognitive impairments as a result of chemotherapy treatment. Doxorubicin, cyclophosphamide, and docetaxel (TAC) are commonly administered as breast cancer treatments; however, there are few studies that have tested the cognitive effects of TAC. In the current study, 12-week-old female C57BL/6 mice received 4 weekly intraperitoneal injections of either saline or a combination therapy of doxorubicin and cyclophosphamide followed by 4 weekly docetaxel injections. Four weeks after the last injection, mice were tested for hippocampus-dependent cognitive performance in the Y-maze and the Morris water maze. During Y-maze testing, mice exposed to TAC exhibited impairment. During the water maze assessment, all animals were able to locate the visible and hidden platform locations. However, mice that received the TAC presented with a significant impairment in spatial memory retention on the probe trial days. TAC treatment significantly decreases the dendritic complexity of arborization in the dentate gyrus region of the hippocampus. In addition, comparative proteomic analysis revealed downregulation of proteins within key metabolic and signaling pathways associated with cognitive dysfunction, such as oxidative phosphorylation, ephrin signaling, and calcium signaling.

Controls and timing of Cenomanian-Turonian organic enrichment and relationship to the OAE2 event in Morocco, North Africa

The Cenomanian-Turonian Oceanic Anoxic Event (OAE2) is a significant global event that has been linked to major source rock development in marine environments. The aim of this study is to integrate biostratigraphic, sedimentological and geochemical data (including stable carbon isotopes and trace elements) to document the timing and controls of organic matter enrichment across the Cenomanian-Turonian boundary.Morocco has excellent exposures of Tethyan carbonate platform and basinal facies and this paper details a high-resolution analysis of organic-rich Late Cretaceous intervals exposed in the Errachidia-Goulmima Basin, to investigate their relationship to the OAE2. Integrating planktic foraminiferal and ammonite biostratigraphy with carbon isotope correlations indicate that organic-rich black mudstones identified were deposited from latest Cenomanian to early Turonian, suggesting a late-OAE2 to post-OAE2 age.The sedimentology, redox water conditions and palaeoproductivity of the palaeoenvironments were studied to characterise the lithofacies and geochemical signatures, and to analyse the controlling factors for organic–enrichment. In the study area during the lower OAE2 interval, a shallow water mid-ramp environment prevailed, with predominantly bivalve-rich limestone deposition. Subsequent organic-rich mudstone development can be correlated with the latest Cenomanian (late OAE2 interval) and early Turonian (post-OAE interval) transgression, which allowed development of anoxic to euxinic conditions in the outer-ramp environment, characterized by high TOC values, from 2.7 to 17 wt%. Shallow platform locations continued to experience limestone deposition with no organic enrichment.Trace and major element data from the C/T black mudstones show an extremely high concentration in palaeoproductivity-sensitive (P, Zn, Ni and Cu) and redox-sensitive elements (V, Mo, and U). The redox environments evolved from dysoxia to anoxia and sulfidic euxinia, which is interpreted to have provided optimum conditions for organic carbon preservation in the Errachidia area.The results highlight the important interplay of local paleogeography and eustatic sea-level (the late Cenomanian/early Turonian transgressions) as the main controls on organic mudstone deposition, rather than simply global OAE2 control. The development of organic-rich mudstones reflects both increased productivity at this time and enhanced organic matter preservation due to the change in redox bottom water conditions.

On-Line Precision Calibration of Mobile Manipulators Based on the Multi-Level Measurement Strategy

Autonomous industrial mobile manipulator (AIMM), which is composed of a mobile platform and a robot arm of six degrees of freedom (DoFs), can move autonomously and perform dexterous tasks independently in the industrial environment. A novel on-line calibration system for AIMM in real manufacturing workshop is proposed in this paper. Compared to calibration scenarios of fixed-base robot, we focus on the large-space autonomous calibration for AIMM. To cope with the issue of high precision calibration in large space, we propose a multi-level guided-based measurement strategy, which includes the low-cost vision positioning and guidance system, and the high-precision distributed base station (BS) measurement system. The corresponding experiment proves that the total uncertainty of measurement system is less than 0.1 mm. Based on the measured data of end-effector installed at the end of robot arm, the AIMM can perform on-line calibration tasks anywhere automatically without taking into account the expensive devices for the high-precision location of mobile platform and the downtime for the robotic off-line calibration. The mean positioning error of AIMM after calibration reduces 93% based on the calibration experiment.

Mutant Cx30-A88V mice exhibit hydrocephaly and sex-dependent behavioral abnormalities, implicating a functional role for Cx30 in the brain.

ABSTRACTConnexin 30 (Cx30; also known as Gjb6 when referring to the mouse gene) is expressed in ependymal cells of the brain ventricles, in leptomeningeal cells and in astrocytes rich in connexin 43 (Cx43), leading us to question whether patients harboring GJB6 mutations exhibit any brain anomalies. Here, we used mice harboring the human disease-associated A88V Cx30 mutation to address this gap in knowledge. Brain Cx30 levels were lower in male and female Cx30A88V/A88V mice compared with Cx30A88V/+ and Cx30+/+ mice, whereas Cx43 levels were lower only in female Cx30 mutant mice. Characterization of brain morphology revealed a disrupted ependymal cell layer, significant hydrocephalus and enlarged ventricles in 3- to 6-month-old adult male and female Cx30A88V/A88V mice compared with Cx30A88V/+ or Cx30+/+ sex-matched littermate mice. To determine the functional significance of these molecular and morphological changes, we investigated a number of behavioral activities in these mice. Interestingly, only female Cx30A88V/A88V mice exhibited abnormal behavior compared with all other groups. Cx30A88V/A88V female mice demonstrated increased locomotor and exploratory activity in both the open field and the elevated plus maze. They also exhibited dramatically reduced ability to learn the location of the escape platform during Morris water maze training, although they were able to swim as well as other genotypes. Our findings suggest that the homozygous A88V mutation in Cx30 causes major morphological changes in the brain of aging mice, possibly attributable to an abnormal ependymal cell layer. Remarkably, these changes had a more pronounced consequence for cognitive function in female mice, which is likely to be linked to the dysregulation of both Cx30 and Cx43 levels in the brain.

Link Budget Analysis for Reconfigurable Smart Surfaces in Aerial Platforms

Non-terrestrial networks, including Unmanned Aerial Vehicles (UAVs), High Altitude Platform Station (HAPS) nodes and Low Earth Orbiting (LEO) satellites, are expected to have a pivotal role in sixth-generation wireless networks. With inherent features such as flexible placement, wide footprints, and preferred channel conditions, they can tackle several challenges faced by current terrestrial networks. However, their successful and widespread adoption relies on energy-efficient on-board communication systems. In this context, the integration of Reconfigurable Smart Surfaces (RSS) into aerial platforms is envisioned as a key enabler of energy-efficient and cost-effective aerial platform deployments. RSS consist of low-cost reflectors capable of smartly directing signals in a nearly passive way. In this paper, we investigate the link budget of RSS-assisted communications for two RSS reflection paradigms discussed in the literature, namely “specular” and “scattering” paradigms. Specifically, we analyze the characteristics of RSS-equipped aerial platforms and compare their communication performance with that of RSS-assisted terrestrial networks using standardized channel models. In addition, we derive the optimal aerial platform placements for both reflection paradigms. Our results provide important insights for the design of RSS-assisted communications. For instance, given that a HAPS has a large area for RSS, it provides superior link budget performance in most studied scenarios. In contrast, the limited RSS area on UAVs and the large propagation loss in LEO satellite communications make them unfavorable candidates for supporting terrestrial users. Finally, the optimal location of an RSS-equipped platform may depend on the platform’s altitude, coverage footprint, and type of environment

Coastal chalk cliff retreat rates during the Holocene, inferred from submarine platform morphology and cosmogenic exposure along the Normandy coast (NW France)

Submerged marine terraces potentially provide crucial information on past sea-level variations and paleo-coastline locations that may be used to estimate long-term coastal erosion rates. The Normandy coastline has recently been surveyed using a shallow water high-resolution mapping system. We identified a new continuous submarine platform, called the inner platform, limited by a shore parallel edge located between -9 m and -10 m (NGF) along the Normandy chalk coastline. A lower rock platform, called the outer platform, ranging from about -14 m to -17 m (NGF) appears locally. This corresponds to inherited preserved submarine terraces created during a past sea level highstand. The high cosmogenic 10Be concentration measured at the end of Mesnil-Val inner shore platform (including intertidal and subtidal shore platforms) is attributed to the last glacial cliff location at 6.5 ky ± 1 ky. From the spatial edge location of the inner platform in Normandy, we estimated cliff retreat rates since 6.5 ky ± 1 ky ranging from 0.051 ± 0.008 m/y to 0.090 ± 0.014 m/y from place to place. Comparisons with the current coastal chalk cliffs indicate a mean retreat rate estimated over the contemporary period suggesting such long-term retreat rates are 33% to 57% lower than the contemporary ones (0.10 m/y to 0.18 m/y). This confirms a contemporary acceleration of chalk cliff system retreat rates.

Sodium bicarbonate cotransporter NBCn1/Slc4a7 affects locomotor activity and hearing in mice

Despite a widespread expression pattern in the central nervous system, the role of the sodium bicarbonate cotransporter NBCn1/Slc4a7 has not been investigated for locomotor activity, emotion and cognition. Here, we addressed the behavioral consequences of NBCn1 knockout and evaluated hearing and vision that are reportedly impaired in an earlier line of NBCn1 knockout mice and may contribute to behavioral changes. In a circular open field, the knockout mice traveled a shorter distance, especially in the periphery of the chamber, than wildtype littermates. The knockout mice also traveled a shorter total distance in a home cage-like open field. Rearing and grooming behaviors were reduced. The knockout and control mice displayed similar time spent and number of open and closed arms in the elevated plus maze test, indicating negligible change in anxiety. In the Morris water maze test, both groups of mice learned the location of an escape platform within comparable time on the training trials and showed similar platform identification on the probe trial. The knockout mice maintained normal visual responses in the optokinetic drum and produced evoked potentials in response to light stimuli. However, these mice failed to produce auditory evoked potentials. qPCR revealed a robust expression of an alternatively transcribed NBCn1 variant in the knockout mouse retina. These results indicate that NBCn1 deletion leads to reduced locomotor activity in mice by affecting their exploratory behaviors or emotionality. The deletion also causes hearing loss, but its effect on vision varies between different lines of knockout mice.

Agmatine improves the behavioral and cognitive impairments associated with chronic gestational ethanol exposure in rats

Chronic maternal ethanol exposure leads to poor intelligence, impaired cognition and array of neurological symptoms in offsprings and commonly referred as fetal alcohol spectrum disorder (FASD). Despite high prevalence and severity, the neurochemical basis of FASD remains largely unexplored. The present study evaluated the pharmacological effects of agmatine in cognitive deficits associated with FAS in rat’s offsprings prenatally exposed to alcohol. Pregnant rats received ethanol in liquid modified diet during the entire gestational period of 21 days. Offsprings were treated with agmatine (20–80 mg/Kg, i.p.) during early postnatal days (PND: 21–35) and subsequently evaluated for anxiety in elevated plus maze (EPM), depression in forced swim test (FST) and learning and memory in Morris’s water maze (MWM) during post adolescent phase. Hippocampal agmatine, BDNF, TNF-α and IL-6 levels were also analyzed in prenatally ethanol exposed pups. Offsprings prenatally exposed to ethanol demonstrated delayed righting reflex, reduced exploratory behavior along with anxiety, depression-like behavior and impaired memory. These behavioral abnormalities were correlated with a significant reduction in hippocampal agmatine and BDNF levels and elevation in TNF-α and IL-6 immunocontent. Chronic agmatine (40 and 80 mg/Kg, i.p.) administration for 15 days (PND: 21–35), improved entries and time spent in open arm of EPM, decreased immobility time in FST. It also reduced latency to reach the platform location; increased the number of entries, time spent in platform quadrant and also number of crossing over platform quadrant when subjected to MWM test in prenatally ethanol exposed offsprings. This study provides functional evidences for the therapeutic potential of agmatine in cognitive impairment and other neurological complications associated with FASD.

D-serine reduces memory impairment and neuronal damage induced by chronic lead exposure.

Although exogenous D-serine has been applied as a neural regulatory intervention in many studies, the role played by D-serine in hippocampal injuries caused by lead exposure remains poorly understood. Rat models of chronic lead exposure were established through the administration of 0.05% lead acetate for 8 weeks. Simultaneously, rats were administered 30 or 60 mg/kg D-serine, intraperitoneally, twice a day. Our results showed that D-serine treatment shortened the escape latency from the Morris water maze, increased the number of times that mice crossed the original platform location, and alleviated the pathological damage experienced by hippocampal neurons in response to lead exposure. Although D-serine administration did not increase the expression levels of the N-methyl-D-aspartate receptor subtype 2B (NR2B) in the hippocampi of lead-exposed rats, 60 mg/kg D-serine treatment restored the expression levels of NR2A, which are reduced by lead exposure. These findings suggested that D-serine can alleviate learning and memory impairments induced by lead exposure and that the underlying mechanism is associated with the increased expression of NR2A in the hippocampus. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China (approval No. LX2018155) on December 21, 2018.

An analysis of use and performance data aggregated from 35 institutional repositories

PurposeThis study demonstrates that aggregated data from the Repository Analytics and Metrics Portal (RAMP) have significant potential to analyze visibility and use of institutional repositories (IR) as well as potential factors affecting their use, including repository size, platform, content, device and global location. The RAMP dataset is unique and public.Design/methodology/approachThe webometrics methodology was followed to aggregate and analyze use and performance data from 35 institutional repositories in seven countries that were registered with the RAMP for a five-month period in 2019. The RAMP aggregates Google Search Console (GSC) data to show IR items that surfaced in search results from all Google properties.FindingsThe analyses demonstrate large performance variances across IR as well as low overall use. The findings also show that device use affects search behavior, that different content types such as electronic thesis and dissertation (ETD) may affect use and that searches originating in the Global South show much higher use of mobile devices than in the Global North.Research limitations/implicationsThe RAMP relies on GSC as its sole data source, resulting in somewhat conservative overall numbers. However, the data are also expected to be as robot free as can be hoped.Originality/valueThis may be the first analysis of aggregate use and performance data derived from a global set of IR, using an openly published dataset. RAMP data offer significant research potential with regard to quantifying and characterizing variances in the discoverability and use of IR content.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/OIR-08-2020-0328

Eestlaste, liivlaste ja lätlaste 19. sajandi II poole saunadest / Sauna in Estonia, Livonia and Latvia

Eestlaste, liivlaste ja lätlaste 19. sajandi II poole saunadest / Sauna in Estonia, Livonia and Latvia

Combined well path, submarine pipeline network, route and flow rate optimization for shallow-water offshore fields

This paper presents an integrated approach to planning wellbore trajectory, submarine pipeline networks, routes and production for offshore shallow-water oil and gas fields. The method integrates the optimization of realistic drilling well paths, platform location and well allocation using a combination of Constrained Optimization by Linear Approximation (COBYLA) and Mixed Integer Linear Programming (MILP). It also optimizes the pipeline network layout using a separate MILP routine that compares and combines three types of pipeline connections, namely: star connections from multiple wellhead platforms to a central facility; subsea connections from platforms to a subsea tie-in point; or piggyback connections between the wellhead platforms themselves. Specific pipeline paths are found using a global graph-search method that is coupled with a local refinement to find the least-cost path. We demonstrate this pipeline path optimization by considering shallow-water offshore fields that include the presence of obstacles along the route. The pipeline network layout and route optimization steps are further integrated with techniques to estimate the production flow rate and optimal operating pressure across the resulting pipeline network. A case study is provided based on real conditions of Gulf of Mexico to test and validate the model, and to highlight the importance of the subsurface and surface integration for field development planning.

Multilevel Completions in One Trip: Development and Deployment

This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper OTC 30747, “Tripping the Light Fantastic: When Three Become One,” by Euan Murdoch, SPE, Paul Day, and Steven Walduck, Weatherford, prepared for the 2020 Offshore Technology Conference, originally scheduled to be held in Houston, 4-7 May. The paper has not been peer reviewed. Copyright 2020 Offshore Technology Conference. Reproduced by permission. Typically, the completion of certain deepwater wells will necessitate the separate deployment of sandface, intermediate, and upper completions. What if this could be achieved in a single trip? In the simplest terms, completing in a single trip reduces the overall completion time by half and more. The complete paper describes the hurdles that have prevented single-trip installation of upper and lower completions in the complex world of subsea and deepwater applications and examines the processes, technologies, and risk-mitigation steps that took a concept from pilot to successful deployment. Introduction Almost universally in offshore operations, operators have elected to deploy completions in multiple trips. Simple completions on land are often deployed in a single-trip fashion, and the deployment of cemented single-trip gas-lift systems on platform locations in the Gulf of Thailand is commonplace. Subsea single-trip systems have been deployed in the Campos Basin; however, these tend to be relatively simple and in wells not requiring sand-control solutions. They typically consist of a safety valve, gas-lift mandrels, polished bore receptacle (PBR), production packer, and re-entry guide. At the time of writing, no one has run standalone screens as a single-trip system in deepwater applications.

Anti-Lingo-1 antibody ameliorates spatial memory and synapse loss induced by chronic stress.

Chronic stress can induce cognitive impairment, and synapse number was significantly decreased in the hippocampus of rats suffering from chronic stress. Lingo-1 is a potent negative regulator of axonal outgrowth and synaptic plasticity. In the current study, the effects of anti-Lingo-1 antibody on the spatial learning and memory abilities and hippocampal synapses of stressed rats were investigated. After 4 weeks of stress exposure, the model group was randomly divided into a chronic stress group and an anti-Lingo-1 group. Then, the anti-Lingo-1 group rats were treated with anti-Lingo-1 antibody (8 mg/kg) for 3 weeks. The effects of anti-Lingo-1 antibody on the spatial learning and memory abilities were investigated with the Morris water maze test. Immunohistological staining and an unbiased stereological method were used to estimate the total number of dendritic spine synapses in the hippocampus. At the behavioral level, after 3 weeks of treatment, the anti-Lingo-1 group rats displayed significantly more platform location crossings in the Morris water maze test than the chronic stress group rats. Anti-Lingo-1 significantly prevented the declines in dendritic spine synapses and postsynaptic density protein-95 (PSD-95) expression in the dentate gyrus and the CA1 and CA3 regions of the hippocampus. The present results indicated that anti-Lingo-1 antibody may be a safe and effective drug for alleviating memory impairment in rats after chronic stress and protecting synapses in the hippocampus of stressed rats.

Beta amyloid-induced time-dependent learning and memory impairment: involvement of HPA axis dysfunction.

Aβ aggregation is one of the pathological biomarkers of Alzheimer's disease (AD). However, the possible mechanism related to Aβ-induced pathological signaling pathway is still unknown. In the present study, Aβ1-42-induced time-dependent memory impairment and its possible relationship to hypothalamic-pituitary-adrenal (HPA) axis hyperactivity were examined. Aβ1-42-treated mice significantly impaired acquisition activity in the learning curve at 10days, 1 and 4months in the Morris water-maze (MWM) task. This learning activity was back to normal at 8months after Aβ1-42 treatment. In the probe trial test, Aβ1-42-treated mice needed longer latencies to touch the precious platform location and fewer numbers of crossing from 10days to 4months after microinjection. This Aβ1-42 induced memory loss was consistent with the results of the step-down passive avoidance test. The HPA axis related parameters, such as corticosterone (CORT) level in the serum, glucocorticoid receptor (GR) and corticotropin-releasing factor receptor (CRF-R) expression in the frontal cortex and hippocampus increased in Aβ1-42-treated mice from 10days to 4months. While the downstream molecules phosphorylation of cyclic AMP response element binding (pCREB) and brain-derived neurotrophic factor (BDNF) expression decreased during this time. These effects were back to normal 8months after treatment with Aβ1-42. Altogether, our results suggested that Aβ1-42 induced significant learning and memory impairment, which is involved in HPA axis dysfunction.

Dexmedetomidine ameliorates postoperative cognitive dysfunction by inhibiting Toll-like receptor 4 signaling in aged mice.

Our study aimed to explore the molecular mechanisms involved in the improvement of postoperative cognitive dysfunction (POCD) by dexmedetomidine (DEX). BV2 microglia cells were cultured under normal condition, DEX exposure (0.1 μg/mL), and lipopolysacchride (LPS) treatment (0.1 μg/mL) or with pretreatment of DEX before LPS incubation. For BV2 microglia cells, LPS induced markedly increased release of pro-inflammatory cytokines (interleukin [IL]-1β, IL-6, and tumor necrosis factor-alpha [TNF-α]) and expressions of Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB), while DEX pretreatment inhibited the LPS-induced production of pro-inflammatory cytokines and expressions of TLR4 and NF-κB. The spatial memory function was impaired in the aged mice following partial hepatectomy since the percentage of time spent in the target quadrant and the number of crossings over the former platform location were reduced. Pretreatment of DEX may attenuate neuroinflammation and improve POCD in aged mice through inhibiting the TLR4-NF-κB signaling pathway in the hippocampus.